FastQCFastQC Report
Sat 14 Jan 2017
SRR2933393.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933393.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394087
Sequences flagged as poor quality0
Sequence length51
%GC31

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218865.553596033363192No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCG87572.222098166140979No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGC61481.5600616107610756No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTC55981.420498519362476No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGTCGATT18800.4770520215079411No Hit
GAACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCT18770.47629076828213057No Hit
GCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC15830.40168795215269726No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTC13020.33038390000177625No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTA9800.24867605376477786No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTT8190.2078221306462786No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCT7790.19767208763547137No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTCGTCGATTCGTATGCCG6970.17686449946331648No Hit
GAATCTTTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTC6320.16037067957075468No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCGTCGATTCGT5200.13195055914049436No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4910.1245917779576591No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4580.1162179924737431No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT4570.11596424139847293No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTCGCTTCGTATGCCG4270.10835170914036747No Hit
GAATGACTCTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCT4080.10353043871023403No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCG207.0276385E-445.01
CGGGTAG207.0276385E-445.06
ACCGGTG750.045.017
GCGACCG253.8861243E-545.011
GGACTCA207.0276385E-445.08
CCGTGTC253.8861243E-545.019
CGACCGC253.8861243E-545.012
TCCCGAG207.0276385E-445.045
TCGAGGG207.0276385E-445.03
TTCGCTG406.7957444E-945.01
TGCGTTG253.8861243E-545.01
GGGCAAC302.161858E-644.9999967
CGTTTTT176450.044.7067151
CCGATGA1900.043.8157918
CGATGAA1650.043.63636419
TACGGCT8700.043.1896557
CACCGGT1050.042.85714316
ACGGCTG8750.042.6857158
GATACCT11400.041.8421065
AATGATC2800.041.7857132
Produced by FastQC (version 0.11.5)