##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933376.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411962
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.192156072647478	31.0	28.0	31.0	25.0	34.0
2	28.27119248862759	31.0	26.0	33.0	16.0	34.0
3	28.300224292531837	31.0	25.0	33.0	16.0	34.0
4	32.89148999179536	35.0	32.0	37.0	28.0	37.0
5	34.114287240085254	35.0	33.0	37.0	30.0	37.0
6	33.34238109340182	35.0	32.0	37.0	28.0	37.0
7	33.39270369597196	35.0	32.0	37.0	28.0	37.0
8	33.18295619498886	35.0	32.0	37.0	28.0	37.0
9	34.466577014384825	37.0	34.0	39.0	28.0	39.0
10	34.347745665862384	37.0	32.0	39.0	27.0	39.0
11	34.55968268918007	37.0	33.0	39.0	27.0	39.0
12	34.656215864570036	37.0	33.0	39.0	27.0	39.0
13	34.77717605021822	37.0	33.0	39.0	27.0	39.0
14	35.554716211689424	38.0	34.0	40.0	27.0	41.0
15	35.78574722911336	38.0	34.0	40.0	27.0	41.0
16	35.78704346517397	38.0	34.0	40.0	27.0	41.0
17	35.02173501439453	37.0	33.0	40.0	27.0	41.0
18	34.85403993572223	37.0	33.0	39.0	27.0	40.0
19	34.201071943528774	37.0	32.0	38.0	27.0	40.0
20	33.60119622683646	35.0	32.0	38.0	25.0	40.0
21	34.11504216408309	35.0	33.0	38.0	27.0	40.0
22	34.718770177831935	35.0	33.0	39.0	28.0	40.0
23	35.041406731688845	35.0	33.0	39.0	29.0	40.0
24	34.583796563760735	35.0	33.0	39.0	27.0	40.0
25	33.602145343502556	35.0	32.0	38.0	24.0	40.0
26	33.70706521475282	35.0	32.0	38.0	24.0	40.0
27	34.318301202538095	35.0	33.0	39.0	26.0	40.0
28	34.050172103252244	35.0	33.0	39.0	25.0	40.0
29	34.105856850874595	35.0	33.0	39.0	24.0	40.0
30	33.5225457687845	35.0	33.0	39.0	22.0	40.0
31	33.489428636621824	35.0	33.0	39.0	22.0	40.0
32	32.8447987921216	35.0	31.0	39.0	18.0	40.0
33	32.38130215893699	35.0	31.0	39.0	15.0	40.0
34	32.112012758458306	35.0	31.0	39.0	12.0	40.0
35	31.731608740612	35.0	30.0	40.0	10.0	41.0
36	31.145057068370384	35.0	29.0	39.0	8.0	40.0
37	30.949500196620075	35.0	28.0	39.0	8.0	40.0
38	30.755822138935144	35.0	27.0	39.0	8.0	40.0
39	30.41276865341949	35.0	25.0	39.0	8.0	40.0
40	30.251319296439963	35.0	24.0	39.0	8.0	40.0
41	29.809205703438668	35.0	23.0	39.0	7.0	40.0
42	30.047283487311937	35.0	23.0	39.0	7.0	40.0
43	29.889960724532845	35.0	23.0	39.0	7.0	40.0
44	30.081473533966726	35.0	23.0	39.0	7.0	40.0
45	30.146239216238392	35.0	23.0	39.0	7.0	40.0
46	29.887385244270103	35.0	23.0	39.0	7.0	40.0
47	29.832943329724586	35.0	23.0	39.0	7.0	40.0
48	29.68612396288978	35.0	23.0	38.0	7.0	40.0
49	29.8518164296707	35.0	24.0	39.0	7.0	40.0
50	29.768405823838123	35.0	24.0	39.0	7.0	40.0
51	28.702666750816825	34.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	24.0
10	34.0
11	32.0
12	41.0
13	59.0
14	71.0
15	128.0
16	208.0
17	481.0
18	872.0
19	1747.0
20	2460.0
21	3620.0
22	5080.0
23	7099.0
24	9936.0
25	14241.0
26	18050.0
27	19798.0
28	19245.0
29	18508.0
30	19629.0
31	22387.0
32	26086.0
33	29788.0
34	34298.0
35	38811.0
36	42041.0
37	42003.0
38	30926.0
39	4252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.576849321053885	2.625727615653871	31.492225011044706	35.30519805224754
2	34.08566809560105	19.271923138541904	32.28768672838757	14.354722037469475
3	12.824483811613693	19.54160820658216	53.68140750845952	13.952500473344628
4	11.510770410863138	2.9934799811633113	69.06947728188523	16.426272326088327
5	24.493763987940635	3.518042926289318	54.013719712012275	17.974473373757775
6	13.967064923463814	17.257659687058517	57.19993591641948	11.575339473058195
7	53.3532218991072	1.3884775780290415	42.28812366189115	2.970176860972614
8	52.010865079788914	13.35657172263461	31.117918642981635	3.514644554594841
9	49.098946019293045	2.9090061704720336	29.90469994805346	18.087347862181463
10	30.90916152460664	18.65293400847651	36.606531670396784	13.831372796520066
11	26.810482520232448	15.746597987192992	42.98673178594142	14.456187706633136
12	19.95014103242532	14.464198154198687	45.73334433758453	19.852316475791458
13	18.779644724513425	15.465018618222068	53.18378879605401	12.5715478612105
14	15.71091508440099	21.7898738233138	45.22917162262539	17.270039469659825
15	12.38803578970876	14.694316466081823	55.312868662643645	17.604779081565773
16	14.972740204193592	17.453551541161563	43.71786718192455	23.8558410727203
17	15.481767735859133	18.183958714638727	49.45310489802458	16.881168651477562
18	16.321651026065513	16.08959078749982	47.19634335205674	20.392414834377927
19	15.592457556764943	19.281875512790016	43.812050626028615	21.313616304416428
20	18.43859385088916	18.739349745850344	49.585884134944486	13.23617226831601
21	17.934906617600653	24.308795471426976	44.116933115190236	13.639364795782136
22	15.982784819959123	14.718833290449119	47.917040892121115	21.38134099747064
23	16.416562692675537	23.094120331486884	46.01589466989674	14.473422305940838
24	17.340434311902552	17.94874284521388	43.76714357149446	20.943679271389108
25	15.134405600516551	27.251542618008457	41.236813104121254	16.377238677353738
26	15.343648200562187	16.02769187449328	46.94365014248887	21.685009782455662
27	18.73570863332055	18.89785951131415	41.73855841072721	20.627873444638098
28	13.043921526742757	20.405522839485197	47.43520033401139	19.115355299760658
29	19.120938338973012	16.31194139265272	42.9066273102859	21.66049295808837
30	19.16511717100121	20.987615362581984	45.03206606434574	14.815201402071065
31	22.437749112782246	15.983755783300403	40.998198863001925	20.580296240915423
32	22.289191721566553	19.904020273714565	42.53134997888155	15.275438025837335
33	18.482287201246717	16.851068787897912	41.02587131822838	23.640772692626992
34	20.138750661468777	19.755948364169512	40.91154038479277	19.19376058956894
35	23.616741349930333	21.05194168394172	37.13206557886407	18.199251387263875
36	22.36371315800972	21.162874245682854	36.10721377214403	20.36619882416339
37	21.948869070448247	21.232298124584307	40.01801136998073	16.80082143498672
38	23.666260480335566	21.85152999548502	35.745772668352906	18.736436855826508
39	21.132288900432563	20.538544817240425	39.825517887572154	18.50364839475486
40	24.42652477655706	20.888334360936202	37.03958132060724	17.645559541899495
41	19.673173739325474	22.788024138148664	38.203280885130184	19.33552123739568
42	22.609124142518	21.108985780241866	38.15813108976071	18.123758987479427
43	21.38935144503619	19.99334890111224	35.61396439477428	23.003335259077293
44	23.769910817017102	20.893674659313238	34.27864705968026	21.057767463989396
45	20.815512110340272	19.645986765769656	34.57576184211165	24.96273928177842
46	24.24568285424384	23.16815628625941	32.754477354707475	19.831683504789275
47	16.82485277768338	20.945621198071667	43.113199761143015	19.11632626310194
48	21.321626751981977	24.041780552575236	32.37021861239629	22.266374083046493
49	20.105495167029968	20.202591501157873	38.002291473485414	21.68962185832674
50	19.966161927556424	20.209630985382145	36.39583262533923	23.4283744617222
51	20.23827440394988	20.39047290769537	33.13024016778247	26.241012520572287
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30.0
1	512.5
2	995.0
3	11830.5
4	22666.0
5	16848.5
6	11031.0
7	10502.5
8	9974.0
9	9605.5
10	9237.0
11	8842.5
12	8448.0
13	7897.5
14	7347.0
15	6855.0
16	6363.0
17	5765.5
18	5168.0
19	4788.0
20	4408.0
21	4100.0
22	3792.0
23	3782.0
24	3772.0
25	3809.0
26	4205.5
27	4565.0
28	4733.5
29	4902.0
30	5378.0
31	5854.0
32	6476.5
33	7099.0
34	7909.5
35	8720.0
36	9199.5
37	9679.0
38	10235.0
39	10791.0
40	10957.0
41	11123.0
42	12165.5
43	13208.0
44	14453.0
45	15698.0
46	41227.5
47	66757.0
48	46591.0
49	26425.0
50	24842.0
51	23259.0
52	21068.0
53	18877.0
54	18285.0
55	17693.0
56	17210.5
57	16728.0
58	15604.0
59	14480.0
60	12982.0
61	11484.0
62	9939.5
63	8395.0
64	7282.5
65	6170.0
66	5180.0
67	4190.0
68	3590.5
69	2991.0
70	2505.5
71	2020.0
72	1632.5
73	1245.0
74	1153.0
75	904.0
76	747.0
77	575.5
78	404.0
79	298.0
80	192.0
81	134.5
82	77.0
83	51.5
84	26.0
85	18.0
86	10.0
87	9.0
88	8.0
89	6.0
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	411962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.0982619214942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.5507968593325	35.96248399809411
2	5.883973283044502	5.189459899495411
3	2.6551043756948514	3.5125646456489066
4	1.940215857770638	3.422405883208245
5	1.5083211106283998	3.3257169699105096
6	1.2136786160212538	3.21126704986931
7	1.0618695118290278	3.2778619901359956
8	0.8482006230221292	2.992333858880353
9	0.6731929959496378	2.6717976953192255
>10	2.5639639704397945	16.379577959400812
>50	0.0572946299200641	1.6996861197077509
>100	0.03615680528936083	2.783423062537718
>500	0.0016687756287397306	0.4837322886511307
>1k	0.003337551257479461	3.4582443232269986
>5k	0.0	0.0
>10k+	0.0022250341716529743	11.629444255913517
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCG	14299	3.470951204237284	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTC	11238	2.727921507323491	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGC	11147	2.7058320913093925	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10725	2.603395458804453	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGTTTGTT	2702	0.6558857370339983	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTC	2409	0.5847626722853079	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCT	2406	0.5840344497793486	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCT	2256	0.5476233244813843	No Hit
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	2202	0.5345153193741171	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTA	2123	0.5153387933838558	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGT	811	0.1968628174443274	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTT	629	0.15268398541613062	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTAT	532	0.12913812439011366	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATG	454	0.11020433923517217	No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	422	0.10243663250493978	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.427408353197625E-4	0.0	0.0	0.08326010651467854	0.0
2	2.427408353197625E-4	0.0	0.0	0.7100169433103053	0.0
3	2.427408353197625E-4	0.0	0.0	0.8209495050514368	0.0
4	2.427408353197625E-4	0.0	0.0	1.530966448361742	0.0
5	2.427408353197625E-4	0.0	0.0	4.730533398711532	0.0
6	2.427408353197625E-4	0.0	0.0	5.000703948422427	0.0
7	2.427408353197625E-4	0.0	0.0	5.7044096300144185	0.0
8	2.427408353197625E-4	0.0	0.0	6.45981910952952	0.0
9	2.427408353197625E-4	0.0	0.0	6.592841087284749	0.0
10	2.427408353197625E-4	0.0	0.0	10.712395803496438	0.0
11	2.427408353197625E-4	0.0	0.0	10.913142474305882	0.0
12	2.427408353197625E-4	0.0	0.0	14.228739543938518	0.0
13	2.427408353197625E-4	0.0	0.0	14.479733567659153	0.0
14	2.427408353197625E-4	0.0	0.0	14.682422165151154	0.0
15	2.427408353197625E-4	0.0	0.0	15.394138294308698	0.0
16	4.85481670639525E-4	0.0	0.0	15.726935979532092	0.0
17	4.85481670639525E-4	0.0	0.0	15.894184415067409	0.0
18	4.85481670639525E-4	0.0	0.0	16.023565280292843	0.0
19	4.85481670639525E-4	0.0	0.0	16.93627082109515	0.0
20	4.85481670639525E-4	0.0	0.0	17.135318306057354	0.0
21	4.85481670639525E-4	0.0	0.0	17.274651545530897	0.0
22	4.85481670639525E-4	0.0	0.0	17.547492244430313	0.0
23	4.85481670639525E-4	0.0	0.0	17.683184371374058	0.0
24	4.85481670639525E-4	0.0	0.0	17.807710419893098	0.0
25	4.85481670639525E-4	0.0	0.0	17.904806754021003	0.0
26	4.85481670639525E-4	0.0	0.0	18.038071472611552	0.0
27	4.85481670639525E-4	0.0	0.0	18.24197377428015	0.0
28	4.85481670639525E-4	0.0	0.0	18.35509100353916	0.0
29	4.85481670639525E-4	0.0	0.0	18.492967798000787	0.0
30	4.85481670639525E-4	0.0	0.0	18.640068744204562	0.0
31	4.85481670639525E-4	0.0	0.0	18.75731256766401	0.0
32	4.85481670639525E-4	0.0	0.0	18.904656254703102	0.0
33	4.85481670639525E-4	0.0	0.0	19.034279860763856	0.0
34	4.85481670639525E-4	0.0	0.0	19.236725717420537	0.0
35	4.85481670639525E-4	0.0	0.0	19.40858622882693	0.0
36	4.85481670639525E-4	0.0	0.0	19.557871842548586	0.0
37	4.85481670639525E-4	0.0	0.0	19.71589612634175	0.0
38	4.85481670639525E-4	0.0	0.0	19.900621902020088	0.0
39	4.85481670639525E-4	0.0	0.0	20.180744825979094	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATG	20	7.02801E-4	45.000004	1
GCGATTC	40	6.7975634E-9	45.000004	9
CCGACAG	20	7.02801E-4	45.000004	43
CTCACCA	25	3.8864335E-5	45.0	31
ACGGGAG	50	2.1827873E-11	45.0	5
GCGGGTA	45	3.8380676E-10	45.0	5
ATCGAGT	25	3.8864335E-5	45.0	42
AATCGAG	25	3.8864335E-5	45.0	41
CATATGC	60	0.0	44.999996	33
CGTTTTT	9205	0.0	44.193375	1
ACGGCTG	1415	0.0	42.61484	8
TACGGCT	1410	0.0	42.606384	7
GAATCTG	1275	0.0	42.352943	1
CGGCTGT	1435	0.0	42.33449	9
CCGATGA	420	0.0	42.32143	18
CGATGAA	415	0.0	42.289158	19
TGATACC	1840	0.0	42.187496	4
GATACCT	1845	0.0	41.95122	5
GAATGAC	340	0.0	41.691177	1
GCCAGTA	65	0.0	41.538464	25
>>END_MODULE