##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933382.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498026
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.96706798440242	31.0	28.0	31.0	25.0	34.0
2	27.603221518555255	31.0	25.0	33.0	16.0	34.0
3	27.49708047371021	31.0	25.0	33.0	16.0	34.0
4	32.74290900475076	35.0	32.0	37.0	28.0	37.0
5	34.12285703959231	35.0	33.0	37.0	30.0	37.0
6	33.05455136880404	35.0	32.0	37.0	28.0	37.0
7	33.78975595651632	35.0	33.0	37.0	30.0	37.0
8	33.155937641809864	35.0	32.0	37.0	28.0	37.0
9	34.78307357447201	37.0	34.0	39.0	28.0	39.0
10	34.80236975579588	37.0	33.0	39.0	28.0	39.0
11	35.22411279732383	37.0	34.0	39.0	30.0	39.0
12	35.35885074273231	37.0	34.0	39.0	30.0	39.0
13	35.33695228763157	37.0	34.0	39.0	30.0	39.0
14	36.172794191467915	38.0	34.0	40.0	30.0	41.0
15	36.25447868183589	38.0	34.0	40.0	28.0	41.0
16	36.40557119507817	38.0	34.0	40.0	31.0	41.0
17	35.48811909418384	37.0	33.0	40.0	27.0	41.0
18	35.33173970836864	37.0	33.0	39.0	27.0	40.0
19	34.68277158220655	37.0	33.0	37.0	27.0	40.0
20	33.26585760582781	35.0	32.0	37.0	25.0	39.0
21	34.04426274933437	35.0	33.0	37.0	27.0	39.0
22	34.56706878757334	35.0	33.0	38.0	29.0	40.0
23	34.69103018717898	35.0	33.0	39.0	29.0	40.0
24	34.30611454020473	35.0	33.0	38.0	27.0	40.0
25	33.30506037837382	35.0	32.0	38.0	24.0	40.0
26	33.27229301281459	35.0	32.0	38.0	24.0	40.0
27	34.04981667623779	35.0	33.0	38.0	25.0	40.0
28	33.56834382140691	35.0	33.0	38.0	24.0	40.0
29	33.437625746446976	35.0	33.0	38.0	23.0	40.0
30	32.82703111885725	35.0	32.0	38.0	21.0	40.0
31	32.8475943023055	35.0	32.0	38.0	21.0	40.0
32	32.0759679213535	35.0	31.0	38.0	15.0	40.0
33	31.026615076321317	35.0	27.0	39.0	10.0	40.0
34	29.954074686863738	35.0	23.0	39.0	8.0	40.0
35	29.64297245525334	35.0	21.0	39.0	7.0	40.0
36	29.107032564564904	35.0	18.0	39.0	7.0	40.0
37	28.891559878399924	35.0	18.0	39.0	7.0	40.0
38	28.653799199238595	35.0	18.0	39.0	7.0	40.0
39	28.27035335504572	35.0	17.0	38.0	7.0	40.0
40	27.983968708461003	34.0	16.0	38.0	7.0	40.0
41	27.299972290603304	33.0	15.0	38.0	7.0	40.0
42	27.756512712187718	34.0	15.0	38.0	7.0	40.0
43	27.67829591226161	34.0	15.0	38.0	7.0	40.0
44	27.885273058033114	35.0	15.0	39.0	7.0	40.0
45	27.914928136282043	35.0	15.0	39.0	7.0	40.0
46	27.64931750551176	34.0	15.0	38.0	7.0	40.0
47	27.227379695036003	33.0	13.0	38.0	7.0	40.0
48	27.225452084830913	33.0	13.0	38.0	7.0	40.0
49	27.435774437479168	34.0	12.0	38.0	7.0	40.0
50	27.340845658660392	34.0	12.0	38.0	7.0	40.0
51	26.129308509997472	31.0	10.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	16.0
10	15.0
11	28.0
12	46.0
13	56.0
14	72.0
15	154.0
16	272.0
17	630.0
18	1307.0
19	2421.0
20	3973.0
21	5851.0
22	8269.0
23	12077.0
24	17121.0
25	24924.0
26	30815.0
27	30009.0
28	25730.0
29	22364.0
30	22503.0
31	25136.0
32	28667.0
33	34115.0
34	39090.0
35	44691.0
36	46298.0
37	41082.0
38	26721.0
39	3567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.383494034448	2.020778031669029	23.72346022095232	38.87226771293065
2	40.48322778328842	26.466489701340894	22.960046262644923	10.090236252725761
3	8.583286816350952	26.751816170240104	54.283912888082156	10.38098412532679
4	8.16744507314879	2.081618228767173	78.03588567665142	11.715051021432615
5	26.2116837273556	2.5733596237947416	56.74663571781393	14.46832093103573
6	9.953094818342818	21.668547425234827	60.26111086569777	8.11724689072458
7	39.20658760787589	0.9563757715460638	57.183962283093656	2.6530743374843886
8	36.211362458988084	17.451699308871422	41.81689309393485	4.520045138205636
9	34.33154092356624	2.014754249778124	39.76619694554099	23.88750788111464
10	26.835948323983082	11.295394216366214	47.53285973021489	14.335797729435813
11	23.133731973832692	12.975627778469399	50.6736596081329	13.216980639565001
12	15.283338620875215	11.3789239919201	53.413476404846335	19.924260982358348
13	15.07310863288262	13.361350612217032	62.8605735443531	8.70496721054724
14	13.991438198005726	22.3255412368029	48.43482067201311	15.248199893178269
15	8.651957929907274	13.94083843012212	64.19524281864803	13.211960821322583
16	9.298510519531108	16.902731985880255	48.50288940738034	25.2958680872083
17	10.452867922558259	17.999261082754717	57.02674157574103	14.521129418946
18	10.489612992092782	16.524840068590795	54.07830113287259	18.90724580644384
19	10.722733351270817	19.937513302518344	49.52030616875424	19.8194471774566
20	13.444679595041222	17.468967483625352	58.99009288671676	10.096260034616666
21	13.587242433125981	25.498668744202106	50.13131844522174	10.782770377450174
22	10.599245822507259	13.614349451635055	55.08246557408649	20.70393915177119
23	12.294137253878311	23.82325420761165	52.085433290631414	11.797175247878625
24	15.103227542337146	16.380670888668465	48.27840313557927	20.237698433415122
25	9.431636099320116	29.98136643468413	46.52849449627128	14.058502969724474
26	11.348604289735876	15.472485372249642	54.3124254556991	18.866484882315383
27	17.30251031070667	19.27610205089694	46.30139791898415	17.11998971941224
28	9.135266030287575	20.201756534799387	55.14270339299555	15.520274041917489
29	17.12741905041102	18.173549172131576	47.326444804086535	17.37258697337087
30	16.75695646412035	23.62627653977905	48.74343909755716	10.873327898543451
31	15.57248015163867	21.06355893065824	43.50696549979318	19.85699541790991
32	19.915225309521993	22.34501813158349	41.44201306759085	16.297743491303667
33	18.36731415628903	18.79721139056997	39.31260616915583	23.522868283985172
34	18.458273262841697	29.798444257930306	37.81609795472525	13.927184524502737
35	24.01681839903941	20.42925469754591	36.17622373129114	19.377703172123546
36	15.594567351905322	34.93271435627859	33.7361904800151	15.73652781180099
37	19.588133952845833	21.33442832301928	39.35637898422974	19.721058739905146
38	22.738973467248698	26.163091886768964	32.886234855208365	18.211699790773974
39	18.305269202812706	27.53912446338143	34.60502062141334	19.550585712392525
40	26.436571584616065	20.76136587246449	36.95730744981186	15.844755093107587
41	16.339106793621216	35.357190186857714	32.385658580074136	15.918044439446938
42	20.579849244818543	21.424785051382862	41.04745535373655	16.947910350062045
43	19.647970186295495	27.054210021163556	32.55372209483039	20.74409769771056
44	21.32117600285929	22.178761751394504	35.53870681450366	20.961355431242545
45	16.082292892338952	21.81974435069655	36.29930967459531	25.798653082369192
46	23.647962154586306	26.015308437712086	31.61581925441644	18.72091015328517
47	15.656009927192557	20.436684028544693	45.82310963684627	18.08419640741648
48	19.352202495452044	25.784396798560717	31.776855023633306	23.086545682353936
49	16.7661929296864	19.794950464433583	41.48458112628658	21.954275479593434
50	18.802231208812394	20.857947175448672	36.839642910209506	23.500178705529432
51	17.824972993377855	20.85112022263898	33.445643400143766	27.878263383839396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16.0
1	598.0
2	1180.0
3	15670.0
4	30160.0
5	23441.0
6	16722.0
7	16350.0
8	15978.0
9	15896.0
10	15814.0
11	15333.0
12	14852.0
13	14263.0
14	13674.0
15	13018.0
16	12362.0
17	11794.5
18	11227.0
19	10425.0
20	9623.0
21	9072.5
22	8522.0
23	7987.5
24	7453.0
25	7080.0
26	6451.0
27	6195.0
28	6237.5
29	6280.0
30	6241.5
31	6203.0
32	6573.0
33	6943.0
34	7722.5
35	8502.0
36	9067.5
37	9633.0
38	10350.5
39	11068.0
40	11921.0
41	12774.0
42	13532.5
43	14291.0
44	17759.0
45	21227.0
46	65441.0
47	109655.0
48	72292.5
49	34930.0
50	30161.0
51	25392.0
52	20472.5
53	15553.0
54	13716.0
55	11879.0
56	10514.0
57	9149.0
58	8087.0
59	7025.0
60	6187.0
61	5349.0
62	4597.5
63	3846.0
64	3309.0
65	2772.0
66	2291.5
67	1811.0
68	1508.5
69	1206.0
70	987.5
71	769.0
72	653.5
73	538.0
74	428.0
75	265.0
76	212.0
77	160.5
78	109.0
79	83.0
80	57.0
81	40.5
82	24.0
83	19.5
84	15.0
85	12.0
86	9.0
87	5.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	498026.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.657494947210864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.86844252523484	42.268008032436924
2	5.892112017056745	5.733908213966781
3	2.288442245526637	3.3404960099608867
4	1.21555262014315	2.36582981890737
5	0.8153457414709763	1.9836340647926962
6	0.5585045475177939	1.630525931930481
7	0.41598341742140027	1.4168497721913706
8	0.34791557261295947	1.3542960173176841
9	0.24655115451749351	1.0796905399647554
>10	1.2446990531846338	9.760020666437374
>50	0.06136593129854838	2.033873590696089
>100	0.0375709783456959	3.8022650298575518
>500	0.0016698212598087066	0.6174948282903444
>1k	0.004174553149521767	6.291541056403002
>5k	0.0	0.0
>10k+	0.0016698212598087066	16.321566426846726
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCG	22216	4.460811282945067	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGC	21080	4.232710742009453	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTC	19895	3.9947713573186947	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17162	3.4460048270572217	No Hit
GAACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCT	4848	0.9734431535702956	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGACGAGAT	4834	0.9706320553545398	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTC	4222	0.8477469047800718	No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	3984	0.799958235112223	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGACGAGATCGTA	3894	0.7818868894395071	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCT	3528	0.7083967503704626	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGACGAGATCGT	1543	0.30982318192222896	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCT	1456	0.2923542144386036	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTT	1409	0.282916956142852	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCTGTCTC	1256	0.25219566849923497	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTAT	986	0.19798163148108733	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATG	948	0.19035150775260729	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCAGACGAGAT	558	0.11204234317083847	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGACGAGA	548	0.11003441587387004	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0079272969684314E-4	0.0	0.0	0.11384947773811006	0.0
2	2.0079272969684314E-4	0.0	0.0	1.1760430178344103	0.0
3	2.0079272969684314E-4	0.0	0.0	1.3463152526173332	0.0
4	2.0079272969684314E-4	0.0	0.0	2.645845799215302	0.0
5	2.0079272969684314E-4	0.0	0.0	7.733933569733307	0.0
6	2.0079272969684314E-4	0.0	0.0	8.224671001112391	0.0
7	2.0079272969684314E-4	0.0	0.0	9.227630685948123	0.0
8	2.0079272969684314E-4	0.0	0.0	10.424556147671005	0.0
9	2.0079272969684314E-4	0.0	0.0	10.612096557207856	0.0
10	2.0079272969684314E-4	0.0	0.0	16.313806909679414	0.0
11	2.0079272969684314E-4	0.0	0.0	16.66258388116283	0.0
12	2.0079272969684314E-4	0.0	0.0	21.826370510776545	0.0
13	2.0079272969684314E-4	0.0	0.0	22.229160726548415	0.0
14	2.0079272969684314E-4	0.0	0.0	22.52392445374338	0.0
15	2.0079272969684314E-4	0.0	0.0	23.569251404545145	0.0
16	2.0079272969684314E-4	0.0	0.0	24.07203639970604	0.0
17	2.0079272969684314E-4	0.0	0.0	24.32965347190709	0.0
18	2.0079272969684314E-4	0.0	0.0	24.554340536437856	0.0
19	2.0079272969684314E-4	0.0	0.0	25.909289876432155	0.0
20	2.0079272969684314E-4	0.0	0.0	26.191604454385917	0.0
21	2.0079272969684314E-4	0.0	0.0	26.38797974402943	0.0
22	2.0079272969684314E-4	0.0	0.0	26.77651367599282	0.0
23	2.0079272969684314E-4	0.0	0.0	27.01184275519752	0.0
24	2.0079272969684314E-4	0.0	0.0	27.200587921112554	0.0
25	2.0079272969684314E-4	0.0	0.0	27.349375333817914	0.0
26	2.0079272969684314E-4	0.0	0.0	27.532899888760827	0.0
27	2.0079272969684314E-4	0.0	0.0	27.771642444370375	0.0
28	2.0079272969684314E-4	0.0	0.0	27.93026870083088	0.0
29	2.0079272969684314E-4	0.0	0.0	28.11921465947561	0.0
30	2.0079272969684314E-4	0.0	0.0	28.290691650636713	0.0
31	2.0079272969684314E-4	0.0	0.0	28.48626376936144	0.0
32	2.0079272969684314E-4	0.0	0.0	28.673603386168594	0.0
33	2.0079272969684314E-4	0.0	0.0	28.8639548939212	0.0
34	2.0079272969684314E-4	0.0	0.0	29.10892202415135	0.0
35	2.0079272969684314E-4	0.0	0.0	29.310317132037284	0.0
36	2.0079272969684314E-4	0.0	0.0	29.50709400714019	0.0
37	2.0079272969684314E-4	0.0	0.0	29.729973937103686	0.0
38	2.0079272969684314E-4	0.0	0.0	29.96711015087566	0.0
39	2.0079272969684314E-4	0.0	0.0	30.2124788665652	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTAG	20	7.02943E-4	45.000004	1
ATGGCGT	20	7.02943E-4	45.000004	38
TAGCGGG	20	7.02943E-4	45.000004	3
TTCCGTC	20	7.02943E-4	45.000004	42
TATTCTA	20	7.02943E-4	45.000004	19
CGGGCTA	20	7.02943E-4	45.000004	6
TCATGTA	20	7.02943E-4	45.000004	24
AACGCAG	20	7.02943E-4	45.000004	37
ACTACCG	40	6.8012014E-9	45.000004	14
CGAATGG	20	7.02943E-4	45.000004	29
CGGCTAT	20	7.02943E-4	45.000004	31
CGATGCG	30	2.1630149E-6	45.000004	10
CGATCAC	20	7.02943E-4	45.000004	34
TATCGGG	20	7.02943E-4	45.000004	3
TAATGCG	20	7.02943E-4	45.000004	24
CTTACGG	20	7.02943E-4	45.000004	2
GCATACT	20	7.02943E-4	45.000004	18
CACATCC	25	3.8876104E-5	45.0	45
GGCAACG	25	3.8876104E-5	45.0	8
CGCGCAA	25	3.8876104E-5	45.0	16
>>END_MODULE