##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933368.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	532810
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.207993468591056	31.0	28.0	31.0	25.0	34.0
2	28.17974512490381	31.0	25.0	33.0	16.0	34.0
3	28.17995345432706	31.0	25.0	33.0	16.0	34.0
4	32.89888890974269	35.0	32.0	37.0	28.0	37.0
5	34.13851654435915	35.0	33.0	37.0	30.0	37.0
6	33.3115406993112	35.0	32.0	37.0	28.0	37.0
7	33.49761453426174	35.0	32.0	37.0	29.0	37.0
8	33.25739194084195	35.0	32.0	37.0	28.0	37.0
9	34.56840149396596	37.0	34.0	39.0	28.0	39.0
10	34.44527880482724	37.0	32.0	39.0	27.0	39.0
11	34.626981475572904	37.0	33.0	39.0	27.0	39.0
12	34.733856346540044	37.0	33.0	39.0	27.0	39.0
13	34.784711247912014	37.0	33.0	39.0	27.0	39.0
14	35.568684897055235	38.0	34.0	40.0	27.0	41.0
15	35.76932302321653	38.0	34.0	40.0	27.0	41.0
16	35.83611981757099	38.0	34.0	40.0	27.0	41.0
17	35.066183067134624	37.0	33.0	40.0	27.0	41.0
18	34.90340834443798	37.0	33.0	39.0	27.0	40.0
19	34.232433700568684	37.0	32.0	38.0	27.0	40.0
20	33.45963288977309	35.0	32.0	37.0	25.0	40.0
21	34.04527692798558	35.0	33.0	38.0	27.0	40.0
22	34.65446219102494	35.0	33.0	39.0	28.0	40.0
23	34.905882021733824	35.0	33.0	39.0	29.0	40.0
24	34.51051969745313	35.0	33.0	39.0	27.0	40.0
25	33.528227698429085	35.0	32.0	38.0	24.0	40.0
26	33.59941817908823	35.0	32.0	38.0	24.0	40.0
27	34.25351438599125	35.0	33.0	38.0	27.0	40.0
28	33.91178093504251	35.0	33.0	39.0	24.0	40.0
29	33.858110771194234	35.0	33.0	39.0	24.0	40.0
30	33.28140800660648	35.0	32.0	39.0	22.0	40.0
31	33.381734577053734	35.0	33.0	39.0	22.0	40.0
32	32.78535500459826	35.0	32.0	39.0	18.0	40.0
33	32.106593344719506	35.0	31.0	39.0	15.0	40.0
34	31.809220923030725	35.0	31.0	39.0	12.0	40.0
35	31.374416771456993	35.0	30.0	39.0	10.0	40.0
36	31.01447607965316	35.0	28.0	39.0	8.0	40.0
37	30.907347835063156	35.0	27.0	39.0	8.0	40.0
38	30.494367598205738	35.0	25.0	39.0	8.0	40.0
39	30.225575721176405	35.0	24.0	39.0	7.0	40.0
40	29.848015239954204	35.0	23.0	39.0	7.0	40.0
41	29.359002270978397	35.0	22.0	38.0	7.0	40.0
42	29.72614815787992	35.0	23.0	39.0	7.0	40.0
43	29.5674217826242	35.0	22.0	39.0	7.0	40.0
44	29.74606144779565	35.0	23.0	39.0	7.0	40.0
45	29.825190968637976	35.0	23.0	39.0	7.0	40.0
46	29.527968694281263	35.0	23.0	39.0	7.0	40.0
47	29.376368686773898	35.0	23.0	38.0	7.0	40.0
48	29.33563934610837	35.0	23.0	38.0	7.0	40.0
49	29.528207053170924	35.0	23.0	38.0	7.0	40.0
50	29.4590060246617	35.0	23.0	39.0	7.0	40.0
51	28.262857303729284	33.0	20.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	29.0
10	28.0
11	39.0
12	63.0
13	63.0
14	101.0
15	143.0
16	233.0
17	533.0
18	1059.0
19	1990.0
20	3184.0
21	4758.0
22	6782.0
23	9168.0
24	13468.0
25	19172.0
26	24398.0
27	26563.0
28	25386.0
29	24903.0
30	26285.0
31	30005.0
32	34446.0
33	40095.0
34	44439.0
35	51350.0
36	52993.0
37	49799.0
38	35959.0
39	5360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.573731724254422	2.3548732193464836	29.87387624106154	36.197518815337546
2	35.346183442502955	20.85978116026351	30.431298211369906	13.36273718586363
3	12.08873707325313	21.198175709915354	53.88806516394212	12.825022052889398
4	10.703252566580957	2.7578311217882545	70.99941817908822	15.539498132542557
5	25.04344888421764	3.3569189767459315	54.85614008746082	16.743492051575608
6	13.042735684390308	18.169516337906572	57.761678647172545	11.026069330530582
7	50.195754584185735	1.366340721833299	45.73337587507742	2.7045288189035492
8	48.61076181002609	14.276759069837277	33.17111165330981	3.9413674668268235
9	45.912614252735494	2.728552392034684	32.21692535800755	19.141907997222273
10	31.41551397308609	16.768829413862353	38.10063624931965	13.715020363731911
11	27.14513616486177	14.47195060152775	44.23959760515005	14.143315628460426
12	19.447457817983896	13.723841519491002	46.884067491225764	19.944633171299337
13	18.441095324787447	15.051143934986206	54.83117809350425	11.676582646722096
14	15.497269195397983	21.781498095005723	45.8418573224977	16.87937538709859
15	11.45361385859875	14.17728646234117	57.42853925414313	16.94056042491695
16	14.565980368236332	17.080948180402018	44.39049567387999	23.962575777481653
17	15.210863159475235	18.189786227735965	50.36973780522137	16.229612807567424
18	15.98299581464312	15.43814868339558	48.36189260712074	20.216962894840563
19	14.820480096094293	19.1184474765864	44.87622229312513	21.18485013419418
20	17.324374542519845	18.615266229988176	51.44516807116983	12.615191156322142
21	16.623186501754848	24.8636474540643	45.52035434770369	12.992811696477169
22	14.912069968656743	13.714832679566824	50.21283384320865	21.16026350856778
23	15.295884086259642	22.961843809237813	47.8256789474672	13.91659315703534
24	16.88294138623524	17.418216625063344	45.02993562433137	20.668906364370038
25	14.04440607345958	27.787391377789454	42.17901315665997	15.989189392090989
26	14.871154820667781	15.656988419887014	48.0940673035416	21.377789455903606
27	18.1864079127644	18.198607383495055	43.2809068898857	20.334077813854844
28	11.56096920102851	20.673973836827386	48.736322516469286	19.028734445674818
29	18.23201516488054	19.690321127606463	43.21822037874664	18.85944332876635
30	14.608772357876166	21.861639233497872	45.67294157391941	17.856646834706556
31	19.694074810908205	16.039864116664475	41.17659203093035	23.08946904149697
32	23.78127287400762	21.42058144554344	39.38402057018449	15.414125110264449
33	16.292862371201743	20.397139693324075	37.93378502655731	25.376212908916873
34	19.10362042754453	23.421670013700943	41.20174170905201	16.27296784970252
35	16.20314934029016	22.426568570409714	35.93513635254594	25.43514573675419
36	17.39606989358308	29.204594508361332	32.61781873463336	20.781516863422233
37	20.942925245397046	21.028509224676714	36.7840318312344	21.244533698691843
38	19.337287213077833	26.556558623148963	30.653516262832902	23.4526379009403
39	23.769073403276966	22.205664308102325	36.27559542801374	17.749666860606972
40	19.82770593645014	24.207503612920178	33.90026463467277	22.06452581595691
41	18.058219628009986	27.339389275726806	34.279386648148495	20.323004448114713
42	19.708151123289728	20.67509994181791	38.057468891349636	21.559280043542724
43	21.007676282352058	25.292693455453165	31.72237758300332	21.977252679191455
44	22.402732681443666	20.734783506315573	34.348829789230685	22.51365402301008
45	18.696533473470843	19.731987012255775	34.28745706724723	27.284022447026146
46	24.90493797038344	24.153638257540212	31.114468572286558	19.826955199789793
47	15.653985473245621	22.465794560912897	42.564328747583566	19.315891218257917
48	20.20157279330343	24.355961787503986	32.857491413449445	22.584974005743135
49	18.77911450610912	19.73930669469417	38.841237964752914	22.640340834443798
50	19.307820799159174	21.273061691785063	35.480565304705245	23.93855220435052
51	18.931514048159755	20.45663557365665	33.24956363431617	27.36228674386742
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	52.0
1	772.0
2	1492.0
3	17690.0
4	33888.0
5	24479.5
6	15071.0
7	14254.5
8	13438.0
9	13054.5
10	12671.0
11	12032.0
12	11393.0
13	10621.0
14	9849.0
15	9201.0
16	8553.0
17	7796.5
18	7040.0
19	6486.0
20	5932.0
21	5685.0
22	5438.0
23	5179.0
24	4920.0
25	4968.0
26	5465.5
27	5915.0
28	6393.0
29	6871.0
30	7594.5
31	8318.0
32	8829.5
33	9341.0
34	9836.0
35	10331.0
36	10606.0
37	10881.0
38	11393.0
39	11905.0
40	12758.5
41	13612.0
42	14335.5
43	15059.0
44	17199.0
45	19339.0
46	56235.0
47	93131.0
48	63808.0
49	34485.0
50	31414.0
51	28343.0
52	25442.0
53	22541.0
54	22440.0
55	22339.0
56	21624.5
57	20910.0
58	19257.0
59	17604.0
60	15434.0
61	13264.0
62	11875.0
63	10486.0
64	8965.0
65	7444.0
66	6307.5
67	5171.0
68	4442.5
69	3714.0
70	3111.0
71	2508.0
72	2114.5
73	1721.0
74	1527.0
75	1038.5
76	744.0
77	565.0
78	386.0
79	278.0
80	170.0
81	135.5
82	101.0
83	80.5
84	60.0
85	38.0
86	16.0
87	12.0
88	8.0
89	5.0
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	532810.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.63971881318345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.67522816700577	34.42573253689905
2	5.640936535131695	4.697740223317943
3	2.4166175883179517	3.0188183056965907
4	1.5946930912548143	2.6561028765270707
5	1.2764291517799213	2.6575075482533093
6	1.0706145068448543	2.6748052213400877
7	0.9315195452447642	2.7151748353083547
8	0.7850142355227164	2.615021762520966
9	0.6359644736370053	2.3833243671677207
>10	2.8268265140993165	17.633388120074095
>50	0.09226109567057021	2.6559259763760448
>100	0.04658728593256396	3.657627524352331
>500	0.0018269523895123126	0.49524033243622456
>1k	0.003653904779024625	4.519163125710279
>5k	0.0	0.0
>10k+	0.0018269523895123126	13.194427244019952
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCG	19612	3.6808618456860795	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17109	3.2110883804733397	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGC	16423	3.082337043223663	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC	16233	3.046677051857135	No Hit
GAACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCT	4213	0.7907133875114957	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTC	3882	0.7285899288677014	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCGATCAT	3771	0.7077569865430453	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCGATCATCGTA	3378	0.6339971096638576	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCT	3159	0.5928942775098065	No Hit
GCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC	3068	0.5758150184868902	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCGATCATCGT	1283	0.24079878380661024	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTT	1008	0.18918563840768754	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCGATCATCGTATG	808	0.15164880539028922	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACCGATCATCGTAT	713	0.13381880970702503	No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	562	0.10547850077888929	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09571892419436571	0.0
2	0.0	0.0	0.0	0.78358138923819	0.0
3	0.0	0.0	0.0	0.8761096826260768	0.0
4	0.0	0.0	0.0	1.8237270321502974	0.0
5	0.0	0.0	0.0	5.386535537996659	0.0
6	0.0	0.0	0.0	5.6855164129802365	0.0
7	0.0	0.0	0.0	6.437379178318725	0.0
8	0.0	0.0	0.0	7.354028640603592	0.0
9	0.0	0.0	0.0	7.47583566374505	0.0
10	0.0	0.0	0.0	11.83048366209343	0.0
11	0.0	0.0	0.0	12.052326345226254	0.0
12	0.0	0.0	0.0	15.830783956757568	0.0
13	0.0	0.0	0.0	16.12845104258554	0.0
14	0.0	0.0	0.0	16.335654360841577	0.0
15	0.0	0.0	0.0	17.156772583097165	0.0
16	0.0	0.0	0.0	17.523507441677147	0.0
17	0.0	0.0	0.0	17.69167245359509	0.0
18	0.0	0.0	0.0	17.823051369155984	0.0
19	0.0	0.0	0.0	18.81083313000882	0.0
20	0.0	0.0	0.0	19.011467502486816	0.0
21	0.0	0.0	0.0	19.16161483455641	0.0
22	0.0	0.0	0.0	19.461158762035247	0.0
23	0.0	0.0	0.0	19.5974174658884	0.0
24	0.0	0.0	0.0	19.722039751506166	0.0
25	0.0	0.0	0.0	19.825078358138924	0.0
26	0.0	0.0	0.0	19.97635179519904	0.0
27	0.0	0.0	0.0	20.188997954242602	0.0
28	0.0	0.0	0.0	20.299356243313753	0.0
29	0.0	0.0	0.0	20.439556314633734	0.0
30	0.0	0.0	0.0	20.597211013306808	0.0
31	0.0	0.0	0.0	20.733469717159963	0.0
32	0.0	0.0	0.0	20.891687468328296	0.0
33	0.0	0.0	0.0	21.02269101555902	0.0
34	0.0	0.0	0.0	21.230832754640492	0.0
35	0.0	0.0	0.0	21.415138604755917	0.0
36	0.0	0.0	0.0	21.564535200165164	0.0
37	0.0	0.0	0.0	21.728383476286105	0.0
38	0.0	0.0	0.0	21.93539911037706	0.0
39	0.0	0.0	0.0	22.292186708207428	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATCG	25	3.8879793E-5	45.0	20
GTTAGCG	20	7.029877E-4	45.0	1
AGGCGAG	25	3.8879793E-5	45.0	45
GACGAAT	25	3.8879793E-5	45.0	20
GGCCTAA	20	7.029877E-4	45.0	8
CCGTTTG	25	3.8879793E-5	45.0	1
AAGTGCG	20	7.029877E-4	45.0	12
CGTTTTT	12800	0.0	44.279297	1
CGATGAA	525	0.0	43.714287	19
ACGGCTG	1915	0.0	42.532635	8
CCGATGA	545	0.0	42.522934	18
TGATACC	2355	0.0	42.42038	4
GATACCT	2365	0.0	42.33615	5
ATACCTG	2405	0.0	41.819126	6
TACGGCT	1950	0.0	41.769234	7
CGGCTGT	1995	0.0	41.61654	9
GAATGAC	405	0.0	40.555553	1
TACCTGT	2475	0.0	40.545456	7
CGGTGAA	100	0.0	40.5	19
CACCGGT	150	0.0	40.5	16
>>END_MODULE