##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933390.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	656469
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.478252590754476	31.0	28.0	33.0	25.0	34.0
2	28.834027197019203	31.0	28.0	33.0	16.0	34.0
3	28.839214037525	31.0	28.0	34.0	16.0	34.0
4	33.171296740592474	35.0	32.0	37.0	28.0	37.0
5	34.22483163713747	35.0	33.0	37.0	30.0	37.0
6	33.703582347376646	35.0	33.0	37.0	28.0	37.0
7	33.55019048881211	35.0	32.0	37.0	29.0	37.0
8	33.62425339201089	35.0	33.0	37.0	28.0	37.0
9	34.67462439201242	37.0	34.0	39.0	28.0	39.0
10	34.56118720000487	37.0	32.0	39.0	27.0	39.0
11	34.85056720119305	37.0	34.0	39.0	27.0	39.0
12	35.01637853424914	37.0	34.0	39.0	27.0	39.0
13	35.234390352019666	37.0	34.0	39.0	30.0	39.0
14	35.883124717237216	38.0	34.0	40.0	27.0	41.0
15	36.36216333139874	38.0	34.0	40.0	30.0	41.0
16	36.2135576851306	38.0	34.0	40.0	30.0	41.0
17	35.48420869835438	37.0	33.0	40.0	27.0	41.0
18	35.30074687456681	37.0	33.0	39.0	27.0	40.0
19	34.64534806670231	37.0	33.0	38.0	27.0	40.0
20	34.10831585345233	35.0	33.0	38.0	27.0	40.0
21	34.31062395939488	35.0	33.0	38.0	27.0	40.0
22	34.84843762614838	35.0	33.0	39.0	29.0	40.0
23	35.20739897847423	35.0	34.0	39.0	30.0	40.0
24	34.629204120834345	35.0	33.0	39.0	27.0	40.0
25	33.490108443810755	35.0	32.0	38.0	24.0	40.0
26	33.63606506933305	35.0	32.0	38.0	24.0	40.0
27	34.38505245487601	35.0	33.0	39.0	26.0	40.0
28	34.19342878338505	35.0	33.0	39.0	25.0	40.0
29	34.189318916811	35.0	33.0	39.0	24.0	40.0
30	33.398486447951086	35.0	33.0	39.0	21.0	40.0
31	33.34535065631431	35.0	33.0	39.0	21.0	40.0
32	32.75739143813341	35.0	32.0	39.0	18.0	40.0
33	32.168967613093685	35.0	31.0	39.0	13.0	40.0
34	31.630140951057857	36.0	30.0	40.0	10.0	41.0
35	31.125730232501457	36.0	27.0	40.0	8.0	41.0
36	30.54801521473215	35.0	24.0	40.0	7.0	41.0
37	30.449763812152593	36.0	23.0	40.0	7.0	41.0
38	30.239677730403113	35.0	23.0	39.0	7.0	41.0
39	29.849336373842483	35.0	22.0	39.0	7.0	40.0
40	29.83014277901927	35.0	22.0	39.0	7.0	40.0
41	29.388882034033596	35.0	21.0	39.0	7.0	40.0
42	29.564718212131876	35.0	21.0	39.0	7.0	40.0
43	29.455686407126613	35.0	21.0	39.0	7.0	40.0
44	29.436503475411634	35.0	20.0	39.0	7.0	40.0
45	29.534646723607665	35.0	20.0	39.0	7.0	40.0
46	29.30267689715737	35.0	20.0	39.0	7.0	40.0
47	29.312054339199566	35.0	20.0	39.0	7.0	40.0
48	29.104963067562977	35.0	20.0	39.0	7.0	40.0
49	29.270232105400254	35.0	20.0	39.0	7.0	40.0
50	29.269241959635565	35.0	20.0	39.0	7.0	40.0
51	28.279877648449506	34.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	15.0
9	41.0
10	39.0
11	50.0
12	62.0
13	99.0
14	139.0
15	171.0
16	390.0
17	667.0
18	1449.0
19	2601.0
20	4202.0
21	6472.0
22	8972.0
23	13027.0
24	18744.0
25	27147.0
26	33197.0
27	33710.0
28	29336.0
29	26730.0
30	26896.0
31	29525.0
32	34776.0
33	41902.0
34	49309.0
35	59760.0
36	68362.0
37	73852.0
38	56648.0
39	8176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.21154845087887	2.6505440470151678	34.639107101782415	37.49880040032355
2	38.30036147936917	13.723420298597496	33.52664025262427	14.449577969409066
3	12.690774431085094	14.01284752212214	58.37929894633258	14.91707910046019
4	11.315842789225385	3.0732601234787933	68.70469131063311	16.906205776662723
5	19.56406166932483	3.7747403152319454	59.61621950160632	17.044978513836906
6	13.98801771294608	12.847065131788401	62.345365889326075	10.819551265939442
7	54.50234512216113	1.3921449451535413	41.44978666167024	2.6557232710150824
8	54.17178876687247	9.007279856322233	33.63860288909301	3.182328487712291
9	50.70445062904723	3.1273373152426087	33.341711489803785	12.826500565906388
10	27.386974860960684	17.509890032888073	41.5836848350798	13.519450271071445
11	22.94457164009268	18.102911180878305	44.9712019912593	13.981315187769718
12	16.994557244896562	16.326589679025208	49.45960890765596	17.21924416842227
13	17.135310273600123	16.774592555017833	53.89683290452405	12.193264266857993
14	15.284956334571778	21.536432032586458	47.769506252389675	15.409105380452084
15	13.108920604019383	17.265857184421503	54.40104559392751	15.224176617631603
16	14.72087790893401	18.491962301342486	47.03497042510766	19.752189364615845
17	15.502636072685839	19.03882742368642	49.864045370002245	15.594491133625501
18	15.744840959740673	17.62733655359202	49.56106076600723	17.066761720660075
19	15.61718832115454	20.180389325314678	45.92707347947885	18.275348874051936
20	17.4183396321837	19.239598518741936	50.33946766717088	13.002594181903488
21	17.23325853924557	22.459095555159497	47.234218218986726	13.073427686608202
22	15.930074382796446	16.444340859964445	49.26508334742387	18.36050140981524
23	15.681319300682897	21.80300973846442	47.959157248857146	14.556513711995539
24	16.393157940435877	19.239903178977226	46.89680700840405	17.470131872182844
25	14.18589453576635	24.949235988294955	45.11439230184517	15.750477174093522
26	14.853862101637702	18.223861294288078	48.84328125166611	18.07899535240811
27	17.026241909366625	20.006428330964603	45.64495810160114	17.32237165806763
28	13.880015659536094	20.425336154487113	48.401219250261626	17.293428935715166
29	16.795614111252778	18.512831527459788	45.45073720160434	19.240817159683093
30	15.502636072685839	23.633560762198975	45.89081891147945	14.972984253635738
31	18.71908650675051	18.789920011455223	43.7511900790441	18.73980340275017
32	17.738689869590186	21.4066467723533	42.484869811064954	18.369793546991556
33	16.29079210137874	20.005971340611666	40.58714120544915	23.11609535256044
34	17.692990834296822	21.56293747305661	41.506910455786944	19.237161236859624
35	15.658926773389148	25.276441080995447	39.86113586475523	19.20349628086018
36	19.60062089755952	22.595735670686658	37.36002766314937	20.44361576860446
37	16.64511195501996	25.92475806169065	37.890441132787686	19.539688850501697
38	20.16683194484431	24.492702625714237	34.741777601074844	20.59868782836661
39	19.155664623919787	23.513676959612717	37.26070842644511	20.069949990022376
40	18.22294731358221	24.04287178830988	38.291526332545786	19.44265456556212
41	18.18440779381814	25.428009548051772	37.88358627749368	18.503996380636405
42	18.027507772644253	24.282944053717692	38.948373799829085	18.74117437380897
43	19.330844259211023	23.22089847349989	35.87511367635029	21.573143590938795
44	19.95417910061252	24.9688865734711	34.35364046131652	20.72329386459985
45	18.577876487694013	23.591365319611437	35.3940551648288	22.436703027865747
46	20.804485817304396	25.136145042644813	33.823836312148785	20.235532827902002
47	16.556608156668478	23.766392624785023	39.58663699275975	20.09036222578675
48	19.237618227212558	25.114057175586357	34.03070061191008	21.617623985291004
49	17.863600566058718	23.138335549736546	38.21109603042947	20.786967853775273
50	18.32272354063939	22.819660943624147	36.5275435702219	22.33007194551456
51	18.330187716403973	23.30087178526328	34.46895436037345	23.899986137959296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	53.0
1	847.5
2	1642.0
3	18497.0
4	35352.0
5	27257.0
6	19162.0
7	18403.5
8	17645.0
9	17341.5
10	17038.0
11	16563.5
12	16089.0
13	15501.0
14	14913.0
15	14169.5
16	13426.0
17	12652.5
18	11879.0
19	11233.5
20	10588.0
21	10198.5
22	9809.0
23	9418.0
24	9027.0
25	8909.0
26	9005.5
27	9220.0
28	9700.5
29	10181.0
30	10702.0
31	11223.0
32	12105.0
33	12987.0
34	14630.5
35	16274.0
36	17641.0
37	19008.0
38	20140.5
39	21273.0
40	22390.0
41	23507.0
42	24354.0
43	25201.0
44	26992.5
45	28784.0
46	55537.0
47	82290.0
48	61345.0
49	40400.0
50	38064.5
51	35729.0
52	31599.5
53	27470.0
54	26046.0
55	24622.0
56	23036.0
57	21450.0
58	19344.0
59	17238.0
60	15170.5
61	13103.0
62	11614.0
63	10125.0
64	8489.0
65	6853.0
66	5826.5
67	4800.0
68	4091.5
69	3383.0
70	2783.0
71	2183.0
72	1855.0
73	1527.0
74	1230.5
75	797.5
76	661.0
77	501.5
78	342.0
79	253.0
80	164.0
81	118.0
82	72.0
83	50.0
84	28.0
85	18.5
86	9.0
87	9.0
88	9.0
89	6.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	656469.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.95763135318978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.74211452617685	41.65384538055139
2	6.503953629431958	6.628521427736687
3	3.2614700193695736	4.985903607495463
4	2.116967769233164	4.315026526846724
5	1.6065344828753676	4.093259596727518
6	1.2057585238639534	3.6865559016015377
7	0.8989043357554969	3.2064225034248772
8	0.6462399075317873	2.6344683978979417
9	0.4835406229229647	2.2176076326480163
>10	1.4510620655515187	10.83332019191061
>50	0.04946553497847015	1.7188334237584337
>100	0.029133070550569424	2.724771977615249
>500	0.0018208169094103227	0.6699042115226038
>1k	0.0018208169094103227	2.5328626686354405
>5k	3.0346948490172045E-4	1.3988812320945232
>10k+	9.104084547051614E-4	6.699815319532967
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16850	2.5667624823106654	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCG	14193	2.162021359729096	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC	12282	1.8709185049103614	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC	9046	1.377978244212598	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT	2986	0.4548577312866259	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCCTTTT	2960	0.4508971482278676	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTC	2874	0.4377967581104363	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	2666	0.40611209364036993	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTA	2565	0.3907267517582704	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCT	2328	0.35462451387651206	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGT	987	0.1503498261151707	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTT	873	0.13298419270369202	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTAT	727	0.11074399552758775	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5233011764454986E-4	0.0	0.0	0.08241059364570147	0.0
2	1.5233011764454986E-4	0.0	0.0	0.6087111501076212	0.0
3	1.5233011764454986E-4	0.0	0.0	0.7115339795176924	0.0
4	1.5233011764454986E-4	0.0	0.0	1.3203974597429582	0.0
5	1.5233011764454986E-4	0.0	0.0	3.692025061350955	0.0
6	1.5233011764454986E-4	0.0	0.0	3.9404754832292155	0.0
7	1.5233011764454986E-4	0.0	0.0	4.439661278750406	0.0
8	1.5233011764454986E-4	0.0	0.0	5.070003305563553	0.0
9	1.5233011764454986E-4	0.0	0.0	5.185469534738122	0.0
10	1.5233011764454986E-4	0.0	0.0	7.883540578458389	0.0
11	1.5233011764454986E-4	0.0	0.0	8.129401388336692	0.0
12	1.5233011764454986E-4	0.0	0.0	10.007022418423414	0.0
13	1.5233011764454986E-4	0.0	0.0	10.215714679596447	0.0
14	1.5233011764454986E-4	0.0	0.0	10.38525810053483	0.0
15	1.5233011764454986E-4	0.0	0.0	10.938521087819836	0.0
16	1.5233011764454986E-4	0.0	0.0	11.209821027344779	0.0
17	1.5233011764454986E-4	0.0	0.0	11.382106390400764	0.0
18	3.0466023528909973E-4	0.0	0.0	11.526972332280732	0.0
19	3.0466023528909973E-4	0.0	0.0	12.22159766873988	0.0
20	3.0466023528909973E-4	0.0	0.0	12.422064103560107	0.0
21	3.0466023528909973E-4	0.0	0.0	12.5718046092047	0.0
22	3.0466023528909973E-4	0.0	0.0	12.809134932494908	0.0
23	3.0466023528909973E-4	0.0	0.0	12.950954272021985	0.0
24	3.0466023528909973E-4	0.0	0.0	13.085157105666832	0.0
25	3.0466023528909973E-4	0.0	0.0	13.194682460253263	0.0
26	3.0466023528909973E-4	0.0	0.0	13.318222185662995	0.0
27	3.0466023528909973E-4	0.0	0.0	13.470247643072256	0.0
28	3.0466023528909973E-4	0.0	0.0	13.592568727540828	0.0
29	3.0466023528909973E-4	0.0	0.0	13.725705250362164	0.0
30	3.0466023528909973E-4	0.0	0.0	13.87620740659498	0.0
31	3.0466023528909973E-4	0.0	0.0	14.022139659298459	0.0
32	3.0466023528909973E-4	0.0	0.0	14.174469776943008	0.0
33	3.0466023528909973E-4	0.0	0.0	14.330303487293383	0.0
34	3.0466023528909973E-4	0.0	0.0	14.519649823525558	0.0
35	3.0466023528909973E-4	0.0	0.0	14.690716545640388	0.0
36	3.0466023528909973E-4	0.0	0.0	14.84715957646134	0.0
37	3.0466023528909973E-4	0.0	0.0	15.021577561164351	0.0
38	3.0466023528909973E-4	0.0	0.0	15.198128167514383	0.0
39	3.0466023528909973E-4	0.0	0.0	15.414436934569645	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	25	3.888971E-5	45.0	4
ACCGGTG	75	0.0	45.0	17
GCGATCG	25	3.888971E-5	45.0	9
CTAGCGG	25	3.888971E-5	45.0	2
TACGGTG	30	2.1640735E-6	44.999996	7
CGTTTTT	16740	0.0	44.193546	1
CCGATGA	470	0.0	42.12766	18
GAATGAC	335	0.0	41.641792	1
CACCGGT	115	0.0	41.086956	16
TACGGCT	1090	0.0	41.07798	7
ACGGCTG	1090	0.0	41.07798	8
GATACCT	1660	0.0	41.06928	5
TGATACC	1670	0.0	40.95808	4
CGATGAA	485	0.0	40.824745	19
ATACCTG	1695	0.0	40.22124	6
GGCGATA	45	1.9261279E-8	40.0	8
ATACGCC	45	1.9261279E-8	40.0	6
GTACGGG	45	1.9261279E-8	40.0	3
CGGCTGT	1155	0.0	39.545456	9
AATGATC	400	0.0	39.375	2
>>END_MODULE