FastQCFastQC Report
Sat 14 Jan 2017
SRR2933380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284808
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCG120454.229164911098003No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGC104503.6691385073452993No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85513.0023735288334596No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTC76662.6916378753405805No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAACGACGT22890.8036993342883627No Hit
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC20270.7117075363051599No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCT18090.6351647425634112No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTC17790.6246313305806017No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAACGACGTCGTA17370.6098845538046684No Hit
GAACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCT15960.5603775174854638No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAACGACGTCGT6930.2433218168028988No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTAT4680.16432122693182777No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTT4380.1537878149490183No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATG4170.14641442656105166No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2970.10428077862981378No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAA207.0243434E-445.031
CTGTCGG207.0243434E-445.02
CGCGGGT207.0243434E-445.04
TTCCGCG207.0243434E-445.01
TAACGCT207.0243434E-445.042
TCGATCA253.8833925E-545.017
CCTCGAT253.8833925E-545.015
ATGTACC207.0243434E-445.020
GCCTCGA253.8833925E-545.014
GCGATGC253.8833925E-545.09
CTCGATC253.8833925E-545.016
CGTCGGG207.0243434E-445.03
GCCGTCG253.8833925E-545.040
CAAACGC302.1597334E-644.99999635
TTATACG302.1597334E-644.99999626
TTACACG1200.044.99999634
TACACGC1200.044.99999635
CGTTTTT75900.044.2588921
ACGGCTG13550.043.6715858
TGATACC13200.043.6363644
Produced by FastQC (version 0.11.5)