FastQCFastQC Report
Sat 14 Jan 2017
SRR2933384.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933384.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924934
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329493.5623082295601636No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC218972.3674121613001575No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG207612.2445925871467582No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC201552.1790743988219696No Hit
GAACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT59600.6443703010160725No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTC48410.5233886958420817No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGATCAGT47170.5099823338746332No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTA40910.4423018291034819No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC39570.4278143089128521No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCT35160.3801352312705555No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGT16750.1810940023828727No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTT14550.15730852147288346No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11410.12336015326498972No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10490.11341349761172148No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA10370.1121161077439039No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTAT10210.11038625458681375No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATG10110.10930509636363243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG406.8121153E-945.06
CGCCGAT253.890213E-545.016
CGTTTTT298350.044.4117661
TGATACC24450.042.239264
GATACCT24350.042.043125
ACGGCTG24000.041.906258
TACGGCT24000.041.81257
CCGATGA6800.041.36029418
CGGCTGT24800.041.280249
GCGACTG5150.041.0679611
CACCGGT2150.040.81395316
GAATCTG27750.040.5405431
ATACCTG25450.040.2259336
AATCTGT28100.040.0355872
ATGATAC84950.040.0206033
GAATGAT88900.039.9128231
CCGGTGA1300.039.8076918
AATGATA83400.039.766192
CGCTTTT8550.039.7368431
GGCGACT5550.039.7297310
Produced by FastQC (version 0.11.5)