FastQCFastQC Report
Sat 14 Jan 2017
SRR2933383.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933383.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553233
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417307.542933989837916No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG98791.7856852356963522No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC88221.594626495527201No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC67131.213412793524609No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGATCAGT24710.44664725350801565No Hit
GAACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT24600.444658941169453No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC21010.3797676566654556No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTC15860.2866784880872978No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTA14620.2642647853616831No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCT9200.16629521377068973No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGTGATCAGTCGTATGCCG7840.1417124430393704No Hit
GAATCTTTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC7740.13990488636794984No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7170.12960181334085277No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCC6200.11206851362807353No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGT6040.10917642295380064No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA6030.1089956672866586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC453.8380676E-1045.026
ATTCGGC351.2103919E-745.028
TTATACG351.2103919E-745.026
CTACGGG453.8380676E-1045.03
TATACGG302.1634532E-644.99999627
CGTTTTT201150.044.5749471
ACCGGTG1350.043.33333217
CGACCAC8650.042.65896212
TGATACC14300.042.4825134
GCGACCA8700.042.41379511
GATACCT14500.042.0517235
GAATGAT42850.041.3243871
GCGAGAC603.6379788E-1241.24999621
ATGATAC40750.041.1901863
CCACCGG1750.041.1428615
CACCGGT1700.041.0294116
TACGGCT11150.040.9641237
CGACCAA556.002665E-1140.90909229
ACCACCG4900.040.86734814
AATGATA40900.040.819072
Produced by FastQC (version 0.11.5)