##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933369.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	183470
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.471183299722025	31.0	28.0	31.0	16.0	33.0
2	28.71693464871641	31.0	30.0	33.0	16.0	34.0
3	28.880923311713087	31.0	30.0	33.0	16.0	34.0
4	31.904758271106992	35.0	30.0	37.0	19.0	37.0
5	33.59089769444596	35.0	33.0	37.0	28.0	37.0
6	32.565640159154086	35.0	33.0	37.0	17.0	37.0
7	31.985436311113535	35.0	31.0	35.0	25.0	37.0
8	32.72974328228048	35.0	32.0	35.0	26.0	37.0
9	32.797863410911866	35.0	31.0	37.0	25.0	39.0
10	31.853392925273887	35.0	28.0	39.0	15.0	39.0
11	32.50705837466616	35.0	30.0	39.0	17.0	39.0
12	33.33937428462419	35.0	31.0	39.0	25.0	39.0
13	33.45248269471848	35.0	32.0	39.0	25.0	39.0
14	33.47926636507331	36.0	31.0	40.0	19.0	41.0
15	34.12772660380444	36.0	32.0	40.0	25.0	41.0
16	34.26910121545757	37.0	32.0	40.0	25.0	41.0
17	32.10382623862212	35.0	27.0	39.0	18.0	41.0
18	32.923720499264185	36.0	30.0	39.0	22.0	39.0
19	32.76055485910503	36.0	30.0	37.0	20.0	39.0
20	32.450738540360824	34.0	30.0	37.0	24.0	39.0
21	32.83881288494032	35.0	31.0	37.0	24.0	39.0
22	33.10134081866245	35.0	31.0	38.0	25.0	39.0
23	34.080171145146345	35.0	33.0	38.0	27.0	40.0
24	32.39896440835014	35.0	31.0	37.0	21.0	39.0
25	31.427617594157084	35.0	29.0	37.0	18.0	39.0
26	30.79778165367635	33.0	25.0	37.0	19.0	39.0
27	32.487349430424594	35.0	30.0	38.0	22.0	39.0
28	32.48409004196871	35.0	31.0	38.0	21.0	39.0
29	32.76035864173979	35.0	31.0	38.0	22.0	40.0
30	31.561563198343052	35.0	29.0	38.0	18.0	40.0
31	32.00257262767755	35.0	30.0	37.0	21.0	40.0
32	31.94482476699188	35.0	30.0	38.0	19.0	40.0
33	31.911544121654767	35.0	30.0	38.0	16.0	40.0
34	31.11580094838393	35.0	30.0	38.0	12.0	40.0
35	30.59233117130866	35.0	28.0	38.0	10.0	40.0
36	30.045413419087588	35.0	25.0	38.0	8.0	40.0
37	30.142497411020877	35.0	26.0	38.0	8.0	40.0
38	30.07661743064261	35.0	25.0	38.0	8.0	40.0
39	29.66416852891481	35.0	24.0	38.0	8.0	40.0
40	29.409478388837414	34.0	23.0	38.0	8.0	40.0
41	28.88341418215512	34.0	22.0	38.0	7.0	40.0
42	29.259840845914862	34.0	23.0	38.0	7.0	40.0
43	29.20318308170273	34.0	23.0	38.0	7.0	40.0
44	29.11150596827819	34.0	22.0	38.0	7.0	40.0
45	29.32835885975909	35.0	23.0	38.0	7.0	40.0
46	28.984978470594648	34.0	22.0	38.0	7.0	40.0
47	28.977767482422195	34.0	22.0	38.0	7.0	40.0
48	28.775096746062026	34.0	22.0	38.0	7.0	40.0
49	28.592843516651225	34.0	22.0	37.0	7.0	40.0
50	28.677396849621193	34.0	22.0	37.0	7.0	40.0
51	27.006655038970948	32.0	20.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	15.0
11	11.0
12	14.0
13	44.0
14	42.0
15	114.0
16	219.0
17	373.0
18	621.0
19	1022.0
20	1591.0
21	2180.0
22	2872.0
23	4076.0
24	5444.0
25	7445.0
26	9220.0
27	9939.0
28	9945.0
29	10295.0
30	11170.0
31	12893.0
32	14818.0
33	16807.0
34	18696.0
35	20299.0
36	14365.0
37	7407.0
38	1493.0
39	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.508475500081758	2.5448302174742468	34.90815937210443	39.038534910339564
2	40.19294707581621	12.098980759797243	34.259006922112604	13.449065242273942
3	13.055540415326755	12.004142366599444	60.812666921022505	14.12765029705129
4	11.995421594811141	3.0631710906415215	68.19425519158445	16.747152122962884
5	19.164441053033194	3.4474300975636343	60.2632582983594	17.124870551043767
6	14.021910939118113	11.428026380334659	62.65656510601188	11.893497574535346
7	52.25758979669701	1.4427426827274215	43.081157682454894	3.2185098381206734
8	52.904562053741756	8.611762140949473	34.255191584455225	4.228484220853545
9	48.424810595737725	3.403826238622118	34.7893388564888	13.38202430915136
10	26.095274431787214	20.673679620646425	40.23927617594157	12.991769771624789
11	21.765411238894643	17.902654384913063	46.87414836213005	13.457786014062245
12	17.173379844116205	15.828745844007194	49.871368616122524	17.126505695754073
13	17.04856379789611	16.47353790810487	53.67308006758598	12.804818226413037
14	14.365291328282554	19.752003052270126	48.246579822314274	17.636125797133044
15	11.803564615468469	17.34834032811904	54.64163078432441	16.20646427208808
16	13.453425628168091	16.520957104703765	48.31689104485747	21.708726222270673
17	13.804436692647299	17.657382678367036	50.886248432986314	17.651932195999347
18	13.759742737232244	16.533493214149452	50.518340873167276	19.188423175451028
19	14.568049272360604	18.74093857306372	47.74350029977653	18.94751185479915
20	16.55638524009375	18.244944677603968	51.73870387529297	13.45996620700932
21	17.225159426609256	21.07810541232899	46.86815283152559	14.828582329536163
22	14.511909303973402	17.491688014389272	49.27890118275467	18.71750149888265
23	14.83621300485093	21.930015806398867	47.24151087371232	15.992260315037882
24	15.259170436583638	18.40900419687142	46.99078868479861	19.341036681746335
25	13.701967624134737	22.24232844606748	45.76606529677876	18.289638633019024
26	13.922712160026162	19.049435875074945	49.53234861285224	17.495503352046658
27	14.765356734070965	20.522156210824658	46.851256336185756	17.861230718918623
28	13.442524663432714	20.16841990516161	50.00436038589415	16.384695045511528
29	16.60652967787649	18.01602441816101	48.62266310568486	16.75478279827765
30	14.956668665176867	22.726876328555075	47.29601569738922	15.020439308878835
31	17.903199433149833	19.385730637161387	44.82531204011555	17.885757889573227
32	18.290183681255794	22.893661089006375	43.35804218673353	15.458113043004307
33	16.58145745898512	21.57791464544612	41.83572246143783	20.004905434130922
34	17.786014062244508	21.697825257535293	43.637106883959234	16.87905379626097
35	15.641794298795444	25.323486128522376	39.32305008993296	19.711669482749222
36	20.260533057175557	23.228320706382515	39.4206137243146	17.090532512127325
37	19.728020929852292	25.38671172398757	38.6253883468687	16.259878999291438
38	20.758162097345615	26.057121055213383	35.66632146945005	17.518395377990952
39	20.654602932359513	23.8202430915136	39.31541941461819	16.209734561508693
40	17.058919714394722	25.42868043821878	38.14847113969586	19.36392870769063
41	19.794516814738106	24.055158881561017	38.28200795770426	17.86831634599662
42	18.250395159971657	23.349866463181993	38.058538180628986	20.341200196217365
43	20.887338529459857	23.041369161170763	36.94663977762032	19.12465253174906
44	19.329590668774184	23.975036790755983	37.53910721098817	19.15626532948166
45	18.60903689976563	22.285387256772225	36.946094729383546	22.1594811140786
46	21.441652586253884	24.219218400828474	36.380334659617375	17.958794353300267
47	16.785305499536708	23.734670518340874	41.493432168746935	17.986591813375483
48	19.144274268272742	23.24140186406497	37.37722788466779	20.237095982994497
49	17.977325993350412	21.178394287894477	39.924783343325885	20.919496375429226
50	19.67678639559601	20.84482476699188	38.08470049599389	21.393688341418216
51	18.563252847877038	20.8497302011228	37.48351229083774	23.103504660162425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	51.0
1	200.0
2	349.0
3	8585.0
4	16821.0
5	11278.5
6	5736.0
7	5454.0
8	5172.0
9	5150.0
10	5128.0
11	4826.5
12	4525.0
13	4180.0
14	3835.0
15	3634.5
16	3434.0
17	3200.0
18	2966.0
19	2776.5
20	2587.0
21	2440.5
22	2294.0
23	2258.5
24	2223.0
25	2131.0
26	2060.0
27	2081.0
28	2105.5
29	2130.0
30	2354.0
31	2578.0
32	2679.5
33	2781.0
34	3044.0
35	3307.0
36	3640.5
37	3974.0
38	4201.0
39	4428.0
40	4918.5
41	5409.0
42	6208.0
43	7007.0
44	7345.0
45	7683.0
46	13492.5
47	19302.0
48	16417.5
49	13533.0
50	12442.0
51	11351.0
52	9662.5
53	7974.0
54	7234.0
55	6494.0
56	6135.5
57	5777.0
58	5466.0
59	5155.0
60	4670.5
61	4186.0
62	3744.5
63	3303.0
64	2917.5
65	2532.0
66	2182.5
67	1833.0
68	1492.0
69	1151.0
70	1020.5
71	890.0
72	726.5
73	563.0
74	484.0
75	314.5
76	224.0
77	176.0
78	128.0
79	97.0
80	66.0
81	55.0
82	44.0
83	27.0
84	10.0
85	7.0
86	4.0
87	4.0
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	183470.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.57621568520439
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.575	43.97478578835344
2	8.38	9.146973748840255
3	4.085	6.688315232221798
4	2.513	5.4860012006767445
5	1.5150000000000001	4.134148338154232
6	0.958	3.137040877585548
7	0.5930000000000001	2.2654587130928343
8	0.363	1.5848933034983355
9	0.234	1.1493751023304044
>10	0.714	6.171478469682913
>50	0.033	1.2001309829176445
>100	0.027999999999999997	2.746275173279485
>500	0.005	1.8812421546689952
>1k	0.003	4.218741472466299
>5k	0.0	0.0
>10k+	0.001	6.2151394422310755
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11388	6.207009320324849	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCG	3445	1.8776911756690469	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC	2700	1.471630239276176	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTC	1585	0.8639014552787921	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGATCGT	935	0.509620101378972	No Hit
GCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	789	0.43004305881070476	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC	631	0.34392543740121	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	562	0.30631710906415216	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTA	530	0.28887556548754567	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTC	470	0.2561726712814084	No Hit
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT	367	0.20003270289420616	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCT	351	0.1913119311059029	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTAGGATCGTCGTATGCCG	286	0.15588379571592087	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC	275	0.14988826511146236	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	270	0.1471630239276176	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGT	213	0.11609527443178722	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	206	0.11227993677440454	No Hit
GAATCTTTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTC	203	0.11064479206409766	No Hit
TCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	190	0.10355916498610127	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2507221889137189	0.0
2	0.0	0.0	0.0	1.1129884994822041	0.0
3	0.0	0.0	0.0	1.3795170872622227	0.0
4	0.0	0.0	0.0	2.0962555186133973	0.0
5	0.0	0.0	0.0	3.5389982013408186	0.0
6	0.0	0.0	0.0	3.8742028669537256	0.0
7	0.0	0.0	0.0	4.333133482313185	0.0
8	0.0	0.0	0.0	4.916880143892734	0.0
9	0.0	0.0	0.0	5.104921785578024	0.0
10	0.0	0.0	0.0	7.678094511364256	0.0
11	0.0	0.0	0.0	8.622118057448084	0.0
12	0.0	0.0	0.0	11.003978852128414	0.0
13	0.0	0.0	0.0	11.338093421267782	0.0
14	0.0	0.0	0.0	11.536490979451681	0.0
15	0.0	0.0	0.0	12.109336676295852	0.0
16	0.0	0.0	0.0	12.478334332588433	0.0
17	0.0	0.0	0.0	12.89747642666376	0.0
18	0.0	0.0	0.0	13.231590995803128	0.0
19	0.0	0.0	0.0	14.123289911157137	0.0
20	0.0	0.0	0.0	14.455769335586199	0.0
21	0.0	0.0	0.0	14.771352264675423	0.0
22	0.0	0.0	0.0	15.240638796533494	0.0
23	0.0	0.0	0.0	15.513707963154738	0.0
24	0.0	0.0	0.0	15.758434621464	0.0
25	0.0	0.0	0.0	15.980269253828963	0.0
26	0.0	0.0	0.0	16.166675750803947	0.0
27	0.0	0.0	0.0	16.36343816427754	0.0
28	0.0	0.0	0.0	16.55475009538344	0.0
29	0.0	0.0	0.0	16.741701640595192	0.0
30	0.0	0.0	0.0	16.937919005832015	0.0
31	0.0	0.0	0.0	17.115059682781926	0.0
32	0.0	0.0	0.0	17.29601569738922	0.0
33	0.0	0.0	0.0	17.4666157954979	0.0
34	0.0	0.0	0.0	17.65520248541996	0.0
35	0.0	0.0	0.0	17.83288821060664	0.0
36	0.0	0.0	0.0	18.016569466397776	0.0
37	0.0	0.0	0.0	18.177358696244617	0.0
38	0.0	0.0	0.0	18.346868697879763	0.0
39	0.0	0.0	0.0	18.53164005014444	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCGTC	25	3.8779548E-5	45.000004	17
GGTATGC	25	3.8779548E-5	45.000004	8
CTCGCGG	25	3.8779548E-5	45.000004	2
CTCTGCG	25	3.8779548E-5	45.000004	1
TCAAGCG	25	3.8779548E-5	45.000004	17
GCGAGAC	20	7.017777E-4	45.0	21
GACACGA	20	7.017777E-4	45.0	25
CATTGGG	20	7.017777E-4	45.0	3
CGTGAGG	20	7.017777E-4	45.0	45
TAAACGA	20	7.017777E-4	45.0	31
CGACCAA	20	7.017777E-4	45.0	29
CGATTGG	20	7.017777E-4	45.0	10
AAACGAC	20	7.017777E-4	45.0	32
GCCGATT	20	7.017777E-4	45.0	9
CGTAGGG	20	7.017777E-4	45.0	3
CTACGGC	20	7.017777E-4	45.0	6
ACGTGAG	20	7.017777E-4	45.0	44
AGACACG	20	7.017777E-4	45.0	24
CGTTTTT	5105	0.0	44.29481	1
TACGGCT	395	0.0	43.29114	7
>>END_MODULE