##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933365.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 231684 Sequences flagged as poor quality 0 Sequence length 51 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.60595034616115 30.0 25.0 31.0 16.0 33.0 2 27.340619119145043 31.0 25.0 33.0 16.0 34.0 3 27.47875554634761 31.0 25.0 33.0 16.0 34.0 4 31.068558899190275 35.0 28.0 35.0 19.0 37.0 5 33.44802403273424 35.0 32.0 37.0 28.0 37.0 6 31.566305830355137 35.0 31.0 37.0 17.0 37.0 7 32.268836000759656 35.0 32.0 35.0 26.0 37.0 8 32.05015452081283 35.0 32.0 35.0 17.0 37.0 9 33.081257229674904 35.0 32.0 37.0 26.0 39.0 10 32.01124376305658 35.0 28.0 39.0 17.0 39.0 11 32.809209958391605 35.0 30.0 39.0 17.0 39.0 12 33.50117401287961 35.0 31.0 39.0 25.0 39.0 13 33.664059667478114 35.0 32.0 39.0 25.0 39.0 14 33.85198805269246 36.0 32.0 40.0 22.0 41.0 15 34.168967213963846 37.0 32.0 40.0 25.0 41.0 16 34.25524852816768 36.0 32.0 40.0 25.0 41.0 17 32.13322456449302 35.0 27.0 39.0 18.0 41.0 18 32.84356278379172 36.0 30.0 39.0 23.0 39.0 19 32.59051121354949 36.0 30.0 37.0 21.0 39.0 20 31.894826574126828 34.0 30.0 36.0 23.0 39.0 21 32.61253258748986 35.0 31.0 37.0 24.0 39.0 22 32.8380337010756 34.0 30.0 37.0 25.0 39.0 23 33.6184587627976 35.0 32.0 38.0 26.0 40.0 24 32.26950069922826 35.0 31.0 37.0 21.0 39.0 25 31.57683741648107 35.0 30.0 37.0 19.0 39.0 26 30.595669101016902 33.0 25.0 37.0 18.0 39.0 27 32.32006957752801 34.0 30.0 37.0 22.0 39.0 28 32.062835586402166 35.0 30.0 37.0 21.0 39.0 29 32.110633449008134 35.0 30.0 38.0 21.0 40.0 30 31.385926520605654 34.0 29.0 37.0 18.0 40.0 31 31.75250772604064 35.0 30.0 37.0 20.0 39.0 32 31.56439805942577 35.0 30.0 37.0 18.0 39.0 33 31.291379637782498 35.0 30.0 38.0 15.0 40.0 34 30.367690474957268 35.0 27.0 37.0 10.0 39.0 35 29.90364030317156 35.0 25.0 38.0 8.0 40.0 36 29.308048894183457 35.0 23.0 38.0 7.0 40.0 37 29.366775435506984 35.0 23.0 38.0 7.0 40.0 38 29.183249598591186 34.0 23.0 38.0 8.0 40.0 39 28.842531206298233 34.0 22.0 37.0 7.0 40.0 40 28.51732963864574 33.0 21.0 37.0 7.0 39.0 41 28.196228483624246 33.0 20.0 37.0 7.0 40.0 42 28.58141692995632 34.0 21.0 37.0 7.0 40.0 43 28.464067436680995 34.0 20.0 38.0 7.0 40.0 44 28.497155608501235 34.0 20.0 38.0 7.0 40.0 45 28.72610538492084 34.0 20.0 38.0 7.0 40.0 46 28.392482864591425 34.0 20.0 38.0 7.0 40.0 47 28.278668358626405 34.0 20.0 38.0 7.0 40.0 48 28.156657343623213 34.0 20.0 37.0 7.0 40.0 49 28.06657775245593 34.0 20.0 37.0 7.0 40.0 50 28.19192089224979 34.0 20.0 37.0 7.0 40.0 51 26.431549006405277 31.0 18.0 35.0 7.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 10.0 11 10.0 12 15.0 13 24.0 14 51.0 15 167.0 16 267.0 17 556.0 18 825.0 19 1499.0 20 2204.0 21 3033.0 22 4038.0 23 5491.0 24 7557.0 25 10375.0 26 12866.0 27 14118.0 28 13659.0 29 14032.0 30 15448.0 31 17212.0 32 19393.0 33 21194.0 34 22782.0 35 22260.0 36 14357.0 37 6875.0 38 1332.0 39 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.980490668324094 2.196957925450182 26.619015555670657 41.20353585055507 2 39.90780545915989 20.73427599661608 27.549593411715957 11.808325132508072 3 10.435334334697261 20.62939175773899 57.730788487767825 11.20448541979593 4 10.23117694791181 2.3829871721828004 73.59118454446573 13.794651335439651 5 23.764696742114257 2.817630911068524 57.99666787520934 15.42100447160788 6 11.629633466273027 17.174254588145924 60.258369157991055 10.937742787589993 7 41.887657326358315 1.2499784188808896 53.46679097391275 3.395573280848051 8 41.079660226860724 14.364392879957183 38.920253448662834 5.635693444519259 9 37.780338737245565 2.4214015641995132 38.84126655271836 20.95699314583657 10 27.28069266759897 14.217641270005698 43.95685502667426 14.54481103572107 11 22.72362355622313 13.588335836743152 49.99741026570674 13.690630341326978 12 16.7732773950726 11.535971409333403 52.123582120474445 19.56716907511956 13 16.079660226860724 13.868890385179814 59.2583000984099 10.793149289549559 14 13.356554617496244 19.815783567272664 49.0189223252361 17.80873948999499 15 9.16852264291017 14.165846584140468 60.94853334714525 15.717097425804113 16 10.181540373957631 14.169299563198148 49.19200290050241 26.45715716234181 17 10.755598142297266 16.358919908150757 55.763022047271285 17.122459902280692 18 10.635175497660606 14.454601957839126 53.78575991436612 21.124462630134147 19 11.51525353498731 17.722846635935152 50.04143574869219 20.720464080385355 20 13.956941351150705 15.979092211805735 57.870634139603936 12.193332297439616 21 14.951830942145335 22.228121061445762 48.90281590442154 13.91723209198736 22 11.49842026208111 14.257350529169042 53.66663213687609 20.57759707187376 23 12.954714179658499 21.996771464581066 49.923171215966576 15.125343139793856 24 15.177137825659088 15.148650748433209 48.434937242105626 21.239274183802078 25 10.93256331900347 24.312425545139067 46.996771464581066 17.758239671276392 26 11.71984254415497 16.209146941523798 52.656204140121886 19.41480637419934 27 14.08254346437389 19.489908668703922 47.53068835137515 18.89685951554704 28 10.097374009426632 18.212306417361578 54.26227102432624 17.42804854888555 29 16.680910205279606 17.174254588145924 48.2696258697191 17.87520933685537 30 11.343899449249841 23.437095354016677 51.03071424871808 14.1882909480154 31 16.195766647675285 15.717960670568534 45.72607517135408 22.3601975104021 32 21.051086825158404 21.482709207368657 43.96203449526079 13.504169472212151 33 14.281521382572816 16.00542117712056 46.26085530291259 23.45220213739404 34 18.81226152863383 22.505654253206956 43.5541513440721 15.127932874087119 35 17.647312719048358 23.26703613542584 40.03729217382296 19.048358971702836 36 17.947290274684484 22.200497228984307 38.716959306641805 21.135253189689404 37 19.318122960584244 23.54111634812935 41.67141451287098 15.469346178415428 38 17.944700540391224 19.763125636643014 40.1439029022289 22.148270920736866 39 21.47062378066677 20.140795221076985 43.106558933720066 15.28202206453618 40 20.073893751834397 20.18697881597348 43.53947618307695 16.199651249115174 41 20.605220904335216 21.243590407624175 40.41409851349251 17.737090174548094 42 21.28114155487647 19.159717546313082 41.919165760259666 17.639975138550785 43 23.34041194040158 19.838227931147596 36.235562231315065 20.585797897135755 44 19.046200859791785 21.002313495968647 39.66307556844668 20.28841007579289 45 16.59026950501545 18.76737280088396 38.48345159786606 26.158906096234524 46 24.52089915574662 22.47932528789213 35.70768805787193 17.292087498489323 47 14.520640182317294 18.681048324441914 48.33350598228622 18.464805510954577 48 18.95038069094111 21.047633846100723 37.474750090640704 22.527235372317467 49 17.90758101552114 16.926071718375027 44.0345470554721 21.131800210631724 50 20.795566374889937 16.604081421246182 39.862485109027816 22.73786709483607 51 18.190725298251063 17.6658724814834 38.454101275875765 25.68930094438977 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 39.0 1 225.0 2 411.0 3 11585.5 4 22760.0 5 15451.0 6 8142.0 7 7786.5 8 7431.0 9 7294.5 10 7158.0 11 6748.5 12 6339.0 13 5795.5 14 5252.0 15 4810.0 16 4368.0 17 3956.5 18 3545.0 19 3200.0 20 2855.0 21 2659.5 22 2464.0 23 2295.0 24 2126.0 25 2081.0 26 1990.5 27 1945.0 28 2006.5 29 2068.0 30 2065.5 31 2063.0 32 2321.5 33 2580.0 34 2737.5 35 2895.0 36 3187.0 37 3479.0 38 3842.0 39 4205.0 40 4794.0 41 5383.0 42 6020.5 43 6658.0 44 7851.5 45 9045.0 46 23144.5 47 37244.0 48 28743.0 49 20242.0 50 18197.5 51 16153.0 52 12804.5 53 9456.0 54 8215.0 55 6974.0 56 6494.0 57 6014.0 58 5511.5 59 5009.0 60 4618.0 61 4227.0 62 3869.0 63 3511.0 64 2994.5 65 2478.0 66 2021.5 67 1565.0 68 1371.5 69 1178.0 70 985.0 71 792.0 72 724.0 73 656.0 74 518.5 75 336.5 76 292.0 77 208.0 78 124.0 79 100.0 80 76.0 81 57.0 82 38.0 83 26.0 84 14.0 85 10.0 86 6.0 87 5.5 88 5.0 89 3.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 231684.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.657466449046034 #Duplication Level Percentage of deduplicated Percentage of total 1 82.02865534170896 36.63191923782729 2 6.9234615175173 6.183685008595804 3 3.0560631109200527 4.094281075262385 4 2.0272411254985228 3.6212581018430647 5 1.3614498671050166 3.0399450881150236 6 1.087558105757399 2.9140553771549467 7 0.7896790062259049 2.4685544608234555 8 0.6047541679825205 2.1605431173280127 9 0.46681024161677453 1.8761906432764965 >10 1.531377494865812 10.321057306653675 >50 0.06297440089843855 1.9971632018478624 >100 0.046980902257565266 4.178686797607922 >500 0.003998374660218321 1.127589237341909 >1k 0.005997561990327481 5.814271502920968 >5k 0.0019991873301091604 6.7516972346778985 >10k+ 9.995936650545802E-4 6.819102608723278 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15276 6.593463510643809 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCG 8123 3.5060686106938763 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGC 7002 3.022219920236184 No Hit GAATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC 4907 2.117971029505706 No Hit GCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC 2142 0.9245351426943595 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAACGTTGGT 1924 0.8304414633725247 No Hit GAACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT 1704 0.7354845392862692 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTA 1228 0.5300322853541893 No Hit GAATGATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTC 1120 0.4834170680754821 No Hit GAATGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCT 788 0.3401184371816785 No Hit GAATGATACCTGTCTCTTCTACACATCTGACGCAACGTTGGTCGTATGCCG 628 0.2710588560280382 No Hit CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT 585 0.2524990935929974 Illumina Single End Adapter 2 (95% over 21bp) GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGT 525 0.22660175066038227 No Hit CCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC 426 0.1838711348215673 No Hit GAATCTTTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC 413 0.17826004385283403 No Hit GAATGACTCTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCT 365 0.15754216950674194 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATG 349 0.1506362113913779 No Hit TCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC 348 0.15020458900916767 No Hit GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTAT 320 0.1381191623072806 No Hit GAATGCTACGGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGC 309 0.13337131610296785 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCC 306 0.13207644895633708 No Hit GAATGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTT 305 0.13164482657412682 No Hit GAATGATACGGCTGCCTCCTATACACATCTGACGCAACGTTGGTCGTATGC 297 0.1281918475164448 No Hit GAATCTGTCTCTTATCCACATCTGACGCAACGTTGGTCGTATGCCGTCTTC 286 0.12344400131213205 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 275 0.11869615510781927 No Hit GAATGCTACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCG 265 0.11437993128571675 No Hit GAATCTGTCTCTTATCCCCATCTGACGCAACGTTGGTCGTATGCCGTCTTC 259 0.11179019699245525 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 252 0.10876884031698349 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.3086100032803301 0.0 2 0.0 0.0 0.0 1.7411646898361561 0.0 3 0.0 0.0 0.0 2.0437319797655427 0.0 4 0.0 0.0 0.0 3.3575905112135493 0.0 5 0.0 0.0 0.0 6.244281003435714 0.0 6 0.0 0.0 0.0 6.71604426719152 0.0 7 0.0 0.0 0.0 7.382469225324148 0.0 8 0.0 0.0 0.0 8.246577234509072 0.0 9 0.0 0.0 0.0 8.470589250876193 0.0 10 0.0 0.0 0.0 13.086359006232627 0.0 11 0.0 0.0 0.0 13.916368847222941 0.0 12 0.0 0.0 0.0 18.1795031163136 0.0 13 0.0 0.0 0.0 18.62493741475458 0.0 14 0.0 0.0 0.0 18.891680046960516 0.0 15 0.0 0.0 0.0 19.747155608501235 0.0 16 0.0 0.0 0.0 20.226688075136824 0.0 17 0.0 0.0 0.0 20.593567100015537 0.0 18 0.0 0.0 0.0 20.95569827868994 0.0 19 0.0 0.0 0.0 22.381778629512613 0.0 20 0.0 0.0 0.0 22.75729010203553 0.0 21 0.0 0.0 0.0 23.13150670741182 0.0 22 0.0 0.0 0.0 23.76858134355415 0.0 23 0.0 0.0 0.0 24.122080074584346 0.0 24 0.0 0.0 0.0 24.40263462302101 0.0 25 0.0 0.0 0.0 24.634847464650125 0.0 26 0.0 0.0 0.0 24.901158474473853 0.0 27 0.0 0.0 0.0 25.149341344244746 0.0 28 0.0 0.0 0.0 25.34270817147494 0.0 29 0.0 0.0 0.0 25.54470744634934 0.0 30 0.0 0.0 0.0 25.7747621760674 0.0 31 0.0 0.0 0.0 25.983235786674953 0.0 32 0.0 0.0 0.0 26.190846152518084 0.0 33 0.0 0.0 0.0 26.398456518361215 0.0 34 0.0 0.0 0.0 26.61038310802645 0.0 35 0.0 0.0 0.0 26.811087515754217 0.0 36 0.0 0.0 0.0 27.00661245489546 0.0 37 0.0 0.0 0.0 27.216380932649642 0.0 38 0.0 0.0 0.0 27.41967507467067 0.0 39 0.0 0.0 0.0 27.61994786001623 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGACCG 30 2.1579744E-6 45.000004 11 TAGGGAC 30 2.1579744E-6 45.000004 5 GCGAGAC 20 7.021615E-4 45.0 21 ACCGGTG 55 1.8189894E-12 45.0 17 GGGCTTA 20 7.021615E-4 45.0 7 GGCGTTT 20 7.021615E-4 45.0 7 TATGGGC 25 3.881134E-5 45.0 4 TATGGGA 45 3.8198777E-10 45.0 4 GATCCGT 20 7.021615E-4 45.0 5 ATACTTC 20 7.021615E-4 45.0 42 ATCCGTC 20 7.021615E-4 45.0 6 TTATACG 25 3.881134E-5 45.0 26 GTATGGC 20 7.021615E-4 45.0 9 GCCCTCG 20 7.021615E-4 45.0 20 GCGATTG 25 3.881134E-5 45.0 9 ATGACTC 50 2.1827873E-11 45.0 3 TCCGTCT 20 7.021615E-4 45.0 7 TGCTACG 95 0.0 45.0 4 CTCCTAT 50 2.1827873E-11 45.0 16 CACCGGT 65 0.0 44.999996 16 >>END_MODULE