FastQCFastQC Report
Sat 14 Jan 2017
SRR2933389.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933389.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426478
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207464.86449476878057No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCG60541.4195339501685902No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC34870.8176271695140194No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC32780.7686211246535578No Hit
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC18140.4253443319467827TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCCTTTT13910.32615984880814486No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT13510.3167807014664297TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCC13160.3085739475424289No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTC9090.21314112334047713No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTA8110.19016221235327496No Hit
CTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT7120.16694882268252992TruSeq Adapter, Index 20 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCT6510.1526456229864143No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC5550.13013566936629792No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCAGCCTTTTCGTATGCCG5320.12474265964481168No Hit
TCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC4810.11278424678412485TruSeq Adapter, Index 23 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACGG351.2095188E-745.02
CGTTTTT121000.044.4421461
ACCGGTG1100.040.90909217
TACGGCT6050.040.537197
ACGGCTG6050.040.537198
CGATGAA1350.040.00000419
GAATGAT26050.039.9904021
AATGATA24250.039.8969042
GTTTTTT136850.039.8374142
ATGATAC25000.039.6000023
CGGGAAC1150.039.1304366
ATACGGC17150.039.096216
TGATACC8300.039.0361444
ACGGCGA10600.038.844348
TGATACG17500.038.828574
GATACCT8350.038.8023955
CACCGGT1450.038.79310216
CGACCAC6800.038.71323412
GATACGG17500.038.6999975
TACGGCG11350.038.259917
Produced by FastQC (version 0.11.5)