##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933379.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	185849
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.3411533018741	31.0	26.0	31.0	16.0	33.0
2	28.309972074103168	31.0	28.0	33.0	16.0	34.0
3	28.465576893069105	31.0	28.0	33.0	16.0	34.0
4	31.888264128405318	35.0	28.0	37.0	19.0	37.0
5	33.72463666740203	35.0	33.0	37.0	30.0	37.0
6	32.294771561859356	35.0	32.0	37.0	17.0	37.0
7	32.39658001926295	35.0	32.0	35.0	26.0	37.0
8	32.65753380432502	35.0	32.0	36.0	25.0	37.0
9	33.23185489295073	35.0	32.0	37.0	26.0	39.0
10	32.06141545017729	35.0	28.0	39.0	15.0	39.0
11	32.53640858976912	37.0	29.0	39.0	17.0	39.0
12	33.42331677867516	35.0	31.0	39.0	24.0	39.0
13	33.51578431952822	35.0	32.0	39.0	25.0	39.0
14	33.694079602257744	36.0	32.0	40.0	21.0	41.0
15	34.222971336945584	37.0	32.0	40.0	25.0	41.0
16	34.51899122405824	37.0	32.0	40.0	25.0	41.0
17	32.52142330601725	36.0	27.0	40.0	18.0	41.0
18	33.18397193420465	36.0	30.0	39.0	23.0	39.0
19	32.91256342514622	36.0	31.0	37.0	22.0	39.0
20	32.236805148265525	34.0	30.0	36.0	23.0	39.0
21	32.654574412560734	35.0	31.0	37.0	24.0	39.0
22	32.88289955824352	34.0	30.0	37.0	25.0	39.0
23	33.73701230568903	35.0	32.0	38.0	27.0	40.0
24	32.21716554837529	35.0	31.0	37.0	21.0	39.0
25	31.40742215454482	34.0	29.0	37.0	18.0	39.0
26	30.552513061679104	33.0	25.0	37.0	18.0	39.0
27	32.32656081012004	34.0	30.0	37.0	22.0	39.0
28	32.262035308234104	35.0	31.0	37.0	21.0	39.0
29	32.3436338102438	35.0	30.0	38.0	21.0	40.0
30	31.363397166516904	34.0	29.0	37.0	18.0	40.0
31	31.749576268906477	35.0	30.0	37.0	20.0	39.0
32	31.52142330601725	35.0	30.0	37.0	18.0	39.0
33	31.211149912025355	35.0	30.0	38.0	15.0	40.0
34	30.079263272872062	35.0	25.0	38.0	10.0	40.0
35	29.563188394879713	35.0	24.0	38.0	8.0	40.0
36	28.84416381040522	34.0	22.0	38.0	7.0	40.0
37	28.90036535036508	34.0	21.0	38.0	7.0	40.0
38	28.737916265355206	34.0	21.0	38.0	7.0	40.0
39	28.23839245839364	33.0	20.0	37.0	7.0	40.0
40	28.022233103218205	33.0	20.0	37.0	7.0	40.0
41	27.329353399803065	33.0	18.0	37.0	7.0	40.0
42	27.97090648860096	33.0	20.0	37.0	7.0	40.0
43	27.810324510758733	33.0	18.0	37.0	7.0	40.0
44	27.809581972461515	33.0	18.0	37.0	7.0	40.0
45	28.120355772697188	34.0	20.0	38.0	7.0	40.0
46	27.736339716651692	33.0	18.0	38.0	7.0	40.0
47	27.555967478974868	33.0	18.0	38.0	7.0	40.0
48	27.4977427911907	33.0	18.0	37.0	7.0	40.0
49	27.46014237364742	33.0	18.0	37.0	7.0	39.0
50	27.49695182648279	33.0	18.0	37.0	7.0	40.0
51	25.907231139258215	31.0	15.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	6.0
11	5.0
12	10.0
13	28.0
14	34.0
15	87.0
16	248.0
17	415.0
18	746.0
19	1185.0
20	1763.0
21	2561.0
22	3446.0
23	4786.0
24	6462.0
25	8721.0
26	11212.0
27	11817.0
28	11345.0
29	11321.0
30	11732.0
31	13095.0
32	14312.0
33	15273.0
34	16866.0
35	17516.0
36	12729.0
37	6649.0
38	1437.0
39	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.293388718798592	2.294335724163165	30.262740181545233	41.14953537549301
2	42.847688176960865	15.082136573239566	29.711755242158954	12.35842000764061
3	11.802054355955642	14.98797410801242	60.96239420174443	12.247577334287513
4	10.548348390359916	2.66345258785358	72.58042819708473	14.207770824701774
5	20.880392146312328	3.304833493857917	61.36756183783609	14.447212521993661
6	12.300308314814714	13.850491528068487	63.00706487524819	10.842135281868613
7	46.74601423736474	1.1025079500024213	49.54828920252463	2.603188610108206
8	47.28623775215363	11.177353658077257	37.770448051913114	3.7659605378560017
9	43.908226571033474	2.5843561170627765	37.81618410645201	15.691233205451738
10	26.041571383219708	16.7824416596269	43.74357677469343	13.432410182459954
11	23.34206802296488	15.429192516505335	49.80279689425286	11.425942566276923
12	18.50534573766875	13.838115889781488	51.01507137514864	16.641466997401118
13	16.34714203466255	14.757679621628311	58.0659567713574	10.829221572351749
14	14.382105903179463	19.152107355971783	49.78181211628795	16.6839746245608
15	10.613455009174116	15.128410699008334	59.48700288944251	14.771131402375046
16	11.95701887015803	14.56720240625454	50.781010390155444	22.69476833343198
17	12.528450516279346	16.023223154281162	56.11598663431066	15.33233969512884
18	12.19484635376031	14.881436004498275	54.474331311979086	18.449386329762334
19	13.495364516354675	17.736441950185366	50.783700746304795	17.984492787155162
20	15.754187539346459	16.073801849888888	56.657286291559274	11.514724319205376
21	16.252441498205535	22.224494078526117	49.26526373561332	12.257800687655033
22	12.923932870233362	15.120339630560295	53.66184375487627	18.293883744330074
23	13.620735112914248	22.105042265495108	50.1751421853225	14.099080436268153
24	14.635537452447956	15.590613885466158	50.63196465948162	19.141884002604264
25	12.233587482310908	22.64149928167491	48.60020769549473	16.524705540519456
26	11.988765072720327	17.463101765411707	53.76084886117224	16.787284300695728
27	13.37214620471458	20.192737114539224	49.70917250025558	16.72594418049061
28	10.763038811077811	20.08566093979521	54.872504022082445	14.278796227044536
29	15.569629107501251	17.34956873590926	49.82701009959698	17.253792056992502
30	12.765201857421884	23.04935727391592	51.18886838239646	12.99657248626573
31	15.289832067969158	19.869356305387704	46.23753692513815	18.603274701504986
32	17.734289665265887	22.088362057369153	45.01988173194367	15.157466545421283
33	16.485964411968858	18.50695995135836	43.84850066451797	21.158574972154813
34	16.224999865482193	25.972160194566555	41.85817518523102	15.94466475472023
35	13.790227550323111	24.703926305764355	40.91816474664916	20.587681397263367
36	16.988522940666886	27.913521191935388	38.171311118165825	16.926644749231905
37	14.711943567089412	25.2748198806558	40.338123960849934	19.67511259140485
38	20.372990976545474	25.36736813219334	37.16619406076976	17.09344683049142
39	17.1746955862017	25.798901258548607	37.8463160953247	19.180087059924993
40	19.24788403488854	21.639610651658067	38.26547358339297	20.847031730060426
41	19.91294007500713	25.54600778050998	36.85572696113512	17.68532518334777
42	15.751497183197111	21.576118246533476	41.00533228588801	21.667052284381406
43	20.40688946402725	22.20350930056121	36.65933096223278	20.730270273178764
44	18.669457462778922	23.93340830459136	38.56464118720036	18.832493045429356
45	15.754725610576328	23.230149207152042	38.36017411984999	22.654951062421645
46	19.863975593089016	25.187652341416957	35.26088383580218	19.687488229691848
47	16.03721300625777	21.632077654439893	43.71183057213114	18.6188787671712
48	18.605426986424465	25.253835102690896	35.646142836388684	20.49459507449596
49	15.823598727999613	20.45854430209471	40.389778798917405	23.328078170988277
50	20.057143164612132	19.55566077837384	37.43415353324473	22.953042523769295
51	18.350381223466364	20.477376795140138	36.99239705352195	24.179844927871553
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	25.0
1	149.5
2	274.0
3	8824.0
4	17374.0
5	12049.0
6	6724.0
7	6560.0
8	6396.0
9	6259.5
10	6123.0
11	5737.5
12	5352.0
13	4887.0
14	4422.0
15	4022.5
16	3623.0
17	3294.5
18	2966.0
19	2830.5
20	2695.0
21	2404.5
22	2114.0
23	2044.0
24	1974.0
25	1907.0
26	1855.0
27	1870.0
28	2030.0
29	2190.0
30	2343.0
31	2496.0
32	2668.0
33	2840.0
34	3061.5
35	3283.0
36	3610.0
37	3937.0
38	4065.0
39	4193.0
40	4839.0
41	5485.0
42	6000.0
43	6515.0
44	7549.0
45	8583.0
46	15679.5
47	22776.0
48	18469.0
49	14162.0
50	12977.0
51	11792.0
52	9803.0
53	7814.0
54	6790.0
55	5766.0
56	5364.5
57	4963.0
58	4578.5
59	4194.0
60	3739.5
61	3285.0
62	2972.5
63	2660.0
64	2179.5
65	1699.0
66	1399.0
67	1099.0
68	881.5
69	664.0
70	634.0
71	604.0
72	503.5
73	403.0
74	355.5
75	238.5
76	169.0
77	135.0
78	101.0
79	70.5
80	40.0
81	37.0
82	34.0
83	23.0
84	12.0
85	9.0
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	185849.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.579222917529826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.08891940986221	40.363951379883666
2	6.1937884896548665	6.017788634859483
3	2.80337601346861	4.085574848398431
4	1.6747153426963801	3.2542547982501926
5	1.3202782331310088	3.206904530021684
6	1.0068229143591334	2.934640487707763
7	0.729918922511187	2.482122583387589
8	0.5925745425546055	2.302944863841075
9	0.49621195339152013	2.1695031988334614
>10	2.0047849009791325	14.609710033414222
>50	0.04430463869567144	1.4990664464161765
>100	0.036551326923928935	3.4049147426136273
>500	0.001107615967391786	0.2701117573944439
>1k	0.004430463869567144	4.486437914651142
>5k	0.001107615967391786	2.703807930093786
>10k+	0.001107615967391786	6.2082658502332535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11538	6.2082658502332535	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCG	5025	2.703807930093786	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGC	4032	2.1695031988334614	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTC	2016	1.0847515994167307	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAACGACGT	1173	0.631157552636818	No Hit
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	1117	0.6010255637641311	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAACGACGTCGTA	502	0.2701117573944439	No Hit
GAACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCT	500	0.26903561493470507	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTC	460	0.24751276573992867	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCT	408	0.21953306178671933	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAAACGACGTCGTATGCCG	384	0.20661935226985345	No Hit
CTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGCT	313	0.16841629494912538	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAACGACGTCGT	211	0.11353302950244554	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	204	0.10976653089335967	No Hit
GAATCTTTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTC	200	0.10761424597388201	No Hit
CCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	193	0.10384774736479614	No Hit
TCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	190	0.10223353367518793	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21684270563737226	0.0
2	0.0	0.0	0.0	1.188599346781527	0.0
3	0.0	0.0	0.0	1.4022136250396828	0.0
4	0.0	0.0	0.0	2.0037772600336834	0.0
5	0.0	0.0	0.0	3.5814021060107937	0.0
6	0.0	0.0	0.0	3.864965644151973	0.0
7	0.0	0.0	0.0	4.2954226280475005	0.0
8	0.0	0.0	0.0	4.801209584124747	0.0
9	0.0	0.0	0.0	4.936803534051838	0.0
10	0.0	0.0	0.0	8.827058526007672	0.0
11	0.0	0.0	0.0	9.463596790943186	0.0
12	0.0	0.0	0.0	12.545668795635166	0.0
13	0.0	0.0	0.0	12.813090196880262	0.0
14	0.0	0.0	0.0	12.978278064450173	0.0
15	0.0	0.0	0.0	13.482988878067678	0.0
16	0.0	0.0	0.0	13.8273544651841	0.0
17	0.0	0.0	0.0	14.158268271553789	0.0
18	0.0	0.0	0.0	14.46712115749883	0.0
19	0.0	0.0	0.0	15.567476822581773	0.0
20	0.0	0.0	0.0	15.905385554939762	0.0
21	0.0	0.0	0.0	16.23683743253932	0.0
22	0.0	0.0	0.0	16.819568574487892	0.0
23	0.0	0.0	0.0	17.21451285721204	0.0
24	0.0	0.0	0.0	17.44964998466497	0.0
25	0.0	0.0	0.0	17.67833025735947	0.0
26	0.0	0.0	0.0	17.87687854118128	0.0
27	0.0	0.0	0.0	18.07058418393427	0.0
28	0.0	0.0	0.0	18.265365969146995	0.0
29	0.0	0.0	0.0	18.47090917895711	0.0
30	0.0	0.0	0.0	18.697437166732133	0.0
31	0.0	0.0	0.0	18.914279872369505	0.0
32	0.0	0.0	0.0	19.113366227421185	0.0
33	0.0	0.0	0.0	19.34581299872477	0.0
34	0.0	0.0	0.0	19.559965348212796	0.0
35	0.0	0.0	0.0	19.77519384016056	0.0
36	0.0	0.0	0.0	19.963518770614854	0.0
37	0.0	0.0	0.0	20.19542747068857	0.0
38	0.0	0.0	0.0	20.400432609268815	0.0
39	0.0	0.0	0.0	20.645255018859398	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCACCG	175	0.0	45.000004	14
CTTAATG	20	7.0180104E-4	45.0	45
TCTTGCG	40	6.7611836E-9	45.0	1
CTCGTTG	25	3.8781487E-5	45.0	1
CACGGGA	20	7.0180104E-4	45.0	4
ACCGCTG	20	7.0180104E-4	45.0	17
TCGGGCA	20	7.0180104E-4	45.0	5
TTATACG	25	3.8781487E-5	45.0	26
GCACACA	20	7.0180104E-4	45.0	9
CGGTGAC	20	7.0180104E-4	45.0	19
CCGGTGA	25	3.8781487E-5	45.0	18
ACCCGTC	20	7.0180104E-4	45.0	17
GAACATG	25	3.8781487E-5	45.0	9
ATACGGA	20	7.0180104E-4	45.0	28
GGATTAG	20	7.0180104E-4	45.0	8
GGCGACT	100	0.0	45.0	10
CCGCCTC	35	1.2045894E-7	45.0	15
CACCCGT	25	3.8781487E-5	45.0	16
GCGACCT	65	0.0	44.999996	11
CCTCGGG	30	2.1556534E-6	44.999996	3
>>END_MODULE