SRR2933365.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933365.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	231684
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15276	6.593463510643809	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCG	8123	3.5060686106938763	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGC	7002	3.022219920236184	No Hit
GAATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC	4907	2.117971029505706	No Hit
GCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	2142	0.9245351426943595	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAACGTTGGT	1924	0.8304414633725247	No Hit
GAACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT	1704	0.7354845392862692	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTA	1228	0.5300322853541893	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTC	1120	0.4834170680754821	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCT	788	0.3401184371816785	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAACGTTGGTCGTATGCCG	628	0.2710588560280382	No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT	585	0.2524990935929974	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGT	525	0.22660175066038227	No Hit
CCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	426	0.1838711348215673	No Hit
GAATCTTTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC	413	0.17826004385283403	No Hit
GAATGACTCTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCT	365	0.15754216950674194	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATG	349	0.1506362113913779	No Hit
TCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	348	0.15020458900916767	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTAT	320	0.1381191623072806	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGC	309	0.13337131610296785	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCC	306	0.13207644895633708	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTT	305	0.13164482657412682	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCAACGTTGGTCGTATGC	297	0.1281918475164448	No Hit
GAATCTGTCTCTTATCCACATCTGACGCAACGTTGGTCGTATGCCGTCTTC	286	0.12344400131213205	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	275	0.11869615510781927	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCG	265	0.11437993128571675	No Hit
GAATCTGTCTCTTATCCCCATCTGACGCAACGTTGGTCGTATGCCGTCTTC	259	0.11179019699245525	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	252	0.10876884031698349	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACCG	30	2.1579744E-6	45.000004	11
TAGGGAC	30	2.1579744E-6	45.000004	5
GCGAGAC	20	7.021615E-4	45.0	21
ACCGGTG	55	1.8189894E-12	45.0	17
GGGCTTA	20	7.021615E-4	45.0	7
GGCGTTT	20	7.021615E-4	45.0	7
TATGGGC	25	3.881134E-5	45.0	4
TATGGGA	45	3.8198777E-10	45.0	4
GATCCGT	20	7.021615E-4	45.0	5
ATACTTC	20	7.021615E-4	45.0	42
ATCCGTC	20	7.021615E-4	45.0	6
TTATACG	25	3.881134E-5	45.0	26
GTATGGC	20	7.021615E-4	45.0	9
GCCCTCG	20	7.021615E-4	45.0	20
GCGATTG	25	3.881134E-5	45.0	9
ATGACTC	50	2.1827873E-11	45.0	3
TCCGTCT	20	7.021615E-4	45.0	7
TGCTACG	95	0.0	45.0	4
CTCCTAT	50	2.1827873E-11	45.0	16
CACCGGT	65	0.0	44.999996	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content