##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933384.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	924934
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.543320928844654	31.0	28.0	33.0	25.0	34.0
2	28.846526346744742	31.0	28.0	33.0	16.0	34.0
3	28.811939014026947	31.0	28.0	34.0	16.0	34.0
4	33.25383973342963	35.0	32.0	37.0	28.0	37.0
5	34.329783530500556	35.0	33.0	37.0	30.0	37.0
6	33.836950528362024	35.0	33.0	37.0	28.0	37.0
7	33.82566431767023	35.0	33.0	37.0	29.0	37.0
8	33.64109979739095	35.0	33.0	37.0	28.0	37.0
9	34.916237266659024	37.0	34.0	39.0	28.0	39.0
10	34.96460504208949	37.0	34.0	39.0	28.0	39.0
11	35.33227343788854	37.0	34.0	39.0	30.0	39.0
12	35.45443891131691	37.0	34.0	39.0	30.0	39.0
13	35.656473867324586	37.0	35.0	39.0	30.0	39.0
14	36.41588913371116	38.0	35.0	40.0	30.0	41.0
15	36.707184512624686	39.0	35.0	41.0	30.0	41.0
16	36.63964563958077	38.0	34.0	41.0	31.0	41.0
17	35.855418873130404	38.0	34.0	40.0	27.0	41.0
18	35.55189775702915	38.0	34.0	39.0	28.0	40.0
19	34.81028916657837	37.0	34.0	38.0	27.0	40.0
20	33.998985873586655	35.0	33.0	38.0	27.0	40.0
21	34.35298302365358	35.0	33.0	38.0	29.0	40.0
22	34.74247459818755	35.0	33.0	39.0	29.0	40.0
23	35.072170554872024	35.0	33.0	39.0	30.0	40.0
24	34.48219008058953	35.0	33.0	38.0	27.0	40.0
25	33.51762828482897	35.0	33.0	38.0	24.0	40.0
26	33.448147651616225	35.0	32.0	38.0	24.0	40.0
27	34.27070580171126	35.0	33.0	38.0	26.0	40.0
28	34.058522013462586	35.0	33.0	39.0	25.0	40.0
29	33.95675367107275	35.0	33.0	39.0	24.0	40.0
30	33.19653834760102	35.0	33.0	39.0	21.0	40.0
31	33.06582523726017	35.0	33.0	38.0	21.0	40.0
32	32.28484951358691	35.0	31.0	38.0	16.0	40.0
33	31.45877003115898	35.0	30.0	39.0	12.0	40.0
34	30.753005079281333	35.0	25.0	39.0	8.0	40.0
35	30.11913606808702	35.0	23.0	39.0	7.0	41.0
36	29.491555073118732	35.0	21.0	39.0	7.0	40.0
37	29.250667615202815	35.0	18.0	39.0	7.0	40.0
38	29.0980448334692	35.0	18.0	39.0	7.0	40.0
39	28.68151781640636	35.0	18.0	39.0	7.0	40.0
40	28.504837101890512	35.0	18.0	39.0	7.0	40.0
41	28.166155639213176	34.0	17.0	39.0	7.0	40.0
42	28.18380446604839	35.0	15.0	39.0	7.0	40.0
43	28.104033368867402	35.0	15.0	39.0	7.0	40.0
44	28.12049184049889	35.0	15.0	39.0	7.0	40.0
45	28.24753982446315	35.0	16.0	39.0	7.0	40.0
46	28.01756665881025	35.0	15.0	39.0	7.0	40.0
47	27.926982898239224	34.0	15.0	39.0	7.0	40.0
48	27.77986861765272	34.0	15.0	38.0	7.0	40.0
49	27.946206972605612	34.0	15.0	39.0	7.0	40.0
50	27.92894844388897	34.0	15.0	39.0	7.0	40.0
51	26.941148233279346	33.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	17.0
9	49.0
10	51.0
11	63.0
12	78.0
13	109.0
14	158.0
15	251.0
16	502.0
17	1092.0
18	2193.0
19	3882.0
20	6300.0
21	9245.0
22	13309.0
23	19062.0
24	28286.0
25	40944.0
26	53022.0
27	56441.0
28	51293.0
29	44126.0
30	41664.0
31	43295.0
32	47601.0
33	55018.0
34	63988.0
35	77119.0
36	86754.0
37	92901.0
38	74216.0
39	11904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.16204831912331	2.0469568639492115	29.551297714215284	44.23969710271219
2	42.632771635597784	15.226491836174258	30.206263365818536	11.934473162409425
3	10.541076444373328	15.349203294505337	61.97090819453064	12.138812066590697
4	9.508894688702114	2.4942320208793274	74.33254697091901	13.664326319499553
5	19.098984359965144	2.9096130102255944	62.64760512641983	15.34379750338943
6	12.151677849446555	13.050660912021831	65.40823453349104	9.389426705040576
7	47.35851422912338	0.9743397907310143	49.47023247064115	2.196913509504462
8	46.531211956745025	9.816916666486474	40.678902494664484	2.9729688821040203
9	43.77025820220686	2.7153288775199096	39.92436217070623	13.590050749566995
10	26.299065663063526	18.608679105752408	44.95780239454923	10.13445283663483
11	19.38873476377774	14.647423491838337	53.56652474663057	12.397316997753354
12	14.378647557555457	13.31122004380853	55.68721660140075	16.62291579723526
13	14.896630462281632	13.84174438392361	60.67049108368814	10.591134070106625
14	13.20483407464749	19.47328133683052	53.01264738889478	14.309237199627217
15	9.94687188491287	14.921388985592484	61.66407549079177	13.46766363870287
16	11.96723225657182	16.166667027052743	52.80636239991178	19.059738316463662
17	12.475700968934	16.62399695545844	56.88762657659898	14.012675499008578
18	12.198600116332624	15.352663000819518	55.60483234479433	16.843904538053525
19	12.115783396436935	17.088786875604097	53.58187719339974	17.213552534559224
20	14.25334131948874	16.882177539154146	57.60908345892788	11.255397682429233
21	14.617042945766942	20.205333569746596	53.52035929050073	11.657264193985734
22	12.8382133211667	13.513180399898802	56.36142686937663	17.287179409557872
23	13.264189661100142	19.640320282312036	54.94143365905026	12.154056397537554
24	14.322859793239301	15.966004060830286	53.156008969288614	16.555127176641793
25	11.677806200226179	23.015804370906466	51.351555894798985	13.954833534068376
26	13.07282465559705	15.254493834154653	54.90997195475569	16.76270955549261
27	15.232870669691026	17.15571056961902	52.09020319287646	15.521215567813487
28	12.030696244272564	17.21841774656354	55.828848328637505	14.922037680526396
29	17.058838792821973	14.70104893970813	52.970049754901424	15.270062512568463
30	13.279217760402364	18.30508987668309	56.362291795955166	12.053400566959374
31	18.155457578594795	16.566046874695925	49.03787729719093	16.24061824951834
32	16.370789699589373	22.778706372562798	48.30831172818817	12.542192199659652
33	14.61585367172144	18.000527605212913	48.10126992844895	19.282348794616695
34	19.607993651438914	21.042366266133584	43.202325787569706	16.147314294857797
35	14.783757543781503	24.5817539413623	44.269537069672	16.364951445184197
36	23.24479368257627	21.062583924907074	39.813219105363196	15.879403287153462
37	15.78858599640623	25.217799324059882	43.81134221468775	15.182272464846141
38	20.009860162995412	23.375397595936576	40.31195739371674	16.30278484735127
39	19.01411343944541	25.26558651752449	38.0442280205939	17.676072022436195
40	16.709516570912086	25.595556007239434	42.65180002032577	15.043127401522705
41	19.841740059290718	25.07368093290981	36.66953533981884	18.415043667980633
42	16.5790207733741	26.645360641948507	40.7034447863307	16.072173798346693
43	22.011948960682602	22.45727803281099	37.43650898334367	18.094264023162733
44	18.714416379979546	23.561356810323765	38.31581496625705	19.40841184343964
45	17.486761217557145	22.864225988016443	38.267919656970115	21.381093137456293
46	21.5904053694642	24.87604520971226	36.76846131723993	16.765088103583604
47	15.56381320180683	21.945025266667674	45.420862461537794	17.070299069987698
48	18.118698199006634	23.784183520121434	37.10578268287251	20.991335597999424
49	16.175640640305147	22.772651886512985	42.484220495732664	18.567486977449203
50	18.685657571242924	20.90105888636378	40.6014915658847	19.811791976508594
51	17.427838094393763	21.203891304676876	38.720600605016145	22.647669995913223
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	98.0
1	1735.5
2	3373.0
3	39483.0
4	75593.0
5	57243.0
6	38893.0
7	37266.0
8	35639.0
9	34297.0
10	32955.0
11	31184.0
12	29413.0
13	27574.5
14	25736.0
15	23582.0
16	21428.0
17	19635.0
18	17842.0
19	16236.0
20	14630.0
21	13368.5
22	12107.0
23	11638.0
24	11169.0
25	11567.0
26	12019.5
27	12074.0
28	12929.0
29	13784.0
30	15162.0
31	16540.0
32	17632.0
33	18724.0
34	20610.0
35	22496.0
36	23810.0
37	25124.0
38	26277.0
39	27430.0
40	28068.0
41	28706.0
42	30201.0
43	31696.0
44	33683.5
45	35671.0
46	80284.0
47	124897.0
48	87866.5
49	50836.0
50	45768.5
51	40701.0
52	35359.5
53	30018.0
54	27632.5
55	25247.0
56	23637.0
57	22027.0
58	20279.0
59	18531.0
60	16787.5
61	15044.0
62	13119.0
63	11194.0
64	9612.0
65	8030.0
66	6721.5
67	5413.0
68	4391.5
69	3370.0
70	2872.0
71	2374.0
72	2028.5
73	1683.0
74	1414.5
75	905.5
76	665.0
77	488.5
78	312.0
79	264.5
80	217.0
81	152.0
82	87.0
83	61.5
84	36.0
85	22.0
86	8.0
87	7.0
88	6.0
89	6.0
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	924934.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.72673471978072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.7146341472747	38.279968678159264
2	5.354131299212353	4.896538831479163
3	2.381761472390706	3.2673052504141245
4	1.5619508810995824	2.8569165434147346
5	1.2561086699423676	2.871887396483562
6	1.0263614586068603	2.8159294886593864
7	0.8532741687933214	2.731220909175752
8	0.6822580252322243	2.4957945384188305
9	0.573782624853592	2.3613485286149696
>10	2.487679160862716	16.817310990232254
>50	0.05640945829784128	1.7936183539700465
>100	0.04522230577262869	3.836875360200023
>500	0.002380121355965635	0.6925182366285327
>1k	0.002856145627158762	3.2118349333874945
>5k	2.3801213559656348E-4	0.6486576599569472
>10k+	9.520485423862539E-4	10.422274300804895
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32949	3.5623082295601636	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC	21897	2.3674121613001575	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG	20761	2.2445925871467582	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC	20155	2.1790743988219696	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT	5960	0.6443703010160725	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTC	4841	0.5233886958420817	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGATCAGT	4717	0.5099823338746332	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTA	4091	0.4423018291034819	No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	3957	0.4278143089128521	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCT	3516	0.3801352312705555	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGT	1675	0.1810940023828727	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTT	1455	0.15730852147288346	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1141	0.12336015326498972	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1049	0.11341349761172148	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	1037	0.1121161077439039	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTAT	1021	0.11038625458681375	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATG	1011	0.10930509636363243	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0675723889488331	0.0
2	0.0	0.0	0.0	0.5602561912525651	0.0
3	0.0	0.0	0.0	0.639072625722484	0.0
4	0.0	0.0	0.0	1.4047488793795018	0.0
5	0.0	0.0	0.0	4.153485545995714	0.0
6	0.0	0.0	0.0	4.39058354433938	0.0
7	0.0	0.0	0.0	4.874293733390707	0.0
8	0.0	0.0	0.0	5.53715184002318	0.0
9	0.0	0.0	0.0	5.621482181431324	0.0
10	0.0	0.0	0.0	8.30048414265234	0.0
11	0.0	0.0	0.0	8.460495559683178	0.0
12	0.0	0.0	0.0	11.128037243738472	0.0
13	0.0	0.0	0.0	11.332268032097426	0.0
14	0.0	0.0	0.0	11.489792785214945	0.0
15	0.0	0.0	0.0	12.068861129550866	0.0
16	0.0	0.0	0.0	12.342069812548788	0.0
17	0.0	0.0	0.0	12.468673440483322	0.0
18	0.0	0.0	0.0	12.575059409644364	0.0
19	0.0	0.0	0.0	13.28754267872086	0.0
20	0.0	0.0	0.0	13.439445409077837	0.0
21	0.0	0.0	0.0	13.54864238961915	0.0
22	0.0	0.0	0.0	13.79309226388045	0.0
23	0.0	0.0	0.0	13.911587205141124	0.0
24	0.0	0.0	0.0	14.02067606986012	0.0
25	0.0	0.0	0.0	14.10868234922708	0.0
26	0.0	0.0	0.0	14.222528310128075	0.0
27	0.0	0.0	0.0	14.373782345551142	0.0
28	0.0	0.0	0.0	14.460058771761013	0.0
29	0.0	0.0	0.0	14.563201266252511	0.0
30	0.0	0.0	0.0	14.68839938849691	0.0
31	0.0	0.0	0.0	14.796947674104315	0.0
32	0.0	0.0	0.0	14.92171333305944	0.0
33	0.0	0.0	0.0	15.035559293960434	0.0
34	0.0	0.0	0.0	15.200544038817904	0.0
35	0.0	0.0	0.0	15.34152707112075	0.0
36	0.0	0.0	0.0	15.47526634332828	0.0
37	0.0	1.0811582231813297E-4	0.0	15.620357776879215	0.0
38	0.0	1.0811582231813297E-4	0.0	15.765881673719422	0.0
39	0.0	1.0811582231813297E-4	0.0	15.930109607820667	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGACG	40	6.8121153E-9	45.0	6
CGCCGAT	25	3.890213E-5	45.0	16
CGTTTTT	29835	0.0	44.411766	1
TGATACC	2445	0.0	42.23926	4
GATACCT	2435	0.0	42.04312	5
ACGGCTG	2400	0.0	41.90625	8
TACGGCT	2400	0.0	41.8125	7
CCGATGA	680	0.0	41.360294	18
CGGCTGT	2480	0.0	41.28024	9
GCGACTG	515	0.0	41.06796	11
CACCGGT	215	0.0	40.813953	16
GAATCTG	2775	0.0	40.540543	1
ATACCTG	2545	0.0	40.225933	6
AATCTGT	2810	0.0	40.035587	2
ATGATAC	8495	0.0	40.020603	3
GAATGAT	8890	0.0	39.912823	1
CCGGTGA	130	0.0	39.80769	18
AATGATA	8340	0.0	39.76619	2
CGCTTTT	855	0.0	39.736843	1
GGCGACT	555	0.0	39.72973	10
>>END_MODULE