##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933394.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	717821
Sequences flagged as poor quality	0
Sequence length	51
%GC	32
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.37562985758288	31.0	28.0	33.0	25.0	34.0
2	28.460719315818288	31.0	27.0	33.0	16.0	34.0
3	28.38141960182274	31.0	26.0	33.0	16.0	34.0
4	33.20212142024265	35.0	32.0	37.0	28.0	37.0
5	34.35061247859843	35.0	33.0	37.0	32.0	37.0
6	33.72296436019565	35.0	33.0	37.0	28.0	37.0
7	34.099728205221076	35.0	33.0	37.0	30.0	37.0
8	33.694969915898255	35.0	33.0	37.0	28.0	37.0
9	35.166480222785346	37.0	34.0	39.0	30.0	39.0
10	35.16176316936952	37.0	34.0	39.0	28.0	39.0
11	35.66280590843678	37.0	34.0	39.0	30.0	39.0
12	35.74255977465134	37.0	35.0	39.0	30.0	39.0
13	35.91626881910671	37.0	35.0	39.0	30.0	39.0
14	36.75572461658268	38.0	36.0	41.0	31.0	41.0
15	36.99161768741789	39.0	36.0	41.0	31.0	41.0
16	36.96516123100327	39.0	35.0	41.0	31.0	41.0
17	36.07540319940487	38.0	34.0	40.0	27.0	41.0
18	35.75371018680144	38.0	34.0	39.0	29.0	40.0
19	34.956408352500134	37.0	34.0	37.0	27.0	40.0
20	33.581422109411676	35.0	32.0	37.0	27.0	39.0
21	34.02816997552315	35.0	33.0	37.0	28.0	39.0
22	34.33597233850779	35.0	33.0	38.0	28.0	40.0
23	34.569105668404795	35.0	33.0	38.0	29.0	40.0
24	33.979046308202186	35.0	33.0	38.0	26.0	40.0
25	33.15571430760593	35.0	33.0	37.0	23.0	40.0
26	32.900805353981006	35.0	31.0	37.0	23.0	40.0
27	33.79015102650939	35.0	33.0	38.0	25.0	40.0
28	33.328027460885096	35.0	33.0	38.0	23.0	40.0
29	33.18132793551596	35.0	33.0	38.0	22.0	40.0
30	32.374505621875095	35.0	31.0	38.0	19.0	40.0
31	32.086400370008676	35.0	31.0	38.0	17.0	40.0
32	30.937898166813174	35.0	29.0	38.0	12.0	40.0
33	29.491997308521206	35.0	22.0	38.0	7.0	40.0
34	28.16056649220349	35.0	17.0	38.0	7.0	40.0
35	27.245511067522404	34.0	15.0	38.0	7.0	40.0
36	26.479682260619292	33.0	10.0	38.0	7.0	40.0
37	26.41463958284865	33.0	10.0	38.0	7.0	40.0
38	26.184140614442878	33.0	10.0	38.0	7.0	40.0
39	25.86099598646459	33.0	10.0	38.0	7.0	40.0
40	25.622021367443974	31.0	10.0	38.0	7.0	40.0
41	25.172352160218217	31.0	10.0	38.0	7.0	40.0
42	25.304022869211128	31.0	10.0	38.0	7.0	40.0
43	25.271410281950516	31.0	10.0	38.0	7.0	40.0
44	25.439356051160388	32.0	10.0	38.0	7.0	40.0
45	25.516795970025953	32.0	10.0	38.0	7.0	40.0
46	25.28952203961712	31.0	10.0	38.0	7.0	40.0
47	25.130383480004067	31.0	10.0	38.0	7.0	40.0
48	24.937590290615628	31.0	10.0	38.0	7.0	40.0
49	25.016051355421478	31.0	8.0	38.0	7.0	40.0
50	24.964665285635277	31.0	8.0	38.0	7.0	40.0
51	23.870346506998263	28.0	8.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
7	1.0
8	9.0
9	25.0
10	21.0
11	25.0
12	59.0
13	102.0
14	130.0
15	243.0
16	510.0
17	1066.0
18	2134.0
19	4154.0
20	6675.0
21	10309.0
22	15121.0
23	22463.0
24	33155.0
25	47948.0
26	57999.0
27	52907.0
28	41054.0
29	33592.0
30	30339.0
31	31324.0
32	34393.0
33	38981.0
34	44190.0
35	50509.0
36	54846.0
37	55863.0
38	41813.0
39	5861.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.586880294669562	1.859655819487031	23.471729024366798	49.081734861476605
2	49.19499429523516	17.950993353496205	22.95962363876231	9.894388712506322
3	7.892636186458741	18.135161830038406	63.29948552633595	10.672716457166898
4	7.006203496414844	1.8912793022215844	79.87144427371169	11.23107292765188
5	19.025773834981145	2.3129721755145085	66.3492709185159	12.31198307098845
6	8.972431845822287	14.694053252830441	69.1335305041229	7.1999843972243776
7	37.26904061040287	0.7065828388971623	59.98278122261678	2.041595328083185
8	35.93542122618313	11.242357077878747	49.758365943598754	3.0638557523393715
9	33.81859822992083	1.6733976854954091	48.518781144602904	15.98922293998086
10	22.21960628067443	8.42842435648999	57.009755914078866	12.342213448756723
11	18.079159010393955	12.69522624721205	57.326547983410904	11.89906675898309
12	11.581856758161157	10.62618675129315	61.494857353016975	16.297099137528715
13	12.415072838493161	11.998952385065358	67.50930942393717	8.076665352504316
14	11.667532713587372	18.10938938816223	58.154052333381166	12.069025564869236
15	8.615239732468122	12.83021811844457	67.53856462822905	11.015977520858264
16	8.825180650886502	14.296182474460903	57.96709764690641	18.911539227746193
17	9.707851957521443	15.646658428772634	62.68275795776385	11.962731655942079
18	10.063233034419444	14.189888565533748	60.784652441207484	14.962225958839321
19	10.149884163322053	16.828568682164498	57.58956620104455	15.431980953468901
20	12.037959324121195	14.587202101916773	63.9507620980718	9.42407647589023
21	11.755716257952887	19.64695933944535	59.011229819133185	9.586094583468581
22	10.103354457448305	11.7562734999394	61.730570713311536	16.40980132930076
23	11.156681122452532	18.608817518573574	59.861163159060546	10.373338199913348
24	12.90433130265066	14.202008578740383	57.48438677609041	15.40927334251854
25	9.092378183418987	22.93245809191985	56.2265523020363	11.74861142262486
26	10.233609771795475	12.872847130412735	61.90763435452571	14.98590874326608
27	13.88479857791845	15.544404524247687	56.185177084537784	14.385619813296072
28	9.894945954492833	15.181918612021661	61.869323967953015	13.053811465532494
29	13.276290328647391	12.029461383826886	60.40154857548051	14.292699712045204
30	10.659342649490611	17.355998222398064	57.132349151111484	14.852309976999836
31	16.270490832672767	13.086410121743445	54.546885644192635	16.096213401391154
32	13.954453826232447	17.6677751138515	55.099112452825985	13.278658607090069
33	12.99321139949932	14.75228504042094	48.24461808723902	24.009885472840722
34	18.899279904042928	18.087517640191635	48.41778103454761	14.595421421217825
35	12.732282839315094	21.981663952433824	43.31135478064866	21.974698427602423
36	17.981641662754363	18.61174303900276	45.56762758403558	17.838987714207303
37	14.537886186110466	21.13827820584798	46.15342822235627	18.170407385685287
38	19.034411085772078	21.712098141458664	38.813436776020765	20.440053996748492
39	21.300435623922954	20.478643004314446	42.68612927178224	15.534792099980358
40	15.99395949686621	24.38769553969583	40.55203177393807	19.06631318949989
41	19.680254548139438	23.564927746610927	40.944608753435745	15.810208951813893
42	16.2019500683318	24.912478180493466	42.50251803722655	16.383053713948183
43	18.1580087514854	20.98225044962463	41.68016817563153	19.179572623258444
44	18.842859152908595	22.00409294239093	37.649915508183796	21.50313239651668
45	16.76657551116504	21.7346664419124	38.69000767600836	22.808750370914197
46	21.120307151782967	23.373375813747437	37.65911000096124	17.847207033508354
47	15.214795889225865	20.427376741555346	44.83680471872514	19.52102265049365
48	19.649188307391398	22.814880032765828	35.50913110650148	22.026800553341292
49	15.73762818307071	21.856284505468633	41.82505109212464	20.581036219336017
50	17.694940660693963	20.192638554737183	40.037279488897646	22.075141295671205
51	17.61135436271717	20.78122540299044	36.89095192255451	24.716468311737884
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	40.0
1	1006.5
2	1973.0
3	25996.5
4	50020.0
5	39320.0
6	28620.0
7	28126.0
8	27632.0
9	27804.0
10	27976.0
11	27689.5
12	27403.0
13	26877.0
14	26351.0
15	25197.5
16	24044.0
17	23086.5
18	22129.0
19	20788.0
20	19447.0
21	18200.0
22	16953.0
23	15970.5
24	14988.0
25	13975.5
26	12311.0
27	11659.0
28	11529.5
29	11400.0
30	11179.5
31	10959.0
32	11080.5
33	11202.0
34	12176.5
35	13151.0
36	13654.0
37	14157.0
38	14731.5
39	15306.0
40	16578.5
41	17851.0
42	19070.0
43	20289.0
44	23018.0
45	25747.0
46	68675.0
47	111603.0
48	75745.0
49	39887.0
50	35843.5
51	31800.0
52	26937.0
53	22074.0
54	19492.0
55	16910.0
56	15067.5
57	13225.0
58	11761.0
59	10297.0
60	8699.0
61	7101.0
62	5962.0
63	4823.0
64	3947.0
65	3071.0
66	2500.5
67	1930.0
68	1552.5
69	1175.0
70	919.5
71	664.0
72	535.0
73	406.0
74	323.0
75	211.0
76	182.0
77	134.5
78	87.0
79	60.5
80	34.0
81	34.0
82	34.0
83	22.0
84	10.0
85	8.5
86	7.0
87	3.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	717821.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.718011961266335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.52302306270212	48.20890833932559
2	6.161981724472107	6.866667428584828
3	2.519312190775136	4.2111320033931925
4	1.4812454477336934	3.3012820629758894
5	0.97275674450139	2.7100035962765467
6	0.6537902378292524	2.1856735374917666
7	0.433878366975743	1.6922388028622404
8	0.2882512917939939	1.284863113922258
9	0.21140578117629047	1.0601198859835264
>10	0.6705303546692584	5.957499980193616
>50	0.04847701082813767	1.8907294698530523
>100	0.029793134371837764	3.491797702320174
>500	0.0025248418959102187	0.9625268876852756
>1k	0.0017673893271371532	3.270509366399779
>5k	2.524841895910219E-4	0.7562912230628532
>10k+	0.0010099367583640876	12.149756599669518
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26434	3.6825336678642726	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTC	21376	2.977901175919902	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCG	21189	2.951850113050468	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGC	17366	2.4192660844416647	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCT	5376	0.7489332298720712	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTC	4362	0.60767238629129	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGTCGATT	4252	0.5923482316622111	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC	3743	0.5214391888785644	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCT	3624	0.5048612397798337	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTA	3249	0.4526198035443377	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTT	2584	0.3599783232867247	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCGTCGATTCGT	1434	0.19977125216453684	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTAT	906	0.12621530994495841	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATG	825	0.11493115971809129	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	776	0.1081049453833198	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	759	0.10573666694064397	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	728	0.1014180415451763	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.061157308019687356	0.0
2	0.0	0.0	0.0	0.6581027860706221	0.0
3	0.0	0.0	0.0	0.7623070375483582	0.0
4	0.0	0.0	0.0	1.6623921562617978	0.0
5	0.0	0.0	0.0	5.205336706504825	0.0
6	0.0	0.0	0.0	5.706715183868959	0.0
7	0.0	0.0	0.0	6.383485576487732	0.0
8	0.0	0.0	0.0	7.177137475777387	0.0
9	0.0	0.0	0.0	7.310875552540257	0.0
10	0.0	0.0	0.0	10.894498767798657	0.0
11	0.0	0.0	0.0	11.072955513979112	0.0
12	0.0	0.0	0.0	14.090002939451479	0.0
13	0.0	0.0	0.0	14.323765952793245	0.0
14	0.0	0.0	0.0	14.51322822820731	0.0
15	0.0	0.0	0.0	15.111984742714409	0.0
16	0.0	0.0	0.0	15.427801638570061	0.0
17	0.0	0.0	0.0	15.593023887570856	0.0
18	0.0	0.0	0.0	15.719935749998955	0.0
19	0.0	0.0	0.0	16.55719183473317	0.0
20	0.0	0.0	0.0	16.730215471545133	0.0
21	0.0	0.0	0.0	16.849883188148578	0.0
22	0.0	0.0	0.0	17.117080720681063	0.0
23	0.0	0.0	0.0	17.25207259191358	0.0
24	0.0	0.0	0.0	17.367560993618188	0.0
25	0.0	0.0	0.0	17.465774893740917	0.0
26	0.0	0.0	0.0	17.61664816158903	0.0
27	0.0	0.0	0.0	17.7747655752618	0.0
28	0.0	0.0	0.0	17.871864991411506	0.0
29	0.0	0.0	0.0	17.98414925169367	0.0
30	0.0	0.0	0.0	18.09908041141176	0.0
31	0.0	0.0	0.0	18.21693709155904	0.0
32	0.0	0.0	0.0	18.3561082776904	0.0
33	0.0	0.0	0.0	18.468810469462444	0.0
34	0.0	0.0	0.0	18.635565133926146	0.0
35	0.0	0.0	0.0	18.76066595989808	0.0
36	0.0	0.0	0.0	18.884373680903735	0.0
37	0.0	0.0	0.0	19.03246073881929	0.0
38	0.0	0.0	0.0	19.179711933755073	0.0
39	0.0	0.0	0.0	19.332674859052606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	100	0.0	45.000004	17
CGACGAA	25	3.8893355E-5	45.000004	19
ACCGACG	30	2.1643573E-6	45.000004	17
CGGCGAA	20	7.0315157E-4	45.0	31
ACTGCGA	20	7.0315157E-4	45.0	30
ATACCGG	20	7.0315157E-4	45.0	28
CGTTTTT	28370	0.0	44.49242	1
CGATGAA	630	0.0	43.57143	19
GATACCT	2500	0.0	42.929996	5
CGGCTGT	2290	0.0	42.740173	9
ACGGCTG	2290	0.0	42.34716	8
TGATACC	2525	0.0	42.148514	4
TACGGCT	2310	0.0	42.077923	7
ATACCTG	2560	0.0	41.92383	6
CCGATGA	675	0.0	41.666668	18
GAATGAC	455	0.0	41.53846	1
GATACCG	60	3.6379788E-12	41.250004	27
TACCTGT	2600	0.0	41.19231	7
CGCTTTT	870	0.0	41.12069	1
GAATCTG	2565	0.0	41.05263	1
>>END_MODULE