##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933381.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	294654
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.902492414832313	31.0	25.0	31.0	16.0	33.0
2	27.678768996857332	31.0	26.0	33.0	16.0	34.0
3	27.777983668981246	31.0	25.0	33.0	16.0	34.0
4	31.548188723044657	35.0	28.0	37.0	19.0	37.0
5	33.60021245257149	35.0	33.0	37.0	28.0	37.0
6	31.801557080507987	35.0	32.0	37.0	17.0	37.0
7	32.54387518920497	35.0	32.0	35.0	27.0	37.0
8	32.36558811351619	35.0	32.0	37.0	21.0	37.0
9	33.20864471549682	35.0	32.0	38.0	26.0	39.0
10	32.252017620666955	35.0	28.0	39.0	17.0	39.0
11	32.971851731183015	37.0	31.0	39.0	17.0	39.0
12	33.7406585350954	37.0	31.0	39.0	25.0	39.0
13	33.88800423547619	35.0	32.0	39.0	27.0	39.0
14	34.011881732472666	37.0	32.0	40.0	23.0	41.0
15	34.37081118871626	37.0	32.0	40.0	25.0	41.0
16	34.582917591480175	37.0	32.0	40.0	25.0	41.0
17	32.56946791830418	35.0	27.0	40.0	18.0	41.0
18	33.152952276229065	36.0	30.0	39.0	23.0	39.0
19	32.89281665953966	36.0	31.0	37.0	23.0	39.0
20	31.926602048504346	34.0	30.0	35.0	23.0	39.0
21	32.47498421877864	35.0	31.0	36.0	23.0	39.0
22	32.67734020240689	34.0	30.0	37.0	25.0	39.0
23	33.434937248433755	35.0	32.0	38.0	26.0	39.0
24	31.980655277036796	34.0	30.0	37.0	21.0	39.0
25	31.152823311409314	34.0	27.0	36.0	18.0	39.0
26	30.24640086338553	33.0	25.0	36.0	18.0	39.0
27	32.10526583721925	34.0	30.0	37.0	22.0	39.0
28	32.00297297847645	35.0	30.0	37.0	21.0	39.0
29	32.01788877802439	35.0	30.0	37.0	21.0	39.0
30	31.092895395955935	34.0	27.0	37.0	18.0	39.0
31	31.47179403639523	34.0	30.0	37.0	19.0	39.0
32	31.155416183048594	35.0	30.0	37.0	16.0	39.0
33	30.556615555872312	35.0	27.0	37.0	12.0	40.0
34	29.034128163880347	34.0	22.0	37.0	8.0	39.0
35	28.50467667162163	34.0	21.0	37.0	7.0	39.0
36	27.81203377520753	33.0	18.0	37.0	7.0	40.0
37	27.75546233887882	34.0	16.0	38.0	7.0	40.0
38	27.6344627936495	33.0	17.0	38.0	7.0	40.0
39	27.098060776368214	33.0	15.0	37.0	7.0	39.0
40	26.960838814338175	33.0	15.0	37.0	7.0	39.0
41	26.117293503566895	31.0	15.0	37.0	7.0	39.0
42	26.90261798584102	33.0	15.0	37.0	7.0	39.0
43	26.809128672952006	33.0	15.0	37.0	7.0	40.0
44	26.89000658399343	33.0	15.0	37.0	7.0	40.0
45	27.112786522497572	33.0	15.0	37.0	7.0	40.0
46	26.84418334724796	33.0	14.0	37.0	7.0	40.0
47	26.6062839805331	33.0	12.0	37.0	7.0	40.0
48	26.484480102085836	33.0	12.0	37.0	7.0	39.0
49	26.42589952961779	33.0	11.0	37.0	7.0	39.0
50	26.52724890888975	33.0	11.0	37.0	7.0	40.0
51	24.779863840300827	29.0	10.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	11.0
11	16.0
12	29.0
13	41.0
14	96.0
15	211.0
16	421.0
17	791.0
18	1370.0
19	2196.0
20	3352.0
21	4754.0
22	6477.0
23	8965.0
24	11894.0
25	16266.0
26	19753.0
27	19761.0
28	17868.0
29	17506.0
30	18513.0
31	20783.0
32	22909.0
33	24813.0
34	25553.0
35	24527.0
36	16290.0
37	7842.0
38	1613.0
39	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.12926347512676	2.1778085483312632	28.115688230942055	41.577239745599925
2	44.77692479993484	18.643222219959682	25.71694258350472	10.862910396600759
3	9.566474576961454	18.695147528966178	60.40746095420392	11.330916939868455
4	8.943031487778887	2.224303759663877	75.9148017674968	12.917862985060443
5	21.85919756731624	2.859964568612678	61.78161504680065	13.499222817270425
6	10.741072580043033	15.957020776911223	63.655338125394536	9.646568517651211
7	41.0990517691937	0.9906534443788308	55.20983933698508	2.700455449442397
8	40.95481479973121	12.913790411805032	41.74726967901335	4.384125109450406
9	37.9903208508963	2.049183109681185	41.20595681714825	18.75453922227426
10	25.13761903792244	12.523502141494768	47.90330353567235	14.435575284910437
11	21.19944069993959	14.220068283478248	52.69129215961772	11.88919885696444
12	15.709272570540362	12.470558689174421	54.26398419841577	17.556184541869445
13	14.946343847359955	14.219728902373632	61.74054993314192	9.093377317124492
14	13.453745749251663	19.063376027476295	51.72677106029445	15.756107162977596
15	9.124600378749314	14.442023525898174	62.67384797084038	13.75952812451214
16	9.989682814419625	14.361590204103797	51.8027924277288	23.845934553747785
17	11.02818899454954	16.455232238489888	57.884841203581146	14.63173756337942
18	11.048551860826597	14.441005382584319	56.3372633665248	18.173179390064277
19	11.884108140395174	18.84752964493949	51.250279989411304	18.018082225254027
20	13.79075118613696	16.15997067747256	59.19756731624211	10.851710820148378
21	14.522796228797166	22.508773001554367	51.18851262837091	11.779918141277566
22	11.397096255268892	14.190881508481134	55.75624291541944	18.655779320830533
23	12.347702729302844	21.974247761781616	52.403836364006594	13.274213144908945
24	13.320029594032324	15.57284136648408	51.709802005063565	19.39732703442003
25	10.14851317138067	24.427633767062385	49.71050791776117	15.713345143795774
26	11.186001208196734	16.345951522803016	56.11055678864023	16.357490480360013
27	14.231607241035249	18.38834701039185	50.418117520888906	16.961928227683995
28	9.64962294759277	19.22695771990199	56.59824743597576	14.525171896529491
29	16.05068996178569	16.555349664352086	50.776503967365116	16.617456406497112
30	14.725746129358502	22.114412157988692	51.18240376848778	11.97743794416502
31	14.604926456114628	19.43058638267256	47.234383378471016	18.730103782741793
32	18.080188967399053	21.45567343392589	45.55410752950919	14.91003006916587
33	15.848758204538205	18.359499616499352	44.069993959016344	21.721748219946107
34	16.819388163744595	26.9916580124485	41.34238802120453	14.846565802602374
35	20.277681619798134	20.93811724938402	39.748993735024804	19.035207395793034
36	15.694339801937188	30.124824370278358	37.38384681694462	16.796989010839834
37	17.920001086019536	21.669144148730375	40.82245616893034	19.58839859631975
38	21.415287082476393	24.44256653566556	36.949439003034065	17.192707378823975
39	16.710786210266956	26.38314769186911	37.86984055875705	19.036225539106884
40	24.470395786244207	20.41682787269136	38.74340752204281	16.36936881902163
41	16.286559829494934	30.765915276901048	36.45258506587387	16.49493982773015
42	19.143809349270672	21.177720309244062	42.08291759148018	17.59555275000509
43	17.945454668865857	25.14304913559633	36.56661711702539	20.344879078512424
44	20.546132073550673	21.711906167912197	38.10910423751247	19.63285752102466
45	16.15997067747256	22.090655480665458	38.54724524357382	23.20212859828816
46	20.393749957577363	25.348374703889988	34.65047140035431	19.60740393817834
47	15.981116835339076	20.54545331134144	44.80203900167654	18.671390851642943
48	18.59774515194092	24.55931363565402	34.82661019365086	22.0163310187542
49	16.976521615182552	19.634893807652364	40.59642835325501	22.79215622391008
50	17.811059751437277	20.714804482545627	37.849477692479994	23.6246580735371
51	17.793072552892543	20.44567526658386	36.205515621712244	25.555736558811354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32.0
1	174.5
2	317.0
3	12242.0
4	24167.0
5	17584.5
6	11002.0
7	10771.5
8	10541.0
9	10493.0
10	10445.0
11	9826.0
12	9207.0
13	8732.0
14	8257.0
15	7663.0
16	7069.0
17	6602.0
18	6135.0
19	5724.5
20	5314.0
21	4837.0
22	4360.0
23	4168.0
24	3976.0
25	3724.0
26	3370.0
27	3268.0
28	3289.0
29	3310.0
30	3243.5
31	3177.0
32	3538.0
33	3899.0
34	4439.0
35	4979.0
36	5263.5
37	5548.0
38	5967.0
39	6386.0
40	7236.0
41	8086.0
42	8904.5
43	9723.0
44	11515.0
45	13307.0
46	28091.0
47	42875.0
48	33816.5
49	24758.0
50	21854.0
51	18950.0
52	15417.5
53	11885.0
54	10037.5
55	8190.0
56	7099.5
57	6009.0
58	5182.5
59	4356.0
60	3893.0
61	3430.0
62	2992.0
63	2554.0
64	2204.5
65	1855.0
66	1571.0
67	1287.0
68	1107.5
69	928.0
70	750.5
71	573.0
72	511.5
73	450.0
74	351.5
75	202.0
76	151.0
77	110.0
78	69.0
79	63.5
80	58.0
81	42.0
82	26.0
83	19.5
84	13.0
85	8.0
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	294654.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.12877104065209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.77347535792418	46.10168104692821
2	6.188285298342063	6.575519854996976
3	2.2677272200341188	3.6144468076754146
4	1.2100707950587963	2.571582968546345
5	0.7566801151962955	2.0100742295639114
6	0.5483731324726644	1.7480634359991216
7	0.40989843245905594	1.5244179977627563
8	0.3360916663804865	1.4284909753440076
9	0.2670946004198207	1.27713670847092
>10	1.1292508598388697	9.432166652512592
>50	0.061724078034499134	2.265150186919074
>100	0.04353171819275203	4.786087677786187
>500	0.0019491814116157622	0.7808244015255937
>1k	0.0038983628232315245	4.339409173995685
>5k	0.0012994542744105082	5.731223491834409
>10k+	6.497271372052541E-4	5.813724390138837
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16842	5.715856563969944	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCG	9255	3.140972123236067	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGC	7348	2.493772356730267	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTC	4762	1.616132820189103	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGACGAGAT	2066	0.7011613621400015	No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	1724	0.5850930243607757	Illumina Single End Adapter 2 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCT	1564	0.5307920476219566	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTC	1234	0.41879628309814226	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGACGAGATCGTA	1221	0.41438432873811315	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGACGAGATCGTATGCCG	803	0.2725230270079483	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCT	732	0.24842696858009733	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCT	727	0.24673006305700923	No Hit
GAATCTTTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTC	485	0.16459983573954537	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGACGAGATCGT	467	0.1584909758564282	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCAGACGAGATCGTATGC	446	0.1513639726594582	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTT	407	0.13812810957937108	No Hit
GAATGACTCTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCT	403	0.13677058516090057	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	386	0.13100110638240106	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCC	369	0.12523162760390152	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	365	0.12387410318543104	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCTGTCTC	364	0.12353472208081342	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT	356	0.12081967324387248	No Hit
GAATCTGTCTCTTATCCACATCTGACGCAGACGAGATCGTATGCCGTCTTC	355	0.12048029213925485	No Hit
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT	327	0.11097762120996152	Illumina Single End Adapter 2 (95% over 23bp)
GAATCTGTCTCTTATCCCCATCTGACGCAGACGAGATCGTATGCCGTCTTC	298	0.10113556917605054	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16222416800722203	0.0
2	0.0	0.0	0.0	1.0968797301241455	0.0
3	0.0	0.0	0.0	1.3466642231227135	0.0
4	0.0	0.0	0.0	2.338335810815397	0.0
5	0.0	0.0	0.0	4.744547842554318	0.0
6	0.0	0.0	0.0	5.218663245705132	0.0
7	0.0	0.0	0.0	5.801041221228967	0.0
8	0.0	0.0	0.0	6.648475839459162	0.0
9	0.0	0.0	0.0	6.88977580484229	0.0
10	0.0	0.0	0.0	11.49857120554956	0.0
11	0.0	0.0	0.0	12.271681361868497	0.0
12	0.0	0.0	0.0	15.77103993158077	0.0
13	0.0	0.0	0.0	16.153183055380207	0.0
14	3.393811046176193E-4	0.0	0.0	16.374459535590898	0.0
15	3.393811046176193E-4	0.0	0.0	17.090214285229454	0.0
16	3.393811046176193E-4	0.0	0.0	17.53276724565083	0.0
17	3.393811046176193E-4	0.0	0.0	17.975659587176825	0.0
18	3.393811046176193E-4	0.0	0.0	18.381898769404113	0.0
19	3.393811046176193E-4	0.0	0.0	19.591113645156693	0.0
20	3.393811046176193E-4	0.0	0.0	20.000067876220925	0.0
21	3.393811046176193E-4	0.0	0.0	20.45246288867621	0.0
22	3.393811046176193E-4	0.0	0.0	21.055882492686337	0.0
23	3.393811046176193E-4	0.0	0.0	21.532373563569475	0.0
24	3.393811046176193E-4	0.0	0.0	21.899244537661122	0.0
25	3.393811046176193E-4	0.0	0.0	22.199936196352333	0.0
26	3.393811046176193E-4	0.0	0.0	22.491464565218866	0.0
27	3.393811046176193E-4	0.0	0.0	22.784350458503873	0.0
28	3.393811046176193E-4	0.0	0.0	23.064679250918026	0.0
29	3.393811046176193E-4	0.0	0.0	23.382339964840117	0.0
30	3.393811046176193E-4	0.0	0.0	23.70984273079612	0.0
31	3.393811046176193E-4	0.0	0.0	24.02478839588127	0.0
32	3.393811046176193E-4	0.0	0.0	24.33532210660639	0.0
33	3.393811046176193E-4	0.0	0.0	24.64653457954075	0.0
34	3.393811046176193E-4	0.0	0.0	24.932293469628785	0.0
35	3.393811046176193E-4	0.0	0.0	25.25640242453861	0.0
36	3.393811046176193E-4	0.0	0.0	25.57576004398379	0.0
37	3.393811046176193E-4	0.0	0.0	25.877469845988855	0.0
38	3.393811046176193E-4	0.0	0.0	26.2019181820033	0.0
39	3.393811046176193E-4	0.0	0.0	26.5409599055163	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	20	7.02474E-4	45.0	6
GGGCTTA	25	3.883723E-5	45.0	7
CTCGTCC	20	7.02474E-4	45.0	37
CGTGCGG	35	1.2078272E-7	45.0	2
CGTCTCG	20	7.02474E-4	45.0	42
CGATTCG	20	7.02474E-4	45.0	10
CCTCGTC	20	7.02474E-4	45.0	36
TACGGAT	25	3.883723E-5	45.0	29
TGTTGCG	25	3.883723E-5	45.0	1
ACGGATC	25	3.883723E-5	45.0	30
TCGTCCC	20	7.02474E-4	45.0	38
TAGGGTA	20	7.02474E-4	45.0	5
GAACGTT	20	7.02474E-4	45.0	13
GATGCAT	20	7.02474E-4	45.0	20
CCGGTGA	35	1.2078272E-7	45.0	18
TGGGCGC	20	7.02474E-4	45.0	6
CACCGGT	165	0.0	45.0	16
CCATTCT	20	7.02474E-4	45.0	44
GCGACCT	120	0.0	44.999996	11
GATCCGT	60	0.0	44.999996	5
>>END_MODULE