SRR2933385.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933385.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	222608
Sequences flagged as poor quality	0
Sequence length	51
%GC	35

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16724	7.5127578523682885	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCG	7104	3.1912599726874142	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGC	5033	2.2609250341407314	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC	3558	1.5983253072665853	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTACAGGT	1558	0.6998849996406239	No Hit
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	1467	0.6590059656436427	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	1177	0.5287321210378783	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTA	1030	0.46269675842737007	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTC	965	0.43349744842952637	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	655	0.29423920074750237	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTCTACAGGTCGTATGCCG	615	0.2762703945949831	No Hit
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	411	0.18462948321713504	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGT	390	0.17519585998706247	No Hit
GAATCTTTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC	307	0.13791058722058505	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	303	0.13611370660533312	No Hit
GAATGACTCTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	301	0.1352152662977072	No Hit
TCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	286	0.12847696399051245	Illumina Single End Adapter 2 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTT	264	0.11859412060662688	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC	263	0.11814490045281391	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTAT	256	0.11500035937612305	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATG	249	0.1118558182994322	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	249	0.1118558182994322	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCTCTACAGGTCGTATGC	246	0.11050815783799324	No Hit
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	238	0.1069143966074894	Illumina Single End Adapter 2 (95% over 21bp)
GAATCTGTCTCTTATCCACATCTGACGCTCTACAGGTCGTATGCCGTCTTC	237	0.10646517645367642	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCT	20	7.02102E-4	45.000004	30
CGTGGCG	20	7.02102E-4	45.000004	27
CCCCTAC	25	3.8806415E-5	45.000004	41
ACGCCGG	30	2.1575925E-6	45.000004	27
TTGTGAC	25	3.8806415E-5	45.000004	43
TCGGGCA	25	3.8806415E-5	45.000004	5
TCCTCGG	20	7.02102E-4	45.000004	2
GGCTTGT	20	7.02102E-4	45.000004	21
AGACGTG	20	7.02102E-4	45.000004	24
ATGACTC	40	6.7720975E-9	45.000004	3
GGTGATA	20	7.02102E-4	45.000004	8
GGCGCGA	20	7.02102E-4	45.000004	8
CGGTAGT	30	2.1575925E-6	45.000004	12
GGATTAC	30	2.1575925E-6	45.000004	8
CTAATGC	25	3.8806415E-5	45.000004	23
CAGACGT	20	7.02102E-4	45.000004	23
GTCTTAT	55	1.8189894E-12	45.0	23
CGTTTTT	7405	0.0	44.544228	1
TACGGCT	630	0.0	43.928574	7
ACGGCTG	640	0.0	43.24219	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content