##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933393.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	394087
Sequences flagged as poor quality	0
Sequence length	51
%GC	31
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.506865235341436	31.0	27.0	31.0	16.0	33.0
2	28.55561335441159	31.0	28.0	33.0	16.0	34.0
3	28.637552114127082	31.0	28.0	33.0	16.0	34.0
4	32.24196180031313	35.0	32.0	37.0	19.0	37.0
5	33.90848974972531	35.0	33.0	37.0	30.0	37.0
6	32.75548038884815	35.0	33.0	37.0	17.0	37.0
7	33.01305549282265	35.0	32.0	37.0	28.0	37.0
8	33.07707181409181	35.0	32.0	37.0	27.0	37.0
9	33.78717389814939	35.0	32.0	39.0	27.0	39.0
10	33.10121622890377	37.0	31.0	39.0	17.0	39.0
11	33.889511706805855	37.0	32.0	39.0	25.0	39.0
12	34.39440275878169	37.0	32.0	39.0	27.0	39.0
13	34.705905041272615	37.0	33.0	39.0	27.0	39.0
14	34.91933253317161	37.0	33.0	40.0	25.0	41.0
15	35.329280082824354	38.0	32.0	40.0	27.0	41.0
16	35.39597094042686	38.0	32.0	40.0	27.0	41.0
17	33.52255466432539	36.0	31.0	40.0	19.0	41.0
18	33.8637255225369	37.0	32.0	39.0	25.0	39.0
19	33.45421696224438	37.0	32.0	37.0	25.0	39.0
20	32.37618596908804	35.0	31.0	35.0	24.0	39.0
21	32.746956890229825	35.0	32.0	36.0	25.0	39.0
22	32.55505763955675	34.0	30.0	37.0	25.0	39.0
23	33.49298758903491	35.0	32.0	37.0	27.0	39.0
24	31.852184923633615	34.0	31.0	36.0	21.0	39.0
25	31.239695803210967	34.0	29.0	36.0	18.0	39.0
26	29.777490757117082	33.0	24.0	36.0	18.0	39.0
27	31.97638846244611	34.0	30.0	37.0	22.0	39.0
28	31.953926417263194	35.0	31.0	37.0	21.0	39.0
29	31.78602440577842	35.0	30.0	37.0	21.0	39.0
30	31.01383450862373	34.0	27.0	36.0	18.0	39.0
31	31.214432346157068	34.0	30.0	36.0	18.0	39.0
32	30.628952490186176	34.0	27.0	37.0	15.0	39.0
33	29.47567161565847	34.0	23.0	37.0	9.0	40.0
34	27.670265702750914	33.0	18.0	37.0	7.0	39.0
35	26.71066541144468	33.0	15.0	37.0	7.0	39.0
36	25.820790840601187	32.0	10.0	37.0	7.0	39.0
37	25.76885560802564	33.0	10.0	37.0	7.0	40.0
38	25.63843263035827	32.0	10.0	37.0	7.0	40.0
39	25.170804923785866	31.0	10.0	37.0	7.0	39.0
40	24.87829083425741	30.0	10.0	36.0	7.0	39.0
41	24.42100094649151	30.0	10.0	36.0	7.0	39.0
42	24.792193094418238	30.0	10.0	37.0	7.0	39.0
43	24.878473535031606	30.0	10.0	37.0	7.0	39.0
44	24.9625742539084	31.0	10.0	37.0	7.0	40.0
45	25.128948683920047	31.0	10.0	37.0	7.0	40.0
46	24.785032239074113	31.0	10.0	37.0	7.0	40.0
47	24.82185405760657	31.0	10.0	37.0	7.0	40.0
48	24.550913884497586	30.0	10.0	37.0	7.0	39.0
49	24.388122419668754	30.0	9.0	36.0	7.0	39.0
50	24.491866009282216	30.0	8.0	37.0	7.0	39.0
51	23.035695163758255	26.0	8.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
8	1.0
9	6.0
10	10.0
11	19.0
12	25.0
13	47.0
14	82.0
15	195.0
16	466.0
17	1033.0
18	1908.0
19	3209.0
20	5049.0
21	7456.0
22	10428.0
23	14728.0
24	20196.0
25	27720.0
26	32076.0
27	29296.0
28	23600.0
29	20913.0
30	21314.0
31	23238.0
32	25571.0
33	27969.0
34	30164.0
35	30128.0
36	22726.0
37	11964.0
38	2503.0
39	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.736824609794283	1.84629282366584	25.9541674807847	50.46271508575517
2	52.36204188415249	13.827149842547456	23.79804459421397	10.01276367908609
3	8.144902014022284	13.767518339858963	67.53711743853515	10.550462207583605
4	7.5310781629437145	1.918611880117842	78.72170358321894	11.828606373719508
5	16.91733043718773	2.358869995711607	68.87895312456386	11.8448464425368
6	9.358339655964292	11.445949752212076	70.84400145145615	8.351709140367483
7	37.76678753676219	0.7584619639825724	59.235143508920615	2.2396069903346216
8	38.29382852009836	8.890422673166078	49.592856399728994	3.2228924070065745
9	35.21836548782375	1.7140885134500756	49.29393763306072	13.77360836566545
10	21.069713032908975	9.026433249510895	57.17290852019985	12.730945197380276
11	16.840951363531403	13.260269940393874	58.50256415461561	11.39621454145912
12	11.884177859203678	11.120640873715702	61.834823275063634	15.16035799201699
13	11.988977053290263	12.24805690114112	67.24428869767335	8.518677347895261
14	11.384541991996691	15.766823061912724	60.105255946022076	12.743379000068513
15	8.690720576928443	12.997891328564503	66.87178211917673	11.43960597533032
16	8.857181282305683	12.6966888022188	59.70026922989086	18.745860685584656
17	9.842750458655068	14.537145351153423	63.28069690195313	12.339407288238384
18	10.274127286614378	12.900197164585487	61.87237843420361	14.953297114596523
19	10.514429554895239	16.22662001030229	58.5936607906376	14.665289644164867
20	11.910314219956506	13.966205431795517	64.13152425733404	9.991956090913936
21	11.990245808666614	18.197250860850524	59.367347819136384	10.44515551134648
22	10.259917226399248	12.251609416194901	61.89826104388118	15.59021231352468
23	10.925760047908204	18.101586705473665	59.54091355462119	11.431739691996945
24	11.88544661458003	13.598266372653752	59.09126664924243	15.425020363523789
25	9.534696653277068	19.665708333439063	57.646154275578745	13.153440737705127
26	10.024943730699059	13.552337428029851	62.42657078259369	13.996148058677399
27	11.94279435759109	15.156805476963209	58.27977071052839	14.620629454917317
28	9.994239850591367	14.617076939863532	62.74629713743411	12.64238607211098
29	12.990786298456939	12.02602471027971	60.84874659656371	14.134442394699647
30	10.654500148444379	16.357555565141706	58.91059588365005	14.077348402763857
31	14.461527530722911	13.069449131790694	56.692811485788674	15.776211851697722
32	13.308482644695207	16.767871053853593	56.973460175037495	12.95018612641371
33	12.078551182860638	14.159310000076125	52.18695364221606	21.575185174847178
34	16.770662315681562	17.26725316998531	50.56269300941163	15.399391504921503
35	12.162542788775067	20.23563324849589	45.598053221750526	22.003770740978513
36	17.23984805385613	17.48065782428753	46.332662584657704	18.946831537198637
37	14.44503371083035	20.299578519463974	46.85158353358522	18.40380423612045
38	18.13584310063514	20.73323910710072	41.620251365815164	19.51066642644898
39	20.016646070537725	19.682963406557434	44.01769152496784	16.282698997937004
40	16.41769456998074	22.62114710711087	41.986921669580575	18.974236653327818
41	18.720231826982367	21.510732401728553	43.535056979803954	16.23397879148513
42	16.060413056000325	22.997713702811815	44.30443024002314	16.637443001164716
43	17.2928820285876	19.74589367322444	44.06590422926917	18.895320068918792
44	18.424358073217334	20.7512554334449	40.1188062534415	20.705580239896268
45	16.675251911379974	20.52846198935768	41.09803165290913	21.698254446353214
46	19.188402560855852	22.148155102807248	39.77167478247189	18.89176755386501
47	15.331639967824366	19.17216249203856	45.318927038953326	20.17727050118375
48	18.860302420531507	21.040785410328176	38.39735895880859	21.701553210331728
49	15.414616569437714	20.539627036669568	42.058479472806766	21.98727692108595
50	17.111957511919957	18.737486900100738	41.69307792441771	22.457477663561598
51	17.469492776975642	19.18688005440423	39.1971823480602	24.146444820559925
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	38.0
1	197.5
2	357.0
3	16972.5
4	33588.0
5	25041.0
6	16494.0
7	16198.5
8	15903.0
9	15721.0
10	15539.0
11	15396.5
12	15254.0
13	14709.5
14	14165.0
15	13592.0
16	13019.0
17	12368.0
18	11717.0
19	11008.0
20	10299.0
21	9463.0
22	8627.0
23	8115.5
24	7604.0
25	6999.0
26	6087.0
27	5780.0
28	5717.5
29	5655.0
30	5562.5
31	5470.0
32	5621.0
33	5772.0
34	6261.0
35	6750.0
36	7162.5
37	7575.0
38	8000.5
39	8426.0
40	9241.0
41	10056.0
42	10836.0
43	11616.0
44	13405.5
45	15195.0
46	30047.5
47	44900.0
48	36165.5
49	27431.0
50	24507.0
51	21583.0
52	17901.0
53	14219.0
54	12094.5
55	9970.0
56	8718.0
57	7466.0
58	6556.0
59	5646.0
60	4809.0
61	3972.0
62	3358.0
63	2744.0
64	2292.0
65	1840.0
66	1467.0
67	1094.0
68	938.0
69	782.0
70	613.5
71	445.0
72	355.5
73	266.0
74	225.5
75	151.5
76	118.0
77	89.0
78	60.0
79	50.0
80	40.0
81	29.5
82	19.0
83	12.5
84	6.0
85	5.5
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	394087.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.47770057739575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.98741667269762	51.73811522859872
2	6.541843765568863	7.781876494252159
3	2.5154292048080293	4.4883583520162595
4	1.350639103854705	3.21331632828769
5	0.8139696223335885	2.4206520738126542
6	0.5037672619792349	1.797775102121725
7	0.329160728193102	1.3704406263314808
8	0.20070533868691595	0.9549993630964152
9	0.15264195998913535	0.8170913512602552
>10	0.5195073844096105	5.316284233900548
>50	0.03856013885537933	1.6255279352176168
>100	0.04029317880388206	4.699806354227901
>500	0.0025995599228311	1.140807255738947
>1k	0.0017330399485540667	1.7115974232252282
>5k	0.00129977996141555	5.283481175607777
>10k+	4.332599871385167E-4	5.63987070230463
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21886	5.553596033363192	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCG	8757	2.222098166140979	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGC	6148	1.5600616107610756	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTC	5598	1.420498519362476	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGTCGATT	1880	0.4770520215079411	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCT	1877	0.47629076828213057	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC	1583	0.40168795215269726	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTC	1302	0.33038390000177625	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTA	980	0.24867605376477786	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTT	819	0.2078221306462786	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCT	779	0.19767208763547137	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTCGTCGATTCGTATGCCG	697	0.17686449946331648	No Hit
GAATCTTTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTC	632	0.16037067957075468	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCGTCGATTCGT	520	0.13195055914049436	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	491	0.1245917779576591	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	458	0.1162179924737431	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT	457	0.11596424139847293	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTCGCTTCGTATGCCG	427	0.10835170914036747	No Hit
GAATGACTCTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCT	408	0.10353043871023403	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10606794946293585	0.0
2	0.0	0.0	0.0	0.6828441435520582	0.0
3	0.0	0.0	0.0	0.8901587720477966	0.0
4	0.0	0.0	0.0	1.6722195860304958	0.0
5	0.0	0.0	0.0	3.6129078096968437	0.0
6	0.0	0.0	0.0	4.176235196796647	0.0
7	0.0	0.0	0.0	4.660899750562693	0.0
8	0.0	0.0	0.0	5.334355104329755	0.0
9	0.0	0.0	0.0	5.581254900567641	0.0
10	0.0	0.0	0.0	8.697825607036009	0.0
11	0.0	0.0	0.0	9.262675500587434	0.0
12	0.0	0.0	0.0	11.622814251675392	0.0
13	0.0	0.0	0.0	11.915896743612452	0.0
14	0.0	0.0	0.0	12.080834942538068	0.0
15	0.0	0.0	0.0	12.547990672110473	0.0
16	0.0	0.0	0.0	12.875075808133737	0.0
17	0.0	0.0	0.0	13.162575776414853	0.0
18	0.0	0.0	0.0	13.434089426953946	0.0
19	0.0	0.0	0.0	14.24837662749596	0.0
20	0.0	0.0	0.0	14.510247737174787	0.0
21	0.0	0.0	0.0	14.800285216208604	0.0
22	0.0	0.0	0.0	15.203749425888192	0.0
23	0.0	0.0	0.0	15.495563162448901	0.0
24	0.0	0.0	0.0	15.738656692557734	0.0
25	0.0	0.0	0.0	15.94038879739753	0.0
26	0.0	0.0	0.0	16.144150910839485	0.0
27	0.0	0.0	0.0	16.3474055221309	0.0
28	0.0	0.0	0.0	16.54000258826097	0.0
29	0.0	0.0	0.0	16.75112348288576	0.0
30	0.0	0.0	0.0	16.97670818880095	0.0
31	0.0	0.0	0.0	17.197471624286006	0.0
32	0.0	0.0	0.0	17.41011502536242	0.0
33	0.0	0.0	0.0	17.622758426438832	0.0
34	0.0	0.0	0.0	17.840730600095917	0.0
35	0.0	0.0	0.0	18.061747786656245	0.0
36	0.0	0.0	0.0	18.272107428055225	0.0
37	0.0	0.0	0.0	18.494139618916634	0.0
38	0.0	0.0	0.0	18.699424238810213	0.0
39	0.0	0.0	0.0	18.899887588273657	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTTCG	20	7.0276385E-4	45.0	1
CGGGTAG	20	7.0276385E-4	45.0	6
ACCGGTG	75	0.0	45.0	17
GCGACCG	25	3.8861243E-5	45.0	11
GGACTCA	20	7.0276385E-4	45.0	8
CCGTGTC	25	3.8861243E-5	45.0	19
CGACCGC	25	3.8861243E-5	45.0	12
TCCCGAG	20	7.0276385E-4	45.0	45
TCGAGGG	20	7.0276385E-4	45.0	3
TTCGCTG	40	6.7957444E-9	45.0	1
TGCGTTG	25	3.8861243E-5	45.0	1
GGGCAAC	30	2.161858E-6	44.999996	7
CGTTTTT	17645	0.0	44.706715	1
CCGATGA	190	0.0	43.81579	18
CGATGAA	165	0.0	43.636364	19
TACGGCT	870	0.0	43.189655	7
CACCGGT	105	0.0	42.857143	16
ACGGCTG	875	0.0	42.685715	8
GATACCT	1140	0.0	41.842106	5
AATGATC	280	0.0	41.785713	2
>>END_MODULE