FastQCFastQC Report
Sat 14 Jan 2017
SRR2933390.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933390.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656469
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168502.5667624823106654No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCG141932.162021359729096No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC122821.8709185049103614No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC90461.377978244212598No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT29860.4548577312866259TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCCTTTT29600.4508971482278676No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTC28740.4377967581104363No Hit
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC26660.40611209364036993TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTA25650.3907267517582704No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCT23280.35462451387651206No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGT9870.1503498261151707No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTT8730.13298419270369202No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTAT7270.11074399552758775No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG253.888971E-545.04
ACCGGTG750.045.017
GCGATCG253.888971E-545.09
CTAGCGG253.888971E-545.02
TACGGTG302.1640735E-644.9999967
CGTTTTT167400.044.1935461
CCGATGA4700.042.1276618
GAATGAC3350.041.6417921
CACCGGT1150.041.08695616
TACGGCT10900.041.077987
ACGGCTG10900.041.077988
GATACCT16600.041.069285
TGATACC16700.040.958084
CGATGAA4850.040.82474519
ATACCTG16950.040.221246
GGCGATA451.9261279E-840.08
ATACGCC451.9261279E-840.06
GTACGGG451.9261279E-840.03
CGGCTGT11550.039.5454569
AATGATC4000.039.3752
Produced by FastQC (version 0.11.5)