##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933397.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269990
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.56378754768695	31.0	28.0	31.0	16.0	33.0
2	28.82277491758954	31.0	30.0	33.0	16.0	34.0
3	29.002718619208117	31.0	30.0	33.0	16.0	34.0
4	31.97499166635801	35.0	30.0	37.0	19.0	37.0
5	33.59819622948998	35.0	33.0	37.0	28.0	37.0
6	32.617411755990965	35.0	33.0	37.0	17.0	37.0
7	31.93789769991481	35.0	31.0	35.0	25.0	37.0
8	32.777217674728696	35.0	32.0	35.0	27.0	37.0
9	32.79528871439683	35.0	31.0	37.0	25.0	39.0
10	31.445749842586764	35.0	27.0	39.0	15.0	39.0
11	31.957620652616765	35.0	27.0	39.0	15.0	39.0
12	32.87278788103263	35.0	31.0	39.0	21.0	39.0
13	32.95943183080855	35.0	31.0	39.0	22.0	39.0
14	33.23920515574651	36.0	31.0	40.0	18.0	41.0
15	33.833804955739105	36.0	31.0	40.0	23.0	41.0
16	34.06383940145931	36.0	32.0	40.0	24.0	41.0
17	32.074843512722694	35.0	27.0	39.0	18.0	41.0
18	32.83032334530908	36.0	30.0	39.0	21.0	39.0
19	32.57266195044261	36.0	30.0	37.0	19.0	39.0
20	32.21208563280121	34.0	30.0	36.0	23.0	39.0
21	32.55500944479425	35.0	31.0	37.0	23.0	39.0
22	32.82051187081003	34.0	30.0	37.0	25.0	39.0
23	33.86279862217119	35.0	32.0	38.0	27.0	40.0
24	32.14417571021149	34.0	30.0	37.0	21.0	39.0
25	31.322223045297974	34.0	29.0	37.0	18.0	39.0
26	30.63924219415534	33.0	25.0	37.0	18.0	39.0
27	32.24447572132301	34.0	30.0	37.0	22.0	39.0
28	32.260576317641394	35.0	31.0	37.0	21.0	39.0
29	32.49485166117264	35.0	31.0	38.0	21.0	40.0
30	31.531456720619282	35.0	30.0	37.0	18.0	40.0
31	31.80755583540131	35.0	30.0	37.0	20.0	40.0
32	31.548449942590466	35.0	30.0	37.0	18.0	40.0
33	31.531101151894514	35.0	30.0	38.0	15.0	40.0
34	30.659283677173228	35.0	29.0	38.0	10.0	40.0
35	30.131015963554205	35.0	27.0	38.0	9.0	40.0
36	29.446868402533426	34.0	24.0	38.0	8.0	40.0
37	29.589247749916662	35.0	24.0	38.0	8.0	40.0
38	29.53585688358828	35.0	23.0	38.0	8.0	40.0
39	29.0480943738657	34.0	23.0	37.0	7.0	40.0
40	28.961657839179228	34.0	23.0	38.0	7.0	40.0
41	28.393777547316567	33.0	21.0	37.0	7.0	40.0
42	28.78899588873662	34.0	22.0	38.0	7.0	40.0
43	28.782558613281974	34.0	21.0	38.0	7.0	40.0
44	28.72999740731138	34.0	21.0	38.0	7.0	40.0
45	28.97809178117708	34.0	22.0	38.0	7.0	40.0
46	28.730930775213896	34.0	22.0	38.0	7.0	40.0
47	28.835093892366384	34.0	22.0	38.0	7.0	40.0
48	28.57239157005815	34.0	22.0	37.0	7.0	40.0
49	28.387906959517018	34.0	21.0	37.0	7.0	39.0
50	28.56810992999741	34.0	22.0	37.0	7.0	40.0
51	26.957953998296233	32.0	20.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	13.0
10	11.0
11	20.0
12	26.0
13	45.0
14	100.0
15	171.0
16	384.0
17	620.0
18	1061.0
19	1606.0
20	2396.0
21	3265.0
22	4607.0
23	6331.0
24	8417.0
25	11445.0
26	14540.0
27	15640.0
28	15571.0
29	16144.0
30	17556.0
31	19537.0
32	21975.0
33	23998.0
34	26017.0
35	27316.0
36	19304.0
37	9910.0
38	1928.0
39	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.656024297196193	2.405274269417386	34.552390829289976	40.38631060409645
2	40.73743471980444	11.523389755176119	34.15015370939665	13.5890218156228
3	13.199377754731657	11.535612430090003	61.57005815030187	13.694951664876475
4	12.144894255342791	2.9952961220785954	68.57476202822326	16.285047594355344
5	19.38442164524612	3.3619763694951663	60.84447572132301	16.4091262639357
6	14.164228304752028	10.958183636430979	62.4767583984592	12.400829660357791
7	53.00307418793289	1.2833808659579984	42.95936886551354	2.7541760805955775
8	54.198674024963886	8.286603207526205	34.24645357235453	3.268269195155376
9	50.29297381384495	2.823808289195896	34.433867921034114	12.449349975925033
10	28.336234675358345	19.1458942923812	39.781843771991554	12.736027260268898
11	24.443868291418198	15.496870254453867	47.12026371347087	12.93899774065706
12	19.959257750287048	14.184229045520205	49.003296418385865	16.853216785806882
13	17.752509352198228	15.425386125412052	54.42683062335642	12.395273899033297
14	15.262417126560242	17.58176228749213	50.2537131004852	16.902107485462427
15	12.868254379791843	14.662765287603245	56.409126263935704	16.05985406866921
16	15.07722508241046	14.801288936627282	48.80551131523389	21.31597466572836
17	15.696136893959038	15.669469239601467	52.00118522908256	16.63320863735694
18	16.243564576465793	14.102374162006	51.19078484388311	18.463276417645098
19	16.326900996333197	17.4350901885255	47.75658357716953	18.481425237971777
20	18.253639023667542	17.142486758768843	51.625986147635096	12.977888069928516
21	17.94140523723101	20.253342716396904	47.46657283603096	14.338679210341123
22	15.859476276899146	14.714248675876885	51.078928849216645	18.347346198007333
23	15.569095151672286	20.065557983629024	49.1466350605578	15.218711804140895
24	15.961702285269824	16.42060817067299	48.518093262713435	19.099596281343754
25	15.377606578021407	21.17004333493833	46.27578799214786	17.176562094892404
26	15.304270528538094	16.62431941923775	49.58072521204489	18.490684840179267
27	15.68391421904515	17.517685840216306	48.11215230193711	18.686247638801436
28	12.744916478388088	19.29849253676062	50.855587243972	17.101003740879293
29	17.263972739731102	17.479165895033148	47.70991518204378	17.54694618319197
30	15.133893847920293	19.474795362791212	49.784806844697954	15.606503944590541
31	17.785103151968592	18.934034593873847	45.48835142042298	17.792510833734582
32	20.781510426312085	19.729989999629616	44.25904663135672	15.229452942701583
33	17.48842549724064	18.08474387940294	42.124893514574616	22.301937108781807
34	18.410681877106562	24.269787769917404	41.553761250416684	15.765769102559354
35	17.809918885884663	22.29156635430942	41.839327382495654	18.05918737731027
36	19.885551316715432	26.117263602355646	36.64283862365273	17.354346457276197
37	20.908922552687137	22.594540538538467	39.915922811956	16.5806140968184
38	19.824437942146005	24.00162968998852	36.26578762176377	19.90814474610171
39	21.884884625356495	24.012000444460906	35.94355346494314	18.159561465239456
40	22.58083632727138	21.115967258046595	37.31841920071114	18.984777213970887
41	21.514130152968626	24.532019704433498	35.52835290195933	18.425497240638542
42	21.146338753287157	21.559687395829474	39.33812363420868	17.95585021667469
43	21.73302714915367	21.27338049557391	36.40060742990482	20.592984925367606
44	21.420793362717138	21.441164487573612	37.67472869365532	19.46331345605393
45	18.678099188858845	22.208970702618615	36.843586799511094	22.269343309011443
46	21.748583280862256	23.409015148709212	35.37501388940331	19.467387681025222
47	16.856920626689877	21.63265306122449	42.028593651616724	19.481832660468907
48	19.634060520760027	24.326826919515536	35.59650357420645	20.442608985517982
49	19.399977776954703	21.005963183821624	38.66920997073966	20.924849068484015
50	19.78702914922775	20.918922923071225	37.046186895810955	22.24786103189007
51	19.68554390903367	20.843734953146413	36.13022704544613	23.340494092373792
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	38.0
1	265.5
2	493.0
3	12694.0
4	24895.0
5	16814.0
6	8733.0
7	8265.5
8	7798.0
9	7628.0
10	7458.0
11	6946.5
12	6435.0
13	5917.5
14	5400.0
15	4907.5
16	4415.0
17	4039.0
18	3663.0
19	3308.5
20	2954.0
21	2798.5
22	2643.0
23	2523.5
24	2404.0
25	2410.0
26	2499.0
27	2582.0
28	2835.0
29	3088.0
30	3452.0
31	3816.0
32	4114.0
33	4412.0
34	4808.5
35	5205.0
36	5522.0
37	5839.0
38	6061.5
39	6284.0
40	6699.5
41	7115.0
42	7820.0
43	8525.0
44	9453.0
45	10381.0
46	18394.0
47	26407.0
48	22082.0
49	17757.0
50	16887.0
51	16017.0
52	14517.0
53	13017.0
54	12290.5
55	11564.0
56	11106.0
57	10648.0
58	9707.5
59	8767.0
60	8078.5
61	7390.0
62	6595.5
63	5801.0
64	5234.5
65	4668.0
66	4071.5
67	3475.0
68	3023.0
69	2571.0
70	2171.0
71	1771.0
72	1463.0
73	1155.0
74	997.0
75	698.5
76	558.0
77	440.5
78	323.0
79	226.0
80	129.0
81	110.0
82	91.0
83	62.5
84	34.0
85	19.5
86	5.0
87	4.5
88	4.0
89	4.0
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	269990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.40712309588373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.68632431116409	38.573857099890326
2	7.259454578989007	7.028186228281953
3	3.5666311279940293	5.1795105615125285
4	2.448832272527956	4.741637010297339
5	1.7811963476680404	4.311129542975268
6	1.282713826509419	3.7255491657980095
7	0.9429781169376696	3.1952800448328498
8	0.7465857705327861	2.891205543665263
9	0.5324893788672282	2.3198651019068937
>10	1.6716464462790932	11.883917064077606
>50	0.04327883868302448	1.43058849459051
>100	0.030913456202160343	2.5229834747694557
>500	0.003864182025270043	1.4088902381349009
>1k	0.0015456728101080172	2.3407929421163236
>5k	7.728364050540086E-4	2.0819102961287106
>10k+	7.728364050540086E-4	6.364697191022057
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17013	6.301344494240528	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCG	5565	2.0611874513870885	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGC	3816	1.4133856809511463	No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC	2441	0.9041075595392422	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATAGCAGGT	967	0.35816141338568097	No Hit
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	940	0.3481610430015927	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT	656	0.24297196192451573	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTC	607	0.22482314159783695	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTA	596	0.22074891662654172	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT	458	0.16963591244120155	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCATAGCAGGTCGTATGCCG	457	0.16926552835290196	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	273	0.10111485610578169	No Hit
GAATCTTTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC	272	0.10074447201748214	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.088892181191896	0.0
2	0.0	0.0	0.0	0.6426163931997482	0.0
3	0.0	0.0	0.0	0.7900292603429757	0.0
4	0.0	0.0	0.0	1.2567132116004296	0.0
5	0.0	0.0	0.0	2.472313789399607	0.0
6	0.0	0.0	0.0	2.7352864920922997	0.0
7	0.0	0.0	0.0	3.0767806215045	0.0
8	0.0	0.0	0.0	3.513833845697989	0.0
9	0.0	0.0	0.0	3.6682840105189083	0.0
10	0.0	0.0	0.0	6.394681284492018	0.0
11	0.0	0.0	0.0	6.828401051890811	0.0
12	0.0	0.0	0.0	8.848475869476648	0.0
13	3.7038408829956663E-4	0.0	0.0	9.03440868180303	0.0
14	3.7038408829956663E-4	0.0	0.0	9.151079669617394	0.0
15	3.7038408829956663E-4	0.0	0.0	9.553687173599021	0.0
16	3.7038408829956663E-4	0.0	0.0	9.789992221934146	0.0
17	3.7038408829956663E-4	0.0	0.0	10.020371124856476	0.0
18	3.7038408829956663E-4	0.0	0.0	10.224823141597836	0.0
19	3.7038408829956663E-4	0.0	0.0	10.870032223415683	0.0
20	3.7038408829956663E-4	0.0	0.0	11.100040742249712	0.0
21	3.7038408829956663E-4	0.0	0.0	11.31671543390496	0.0
22	3.7038408829956663E-4	0.0	0.0	11.651172265639469	0.0
23	3.7038408829956663E-4	0.0	0.0	11.885995777621394	0.0
24	3.7038408829956663E-4	0.0	0.0	12.06748398088818	0.0
25	3.7038408829956663E-4	0.0	0.0	12.215637616208008	0.0
26	3.7038408829956663E-4	0.0	0.0	12.352309344790548	0.0
27	3.7038408829956663E-4	0.0	0.0	12.52972332308604	0.0
28	3.7038408829956663E-4	0.0	0.0	12.668987740286678	0.0
29	3.7038408829956663E-4	0.0	0.0	12.84529056631727	0.0
30	3.7038408829956663E-4	0.0	0.0	13.06085410570762	0.0
31	3.7038408829956663E-4	0.0	0.0	13.203822363791252	0.0
32	3.7038408829956663E-4	0.0	0.0	13.367902514907959	0.0
33	3.7038408829956663E-4	0.0	0.0	13.519759991110782	0.0
34	3.7038408829956663E-4	0.0	0.0	13.712730101114856	0.0
35	3.7038408829956663E-4	0.0	0.0	13.930145560946702	0.0
36	3.7038408829956663E-4	0.0	0.0	14.097559168858107	0.0
37	3.7038408829956663E-4	0.0	0.0	14.25756509500352	0.0
38	3.7038408829956663E-4	0.0	0.0	14.46164672765658	0.0
39	3.7038408829956663E-4	0.0	0.0	14.853142708989221	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTTG	75	0.0	45.000004	1
GCTACGA	35	1.2073178E-7	45.000004	10
CACCGGT	105	0.0	45.000004	16
CCACCGG	105	0.0	45.000004	15
AATGCGA	25	3.882851E-5	45.0	25
TGCGAAT	25	3.882851E-5	45.0	27
CCCCCGT	25	3.882851E-5	45.0	16
TAATGCG	25	3.882851E-5	45.0	24
CTACGAA	30	2.1593114E-6	44.999996	11
CGGTGTC	60	0.0	44.999996	19
CCGGTGA	30	2.1593114E-6	44.999996	18
CGTTTTT	7605	0.0	44.1716	1
TACGGCT	465	0.0	43.54839	7
CCGATGA	115	0.0	43.043476	18
ACCACCG	305	0.0	42.78688	14
GATACCT	690	0.0	42.717392	5
TGATACC	680	0.0	42.683823	4
ACCGGTG	95	0.0	42.63158	17
CGACCAC	535	0.0	41.635513	12
GACCACC	540	0.0	41.250004	13
>>END_MODULE