##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933371.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	306275
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.468167496530896	31.0	27.0	31.0	16.0	33.0
2	28.64494000489756	31.0	30.0	33.0	16.0	34.0
3	28.82970859521672	31.0	30.0	33.0	16.0	34.0
4	31.90527793649498	35.0	28.0	37.0	19.0	37.0
5	33.617161048077705	35.0	33.0	37.0	28.0	37.0
6	32.537977307974856	35.0	33.0	37.0	17.0	37.0
7	31.990645661578647	35.0	31.0	35.0	25.0	37.0
8	32.73692923026692	35.0	32.0	35.0	26.0	37.0
9	32.830182025957065	35.0	31.0	37.0	25.0	39.0
10	31.2783380948494	35.0	27.0	39.0	15.0	39.0
11	31.873597257366747	35.0	27.0	39.0	15.0	39.0
12	32.78703126275406	35.0	31.0	39.0	21.0	39.0
13	32.80250102032487	35.0	31.0	39.0	21.0	39.0
14	33.18389355970941	36.0	31.0	40.0	18.0	41.0
15	33.739642478165045	36.0	31.0	40.0	22.0	41.0
16	34.03185046118684	36.0	32.0	40.0	24.0	41.0
17	32.08280466900661	35.0	27.0	39.0	18.0	41.0
18	32.81478736429679	36.0	30.0	39.0	21.0	39.0
19	32.59044975920333	36.0	30.0	37.0	19.0	39.0
20	32.162651212145946	34.0	30.0	36.0	23.0	39.0
21	32.587367561831684	35.0	31.0	37.0	23.0	39.0
22	32.881436617418984	34.0	30.0	37.0	25.0	39.0
23	33.88458738062199	35.0	32.0	38.0	27.0	40.0
24	32.2139253938454	35.0	31.0	37.0	21.0	39.0
25	31.45114031507632	34.0	30.0	37.0	18.0	39.0
26	30.707700595869724	33.0	25.0	37.0	18.0	39.0
27	32.26719777977308	34.0	30.0	37.0	22.0	39.0
28	32.29949555138356	35.0	31.0	37.0	21.0	39.0
29	32.4560444045384	35.0	31.0	38.0	21.0	40.0
30	31.474883682964656	34.0	29.0	37.0	18.0	40.0
31	31.81832993225043	35.0	30.0	37.0	20.0	40.0
32	31.55910864419231	35.0	30.0	37.0	18.0	40.0
33	31.502306750469348	35.0	30.0	38.0	15.0	40.0
34	30.71925883601339	35.0	29.0	38.0	12.0	40.0
35	30.236333360541998	35.0	27.0	38.0	10.0	40.0
36	29.715507305526078	34.0	25.0	38.0	8.0	40.0
37	29.75274508203412	35.0	25.0	38.0	8.0	40.0
38	29.67435801159089	34.0	24.0	38.0	8.0	40.0
39	29.039376377438575	34.0	23.0	37.0	7.0	40.0
40	28.822904252714064	33.0	23.0	37.0	7.0	40.0
41	28.289164966125213	33.0	21.0	37.0	7.0	40.0
42	28.80270998285854	33.0	23.0	37.0	7.0	40.0
43	28.851323157293283	34.0	22.0	38.0	7.0	40.0
44	28.897386335809323	34.0	22.0	38.0	7.0	40.0
45	29.175923598073627	34.0	23.0	38.0	7.0	40.0
46	28.88451554975104	34.0	23.0	38.0	7.0	40.0
47	28.897889151905968	34.0	23.0	38.0	7.0	40.0
48	28.704158027916087	34.0	23.0	37.0	7.0	40.0
49	28.63194514733491	34.0	23.0	37.0	7.0	39.0
50	28.750675046934944	34.0	23.0	37.0	7.0	40.0
51	27.12667374091911	31.0	20.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	16.0
11	21.0
12	22.0
13	40.0
14	78.0
15	199.0
16	398.0
17	698.0
18	1158.0
19	1810.0
20	2679.0
21	3841.0
22	5182.0
23	7016.0
24	9468.0
25	12961.0
26	16230.0
27	17594.0
28	17923.0
29	18581.0
30	20292.0
31	22341.0
32	24784.0
33	26698.0
34	29263.0
35	31101.0
36	21851.0
37	11482.0
38	2486.0
39	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.476369275977472	2.4536772508366664	33.84964492694474	39.22030854624112
2	39.64117214921231	12.640600767284305	33.99395967676108	13.724267406742307
3	13.098032813647867	12.664109052322258	60.39441678230348	13.84344135172639
4	12.157701412129622	2.982287160231818	68.47343074034772	16.386580687290834
5	19.933393192392458	3.3571137050036732	59.72181862705085	16.987674475553018
6	14.103991510897071	11.658150355073055	62.37858134029876	11.859276793731125
7	52.58019753489511	1.4454330258754386	43.09558403395641	2.878785405273039
8	53.55677087584687	8.967431230103665	33.78565015100808	3.6901477430413845
9	49.49212309199249	2.9339645743204636	34.0879928169129	13.485919516774143
10	29.578320137131662	18.041302750795854	39.89323320545262	12.487143906619867
11	24.86686801077463	15.962452044731043	46.496449269447396	12.674230675046935
12	20.393437270426904	13.903844584115582	48.71798220553424	16.984735939923272
13	18.744265774222512	14.849073545016733	54.17908742143498	12.22757325932577
14	15.74630642396539	17.3820912578565	49.43596441106848	17.435637907109623
15	12.638315239572279	15.109623704187413	56.6481103583381	15.60395069790221
16	15.895518733164641	14.810546077871194	47.791363970288145	21.502571218676025
17	16.52175332625908	15.7015753816015	51.70843196473758	16.068239327401844
18	16.270671781895356	14.476206023998042	50.74785731776997	18.505264876336625
19	16.75226512121459	17.490164068239327	47.32446330911762	18.433107501428456
20	17.22438984572688	18.075585666476208	51.98791935352216	12.712105134274754
21	17.829075177536527	20.262182678964983	47.65455881152559	14.2541833319729
22	15.265366092563873	14.853644600440779	51.642804669006615	18.238184637988734
23	15.213125459146193	20.420210595053465	49.3011182760591	15.065545669741246
24	16.408129948575624	16.752591625173455	47.53603787445923	19.30324055179169
25	15.841972083911518	21.30960737898947	45.4565341604767	17.391886376622317
26	15.314015182434087	16.186107256550486	49.13133621745164	19.36854134356379
27	15.924251081544364	17.46502326340707	47.98106277038609	18.629662884662476
28	13.403314015182435	17.840502816096645	50.48273610317525	18.27344706554567
29	19.103420128969066	16.67063913149947	46.476532527956905	17.749408211574565
30	17.288058117704676	19.238919271896172	48.354583299322506	15.118439311076648
31	18.395886050118357	17.514651865153866	44.817565912986694	19.271896171741083
32	20.178924169455556	21.601175414251898	43.32870786058281	14.891192555709736
33	17.853236470492202	17.872826708023833	43.02277365113052	21.25116317035344
34	21.216880254673086	21.64133540119174	41.09966533344217	16.042119010693003
35	18.530732185127743	23.578483389111092	39.30813811117459	18.582646314586565
36	20.288302995673824	21.293282181046443	37.012815280385276	21.405599542894457
37	19.590890539547793	25.783691127254922	38.14153946616602	16.48387886703126
38	19.124969390253856	22.494163741735367	38.540527303893555	19.840339564117215
39	19.154354746551302	26.21010529752673	36.7166761896988	17.918863766223165
40	19.593502571218675	23.765243653579297	39.473349114358015	17.167904660844012
41	17.96000326503959	24.20243245449351	35.6875357113705	22.1500285690964
42	17.539792669986124	23.98073626642723	39.47302261039915	19.006448453187495
43	19.486082768753572	20.2066770059587	37.24202105950535	23.065219165782384
44	21.324300057138192	21.189453922128806	37.69977960982777	19.78646641090523
45	17.487225532609582	21.03599706146437	37.48722553260958	23.989551873316465
46	20.496612521426822	23.66794547383887	35.85568525018366	19.97975675455065
47	16.074769406579055	20.975920333034036	44.05387315321198	18.895437107174924
48	18.52648763366256	24.27165129377194	36.624928577259	20.576932495306504
49	17.703044649416373	21.43302587543874	40.51652926291731	20.347400212227573
50	18.604848583789078	20.272957309607378	38.30381193371969	22.818382172883847
51	18.25483633989062	21.276303975185698	37.386335809321686	23.082523875601993
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62.0
1	332.0
2	602.0
3	15725.0
4	30848.0
5	20070.5
6	9293.0
7	8659.0
8	8025.0
9	7727.5
10	7430.0
11	7043.5
12	6657.0
13	6130.5
14	5604.0
15	5210.5
16	4817.0
17	4402.5
18	3988.0
19	3669.0
20	3350.0
21	3086.5
22	2823.0
23	2702.5
24	2582.0
25	2630.5
26	2777.0
27	2875.0
28	3148.5
29	3422.0
30	3639.5
31	3857.0
32	4553.0
33	5249.0
34	5389.5
35	5530.0
36	5925.0
37	6320.0
38	6896.0
39	7472.0
40	8021.0
41	8570.0
42	9622.0
43	10674.0
44	11850.0
45	13026.0
46	21856.5
47	30687.0
48	26025.5
49	21364.0
50	19939.5
51	18515.0
52	16453.0
53	14391.0
54	13733.5
55	13076.0
56	12403.5
57	11731.0
58	10885.5
59	10040.0
60	9159.0
61	8278.0
62	7341.0
63	6404.0
64	5712.0
65	5020.0
66	4252.5
67	3485.0
68	2930.5
69	2376.0
70	2090.0
71	1804.0
72	1570.0
73	1336.0
74	1072.5
75	686.0
76	563.0
77	435.0
78	307.0
79	237.5
80	168.0
81	128.0
82	88.0
83	74.5
84	61.0
85	35.0
86	9.0
87	8.0
88	7.0
89	3.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	306275.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.693848883726666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.52785546816925	37.60155514161278
2	6.831437322969363	6.379722040347634
3	3.1578475485716564	4.423561686925549
4	2.0733775018624145	3.8725590300352914
5	1.6062270970921082	3.7500462672282935
6	1.2505957824484304	3.503707828816375
7	0.9588406724466715	3.134037302395702
8	0.7791562467953841	2.9105443235740247
9	0.6209701581789644	2.609593805458117
>10	2.101965734452402	14.116072861091048
>50	0.05362470379244197	1.7204729741572686
>100	0.031751469350788014	2.826820780978431
>500	0.0021167646233858674	0.7808351108297064
>1k	0.002822352831181156	3.2363473812996655
>5k	7.05588207795289E-4	1.9906353331785174
>10k+	7.05588207795289E-4	7.143488132071601
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21682	7.079258836013387	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCG	6042	1.972736919435148	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGC	4428	1.4457595298342993	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTC	2897	0.945881968818872	No Hit
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	1286	0.4198840910946045	Illumina PCR Primer Index 6 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCATAACT	1212	0.3957227981389274	No Hit
GAACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCT	899	0.29352705901559056	Illumina PCR Primer Index 6 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCATAACTCGTA	766	0.2501020324871439	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTC	705	0.23018529099665333	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCT	456	0.14888580524038852	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGGCATAACTCGTATGCCG	428	0.1397436943922945	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2650395886050116E-4	0.0	0.0	0.11264386580687291	0.0
2	3.2650395886050116E-4	0.0	0.0	0.7976491714962044	0.0
3	3.2650395886050116E-4	0.0	0.0	0.959595135091013	0.0
4	3.2650395886050116E-4	0.0	0.0	1.523140968084238	0.0
5	3.2650395886050116E-4	0.0	0.0	2.9728185454248632	0.0
6	3.2650395886050116E-4	0.0	0.0	3.2513264223328706	0.0
7	3.2650395886050116E-4	0.0	0.0	3.6160313443800507	0.0
8	3.2650395886050116E-4	0.0	0.0	4.120153456860664	0.0
9	3.2650395886050116E-4	0.0	0.0	4.285364460044078	0.0
10	3.2650395886050116E-4	0.0	0.0	7.08807444290262	0.0
11	3.2650395886050116E-4	0.0	0.0	7.5366908823769485	0.0
12	3.2650395886050116E-4	0.0	0.0	9.898947024732674	0.0
13	3.2650395886050116E-4	0.0	0.0	10.122928740510979	0.0
14	3.2650395886050116E-4	0.0	0.0	10.270508529915926	0.0
15	3.2650395886050116E-4	0.0	0.0	10.722063505019998	0.0
16	3.2650395886050116E-4	0.0	0.0	10.988490735450167	0.0
17	3.2650395886050116E-4	0.0	0.0	11.219655538323401	0.0
18	3.2650395886050116E-4	0.0	0.0	11.43286262345931	0.0
19	3.2650395886050116E-4	0.0	0.0	12.13615215084483	0.0
20	3.2650395886050116E-4	0.0	0.0	12.39735531793323	0.0
21	3.2650395886050116E-4	0.0	0.0	12.636029711860257	0.0
22	3.2650395886050116E-4	0.0	0.0	13.026854950616276	0.0
23	3.2650395886050116E-4	0.0	0.0	13.274344951432536	0.0
24	3.2650395886050116E-4	0.0	0.0	13.454575136723532	0.0
25	3.2650395886050116E-4	0.0	0.0	13.626316219084156	0.0
26	3.2650395886050116E-4	0.0	0.0	13.785650151008081	0.0
27	3.2650395886050116E-4	0.0	0.0	13.9805730144478	0.0
28	3.2650395886050116E-4	0.0	0.0	14.144151497836912	0.0
29	3.2650395886050116E-4	0.0	0.0	14.337768345441189	0.0
30	3.2650395886050116E-4	0.0	0.0	14.564362092890375	0.0
31	3.2650395886050116E-4	0.0	0.0	14.742959758387071	0.0
32	3.2650395886050116E-4	0.0	0.0	14.913721328871112	0.0
33	3.2650395886050116E-4	0.0	0.0	15.08807444290262	0.0
34	3.2650395886050116E-4	0.0	0.0	15.301281528038528	0.0
35	3.2650395886050116E-4	0.0	0.0	15.539629418006694	0.0
36	3.2650395886050116E-4	0.0	0.0	15.746632927924251	0.0
37	3.2650395886050116E-4	0.0	0.0	15.934699208227899	0.0
38	3.2650395886050116E-4	0.0	0.0	16.16978205860746	0.0
39	3.2650395886050116E-4	0.0	0.0	16.659537996898212	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGATC	30	2.1602682E-6	45.000004	16
AGCCGTT	20	7.0251734E-4	45.000004	28
TAGCATA	20	7.0251734E-4	45.000004	30
ATCCCCG	35	1.2080272E-7	45.000004	38
GTCTTAT	80	0.0	45.000004	23
CGATGAA	135	0.0	45.0	19
GCTACGG	75	0.0	45.0	5
TCGGGTA	25	3.8840837E-5	44.999996	5
TTCGGGT	50	2.1827873E-11	44.999996	4
CGTTTTT	8355	0.0	44.542194	1
ACCACCG	305	0.0	43.52459	14
TACGGCT	835	0.0	42.57485	7
ACGGCTG	835	0.0	42.57485	8
CCGATGA	145	0.0	41.89655	18
ACCGGTG	70	0.0	41.785717	17
CGACCAC	540	0.0	41.666664	12
GCGATCT	65	0.0	41.53846	9
TTACACG	125	0.0	41.4	34
GCGACCA	560	0.0	41.38393	11
GATACCT	925	0.0	41.10811	5
>>END_MODULE