SRR2933373.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933373.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	226146
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16876	7.462435771581191	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG	8001	3.5379798890981933	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC	6308	2.789348473994676	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC	3665	1.6206344573859364	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTAGCACT	1676	0.7411141474976343	No Hit
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	1500	0.6632883181661404	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	1186	0.5244399635633618	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTA	1027	0.4541314018377508	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC	981	0.43379056008065586	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCACTAGCACTCGTATGCCG	690	0.3051126263564246	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT	641	0.2834452079629973	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTAGCACTCGT	407	0.17997223032907944	No Hit
GAATCTTTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC	388	0.17157057829897499	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCACTAGCACTCGTATGC	379	0.16759084838997818	No Hit
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT	345	0.1525563131782123	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGACTCTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT	329	0.14548123778444014	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTAT	296	0.13088889478478505	No Hit
GAATCTGTCTCTTATCCACATCTGACGCACTAGCACTCGTATGCCGTCTTC	274	0.12116066611834832	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC	250	0.11054805302769008	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	247	0.1092214763913578	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATG	245	0.10833709196713627	No Hit
GAATGATCCGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC	241	0.10656832311869324	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT	236	0.10435736205813943	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	236	0.10435736205813943	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT	229	0.10126201657336412	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGGTAC	30	2.1577434E-6	45.000004	6
AATGCTC	35	1.2061537E-7	45.000004	2
TTCGGGC	35	1.2061537E-7	45.000004	4
CTTGTTG	25	3.880838E-5	45.0	1
TCGTTTG	50	2.1827873E-11	45.0	1
TCGTTGA	25	3.880838E-5	45.0	24
ACCGGTC	20	7.021256E-4	45.0	17
CCGATGA	95	0.0	45.0	18
TCCTGAT	20	7.021256E-4	45.0	16
CGTTGAT	25	3.880838E-5	45.0	25
GAGTAAC	25	3.880838E-5	45.0	9
TAGCGGG	20	7.021256E-4	45.0	3
ACCTGTA	25	3.880838E-5	45.0	14
ACCGACT	20	7.021256E-4	45.0	17
GATCAAC	25	3.880838E-5	45.0	9
CCTGTAC	25	3.880838E-5	45.0	15
TGCGGGC	50	2.1827873E-11	45.0	4
CCCCGTG	20	7.021256E-4	45.0	17
CTACGGC	105	0.0	45.0	6
TGTTCCG	20	7.021256E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content