##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933366.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	366007
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.015879477714908	31.0	28.0	31.0	25.0	34.0
2	27.71807643023221	31.0	25.0	33.0	16.0	34.0
3	27.689385722130996	31.0	25.0	33.0	16.0	34.0
4	32.749037586712824	35.0	32.0	37.0	28.0	37.0
5	34.12858224023038	35.0	33.0	37.0	30.0	37.0
6	33.05197168360168	35.0	32.0	37.0	28.0	37.0
7	33.648034053993506	35.0	33.0	37.0	29.0	37.0
8	33.0743701623193	35.0	32.0	37.0	27.0	37.0
9	34.72270748920103	37.0	34.0	39.0	28.0	39.0
10	34.688923982328205	37.0	33.0	39.0	28.0	39.0
11	35.056310398435	37.0	34.0	39.0	28.0	39.0
12	35.13364771712016	37.0	34.0	39.0	29.0	39.0
13	35.15577844139593	37.0	34.0	39.0	29.0	39.0
14	35.98841005773114	38.0	34.0	40.0	29.0	41.0
15	36.08127166966752	38.0	34.0	40.0	27.0	41.0
16	36.20222017611685	38.0	34.0	40.0	30.0	41.0
17	35.32090096637496	37.0	33.0	40.0	27.0	41.0
18	35.11209075236266	37.0	33.0	39.0	27.0	40.0
19	34.45586559819894	37.0	33.0	37.0	27.0	40.0
20	33.31269620526383	35.0	32.0	37.0	25.0	39.0
21	34.096566459111436	35.0	33.0	38.0	27.0	40.0
22	34.610791596882024	35.0	33.0	39.0	28.0	40.0
23	34.71918843082236	35.0	33.0	39.0	28.0	40.0
24	34.413150568158535	35.0	33.0	38.0	27.0	40.0
25	33.500304638982314	35.0	33.0	38.0	24.0	40.0
26	33.38946796099528	35.0	32.0	38.0	24.0	40.0
27	34.057936050403406	35.0	33.0	38.0	25.0	40.0
28	33.55140475455387	35.0	33.0	38.0	24.0	40.0
29	33.45134382675741	35.0	33.0	38.0	23.0	40.0
30	32.95349542495089	35.0	32.0	38.0	21.0	40.0
31	33.13045652132336	35.0	33.0	38.0	21.0	40.0
32	32.42039633121771	35.0	31.0	38.0	17.0	40.0
33	31.59466622223072	35.0	30.0	39.0	12.0	40.0
34	30.943818014409562	35.0	28.0	39.0	10.0	40.0
35	30.561169595117033	35.0	25.0	39.0	8.0	40.0
36	30.059302144494506	35.0	23.0	39.0	7.0	40.0
37	29.938195171130605	35.0	23.0	39.0	7.0	40.0
38	29.693880171690704	35.0	22.0	39.0	7.0	40.0
39	29.34528301371285	35.0	21.0	38.0	7.0	40.0
40	28.961754829825658	35.0	21.0	38.0	7.0	40.0
41	28.713822413232535	35.0	20.0	38.0	7.0	40.0
42	28.908370604933786	35.0	19.0	39.0	7.0	40.0
43	28.73924269207966	35.0	18.0	39.0	7.0	40.0
44	28.894193280456385	35.0	18.0	39.0	7.0	40.0
45	28.98973790118768	35.0	20.0	39.0	7.0	40.0
46	28.7021204512482	35.0	18.0	39.0	7.0	40.0
47	28.463941946465503	34.0	18.0	38.0	7.0	40.0
48	28.434759444491498	34.0	18.0	38.0	7.0	40.0
49	28.59824265656121	35.0	18.0	39.0	7.0	40.0
50	28.50129369110427	34.0	18.0	39.0	7.0	40.0
51	27.326236929894783	33.0	18.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	22.0
10	23.0
11	12.0
12	25.0
13	45.0
14	46.0
15	97.0
16	188.0
17	413.0
18	866.0
19	1530.0
20	2512.0
21	3702.0
22	5321.0
23	7321.0
24	10223.0
25	14649.0
26	19178.0
27	20570.0
28	19076.0
29	17565.0
30	17984.0
31	19878.0
32	22747.0
33	26198.0
34	29688.0
35	33533.0
36	34790.0
37	31629.0
38	22973.0
39	3198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.275573964432375	2.1166261847451002	24.61783517801572	38.989964672806806
2	36.905031870975144	25.586122669785006	26.592660796104994	10.916184663134858
3	9.947350733729136	25.78502596944867	53.33805091159459	10.929572385227605
4	9.24763734026945	2.3570587447781053	75.16577551795457	13.229528396997873
5	27.00658730570727	2.8384703024805535	54.17983809052832	15.975104301283855
6	11.154158253803889	21.724174674254865	58.13358760898015	8.98807946296109
7	42.722680167319204	1.1939662356184444	53.051717590100736	3.0316360069616155
8	40.19841150579087	17.757037433710284	37.84709035619537	4.19746070430347
9	37.82113456846454	2.5004986243432503	36.020349337580974	23.658017469611238
10	29.68686391243883	14.063392230203247	42.392905053728484	13.85683880362944
11	25.148972560634085	12.748390058113642	47.96274388194761	14.139893499304659
12	17.08464592207253	11.276560284366147	50.266524957172955	21.372268836388375
13	17.08683167261828	12.851393552582326	59.257883045952674	10.803891728846716
14	14.194537262948522	22.544104347731054	46.422882622463504	16.838475766856916
15	9.494353933121499	13.284172160641736	61.60210050627447	15.619373399962297
16	10.803618510028496	15.906526377910804	46.972052447084344	26.31780266497635
17	11.19022313780884	17.146939812626535	55.02299136355315	16.63984568601147
18	11.03312231733273	15.787949410803618	51.94217596931207	21.23675230255159
19	11.717808675790353	18.462215203534356	48.42503012237471	21.394945998300578
20	14.875671776769298	17.0078714341529	56.18198559043953	11.934471198638278
21	14.503547746354577	24.889687902143947	47.897991022029636	12.708773329471839
22	11.952230421822534	13.412038567568379	53.15608717866051	21.479643831948568
23	13.537992442767488	23.187534664637564	49.69221900127593	13.582253891319018
24	15.953520014644528	16.04586797520266	46.33818478881551	21.662427221337296
25	10.808809667574664	28.659834374752396	45.10323573046417	15.428120227208769
26	12.01015281128503	14.82130123194365	52.275776146357856	20.892769810413462
27	16.79776616294224	19.52831503222616	44.959522632080805	18.714396172750796
28	10.28942069413973	19.02777815724836	53.16728915020751	17.515511998404403
29	17.260872059823992	18.510301715540958	46.37506932927513	17.853756895359922
30	11.591035144136587	26.20223110486958	48.64633736513236	13.560396385861473
31	16.97481195714836	15.671011756605749	42.61749092230476	24.736685363941127
32	23.360482176570393	22.4372757898073	41.05030778099872	13.151934252623585
33	14.75381618384348	16.63957246719325	43.70271606827192	24.90389528069135
34	19.91874472346157	23.04710019207283	41.80985609564844	15.224298988817154
35	19.846888174269893	23.856374331638467	37.600920200979765	18.69581729311188
36	19.0835147961651	23.821129104088172	35.94330163084313	21.1520544689036
37	20.311086946424524	24.64187843401902	40.01344236585639	15.033592253700064
38	18.06167641602483	20.502066900359832	37.340542667216745	24.095714016398592
39	23.614575677514367	21.320630479744924	39.532304026972156	15.532489815768551
40	20.322835355607953	21.404508656938255	41.816686566103925	16.455969421349863
41	21.263254527918864	23.26922709128514	37.78780187264178	17.67971650815422
42	21.512703308953107	20.423106661894444	41.04211121645214	17.022078812700304
43	24.490788427543738	21.034570377069294	33.350728264759965	21.123912930627007
44	19.91328034709719	21.945208698194296	37.05229681399536	21.089214140713157
45	16.54749772545334	19.816834104265766	36.33263844680566	27.303029723475237
46	26.980904736794653	23.76238705817102	31.92999041001948	17.326717795014847
47	14.592343862275857	19.83131470163139	47.28762018212767	18.288721253965086
48	19.67421388115528	22.814864196586406	34.047163032401016	23.463758889857296
49	17.818511667809634	18.156483345946935	42.82049250424172	21.20451248200171
50	20.927195381509097	17.984901928105202	38.069217255407686	23.01868543497802
51	18.532159220998505	19.247992524733135	35.44057900531957	26.77926924894879
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	25.0
1	569.5
2	1114.0
3	12853.5
4	24593.0
5	18603.5
6	12614.0
7	12229.0
8	11844.0
9	11669.5
10	11495.0
11	10924.0
12	10353.0
13	9558.5
14	8764.0
15	8013.5
16	7263.0
17	6689.0
18	6115.0
19	5569.0
20	5023.0
21	4722.5
22	4422.0
23	4170.5
24	3919.0
25	3804.0
26	3648.5
27	3608.0
28	3678.5
29	3749.0
30	3753.0
31	3757.0
32	4156.0
33	4555.0
34	4894.0
35	5233.0
36	5635.5
37	6038.0
38	6617.5
39	7197.0
40	7904.0
41	8611.0
42	9414.0
43	10217.0
44	11961.0
45	13705.0
46	45590.0
47	77475.0
48	51368.0
49	25261.0
50	22226.5
51	19192.0
52	16074.0
53	12956.0
54	11772.5
55	10589.0
56	10209.5
57	9830.0
58	9011.5
59	8193.0
60	7550.0
61	6907.0
62	6243.5
63	5580.0
64	4889.5
65	4199.0
66	3364.0
67	2529.0
68	2192.0
69	1855.0
70	1546.0
71	1237.0
72	1087.0
73	937.0
74	764.0
75	485.5
76	380.0
77	282.5
78	185.0
79	153.0
80	121.0
81	87.5
82	54.0
83	37.0
84	20.0
85	12.5
86	5.0
87	5.5
88	6.0
89	3.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	366007.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.29560578508441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.43700078579928	37.84483706267712
2	4.939310182218657	4.375794733636221
3	2.1052640309450945	2.79761836764785
4	1.3625323355168466	2.4141678081393843
5	1.0580488161922348	2.3433456631713234
6	0.9190121282851859	2.442491936773921
7	0.7416973792145911	2.299775430506386
8	0.611636225340872	2.167423769726106
9	0.5041765323224715	2.0099524429662363
>10	2.2155586913705108	14.949384765033605
>50	0.06096919702259664	1.8193680553996776
>100	0.036083810482761276	3.0523054501070632
>500	0.0018664039904876522	0.6407195893372085
>1k	0.004354942644471189	5.196855920740249
>5k	0.0	0.0
>10k+	0.0024885386539835364	15.64595900413764
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCG	16222	4.432155669153868	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGC	15365	4.198007141939908	No Hit
GAATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC	14017	3.8297081749802597	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11171	3.0521274183280647	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAACGTTGGT	3446	0.9415120475837894	No Hit
GAACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT	3138	0.8573606515722377	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTC	2944	0.8043562008376889	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTA	2943	0.8040829820194696	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCT	2685	0.7335925269188841	No Hit
GCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	2516	0.6874185466398184	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGT	1186	0.3240375184081179	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTAT	804	0.21966792984833622	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATG	764	0.2087391771195633	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTT	757	0.20682664539202802	No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT	445	0.12158237410759903	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	406	0.11092684019704542	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAACGTTGG	397	0.1084678708330715	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12868606338130145	0.0
2	0.0	0.0	0.0	0.9587248331316067	0.0
3	0.0	0.0	0.0	1.0854983647853729	0.0
4	0.0	0.0	0.0	2.137937252566208	0.0
5	0.0	0.0	0.0	6.618179433726677	0.0
6	0.0	0.0	0.0	6.9602493941372705	0.0
7	0.0	0.0	0.0	7.8809968115363915	0.0
8	0.0	0.0	0.0	8.89982978467625	0.0
9	0.0	0.0	0.0	9.051466228787975	0.0
10	0.0	0.0	0.0	14.330873453239965	0.0
11	0.0	0.0	0.0	14.644801875373968	0.0
12	0.0	0.0	0.0	19.727491550708045	0.0
13	0.0	0.0	0.0	20.1171015854888	0.0
14	0.0	0.0	0.0	20.43758725926007	0.0
15	0.0	0.0	0.0	21.491392241132	0.0
16	0.0	0.0	0.0	22.003131087656794	0.0
17	0.0	0.0	0.0	22.225257986869103	0.0
18	2.7321881821932366E-4	0.0	0.0	22.385910651982066	0.0
19	2.7321881821932366E-4	0.0	0.0	23.72905436234826	0.0
20	2.7321881821932366E-4	0.0	0.0	23.99134442783881	0.0
21	2.7321881821932366E-4	0.0	0.0	24.151997092951774	0.0
22	2.7321881821932366E-4	0.0	0.0	24.528492624458003	0.0
23	2.7321881821932366E-4	0.0	0.0	24.673298598114243	0.0
24	2.7321881821932366E-4	0.0	0.0	24.822749291680214	0.0
25	2.7321881821932366E-4	0.0	0.0	24.927392099058213	0.0
26	2.7321881821932366E-4	0.0	0.0	25.105530768537214	0.0
27	2.7321881821932366E-4	0.0	0.0	25.33831320166008	0.0
28	2.7321881821932366E-4	0.0	0.0	25.45798304404014	0.0
29	2.7321881821932366E-4	0.0	0.0	25.60005682951419	0.0
30	2.7321881821932366E-4	0.0	0.0	25.7344804880781	0.0
31	2.7321881821932366E-4	0.0	0.0	25.879559680552557	0.0
32	2.7321881821932366E-4	0.0	0.0	26.042124877393054	0.0
33	2.7321881821932366E-4	0.0	0.0	26.177914630048058	0.0
34	2.7321881821932366E-4	0.0	0.0	26.392391402350228	0.0
35	2.7321881821932366E-4	0.0	0.0	26.551677973372094	0.0
36	2.7321881821932366E-4	0.0	0.0	26.684462319026686	0.0
37	2.7321881821932366E-4	0.0	0.0	26.848666828776498	0.0
38	2.7321881821932366E-4	0.0	0.0	27.007133743343708	0.0
39	2.7321881821932366E-4	0.0	0.0	27.168332846093108	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	20	7.026978E-4	45.000004	21
CTTCGTG	20	7.026978E-4	45.000004	1
CGACGAA	30	2.1614323E-6	45.000004	19
TAGTTGC	20	7.026978E-4	45.000004	14
TTATACG	20	7.026978E-4	45.000004	26
ACAGGCG	20	7.026978E-4	45.000004	33
TAGATTC	20	7.026978E-4	45.000004	19
CGGTAGC	20	7.026978E-4	45.000004	33
GATTCGC	20	7.026978E-4	45.000004	11
CCAGTAG	30	2.1614323E-6	45.000004	26
CGATCCA	20	7.026978E-4	45.000004	34
CGTCATA	20	7.026978E-4	45.000004	38
ACATTGA	25	3.8855786E-5	45.0	38
ACCGACG	25	3.8855786E-5	45.0	17
CAACCCG	25	3.8855786E-5	45.0	23
CGTTTTT	9970	0.0	44.435806	1
ACGGCTG	1815	0.0	43.76033	8
TACGGCT	1815	0.0	43.512398	7
CGATGAA	485	0.0	43.144333	19
GATACCT	1965	0.0	43.053432	5
>>END_MODULE