SRR2933381.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933381.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	294654
Sequences flagged as poor quality	0
Sequence length	51
%GC	34

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16842	5.715856563969944	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCG	9255	3.140972123236067	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGC	7348	2.493772356730267	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTC	4762	1.616132820189103	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGACGAGAT	2066	0.7011613621400015	No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	1724	0.5850930243607757	Illumina Single End Adapter 2 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCT	1564	0.5307920476219566	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTC	1234	0.41879628309814226	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGACGAGATCGTA	1221	0.41438432873811315	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGACGAGATCGTATGCCG	803	0.2725230270079483	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCT	732	0.24842696858009733	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCT	727	0.24673006305700923	No Hit
GAATCTTTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTC	485	0.16459983573954537	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGACGAGATCGT	467	0.1584909758564282	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCAGACGAGATCGTATGC	446	0.1513639726594582	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTT	407	0.13812810957937108	No Hit
GAATGACTCTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCT	403	0.13677058516090057	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	386	0.13100110638240106	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCC	369	0.12523162760390152	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	365	0.12387410318543104	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCTGTCTC	364	0.12353472208081342	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT	356	0.12081967324387248	No Hit
GAATCTGTCTCTTATCCACATCTGACGCAGACGAGATCGTATGCCGTCTTC	355	0.12048029213925485	No Hit
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT	327	0.11097762120996152	Illumina Single End Adapter 2 (95% over 23bp)
GAATCTGTCTCTTATCCCCATCTGACGCAGACGAGATCGTATGCCGTCTTC	298	0.10113556917605054	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	20	7.02474E-4	45.0	6
GGGCTTA	25	3.883723E-5	45.0	7
CTCGTCC	20	7.02474E-4	45.0	37
CGTGCGG	35	1.2078272E-7	45.0	2
CGTCTCG	20	7.02474E-4	45.0	42
CGATTCG	20	7.02474E-4	45.0	10
CCTCGTC	20	7.02474E-4	45.0	36
TACGGAT	25	3.883723E-5	45.0	29
TGTTGCG	25	3.883723E-5	45.0	1
ACGGATC	25	3.883723E-5	45.0	30
TCGTCCC	20	7.02474E-4	45.0	38
TAGGGTA	20	7.02474E-4	45.0	5
GAACGTT	20	7.02474E-4	45.0	13
GATGCAT	20	7.02474E-4	45.0	20
CCGGTGA	35	1.2078272E-7	45.0	18
TGGGCGC	20	7.02474E-4	45.0	6
CACCGGT	165	0.0	45.0	16
CCATTCT	20	7.02474E-4	45.0	44
GCGACCT	120	0.0	44.999996	11
GATCCGT	60	0.0	44.999996	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content