FastQCFastQC Report
Sat 14 Jan 2017
SRR2933396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853905
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC275713.2288135096995565No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254982.986046457158583No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG238982.7986719834173592No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC191142.238422306931099No Hit
GAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT62930.7369672270334522No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTC50350.5896440470544148No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCATGTTT45510.5329632687476944No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC45070.5278104707198108TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT41560.4867051955428297No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTA35590.4167910950281355No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTT29370.3439492683612346No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGT14910.17460958771760324No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTAT9600.1124246842447345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT266000.044.50941
CGATGAA8250.043.90909219
CCGATGA8250.043.0909118
ACGGCTG21600.042.0833368
TACGGCT21700.041.9930887
CGGCTGT21700.041.88949
ATACCTG28600.041.7744756
GATACCT28650.041.701575
AGACACG1350.041.66666824
TGATACC29150.041.0634654
GAATCTG33400.040.6212541
TACCTGT29500.040.423737
GAATGAC6200.040.2822571
TCGTTCA451.9272193E-840.016
TACGGTG900.040.07
CGACCTG3050.039.83606712
AACACGT1300.039.8076941
GATGAAT9400.039.49468220
AATCTGT34250.039.4160582
GAATGAT95850.039.2488251
Produced by FastQC (version 0.11.5)