##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933367.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340784
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.209229306540212	31.0	26.0	31.0	16.0	33.0
2	28.269634137752945	31.0	28.0	33.0	16.0	34.0
3	28.44024073900183	31.0	28.0	33.0	16.0	34.0
4	31.67613209540354	35.0	28.0	37.0	19.0	37.0
5	33.53113409080238	35.0	33.0	37.0	28.0	37.0
6	32.24373503450866	35.0	32.0	37.0	17.0	37.0
7	32.00498262829241	35.0	31.0	35.0	25.0	37.0
8	32.53801821681769	35.0	32.0	35.0	25.0	37.0
9	32.78238121508052	35.0	31.0	37.0	25.0	39.0
10	31.36611167190948	35.0	27.0	39.0	15.0	39.0
11	31.97088478332316	35.0	27.0	39.0	15.0	39.0
12	32.85881379407484	35.0	31.0	39.0	21.0	39.0
13	33.00780259636603	35.0	31.0	39.0	22.0	39.0
14	33.261705361754075	36.0	31.0	40.0	19.0	41.0
15	33.7149954223203	36.0	31.0	40.0	22.0	41.0
16	33.997623127846374	36.0	32.0	40.0	24.0	41.0
17	31.976768275505894	34.0	27.0	39.0	18.0	41.0
18	32.711430114089865	35.0	30.0	39.0	21.0	39.0
19	32.48154842950373	35.0	30.0	37.0	19.0	39.0
20	32.0075707779708	34.0	30.0	36.0	23.0	39.0
21	32.46500129114043	35.0	31.0	37.0	23.0	39.0
22	32.78234600215973	34.0	30.0	37.0	25.0	39.0
23	33.73995551434339	35.0	32.0	38.0	26.0	40.0
24	32.18532853655101	34.0	31.0	37.0	21.0	39.0
25	31.363549931921685	34.0	29.0	37.0	18.0	39.0
26	30.68661087374994	33.0	25.0	37.0	19.0	39.0
27	32.30401075167848	34.0	30.0	37.0	22.0	39.0
28	32.22841154514296	35.0	31.0	37.0	21.0	39.0
29	32.38439011221184	35.0	30.0	38.0	21.0	40.0
30	31.460171252171463	34.0	29.0	37.0	18.0	40.0
31	31.825176651485986	35.0	30.0	37.0	21.0	39.0
32	31.6289673224095	35.0	30.0	37.0	18.0	40.0
33	31.499239987792855	35.0	30.0	38.0	15.0	40.0
34	30.85713824592704	35.0	29.0	38.0	12.0	40.0
35	30.366205573031596	35.0	27.0	38.0	10.0	40.0
36	29.85997288605099	35.0	25.0	38.0	8.0	40.0
37	29.951012958354852	35.0	25.0	38.0	8.0	40.0
38	29.66374595051411	35.0	24.0	38.0	8.0	40.0
39	29.291501361566272	34.0	23.0	37.0	8.0	40.0
40	28.99681909948824	34.0	23.0	37.0	8.0	40.0
41	28.34498391943284	33.0	21.0	37.0	7.0	40.0
42	29.025852152683225	34.0	23.0	37.0	7.0	40.0
43	28.930369383539134	34.0	22.0	37.0	7.0	40.0
44	29.03905406357106	34.0	23.0	38.0	7.0	40.0
45	29.28360779848819	34.0	23.0	38.0	7.0	40.0
46	28.931505000234754	34.0	23.0	38.0	7.0	40.0
47	28.915844053711442	34.0	23.0	37.0	7.0	40.0
48	28.749369101835768	34.0	23.0	37.0	7.0	40.0
49	28.668420465749566	34.0	23.0	37.0	7.0	39.0
50	28.800694868303676	34.0	23.0	37.0	7.0	40.0
51	27.067274285177707	31.0	20.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	18.0
10	22.0
11	27.0
12	43.0
13	48.0
14	67.0
15	171.0
16	377.0
17	739.0
18	1279.0
19	2033.0
20	3082.0
21	4169.0
22	5783.0
23	7531.0
24	10484.0
25	14040.0
26	17530.0
27	19249.0
28	19731.0
29	20888.0
30	23281.0
31	26008.0
32	28890.0
33	31323.0
34	33464.0
35	33970.0
36	22749.0
37	11454.0
38	2289.0
39	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.469505610592048	2.3346166486689515	33.12156674022255	39.07431100051645
2	39.53325273487018	14.839311704774872	32.096870745105406	13.530564815249543
3	12.49383773886098	14.829628151556411	59.41094652331096	13.265587586271657
4	11.48087938400864	2.784755152824076	69.81929902812338	15.915066435043897
5	21.192602939105125	3.3326095121836707	59.31411099112635	16.160676557584864
6	13.441945631250293	13.073970608948777	61.274883797361376	12.209199962439552
7	50.38910277477816	1.4548805108221043	45.23217052443776	2.9238461899619703
8	50.889126250058695	10.239037043992676	34.6841401004742	4.187696605474436
9	47.27305272548007	2.6571083149443635	34.709376027043525	15.360462932532043
10	29.24814545283816	17.019284942955068	40.050589229541295	13.681980374665478
11	24.57421709939434	15.074944833090756	46.937062772900134	13.413775294614771
12	19.170794403493122	13.941675665524203	49.1810061505235	17.706523780459175
13	17.346471665336402	15.276538804638715	55.5715643926945	11.805425137330392
14	14.854864078125734	18.34182355979154	49.652565848161885	17.150746513920843
15	11.558347809756327	14.427613972486972	57.521186440677965	16.492851777078734
16	14.464294098314475	15.261866754307714	47.685043898774595	22.588795248603223
17	15.084628386309214	16.387213014695526	52.0042020752148	16.52395652378046
18	15.583478097563267	14.280013146157097	50.72245175829851	19.414056997981124
19	15.20816705009625	18.029602328747828	47.40510117845908	19.35712944269684
20	16.462920794403495	17.904889900934315	52.593431616507814	13.038757688154373
21	16.750199539884502	21.786820977510683	47.68298981172825	13.779989670876565
22	14.854277196112495	14.161169538475985	51.23597351988356	19.74857974552796
23	15.00980092962111	21.05439222498709	49.092386966524245	14.843419878867554
24	15.564404432132964	16.769273205314803	47.9723226442556	19.693999718296634
25	14.30407530869994	22.79567115827034	45.47983473402507	17.420418799004647
26	14.544403493121743	16.346131273768723	49.56247945912954	19.54698577398
27	15.664467815390392	17.494659373679518	46.87602704352317	19.96484576740692
28	11.677778299450678	19.175196018592423	50.781139959622514	18.365885722334383
29	17.021045588994788	18.01639748344993	46.381578947368425	18.580977980186862
30	14.612188365650969	20.150007042584157	47.88986572139537	17.3479388703695
31	18.298394290811775	16.492264895065496	44.503263063993614	20.706077750129115
32	20.807608338419644	20.3909221090192	42.92983238649702	15.871637166064135
33	15.696159444105357	19.085989952579933	42.03601108033241	23.1818395229823
34	17.478226677308793	22.9884618996197	42.82859523921311	16.704716183858398
35	15.139208413540542	22.46202873374337	39.121555002582284	23.27720785013381
36	17.013709563829288	25.821634818536083	36.29366402178506	20.87099159584957
37	19.636191839992488	21.07229212639091	38.94020846049111	20.3513075731255
38	18.802819381191604	24.89054650453073	35.2510681252641	21.05556598901357
39	21.238379736137848	21.823501103338184	39.390640405652846	17.547478754871122
40	18.994729799521103	22.915395088971312	37.20245081928729	20.88742429222029
41	17.24934269214517	25.123832104793653	37.587738860979385	20.039086342081788
42	18.64230715057045	20.597504577679704	39.6697028029485	21.09048546880135
43	19.14731912296352	23.7810460585004	35.66041832949904	21.411216489037045
44	20.894173435372554	20.408528569416404	37.091823559791536	21.605474435419502
45	17.968566599370863	19.9366754307714	37.24235879618762	24.852399173670126
46	21.88013521761585	23.053899244095966	35.17183905347669	19.894126484811494
47	15.5121719329546	21.68763791727311	43.50908493356496	19.29110521620733
48	18.951593971547958	22.938576928494296	36.96329639889196	21.14653270106578
49	17.794556082445183	19.693706277290012	40.14713132071928	22.36460631954552
50	18.261714164984273	20.697861401943754	37.70042020752148	23.340004225550494
51	18.132893563078078	19.877693788440773	36.57683459317339	25.412578055307762
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	77.0
1	352.0
2	627.0
3	17280.0
4	33933.0
5	22062.0
6	10191.0
7	9469.5
8	8748.0
9	8516.5
10	8285.0
11	7795.5
12	7306.0
13	6844.0
14	6382.0
15	5850.0
16	5318.0
17	4784.0
18	4250.0
19	3923.5
20	3597.0
21	3420.5
22	3244.0
23	3134.0
24	3024.0
25	3030.5
26	3199.0
27	3361.0
28	3666.5
29	3972.0
30	4475.0
31	4978.0
32	5243.0
33	5508.0
34	5985.5
35	6463.0
36	6655.0
37	6847.0
38	7212.0
39	7577.0
40	8119.5
41	8662.0
42	9375.5
43	10089.0
44	11397.5
45	12706.0
46	26796.0
47	40886.0
48	33137.5
49	25389.0
50	23266.0
51	21143.0
52	18625.5
53	16108.0
54	15083.5
55	14059.0
56	13419.5
57	12780.0
58	11722.5
59	10665.0
60	9467.0
61	8269.0
62	7485.5
63	6702.0
64	5959.0
65	5216.0
66	4387.5
67	3559.0
68	3097.5
69	2636.0
70	2214.5
71	1793.0
72	1525.0
73	1257.0
74	1116.5
75	779.5
76	583.0
77	438.0
78	293.0
79	228.0
80	163.0
81	115.0
82	67.0
83	51.0
84	35.0
85	25.5
86	16.0
87	9.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	340784.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.23354052915393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7013322687221	36.504069839339586
2	6.819965245846045	6.169823487107966
3	3.270598000652431	4.4382218165124465
4	2.1372134912448764	3.866949323027187
5	1.6399389079202173	3.709012152837279
6	1.193210364900445	3.2383877640318497
7	0.9435010914168769	2.987452640053438
8	0.7205917618664612	2.607593333228881
9	0.5598571708441354	2.2791889825124088
>10	1.8949880159039985	12.59916352477222
>50	0.06798210616827179	2.153744150701651
>100	0.043561349583552796	3.68560736628942
>500	0.001320040896471297	0.43767520445364844
>1k	0.0039601226894138905	3.490653813418866
>5k	0.001320040896471297	4.578572261596966
>10k+	6.600204482356485E-4	7.253884340116164
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24297	7.129736137846847	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCG	9016	2.6456641156861824	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGC	6320	1.854547161838584	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC	4781	1.4029414526503592	No Hit
GAACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCT	1666	0.4888727170289685	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCGATCAT	1650	0.48417766092304804	No Hit
GCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC	1403	0.4116977322879008	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTC	1164	0.34156533170571385	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCGATCATCGTA	1028	0.30165735480538997	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCT	804	0.2359265693225034	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCACCGATCATCGTATGCCG	662	0.19425794638245927	No Hit
GAATCTTTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC	424	0.12441898680689235	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCGATCATCGT	407	0.11943048969435185	No Hit
GAATGACTCTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCT	368	0.10798629043617071	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11825672566787174	0.0
2	0.0	0.0	0.0	0.8157659984036809	0.0
3	0.0	0.0	0.0	1.018240292971501	0.0
4	0.0	0.0	0.0	1.8601225409643645	0.0
5	0.0	0.0	0.0	3.7569252077562325	0.0
6	0.0	0.0	0.0	4.108760974693648	0.0
7	0.0	0.0	0.0	4.591471430583596	0.0
8	0.0	0.0	0.0	5.261397248697122	0.0
9	0.0	0.0	0.0	5.449492933940561	0.0
10	0.0	0.0	0.0	8.8754166862294	0.0
11	0.0	0.0	0.0	9.511009906568383	0.0
12	0.0	0.0	0.0	12.19599511714165	0.0
13	0.0	0.0	0.0	12.485327949669	0.0
14	0.0	0.0	0.0	12.650535236396076	0.0
15	0.0	0.0	0.0	13.188999483543828	0.0
16	0.0	0.0	0.0	13.511491149819241	0.0
17	0.0	0.0	0.0	13.797889572280388	0.0
18	0.0	0.0	0.0	14.074604441523077	0.0
19	0.0	0.0	0.0	14.907096577304099	0.0
20	0.0	0.0	0.0	15.202591670970468	0.0
21	0.0	0.0	0.0	15.541809474623221	0.0
22	0.0	0.0	0.0	16.02305272548007	0.0
23	0.0	0.0	0.0	16.3267641673318	0.0
24	0.0	0.0	0.0	16.569146438799944	0.0
25	0.0	0.0	0.0	16.786586224705385	0.0
26	0.0	0.0	0.0	16.979963848067985	0.0
27	0.0	0.0	0.0	17.207674069205126	0.0
28	0.0	0.0	0.0	17.398704164514765	0.0
29	0.0	0.0	0.0	17.63110944175783	0.0
30	0.0	0.0	0.0	17.908704634020378	0.0
31	0.0	0.0	0.0	18.115580543687496	0.0
32	0.0	0.0	0.0	18.324510540400958	0.0
33	0.0	0.0	0.0	18.532853655101178	0.0
34	0.0	0.0	0.0	18.771714634489882	0.0
35	0.0	0.0	0.0	19.024073900183108	0.0
36	0.0	0.0	0.0	19.234471101929667	0.0
37	0.0	0.0	0.0	19.426088079252548	0.0
38	0.0	0.0	0.0	19.68196863702521	0.0
39	0.0	0.0	0.0	20.15470209869008	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGA	25	3.8850092E-5	45.000004	4
TCGGAAT	25	3.8850092E-5	45.000004	24
TCTCACG	20	7.026293E-4	45.000004	28
GTTATAC	25	3.8850092E-5	45.000004	33
TAGTGGG	30	2.1609903E-6	44.999996	3
CGTTTTT	9355	0.0	44.326565	1
TCAAGCG	120	0.0	43.124996	17
CACGACC	110	0.0	42.954544	27
ACGACCA	110	0.0	42.954544	28
AATGACT	105	0.0	42.857143	2
ACCGGTG	90	0.0	42.5	17
TACGGCT	1035	0.0	42.3913	7
GATACCT	1165	0.0	42.29614	5
GAATGAT	3450	0.0	42.130432	1
CGATGAA	150	0.0	42.0	19
TGATACC	1180	0.0	41.758472	4
ACGGCTG	1055	0.0	41.587677	8
AATGATA	3315	0.0	41.47059	2
CACCGGT	125	0.0	41.399998	16
ATACGGC	2180	0.0	41.181194	6
>>END_MODULE