##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933373.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 226146 Sequences flagged as poor quality 0 Sequence length 51 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.842840465893715 31.0 25.0 31.0 16.0 33.0 2 27.662598498315248 31.0 26.0 33.0 16.0 34.0 3 27.810498527499934 31.0 25.0 33.0 16.0 34.0 4 31.271581190912066 35.0 28.0 35.0 19.0 37.0 5 33.47661245390146 35.0 33.0 37.0 28.0 37.0 6 31.623110733773757 35.0 32.0 37.0 17.0 37.0 7 32.12747074898517 35.0 32.0 35.0 25.0 37.0 8 32.13134877468538 35.0 32.0 35.0 17.0 37.0 9 32.96864415023923 35.0 32.0 37.0 25.0 39.0 10 31.773632962776258 35.0 28.0 39.0 15.0 39.0 11 32.522083079072814 35.0 29.0 39.0 17.0 39.0 12 33.328964474277676 35.0 31.0 39.0 24.0 39.0 13 33.47901355761322 35.0 32.0 39.0 25.0 39.0 14 33.68097158472845 36.0 32.0 40.0 21.0 41.0 15 34.00452804825201 36.0 32.0 40.0 24.0 41.0 16 34.27823176178221 36.0 32.0 40.0 25.0 41.0 17 32.13524006615196 35.0 27.0 39.0 18.0 41.0 18 32.80183598206468 36.0 30.0 39.0 22.0 39.0 19 32.60053239942338 36.0 30.0 37.0 21.0 39.0 20 31.856893334394595 34.0 30.0 36.0 22.0 39.0 21 32.51779381461534 35.0 31.0 37.0 23.0 39.0 22 32.75177097980951 34.0 30.0 37.0 25.0 39.0 23 33.533611030042536 35.0 32.0 38.0 26.0 40.0 24 32.18591971558197 35.0 31.0 37.0 21.0 39.0 25 31.456505974016785 34.0 30.0 37.0 18.0 39.0 26 30.502454166777216 33.0 25.0 37.0 18.0 39.0 27 32.290493751824044 34.0 30.0 37.0 22.0 39.0 28 32.0664305360254 35.0 30.0 37.0 21.0 39.0 29 32.16086952676589 35.0 30.0 38.0 21.0 40.0 30 31.389186631645043 34.0 29.0 37.0 18.0 40.0 31 31.709789251191708 35.0 30.0 37.0 20.0 39.0 32 31.569150018129882 35.0 30.0 37.0 18.0 39.0 33 31.482259248450116 35.0 30.0 38.0 15.0 40.0 34 30.627846612365463 35.0 28.0 38.0 12.0 40.0 35 30.138468069300362 35.0 26.0 38.0 10.0 40.0 36 29.570206857516826 35.0 24.0 38.0 8.0 40.0 37 29.53178477620652 35.0 23.0 38.0 8.0 40.0 38 29.34860665233964 35.0 23.0 38.0 8.0 40.0 39 28.920007428829162 34.0 22.0 37.0 7.0 40.0 40 28.580708922554457 33.0 22.0 37.0 7.0 40.0 41 28.287115403323515 33.0 21.0 37.0 7.0 40.0 42 28.61191442696311 33.0 21.0 37.0 7.0 40.0 43 28.634643106665607 34.0 21.0 38.0 7.0 40.0 44 28.651972619458228 34.0 21.0 38.0 7.0 40.0 45 28.79466804630637 34.0 20.0 38.0 7.0 40.0 46 28.42756891565626 34.0 20.0 38.0 7.0 40.0 47 28.44178981719774 34.0 20.0 38.0 7.0 40.0 48 28.34054548831286 34.0 20.0 37.0 7.0 40.0 49 28.192698522193627 34.0 20.0 37.0 7.0 40.0 50 28.350990068362915 34.0 20.0 37.0 7.0 40.0 51 26.715679251457022 32.0 18.0 35.0 7.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 5.0 10 13.0 11 15.0 12 17.0 13 31.0 14 49.0 15 130.0 16 257.0 17 517.0 18 919.0 19 1494.0 20 2181.0 21 2951.0 22 4088.0 23 5264.0 24 7060.0 25 9658.0 26 12315.0 27 13469.0 28 13556.0 29 13748.0 30 15064.0 31 17200.0 32 19055.0 33 20645.0 34 21364.0 35 21957.0 36 14358.0 37 7182.0 38 1557.0 39 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.606298585869304 2.217593943735463 28.145092108637783 41.03101536175745 2 40.6591317113723 18.9612020553094 28.56252155687034 11.81714467644796 3 10.753672406321579 18.98286947370283 58.524139272859124 11.739318847116465 4 10.221715175152335 2.636349968604353 73.63650031395646 13.50543454228684 5 23.23631636199623 2.983028662899189 58.360528154378144 15.420126820726434 6 11.8640170509317 16.793133639330343 60.90401775843923 10.438831551298719 7 44.247079320439006 1.2677650721215497 51.524236555145784 2.9609190522936513 8 43.93223846541615 13.767212331856413 37.64028547929214 4.660263723435302 9 40.569366692313814 2.6283905087863593 37.37718111308624 19.42506168581359 10 27.895695701007313 15.08627170058281 42.77634802295862 14.241684575451258 11 23.06474578369726 14.869155324436425 49.897411406790305 12.168687485076013 12 18.86170880758448 11.670336862027186 50.53991669098724 18.928037639401094 13 16.12630778346732 14.169165052665091 58.74125564900551 10.96327151486208 14 14.200560699724956 19.05848434197377 48.48549167352065 18.255463284780628 15 9.517302981259894 14.475162063445737 60.43440962917761 15.573125326116758 16 10.953985478407754 13.9763692481848 49.59141439600966 25.478230877397788 17 11.6093143367559 16.351825811643806 55.63971947326064 16.39914037833966 18 11.162700202524032 14.7612604246814 53.61049941188436 20.46553996091021 19 12.234131932468404 18.076817631087884 49.881934679366424 19.807115757077288 20 15.118993924279007 15.590813014601187 57.41688997373379 11.873303087386025 21 15.57002998063198 22.37846347050136 48.30242409770679 13.74908245115987 22 12.598940507459783 13.993614744457119 52.938367249476 20.469077498607096 23 13.454140245681993 22.563299815163656 49.41055778125636 14.572002157897995 24 14.950518691464806 15.025249175311526 49.11605776799059 20.90817436523308 25 12.21953958946875 23.662589654471006 47.10496758731085 17.01290316874939 26 11.940516303626861 16.33634908421993 52.94102040274866 18.782114209404543 27 13.632785899374742 18.810414510979633 48.08177018386352 19.475029405782106 28 10.336242958089022 18.85463373219071 55.51546346165751 15.293659848062758 29 15.842420383292211 17.368867899498554 48.45321164203656 18.335500075172675 30 13.451487092409328 21.42155952349367 49.68560133718925 15.441352046907749 31 15.871162877079408 16.71972973211996 46.87193229152848 20.53717509927215 32 18.02994525660414 24.5376880422382 43.60590061287841 13.826466088279254 33 16.090048022074235 16.38941214967322 42.599028945902205 24.92151088235034 34 16.878476736267718 24.484624976784907 41.35602663765886 17.280871649288514 35 13.25382717359582 21.939808796087483 40.00070750753938 24.80565652277732 36 14.708197359228109 22.2856031059581 42.25854094257692 20.747658592236874 37 13.902523148762306 24.76453264705102 41.5262706393215 19.806673564865175 38 20.458464885516435 20.783033969205732 40.616239066797554 18.14226207848027 39 15.946335553138239 24.924606227835117 38.30401598967039 20.825042229356256 40 18.708268109982047 24.129986822672077 39.47892069724868 17.682824370097194 41 15.104401581279351 21.046138335411637 37.934343300345795 25.91511678296322 42 14.63302468316928 24.34577662218213 42.48494335517763 18.536255339470962 43 17.434754539103057 20.59642885569499 36.62191681480106 25.34689979040089 44 18.68129438504329 20.126378534221256 39.09067593501543 22.101651145720023 45 15.18001644955029 20.57166609181679 40.265580642593726 23.982736816039196 46 19.892016661802554 25.725858516179812 35.673414519823474 18.708710302194156 47 15.520946645087685 19.238014380090736 46.36915974635855 18.87187922846303 48 18.364684761171986 21.53166538430925 36.78685450991837 23.31679534460039 49 14.68564555641046 20.21835451434029 43.174763206070416 21.92123672317883 50 18.125458774420064 17.73323428227782 39.371467989705764 24.76983895359635 51 17.517444482767768 18.764868713132223 37.69821265907865 26.01947414502136 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 48.0 1 243.0 2 438.0 3 12100.5 4 23763.0 5 16060.5 6 8358.0 7 7983.0 8 7608.0 9 7351.0 10 7094.0 11 6607.0 12 6120.0 13 5471.5 14 4823.0 15 4367.5 16 3912.0 17 3466.5 18 3021.0 19 2760.5 20 2500.0 21 2321.0 22 2142.0 23 2034.5 24 1927.0 25 1899.0 26 1823.0 27 1775.0 28 2038.0 29 2301.0 30 2452.0 31 2603.0 32 2703.0 33 2803.0 34 3054.0 35 3305.0 36 3568.0 37 3831.0 38 4072.5 39 4314.0 40 5010.5 41 5707.0 42 6315.5 43 6924.0 44 8001.5 45 9079.0 46 21201.5 47 33324.0 48 25816.5 49 18309.0 50 16380.0 51 14451.0 52 11533.5 53 8616.0 54 7664.5 55 6713.0 56 6194.0 57 5675.0 58 5448.5 59 5222.0 60 4897.5 61 4573.0 62 4139.0 63 3705.0 64 3231.5 65 2758.0 66 2414.0 67 2070.0 68 1820.0 69 1570.0 70 1320.5 71 1071.0 72 871.0 73 671.0 74 560.5 75 374.5 76 299.0 77 242.0 78 185.0 79 140.5 80 96.0 81 67.5 82 39.0 83 43.0 84 47.0 85 31.0 86 15.0 87 13.0 88 11.0 89 8.5 90 6.0 91 4.5 92 3.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 226146.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.27558303043167 #Duplication Level Percentage of deduplicated Percentage of total 1 82.41166492959762 35.664128483369154 2 6.301473443279586 5.453998744174117 3 2.6607810679909263 3.454405561009259 4 1.7707886293503363 3.0652764143517905 5 1.3487830298571515 2.918468599931018 6 0.9911511658798765 2.573558674484625 7 0.7857682954243557 2.3803206777922226 8 0.6743915149285757 2.3347748799448143 9 0.5466658492224061 2.1291555013133108 >10 2.3910244620194963 15.582411362571083 >50 0.0623301248646108 1.8107771085935636 >100 0.04393762900292236 3.8170031749400826 >500 0.003065415976948072 1.022348394400078 >1k 0.005109026628246786 4.003608288450824 >5k 0.0020436106512987146 6.32732836309287 >10k+ 0.0010218053256493573 7.462435771581191 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16876 7.462435771581191 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG 8001 3.5379798890981933 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC 6308 2.789348473994676 No Hit GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC 3665 1.6206344573859364 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTAGCACT 1676 0.7411141474976343 No Hit GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC 1500 0.6632883181661404 TruSeq Adapter, Index 16 (95% over 21bp) GAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT 1186 0.5244399635633618 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTA 1027 0.4541314018377508 No Hit GAATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC 981 0.43379056008065586 No Hit GAATGATACCTGTCTCTTCTACACATCTGACGCACTAGCACTCGTATGCCG 690 0.3051126263564246 No Hit GAATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT 641 0.2834452079629973 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTAGCACTCGT 407 0.17997223032907944 No Hit GAATCTTTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC 388 0.17157057829897499 No Hit GAATGATACGGCTGCCTCCTATACACATCTGACGCACTAGCACTCGTATGC 379 0.16759084838997818 No Hit CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT 345 0.1525563131782123 TruSeq Adapter, Index 13 (95% over 22bp) GAATGACTCTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT 329 0.14548123778444014 No Hit GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTAT 296 0.13088889478478505 No Hit GAATCTGTCTCTTATCCACATCTGACGCACTAGCACTCGTATGCCGTCTTC 274 0.12116066611834832 No Hit GAATGCTACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC 250 0.11054805302769008 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 247 0.1092214763913578 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATG 245 0.10833709196713627 No Hit GAATGATCCGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC 241 0.10656832311869324 No Hit GAATGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT 236 0.10435736205813943 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 236 0.10435736205813943 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT 229 0.10126201657336412 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.421922121107603E-4 0.0 0.0 0.18174099917752248 0.0 2 4.421922121107603E-4 0.0 0.0 1.1647342866997425 0.0 3 4.421922121107603E-4 0.0 0.0 1.391136699300452 0.0 4 4.421922121107603E-4 0.0 0.0 2.2808274300673017 0.0 5 4.421922121107603E-4 0.0 0.0 4.40865635474428 0.0 6 4.421922121107603E-4 0.0 0.0 4.755777241251227 0.0 7 4.421922121107603E-4 0.0 0.0 5.2696045917239305 0.0 8 4.421922121107603E-4 0.0 0.0 5.973574593404261 0.0 9 4.421922121107603E-4 0.0 0.0 6.167696974520885 0.0 10 4.421922121107603E-4 0.0 0.0 10.75367240632158 0.0 11 4.421922121107603E-4 0.0 0.0 11.374510272125088 0.0 12 4.421922121107603E-4 0.0 0.0 15.04647440149284 0.0 13 4.421922121107603E-4 0.0 0.0 15.35335579669771 0.0 14 4.421922121107603E-4 0.0 0.0 15.559417367541323 0.0 15 4.421922121107603E-4 0.0 0.0 16.25498571719155 0.0 16 4.421922121107603E-4 0.0 0.0 16.647652401545905 0.0 17 4.421922121107603E-4 0.0 0.0 16.938614877114784 0.0 18 4.421922121107603E-4 0.0 0.0 17.201719243320685 0.0 19 4.421922121107603E-4 0.0 0.0 18.370433259929428 0.0 20 4.421922121107603E-4 0.0 0.0 18.72020729970904 0.0 21 4.421922121107603E-4 0.0 0.0 19.010285390853696 0.0 22 4.421922121107603E-4 0.0 0.0 19.54135823759872 0.0 23 4.421922121107603E-4 0.0 0.0 19.836300443076595 0.0 24 4.421922121107603E-4 0.0 0.0 20.08835000397973 0.0 25 4.421922121107603E-4 0.0 0.0 20.290874037126457 0.0 26 4.421922121107603E-4 0.0 0.0 20.469961883031317 0.0 27 4.421922121107603E-4 0.0 0.0 20.656124804329945 0.0 28 4.421922121107603E-4 0.0 0.0 20.80823892529605 0.0 29 4.421922121107603E-4 0.0 0.0 20.988211155625127 0.0 30 4.421922121107603E-4 0.0 0.0 21.173931884711646 0.0 31 4.421922121107603E-4 0.0 0.0 21.353019730616506 0.0 32 4.421922121107603E-4 0.0 0.0 21.527243462188142 0.0 33 4.421922121107603E-4 0.0 0.0 21.696160887214454 0.0 34 4.421922121107603E-4 0.0 0.0 21.86817365772554 0.0 35 4.421922121107603E-4 0.0 0.0 22.061853846630054 0.0 36 4.421922121107603E-4 0.0 0.0 22.255091843322454 0.0 37 4.421922121107603E-4 0.0 0.0 22.437275034712087 0.0 38 4.421922121107603E-4 0.0 0.0 22.601328345405182 0.0 39 4.421922121107603E-4 0.0 0.0 22.76980357821938 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGTAC 30 2.1577434E-6 45.000004 6 AATGCTC 35 1.2061537E-7 45.000004 2 TTCGGGC 35 1.2061537E-7 45.000004 4 CTTGTTG 25 3.880838E-5 45.0 1 TCGTTTG 50 2.1827873E-11 45.0 1 TCGTTGA 25 3.880838E-5 45.0 24 ACCGGTC 20 7.021256E-4 45.0 17 CCGATGA 95 0.0 45.0 18 TCCTGAT 20 7.021256E-4 45.0 16 CGTTGAT 25 3.880838E-5 45.0 25 GAGTAAC 25 3.880838E-5 45.0 9 TAGCGGG 20 7.021256E-4 45.0 3 ACCTGTA 25 3.880838E-5 45.0 14 ACCGACT 20 7.021256E-4 45.0 17 GATCAAC 25 3.880838E-5 45.0 9 CCTGTAC 25 3.880838E-5 45.0 15 TGCGGGC 50 2.1827873E-11 45.0 4 CCCCGTG 20 7.021256E-4 45.0 17 CTACGGC 105 0.0 45.0 6 TGTTCCG 20 7.021256E-4 45.0 1 >>END_MODULE