##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933383.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553233
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.674811517028086	31.0	28.0	31.0	16.0	33.0
2	28.852143671834472	31.0	30.0	33.0	16.0	34.0
3	28.983894670057644	31.0	30.0	33.0	16.0	34.0
4	32.22762741918866	35.0	32.0	37.0	19.0	37.0
5	33.80639441248082	35.0	33.0	37.0	30.0	37.0
6	32.880227679838335	35.0	33.0	37.0	18.0	37.0
7	32.55267129762686	35.0	32.0	36.0	26.0	37.0
8	33.00737121610605	35.0	32.0	37.0	27.0	37.0
9	33.33699363559296	35.0	32.0	38.0	25.0	39.0
10	32.36021170103736	35.0	28.0	39.0	15.0	39.0
11	33.278170318834924	37.0	31.0	39.0	17.0	39.0
12	33.95824544089019	37.0	31.0	39.0	26.0	39.0
13	34.17373150191691	37.0	32.0	39.0	27.0	39.0
14	34.277778079037226	37.0	32.0	40.0	23.0	41.0
15	34.801112731886924	37.0	32.0	40.0	26.0	41.0
16	34.963783794531416	37.0	32.0	40.0	27.0	41.0
17	33.00665723122084	36.0	30.0	40.0	19.0	41.0
18	33.53232905484669	36.0	31.0	39.0	24.0	39.0
19	33.251687806041936	37.0	32.0	37.0	24.0	39.0
20	32.634633147335755	35.0	31.0	36.0	25.0	39.0
21	32.97221062373358	35.0	32.0	37.0	25.0	39.0
22	32.91491288480622	35.0	31.0	37.0	25.0	39.0
23	33.96269203030188	35.0	33.0	38.0	27.0	40.0
24	32.28287900396397	35.0	31.0	37.0	21.0	39.0
25	31.477354026242107	35.0	30.0	37.0	18.0	39.0
26	30.351672803321566	33.0	25.0	37.0	18.0	39.0
27	32.379368186641074	34.0	30.0	37.0	22.0	39.0
28	32.493741335025206	35.0	31.0	37.0	21.0	39.0
29	32.45143547113061	35.0	31.0	38.0	21.0	40.0
30	31.557528202402967	35.0	30.0	37.0	18.0	40.0
31	31.889037711054836	35.0	30.0	37.0	20.0	40.0
32	31.654669913038447	35.0	30.0	37.0	18.0	40.0
33	31.281051564169164	35.0	30.0	38.0	15.0	40.0
34	30.157685821344714	35.0	25.0	38.0	9.0	40.0
35	29.51056968763613	35.0	23.0	38.0	7.0	40.0
36	28.873566833504146	34.0	21.0	38.0	7.0	40.0
37	28.75271359445297	35.0	21.0	38.0	7.0	40.0
38	28.804778456816567	35.0	21.0	38.0	7.0	40.0
39	28.34670021491849	34.0	20.0	38.0	7.0	40.0
40	28.101281015413036	33.0	18.0	38.0	7.0	40.0
41	27.751929114857575	33.0	18.0	37.0	7.0	40.0
42	27.962164223753824	33.0	18.0	38.0	7.0	40.0
43	28.026243915312357	34.0	18.0	38.0	7.0	40.0
44	27.942454625808654	34.0	18.0	38.0	7.0	40.0
45	28.164849168433552	34.0	18.0	38.0	7.0	40.0
46	27.880426511072187	34.0	18.0	38.0	7.0	40.0
47	27.89294926369179	34.0	18.0	38.0	7.0	40.0
48	27.674005346752633	33.0	18.0	37.0	7.0	40.0
49	27.418521671700713	33.0	18.0	37.0	7.0	40.0
50	27.63549715942469	33.0	18.0	37.0	7.0	40.0
51	26.23882523276811	31.0	15.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	8.0
10	25.0
11	29.0
12	32.0
13	63.0
14	98.0
15	250.0
16	573.0
17	1045.0
18	1876.0
19	3157.0
20	4793.0
21	7048.0
22	9807.0
23	13621.0
24	18982.0
25	25785.0
26	32534.0
27	34303.0
28	32110.0
29	30802.0
30	32317.0
31	36521.0
32	40992.0
33	46835.0
34	52619.0
35	57606.0
36	41630.0
37	22599.0
38	5071.0
39	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.320673206406703	1.9951810539139927	31.35568557913212	45.32846016054719
2	45.52530308206488	11.987354333526742	30.861138073831462	11.626204510576917
3	10.586317157508681	11.869863149884406	65.87387231058162	11.669947382025295
4	9.83220451419203	2.4317059900620537	74.10729294890218	13.628796546843736
5	17.339168126268678	2.7905059893390307	65.23634707257159	14.633978811820697
6	12.283432116305427	10.301626981759945	67.14259633825169	10.272344563682932
7	46.84771154287615	0.9637892172014323	49.84229068041856	2.3462085595038618
8	47.60905441287848	7.833950613936623	41.28116001756945	3.275834955615446
9	44.07365431924705	2.5564274003900707	41.246635685145314	12.123282595217566
10	24.835647909651087	18.87215693930044	45.91139718708031	10.380797963968165
11	17.960786865570203	14.86552682142967	55.200973188511895	11.972713124488235
12	14.189139114984103	13.55269841097693	56.75764099393926	15.500521480099705
13	14.260718359172357	14.061525613981813	61.05799907091587	10.619756955929962
14	12.876672215865648	17.211373869599246	55.10173832725091	14.810215587284201
15	9.753756554652378	14.99765921411051	61.70257377994444	13.546010451292675
16	11.702483402110865	14.874203093452486	54.413420746773966	19.009892757662687
17	12.201549799090076	15.609697903053505	57.86766154585862	14.321090751997803
18	11.965121386468269	14.226736293749651	56.97725912951685	16.830883190265222
19	12.103941738833367	16.365798858708718	54.948819032848725	16.581440369609187
20	13.85456037510416	16.220290546659363	58.509344164212905	11.415804914023568
21	14.592405008378027	18.790816889086514	54.383957573029804	12.232820529505652
22	12.737851863500552	13.874624254156927	56.93911968374989	16.448404198592637
23	12.800754835665984	19.090690540875183	55.17548663944486	12.933067984013968
24	13.317354532357975	15.423338810230048	54.844523012907764	16.41478364450421
25	11.588788087478513	20.190046508433156	53.03443576214724	15.186729641941099
26	12.779606422610366	15.619277953412034	55.68521762078545	15.915898003192144
27	13.504075136515716	16.919272711497687	54.05841661650697	15.518235535479628
28	11.844195845150235	16.60801145267907	57.113910413876255	14.433882288294445
29	15.563243696597997	14.51088420249696	54.65310276140433	15.272769339500716
30	12.923487933655439	17.209024045926398	57.37853671057221	12.488951309845941
31	16.33073225928316	16.456176692279744	51.49277067709266	15.720320371344442
32	15.054235737925973	21.25523965490128	51.04811173592319	12.642412871249546
33	13.633496194189428	17.446898503885343	50.474212492747185	18.44539280917805
34	18.294281071447294	20.37965920326517	45.62688776699871	15.69917195828882
35	13.948011055016602	23.13383330350865	46.28863426440578	16.629521377068976
36	21.219630788474294	20.12967411560771	42.7268438433716	15.923851252546395
37	15.485338004059773	24.02532025385326	45.51084262869352	14.978499113393454
38	18.777802481052287	22.499200156172897	43.3887710964458	15.334226266329015
39	17.539083894127792	23.95663310033928	41.28748646591942	17.216796539613508
40	16.05977951423722	24.02730856619182	44.764863990398254	15.1480479291727
41	18.941928626817273	23.267050230192343	39.98984153150661	17.801179611483768
42	16.376282687402956	24.831129017972536	42.83746631166254	15.95512198296197
43	20.913249932668514	21.25939703524555	40.559221883004085	17.268131149081853
44	17.870770543333457	22.189204187024274	41.479629740091426	18.46039552955084
45	17.123345859701068	21.696644994062176	40.953449993040905	20.22655915319585
46	19.685195930105397	23.739907055435953	39.805832262356006	16.76906475210264
47	15.192513823289644	20.93132549938272	47.138908922641996	16.73725175468564
48	17.317838957545916	22.4502153703774	40.18523840768718	20.046707264389507
49	15.678385056567487	21.586745548439808	44.10619033933261	18.628679055660093
50	18.000553112341457	19.83919975851043	42.51554046848254	19.644706660665577
51	16.753519764728424	20.03098152134815	41.31911870766928	21.896380006254144
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	107.0
1	555.5
2	1004.0
3	32463.5
4	63923.0
5	43507.5
6	23092.0
7	21842.5
8	20593.0
9	19813.0
10	19033.0
11	17875.5
12	16718.0
13	15481.5
14	14245.0
15	13052.0
16	11859.0
17	10863.5
18	9868.0
19	8915.0
20	7962.0
21	7299.5
22	6637.0
23	6323.0
24	6009.0
25	6182.0
26	6467.0
27	6579.0
28	7129.5
29	7680.0
30	8358.5
31	9037.0
32	9927.0
33	10817.0
34	11792.0
35	12767.0
36	13710.0
37	14653.0
38	15401.5
39	16150.0
40	16590.5
41	17031.0
42	18344.5
43	19658.0
44	20808.5
45	21959.0
46	39677.0
47	57395.0
48	46758.0
49	36121.0
50	32580.5
51	29040.0
52	24662.0
53	20284.0
54	17827.5
55	15371.0
56	14020.0
57	12669.0
58	11624.0
59	10579.0
60	9416.5
61	8254.0
62	7195.0
63	6136.0
64	5364.0
65	4592.0
66	3861.0
67	3130.0
68	2560.5
69	1991.0
70	1708.5
71	1426.0
72	1200.5
73	975.0
74	829.5
75	550.0
76	416.0
77	315.0
78	214.0
79	172.0
80	130.0
81	90.5
82	51.0
83	37.0
84	23.0
85	14.5
86	6.0
87	5.5
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	553233.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.705777784987205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.23860950738279	38.75551052720553
2	7.0083789968608095	6.686803421144266
3	3.27937185276291	4.693349546467475
4	2.212472083739948	4.221908063295421
5	1.5814767041518518	3.7722788110201098
6	1.1837710211087806	3.388363036879373
7	0.8593494951625152	2.869715523910472
8	0.6464971705607445	2.4673320285915077
9	0.45588140232513413	1.957335918806808
>10	1.4288144743230342	9.995584186279217
>50	0.05691912103448491	1.9534158373196893
>100	0.04115098613338023	3.863899457043122
>500	0.003845886554521517	1.2061384155081425
>1k	0.0019229432772607585	1.8493877743112377
>5k	0.001153765966356455	4.662732231780336
>10k+	3.8458865545215175E-4	7.656245220437297
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41730	7.542933989837916	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG	9879	1.7856852356963522	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC	8822	1.594626495527201	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC	6713	1.213412793524609	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGATCAGT	2471	0.44664725350801565	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT	2460	0.444658941169453	No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	2101	0.3797676566654556	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTC	1586	0.2866784880872978	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTA	1462	0.2642647853616831	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCT	920	0.16629521377068973	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGTGATCAGTCGTATGCCG	784	0.1417124430393704	No Hit
GAATCTTTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC	774	0.13990488636794984	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	717	0.12960181334085277	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCC	620	0.11206851362807353	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGT	604	0.10917642295380064	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	603	0.1089956672866586	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11315304763092585	0.0
2	0.0	0.0	0.0	0.6704227694298786	0.0
3	0.0	0.0	0.0	0.825872643172045	0.0
4	0.0	0.0	0.0	1.5461839767331305	0.0
5	0.0	0.0	0.0	3.1411719835946155	0.0
6	0.0	0.0	0.0	3.4283927386833395	0.0
7	0.0	0.0	0.0	3.7758051309303675	0.0
8	0.0	0.0	0.0	4.299092787306614	0.0
9	0.0	0.0	0.0	4.425983265640336	0.0
10	0.0	0.0	0.0	6.740740339061481	0.0
11	0.0	0.0	0.0	7.245048650387811	0.0
12	0.0	0.0	0.0	9.53992260042333	0.0
13	0.0	0.0	0.0	9.76875927502517	0.0
14	0.0	0.0	0.0	9.909748695395972	0.0
15	0.0	0.0	0.0	10.36362617558967	0.0
16	0.0	0.0	0.0	10.634036653634183	0.0
17	0.0	0.0	0.0	10.882214184620222	0.0
18	0.0	0.0	0.0	11.129668692937695	0.0
19	1.8075566714205408E-4	0.0	0.0	11.881973779582925	0.0
20	1.8075566714205408E-4	0.0	0.0	12.151661234958869	0.0
21	3.6151133428410817E-4	0.0	0.0	12.43345932003333	0.0
22	3.6151133428410817E-4	0.0	0.0	12.86727292117426	0.0
23	3.6151133428410817E-4	0.0	0.0	13.160458613278673	0.0
24	3.6151133428410817E-4	0.0	0.0	13.393994935226207	0.0
25	3.6151133428410817E-4	0.0	0.0	13.590114834075335	0.0
26	3.6151133428410817E-4	0.0	0.0	13.782077352580197	0.0
27	3.6151133428410817E-4	0.0	0.0	13.974762893753626	0.0
28	3.6151133428410817E-4	0.0	0.0	14.15190344755284	0.0
29	3.6151133428410817E-4	0.0	0.0	14.348746369070536	0.0
30	3.6151133428410817E-4	0.0	0.0	14.564930146972433	0.0
31	3.6151133428410817E-4	0.0	0.0	14.77063009618009	0.0
32	3.6151133428410817E-4	0.0	0.0	14.95716994467069	0.0
33	3.6151133428410817E-4	0.0	0.0	15.150397752845546	0.0
34	3.6151133428410817E-4	0.0	0.0	15.36730455341601	0.0
35	5.422670014261622E-4	0.0	0.0	15.599937097027835	0.0
36	5.422670014261622E-4	0.0	0.0	15.813771051256884	0.0
37	5.422670014261622E-4	0.0	0.0	16.025797448814515	0.0
38	5.422670014261622E-4	0.0	0.0	16.23908913604214	0.0
39	5.422670014261622E-4	0.0	0.0	16.459972561289728	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	45	3.8380676E-10	45.0	26
ATTCGGC	35	1.2103919E-7	45.0	28
TTATACG	35	1.2103919E-7	45.0	26
CTACGGG	45	3.8380676E-10	45.0	3
TATACGG	30	2.1634532E-6	44.999996	27
CGTTTTT	20115	0.0	44.574947	1
ACCGGTG	135	0.0	43.333332	17
CGACCAC	865	0.0	42.658962	12
TGATACC	1430	0.0	42.482513	4
GCGACCA	870	0.0	42.413795	11
GATACCT	1450	0.0	42.051723	5
GAATGAT	4285	0.0	41.324387	1
GCGAGAC	60	3.6379788E-12	41.249996	21
ATGATAC	4075	0.0	41.190186	3
CCACCGG	175	0.0	41.14286	15
CACCGGT	170	0.0	41.02941	16
TACGGCT	1115	0.0	40.964123	7
CGACCAA	55	6.002665E-11	40.909092	29
ACCACCG	490	0.0	40.867348	14
AATGATA	4090	0.0	40.81907	2
>>END_MODULE