FastQCFastQC Report
Sat 14 Jan 2017
SRR2933369.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933369.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183470
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113886.207009320324849No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCG34451.8776911756690469No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC27001.471630239276176No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTC15850.8639014552787921No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGATCGT9350.509620101378972No Hit
GCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC7890.43004305881070476No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC6310.34392543740121No Hit
GAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT5620.30631710906415216No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTA5300.28887556548754567No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTC4700.2561726712814084No Hit
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT3670.20003270289420616No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCT3510.1913119311059029No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTAGGATCGTCGTATGCCG2860.15588379571592087No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC2750.14988826511146236No Hit
CCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC2700.1471630239276176No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGT2130.11609527443178722No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2060.11227993677440454No Hit
GAATCTTTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTC2030.11064479206409766No Hit
TCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC1900.10355916498610127No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTC253.8779548E-545.00000417
GGTATGC253.8779548E-545.0000048
CTCGCGG253.8779548E-545.0000042
CTCTGCG253.8779548E-545.0000041
TCAAGCG253.8779548E-545.00000417
GCGAGAC207.017777E-445.021
GACACGA207.017777E-445.025
CATTGGG207.017777E-445.03
CGTGAGG207.017777E-445.045
TAAACGA207.017777E-445.031
CGACCAA207.017777E-445.029
CGATTGG207.017777E-445.010
AAACGAC207.017777E-445.032
GCCGATT207.017777E-445.09
CGTAGGG207.017777E-445.03
CTACGGC207.017777E-445.06
ACGTGAG207.017777E-445.044
AGACACG207.017777E-445.024
CGTTTTT51050.044.294811
TACGGCT3950.043.291147
Produced by FastQC (version 0.11.5)