FastQCFastQC Report
Sat 14 Jan 2017
SRR2933371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306275
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216827.079258836013387No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCG60421.972736919435148No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGC44281.4457595298342993No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTC28970.945881968818872No Hit
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC12860.4198840910946045Illumina PCR Primer Index 6 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCATAACT12120.3957227981389274No Hit
GAACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCT8990.29352705901559056Illumina PCR Primer Index 6 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCATAACTCGTA7660.2501020324871439No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTC7050.23018529099665333No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCT4560.14888580524038852No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGGCATAACTCGTATGCCG4280.1397436943922945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC302.1602682E-645.00000416
AGCCGTT207.0251734E-445.00000428
TAGCATA207.0251734E-445.00000430
ATCCCCG351.2080272E-745.00000438
GTCTTAT800.045.00000423
CGATGAA1350.045.019
GCTACGG750.045.05
TCGGGTA253.8840837E-544.9999965
TTCGGGT502.1827873E-1144.9999964
CGTTTTT83550.044.5421941
ACCACCG3050.043.5245914
TACGGCT8350.042.574857
ACGGCTG8350.042.574858
CCGATGA1450.041.8965518
ACCGGTG700.041.78571717
CGACCAC5400.041.66666412
GCGATCT650.041.538469
TTACACG1250.041.434
GCGACCA5600.041.3839311
GATACCT9250.041.108115
Produced by FastQC (version 0.11.5)