##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933389.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426478
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.656050253471456	31.0	28.0	31.0	16.0	33.0
2	29.06729303738997	31.0	30.0	33.0	16.0	34.0
3	29.237121258306406	31.0	30.0	33.0	16.0	34.0
4	32.17220583476756	35.0	31.0	37.0	19.0	37.0
5	33.62097458720028	35.0	33.0	37.0	28.0	37.0
6	32.88003367113895	35.0	33.0	37.0	23.0	37.0
7	32.02107963365051	35.0	31.0	35.0	25.0	37.0
8	33.05530883187409	35.0	32.0	35.0	28.0	37.0
9	32.798360056087304	35.0	31.0	37.0	25.0	39.0
10	31.717605597475135	35.0	28.0	39.0	15.0	39.0
11	32.37978981330807	35.0	30.0	39.0	17.0	39.0
12	33.21494426441692	35.0	31.0	39.0	23.0	39.0
13	33.36066807666515	35.0	32.0	39.0	25.0	39.0
14	33.36679734945296	36.0	31.0	39.0	18.0	41.0
15	34.0663551226558	36.0	32.0	40.0	25.0	41.0
16	34.16485258325166	37.0	32.0	40.0	25.0	41.0
17	32.100750800744706	35.0	27.0	39.0	17.0	41.0
18	32.92568901561159	36.0	30.0	39.0	21.0	39.0
19	32.771807689962905	36.0	30.0	37.0	19.0	39.0
20	32.5263671279644	34.0	30.0	37.0	24.0	39.0
21	32.74631282270129	35.0	31.0	37.0	24.0	39.0
22	33.05963496358546	35.0	31.0	38.0	25.0	39.0
23	34.11439277055323	35.0	33.0	38.0	27.0	40.0
24	32.25478688232453	35.0	30.0	37.0	21.0	39.0
25	31.287606863660024	34.0	28.0	37.0	18.0	39.0
26	30.74983234774127	33.0	25.0	37.0	18.0	39.0
27	32.40397628951552	34.0	30.0	38.0	22.0	39.0
28	32.45551470415825	35.0	31.0	38.0	21.0	39.0
29	32.66708013074531	35.0	31.0	38.0	21.0	40.0
30	31.546597948780477	35.0	29.0	38.0	18.0	40.0
31	31.831034660639002	35.0	30.0	38.0	20.0	40.0
32	31.690797649585676	35.0	30.0	38.0	18.0	40.0
33	31.599055519862688	35.0	30.0	38.0	15.0	40.0
34	30.71446827268933	35.0	28.0	38.0	10.0	40.0
35	30.109025553486934	35.0	26.0	38.0	8.0	40.0
36	29.533652380662073	35.0	24.0	38.0	7.0	40.0
37	29.640405835705476	35.0	24.0	38.0	7.0	40.0
38	29.602443736839884	35.0	23.0	38.0	7.0	40.0
39	29.21158418488175	34.0	23.0	38.0	7.0	40.0
40	29.118282771913204	34.0	23.0	38.0	7.0	40.0
41	28.659637777329664	34.0	21.0	38.0	7.0	40.0
42	29.075907784223336	34.0	22.0	38.0	7.0	40.0
43	29.07915296920357	34.0	22.0	38.0	7.0	40.0
44	28.96171901012479	34.0	22.0	38.0	7.0	40.0
45	29.18523581521204	35.0	23.0	38.0	7.0	40.0
46	28.995263530592435	34.0	22.0	38.0	7.0	40.0
47	29.106727662388213	35.0	22.0	38.0	7.0	40.0
48	28.760388578074366	34.0	22.0	38.0	7.0	40.0
49	28.564090058572774	34.0	21.0	37.0	7.0	40.0
50	28.761429663429297	34.0	22.0	38.0	7.0	40.0
51	27.33176388934482	32.0	20.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	18.0
10	31.0
11	30.0
12	41.0
13	69.0
14	111.0
15	209.0
16	442.0
17	879.0
18	1573.0
19	2517.0
20	3793.0
21	5444.0
22	7736.0
23	10413.0
24	13928.0
25	18427.0
26	22310.0
27	23179.0
28	21792.0
29	22317.0
30	24606.0
31	28749.0
32	33606.0
33	38723.0
34	43348.0
35	45917.0
36	34686.0
37	17860.0
38	3660.0
39	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.4726199241227	2.587941230262757	37.288441607773436	38.6509972378411
2	40.25741069879337	9.391105754575852	35.43371522094926	14.917768325681513
3	13.54278532538607	9.4405807568034	62.0812796908633	14.93535422694723
4	12.32771678726687	3.1408419660568656	67.09162020080754	17.43982104586872
5	17.20135622470561	3.7441556188126937	61.95583359516787	17.09865456131383
6	14.593015348974625	9.192971266982118	63.84010429611844	12.373909087924817
7	54.83096431703394	1.4608021984721369	40.573722442892716	3.134511041601208
8	56.05142586487462	6.302552534949048	33.556713359188514	4.089308240987812
9	51.606647939635806	3.1560830804871527	34.41889147857568	10.818377501301358
10	25.964997022120716	18.522409127786194	41.751274391645055	13.761319458448032
11	21.25643057789616	18.657703328190433	46.040827428378485	14.045038665534918
12	16.893954670580897	16.676123973569563	50.328035678276485	16.10188567757305
13	16.851982986226723	17.283658242629162	53.16663462124658	12.697724149897532
14	15.22141822086954	18.682323589962436	49.55191123574956	16.544346953418465
15	13.130337321034144	17.765511937309782	53.21868888899311	15.885461852662974
16	14.657965944316	17.205576841009385	48.20928629378303	19.927170920891584
17	15.472075933576878	17.995770004548888	49.86939537326662	16.662758688607617
18	15.634569661272094	16.570374087291725	50.151707708252246	17.64334854318394
19	15.661300231195982	19.371221962211415	46.94825993368943	18.019217872903173
20	17.077317001111428	19.057723962314586	50.250188755340254	13.614770281233731
21	17.57019119391856	20.761680555620686	47.324129263408665	14.343998987052087
22	15.970812093472583	17.387297820755116	48.90897068547498	17.73291940029732
23	15.653327955955524	20.846092881696123	47.46434751616731	16.036231646181047
24	15.701396086081814	18.916567794821773	47.679364468976125	17.702671650120287
25	14.409653018444093	21.304733186705995	45.95313240073345	18.332481394116463
26	14.845314412466761	19.003090429049095	48.382800519604764	17.768794638879378
27	15.552267643348545	19.895516298613295	46.77849736680438	17.773718691233782
28	14.211987488217446	19.688237142361388	48.2812712496307	17.81850411979047
29	16.082658425522535	18.20961456393999	46.36347009693349	19.344256913603985
30	15.754857225929591	20.977869901847225	46.60216939678014	16.665103475443047
31	17.205811319692927	19.262189374363977	45.05038946909337	18.48160983684973
32	16.677999803037906	20.631075928887306	44.14905340955454	18.54187085852025
33	15.96002607402961	19.52128831967886	42.971501460802195	21.547184145489336
34	16.995952897922052	21.154432350555012	41.93182297797307	19.91779177354987
35	14.988111930744378	24.269716140105704	40.265382974033834	20.476788955116092
36	18.86029291077148	22.22365514751054	38.31123762538748	20.604814316330504
37	16.852920900960893	24.820506567747927	38.68781039115734	19.63876214013384
38	19.259844587528548	23.76722832127331	36.77985734316893	20.193069748029206
39	18.073382448801578	22.592021159356403	38.86812449880181	20.466471893040204
40	18.099409582674838	23.055819995404217	39.44048696532998	19.404283456590964
41	17.60911465538668	23.623961845628614	39.753281529176185	19.013641969808525
42	17.73784345265172	23.29592616735213	39.74741956208761	19.218810817908544
43	18.696626789658552	22.280164510244376	37.804060232884225	21.219148467212847
44	18.996056068542806	23.757614695248055	36.212887886362246	21.033441349846886
45	18.660282593709407	22.579828267812175	36.76414727137156	21.99574186710686
46	19.264299682515862	23.51352238567992	36.58359868504355	20.638579246760678
47	16.377163652052392	22.812196643203166	40.38262231580527	20.42801738893917
48	18.09612688110524	23.421137784364024	37.11281707379982	21.369918260730916
49	17.63045221558908	21.856696007765933	39.41422535277318	21.098626423871806
50	17.83163492606887	21.272140649693537	38.06808323055351	22.828141193684083
51	17.853206964954815	21.716946712374376	36.811043008080134	23.618803314590668
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	60.0
1	315.0
2	570.0
3	15909.0
4	31248.0
5	21910.5
6	12573.0
7	11890.0
8	11207.0
9	11037.0
10	10867.0
11	10616.0
12	10365.0
13	9763.5
14	9162.0
15	8739.5
16	8317.0
17	7663.5
18	7010.0
19	6563.0
20	6116.0
21	5884.0
22	5652.0
23	5492.5
24	5333.0
25	5150.5
26	5112.0
27	5256.0
28	5524.0
29	5792.0
30	6192.0
31	6592.0
32	7067.0
33	7542.0
34	8602.5
35	9663.0
36	10505.5
37	11348.0
38	12241.5
39	13135.0
40	14038.5
41	14942.0
42	16263.0
43	17584.0
44	18608.0
45	19632.0
46	29759.0
47	39886.0
48	35457.5
49	31029.0
50	29194.5
51	27360.0
52	23540.0
53	19720.0
54	18045.0
55	16370.0
56	14964.5
57	13559.0
58	12361.5
59	11164.0
60	9882.0
61	8600.0
62	7795.5
63	6991.0
64	6057.5
65	5124.0
66	4408.5
67	3693.0
68	3215.0
69	2737.0
70	2335.5
71	1934.0
72	1632.0
73	1330.0
74	1105.0
75	755.0
76	630.0
77	474.5
78	319.0
79	215.0
80	111.0
81	88.0
82	65.0
83	41.5
84	18.0
85	15.0
86	12.0
87	9.5
88	7.0
89	5.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	426478.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.04102966204126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.059389994157	43.80532899061209
2	7.894715107681857	8.532770666152011
3	3.670899813925937	5.951376171922599
4	2.172879640111113	4.696986123331608
5	1.4547740833369156	3.930874469458957
6	1.0135034307448056	3.2862461378076366
7	0.7366721238756151	2.7867364068292733
8	0.509281205735401	2.201766459637356
9	0.3468155282456886	1.686804142426359
>10	1.0667869603626268	8.52752344096851
>50	0.04112045475929748	1.5244713058061587
>100	0.026971481078482948	2.6494259935241877
>500	0.002652932565096683	0.9964020917204062
>1k	0.002652932565096683	3.0195523340697297
>5k	4.4215542751611383E-4	1.4465751230876112
>10k+	4.4215542751611383E-4	4.957160142645455
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20746	4.86449476878057	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCG	6054	1.4195339501685902	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC	3487	0.8176271695140194	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC	3278	0.7686211246535578	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	1814	0.4253443319467827	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCCTTTT	1391	0.32615984880814486	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT	1351	0.3167807014664297	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCC	1316	0.3085739475424289	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTC	909	0.21314112334047713	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTA	811	0.19016221235327496	No Hit
CTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT	712	0.16694882268252992	TruSeq Adapter, Index 20 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCT	651	0.1526456229864143	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC	555	0.13013566936629792	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCAGCCTTTTCGTATGCCG	532	0.12474265964481168	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	481	0.11278424678412485	TruSeq Adapter, Index 23 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2077481136189909	0.0
2	0.0	0.0	0.0	0.983403598778835	0.0
3	0.0	0.0	0.0	1.2031101252585128	0.0
4	0.0	0.0	0.0	1.9480489028742396	0.0
5	0.0	0.0	0.0	3.264881189651049	0.0
6	0.0	0.0	0.0	3.660212250104343	0.0
7	0.0	0.0	0.0	4.138548764531817	0.0
8	0.0	0.0	0.0	4.742565853338273	0.0
9	0.0	0.0	0.0	4.982672025286181	0.0
10	0.0	0.0	0.0	7.05687045990649	0.0
11	0.0	0.0	0.0	7.918345143243028	0.0
12	0.0	0.0	0.0	9.47317329381586	0.0
13	0.0	0.0	0.0	9.754782192750858	0.0
14	0.0	0.0	0.0	9.927124025154873	0.0
15	0.0	0.0	0.0	10.395612434873545	0.0
16	0.0	0.0	0.0	10.732089345757577	0.0
17	0.0	0.0	0.0	11.103972537856583	0.0
18	0.0	0.0	0.0	11.452407861601301	0.0
19	0.0	0.0	0.0	12.11762388681245	0.0
20	0.0	0.0	0.0	12.424790962253622	0.0
21	0.0	0.0	0.0	12.756109342099709	0.0
22	0.0	0.0	0.0	13.178639929843978	0.0
23	0.0	0.0	0.0	13.510427267057151	0.0
24	0.0	0.0	0.0	13.773981307359348	0.0
25	0.0	0.0	0.0	13.99814292882634	0.0
26	0.0	0.0	0.0	14.201201468774475	0.0
27	0.0	0.0	0.0	14.441542119405925	0.0
28	0.0	0.0	0.0	14.644131701986973	0.0
29	0.0	0.0	0.0	14.894085978643682	0.0
30	0.0	0.0	0.0	15.142398904515591	0.0
31	0.0	0.0	0.0	15.37851893884327	0.0
32	2.344786835428791E-4	0.0	0.0	15.622142291044321	0.0
33	2.344786835428791E-4	0.0	0.0	15.862951899042859	0.0
34	2.344786835428791E-4	0.0	0.0	16.095085795750308	0.0
35	2.344786835428791E-4	0.0	0.0	16.345040072407016	0.0
36	2.344786835428791E-4	0.0	0.0	16.580222192000527	0.0
37	2.344786835428791E-4	0.0	0.0	16.810714737923174	0.0
38	2.344786835428791E-4	0.0	0.0	17.052462260655883	0.0
39	2.344786835428791E-4	0.0	0.0	17.337822818527567	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACGG	35	1.2095188E-7	45.0	2
CGTTTTT	12100	0.0	44.442146	1
ACCGGTG	110	0.0	40.909092	17
TACGGCT	605	0.0	40.53719	7
ACGGCTG	605	0.0	40.53719	8
CGATGAA	135	0.0	40.000004	19
GAATGAT	2605	0.0	39.990402	1
AATGATA	2425	0.0	39.896904	2
GTTTTTT	13685	0.0	39.837414	2
ATGATAC	2500	0.0	39.600002	3
CGGGAAC	115	0.0	39.130436	6
ATACGGC	1715	0.0	39.09621	6
TGATACC	830	0.0	39.036144	4
ACGGCGA	1060	0.0	38.84434	8
TGATACG	1750	0.0	38.82857	4
GATACCT	835	0.0	38.802395	5
CACCGGT	145	0.0	38.793102	16
CGACCAC	680	0.0	38.713234	12
GATACGG	1750	0.0	38.699997	5
TACGGCG	1135	0.0	38.25991	7
>>END_MODULE