FastQCFastQC Report
Sat 14 Jan 2017
SRR2933374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343475
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG179005.211441880777349No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC159384.64022126792343No Hit
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC132133.846859305626319No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106453.0992066380377032No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTAGCACT36381.0591746124172063No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC32080.933983550476745No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTA30670.8929325278404543No Hit
GAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT28520.8303369968702234No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT28460.8285901448431473No Hit
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC26840.7814251401120896TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTAGCACTCGT11410.33219302714899196No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTAT8060.23466045563723703No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATG7040.20496397117694154No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT6760.19681199505058594No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCACTAGCACT4450.12955819200815197No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACTAGCAC4340.12635562995851227No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3840.1117985297328772No Hit
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT3590.10451997962005968TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATG207.026371E-445.0000041
TTGATAC1300.045.0000043
CGGGTAC253.885074E-545.0000046
GGTACGG406.7902874E-945.0000045
CGACGAA253.885074E-545.00000419
ATTGTTG207.026371E-445.0000041
TACGGGA253.885074E-545.0000044
CCTGTAC253.885074E-545.00000415
ACGATAC253.885074E-545.0000043
CTACGGG207.026371E-445.0000043
GCCTCGG253.885074E-545.0000042
AACGATA253.885074E-545.0000042
AACGAGG253.885074E-545.00000428
TAAGGCG207.026371E-445.00000429
GACGAAT253.885074E-545.00000420
CGCCGAT207.026371E-445.00000416
ACATACG502.1827873E-1145.00000417
ATCATAC253.885074E-545.00000425
CGATACG207.026371E-445.0000044
ACCGACG351.2086093E-745.017
Produced by FastQC (version 0.11.5)