##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933372.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411386
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.379786866835527	31.0	28.0	31.0	25.0	34.0
2	28.46457584847321	31.0	26.0	33.0	16.0	34.0
3	28.443797309582727	31.0	26.0	33.0	16.0	34.0
4	33.05720661373989	35.0	32.0	37.0	28.0	37.0
5	34.215461877652615	35.0	33.0	37.0	30.0	37.0
6	33.45199156023783	35.0	32.0	37.0	28.0	37.0
7	33.55964714404477	35.0	32.0	37.0	29.0	37.0
8	33.39436441687369	35.0	33.0	37.0	28.0	37.0
9	34.671583379113535	37.0	34.0	39.0	28.0	39.0
10	34.47103693368272	37.0	32.0	39.0	27.0	39.0
11	34.627663556854145	37.0	33.0	39.0	27.0	39.0
12	34.64555429693767	37.0	33.0	39.0	27.0	39.0
13	34.66125001823105	37.0	33.0	39.0	27.0	39.0
14	35.54303987009767	38.0	34.0	40.0	27.0	41.0
15	35.775434749845644	38.0	34.0	40.0	27.0	41.0
16	35.79134924377592	38.0	34.0	40.0	27.0	41.0
17	35.08193764493687	37.0	33.0	40.0	27.0	41.0
18	34.97312499696149	37.0	33.0	39.0	27.0	40.0
19	34.31957334474192	37.0	32.0	38.0	27.0	40.0
20	33.61860393887979	35.0	32.0	38.0	25.0	40.0
21	34.20574351096051	35.0	33.0	38.0	27.0	40.0
22	34.83030292717788	35.0	33.0	39.0	29.0	40.0
23	35.125373736588024	35.0	34.0	39.0	30.0	40.0
24	34.72002936414948	35.0	33.0	39.0	28.0	40.0
25	33.7513746214018	35.0	33.0	38.0	24.0	40.0
26	33.79328659701594	35.0	32.0	38.0	25.0	40.0
27	34.41408069307171	35.0	33.0	39.0	27.0	40.0
28	34.11202860573768	35.0	33.0	39.0	25.0	40.0
29	34.078191771231886	35.0	33.0	39.0	25.0	40.0
30	33.48291871867298	35.0	33.0	39.0	23.0	40.0
31	33.49919297205058	35.0	33.0	39.0	23.0	40.0
32	32.93387232428911	35.0	32.0	39.0	20.0	40.0
33	32.388399216307796	35.0	31.0	39.0	15.0	40.0
34	31.904729378248167	35.0	30.0	39.0	12.0	40.0
35	31.569866256994647	35.0	30.0	39.0	10.0	41.0
36	31.137870515768647	35.0	29.0	39.0	8.0	40.0
37	31.02044065670684	35.0	28.0	39.0	8.0	40.0
38	30.73672414715134	35.0	27.0	39.0	8.0	40.0
39	30.332096862800388	35.0	25.0	39.0	7.0	40.0
40	30.0142834223819	35.0	23.0	39.0	7.0	40.0
41	29.44901139076196	35.0	22.0	39.0	7.0	40.0
42	29.849241345111405	35.0	23.0	39.0	7.0	40.0
43	29.795804913147265	35.0	23.0	39.0	7.0	40.0
44	29.99985658238248	35.0	23.0	39.0	7.0	40.0
45	30.081468499171095	35.0	23.0	39.0	7.0	40.0
46	29.875187779846666	35.0	23.0	39.0	7.0	40.0
47	29.70880632787698	35.0	23.0	39.0	7.0	40.0
48	29.631188227115167	35.0	23.0	38.0	7.0	40.0
49	29.880163641932395	35.0	24.0	39.0	7.0	40.0
50	29.738479189860616	35.0	24.0	39.0	7.0	40.0
51	28.489693377995362	33.0	22.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	12.0
10	18.0
11	25.0
12	28.0
13	39.0
14	60.0
15	122.0
16	196.0
17	378.0
18	814.0
19	1447.0
20	2267.0
21	3383.0
22	4778.0
23	6960.0
24	9945.0
25	14338.0
26	18463.0
27	19859.0
28	19708.0
29	19046.0
30	20200.0
31	22771.0
32	26023.0
33	30257.0
34	33447.0
35	37751.0
36	40872.0
37	41491.0
38	31953.0
39	4726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.35109118929667	2.4203546061363292	31.086619379366336	35.141934825200664
2	34.52645447341426	19.989985074844547	31.80297822483021	13.680582226910978
3	12.382045086609656	20.140695113591615	53.894882178781	13.58237762101773
4	11.450073653454421	2.8654353818554834	69.79576358942697	15.888727375263134
5	24.50934159159524	3.321940950834496	54.334129017516396	17.834588440053867
6	13.727253722781038	17.38051367815142	58.10382463185427	10.788407967213274
7	52.22613312071874	1.358091913677179	43.55957665063955	2.8561983149645345
8	50.826231325324635	13.5126134579203	31.789851866616754	3.871303350138313
9	47.743724871531846	2.9799263951617214	30.871492953090286	18.40485578021615
10	32.02053545818283	17.212301828453082	37.45241695147623	13.314745761887862
11	27.68859416703534	15.055446709416461	43.445571798748624	13.810387324799581
12	21.32328275634076	13.194663892305522	45.89509608980374	19.58695726154998
13	19.818370095239022	14.350512657212446	53.85161381281813	11.979503434730399
14	16.765519487780335	20.549313783162283	45.022193268609044	17.662973460448338
15	12.375968068918242	14.797781159300511	57.11934776584522	15.706903005936033
16	15.908659993290971	16.936891386678205	43.52603151298294	23.628417107047884
17	16.84330531423043	17.1165280296364	49.721186428317935	16.31898022781524
18	16.463370168163234	16.118924805413894	48.00236274447842	19.41534228194445
19	16.492296772374363	19.157919812536157	44.075393912286756	20.27438950280272
20	17.366658077814996	19.470764683290145	50.621071208062496	12.541506030832355
21	17.414544977223336	23.817776978312338	45.26260008848138	13.505077955982944
22	14.840077202432752	14.722669220634636	49.977393494187936	20.459860082744676
23	15.388953440321254	22.767668321236016	47.49140709698434	14.351971141458385
24	17.974359846956386	17.604391009903107	43.31041892529158	21.11083021784893
25	15.806809176782878	26.62049753759243	41.142139012995095	16.4305542726296
26	15.642000456991731	15.44729281015883	46.66007107679892	22.25063565605052
27	18.317589806167444	18.47389070119061	43.298507970616406	19.910011522025542
28	13.261024925495763	18.63553937178222	48.132167842367025	19.971267860354995
29	22.3240460297628	16.930085126863823	42.03667601717122	18.709192826202155
30	19.34727968380062	21.11933804261691	44.906243771056864	14.627138502525607
31	20.02741950382366	17.375408983290633	40.1209569601299	22.476214552755806
32	22.79659492544715	24.336025047036117	38.67754371806527	14.189836309451465
33	18.730583928475934	17.744891658928598	39.61073055475879	23.91379385783668
34	23.827986368033915	23.617721555910993	37.02192101821647	15.532371057838624
35	19.910011522025542	25.51739728624698	35.34466413538623	19.227927056341247
36	21.622028946050666	22.017278176700227	32.74126975638452	23.61942312086459
37	20.989289864020652	27.636817976304496	35.34053176335607	16.033360396318784
38	19.86722931747799	22.60431808569081	34.59257242589685	22.935880170934354
39	21.007277836387235	28.635636604065283	32.31028766170944	18.04679789783804
40	20.314254738858395	24.69651373649079	37.67070342695182	17.318528097698998
41	18.320020613244008	26.702172655365036	31.08370241087446	23.894104320516497
42	17.800800221689606	25.502569363079928	38.15808024580321	18.538550169427253
43	20.764683290145992	20.396659098753968	33.13603282561876	25.702624785481277
44	22.664845181897295	21.312830285911527	34.707792681326055	21.314531850865123
45	17.70089405084276	20.977865070760792	34.33028834233542	26.990952536061023
46	23.455100562488756	24.455134593787832	31.715469170073845	20.374295673649563
47	15.589251943430257	20.944563013811845	44.12085000461853	19.345335038139364
48	19.753224465587063	25.584730642267843	32.24368354781155	22.418361344333544
49	18.134793114009714	21.279771309670238	39.56964991516483	21.015785661155217
50	19.74155659161955	19.887161935505826	36.10258978185937	24.268691691015253
51	19.302309752884153	21.244524607060036	33.820061936964315	25.633103703091503
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	42.0
1	582.0
2	1122.0
3	13804.0
4	26486.0
5	18943.5
6	11401.0
7	10689.5
8	9978.0
9	9568.5
10	9159.0
11	8751.0
12	8343.0
13	7760.5
14	7178.0
15	6701.5
16	6225.0
17	5715.5
18	5206.0
19	4804.0
20	4402.0
21	4172.0
22	3942.0
23	3819.0
24	3696.0
25	3745.0
26	3869.0
27	3944.0
28	4385.5
29	4827.0
30	5137.5
31	5448.0
32	6176.5
33	6905.0
34	7300.5
35	7696.0
36	7978.5
37	8261.0
38	8961.0
39	9661.0
40	10549.0
41	11437.0
42	13189.0
43	14941.0
44	16329.0
45	17717.0
46	42374.0
47	67031.0
48	46679.5
49	26328.0
50	24329.0
51	22330.0
52	19747.0
53	17164.0
54	17363.5
55	17563.0
56	17002.0
57	16441.0
58	15180.0
59	13919.0
60	12560.5
61	11202.0
62	10045.0
63	8888.0
64	7586.5
65	6285.0
66	5207.0
67	4129.0
68	3434.0
69	2739.0
70	2384.0
71	2029.0
72	1726.0
73	1423.0
74	1145.5
75	709.0
76	550.0
77	440.0
78	330.0
79	244.5
80	159.0
81	125.5
82	92.0
83	80.5
84	69.0
85	46.5
86	24.0
87	16.5
88	9.0
89	5.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	411386.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.40594200703487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53126916586942	35.38941541173678
2	5.909792379247717	5.13040210574486
3	2.720639770716246	3.5427579632922606
4	1.9038195604327786	3.305483257280152
5	1.5019264174236782	3.2596265486762905
6	1.235142000243133	3.2167501219803887
7	1.025224178542421	3.11505748666156
8	0.8160603773929102	2.8337495532284516
9	0.6756330292255909	2.639383927614296
>10	2.574917622998546	16.378099172651176
>50	0.06210323406326906	1.8727411393561002
>100	0.033874491307237665	2.5167562812335973
>500	0.003952023985844394	1.1454059258506166
>1k	0.0033874491307237667	3.5222334985560364
>5k	0.0	0.0
>10k+	0.002258299420482511	12.132137606137459
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCG	13586	3.302494494221971	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13091	3.1821695439319764	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGC	11768	2.860573767702353	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTC	11062	2.6889587880968238	No Hit
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	2502	0.6081879305567034	Illumina PCR Primer Index 6 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCT	2484	0.6038124778188855	Illumina PCR Primer Index 6 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCATAACT	2459	0.5977354601274716	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTC	2419	0.5880122318212093	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCATAACTCGTA	2400	0.5833936983757347	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCT	2109	0.5126572124476768	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGCATAACTCGT	919	0.22339117033637507	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTT	778	0.18911679055680067	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATCTGTCTCTT	669	0.16262099342223604	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATCTGT	669	0.16262099342223604	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATCT	598	0.14536226317862058	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGCATAACTCGTAT	537	0.13053434001157063	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATG	504	0.1225126766589043	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09455839527840033	0.0
2	0.0	0.0	0.0	0.8935646813455004	0.0
3	0.0	0.0	0.0	1.011944985974243	0.0
4	0.0	0.0	0.0	1.8338008585610595	0.0
5	0.0	0.0	0.0	5.277039082516177	0.0
6	0.0	0.0	0.0	5.595231728838609	0.0
7	0.0	0.0	0.0	6.32641849746953	0.0
8	0.0	0.0	0.0	7.160914566854487	0.0
9	0.0	0.0	0.0	7.287316534835896	0.0
10	0.0	0.0	0.0	11.501120602062297	0.0
11	0.0	0.0	0.0	11.72815798301352	0.0
12	0.0	0.0	0.0	15.567374679741167	0.0
13	0.0	0.0	0.0	15.84254204080839	0.0
14	0.0	0.0	0.0	16.060342354868663	0.0
15	0.0	0.0	0.0	16.842819152815117	0.0
16	0.0	0.0	0.0	17.19747390528603	0.0
17	0.0	0.0	0.0	17.379541355320793	0.0
18	0.0	0.0	0.0	17.54216234874303	0.0
19	0.0	0.0	0.0	18.407286587292713	0.0
20	0.0	0.0	0.0	18.61220362384719	0.0
21	0.0	0.0	0.0	18.75902437127175	0.0
22	0.0	0.0	0.0	19.01717608280301	0.0
23	0.0	0.0	0.0	19.140661082292542	0.0
24	0.0	0.0	0.0	19.270223099473487	0.0
25	0.0	0.0	0.0	19.370858512443302	0.0
26	0.0	0.0	0.0	19.518165421283175	0.0
27	0.0	0.0	0.0	19.708497615378256	0.0
28	0.0	0.0	0.0	19.82493327434575	0.0
29	0.0	0.0	0.0	19.968350891863118	0.0
30	0.0	0.0	0.0	20.11565780070299	0.0
31	0.0	0.0	0.0	20.23549658957767	0.0
32	0.0	0.0	0.0	20.363843203220334	0.0
33	0.0	0.0	0.0	20.49462062393956	0.0
34	0.0	0.0	0.0	20.71631022932234	0.0
35	0.0	0.0	0.0	20.887682128220213	0.0
36	0.0	0.0	0.0	21.03741984413665	0.0
37	0.0	0.0	0.0	21.19420690057513	0.0
38	0.0	0.0	0.0	21.407874842605242	0.0
39	0.0	0.0	0.0	21.808714929530904	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	25	3.886423E-5	45.000004	14
TCATACC	25	3.886423E-5	45.000004	27
TAATACC	20	7.027999E-4	45.0	4
CGTATAC	20	7.027999E-4	45.0	32
CCGATTA	20	7.027999E-4	45.0	18
CGTTCAT	20	7.027999E-4	45.0	17
CTCGACA	20	7.027999E-4	45.0	30
CACAACG	20	7.027999E-4	45.0	12
TAAGCTA	20	7.027999E-4	45.0	13
CGTTTTT	9465	0.0	44.500793	1
GAATGAC	295	0.0	43.474575	1
CACCGGT	110	0.0	42.954544	16
CGATGAA	320	0.0	42.890625	19
GTATGAT	105	0.0	42.857143	1
GATACCT	1660	0.0	42.831326	5
TACGGCT	1595	0.0	42.31975	7
ACGGCTG	1595	0.0	42.31975	8
CGGCTGT	1595	0.0	42.31975	9
CCGATGA	325	0.0	42.230766	18
TGATACC	1695	0.0	41.9469	4
>>END_MODULE