##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933364.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	617430
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.155199779732115	31.0	28.0	31.0	25.0	34.0
2	27.986753154203715	31.0	25.0	33.0	16.0	34.0
3	27.97461412629772	31.0	25.0	33.0	16.0	34.0
4	32.86498874366325	35.0	32.0	37.0	28.0	37.0
5	34.16902320910872	35.0	33.0	37.0	30.0	37.0
6	33.23918500882691	35.0	32.0	37.0	28.0	37.0
7	33.65711740602173	35.0	33.0	37.0	29.0	37.0
8	33.21066355700241	35.0	32.0	37.0	28.0	37.0
9	34.7134768313817	37.0	34.0	39.0	28.0	39.0
10	34.640087135383766	37.0	33.0	39.0	28.0	39.0
11	34.93512786874626	37.0	34.0	39.0	28.0	39.0
12	35.01182320263026	37.0	34.0	39.0	27.0	39.0
13	35.00468555139854	37.0	34.0	39.0	27.0	39.0
14	35.87443920768346	38.0	34.0	40.0	27.0	41.0
15	35.99346970506778	38.0	34.0	40.0	27.0	41.0
16	36.09776816805144	38.0	34.0	40.0	30.0	41.0
17	35.28876471826766	37.0	33.0	40.0	27.0	41.0
18	35.098388481285326	37.0	33.0	39.0	27.0	40.0
19	34.46319744748393	37.0	33.0	37.0	27.0	40.0
20	33.345467502388935	35.0	32.0	37.0	25.0	39.0
21	34.070683316327354	35.0	33.0	38.0	27.0	40.0
22	34.57600213789417	35.0	33.0	38.0	28.0	40.0
23	34.75446609332232	35.0	33.0	39.0	29.0	40.0
24	34.40943102861863	35.0	33.0	38.0	27.0	40.0
25	33.54106538393016	35.0	33.0	38.0	24.0	40.0
26	33.41936737767844	35.0	32.0	38.0	24.0	40.0
27	34.16097047438576	35.0	33.0	38.0	26.0	40.0
28	33.69632346986703	35.0	33.0	38.0	24.0	40.0
29	33.749548936721574	35.0	33.0	39.0	24.0	40.0
30	33.156513288955836	35.0	32.0	38.0	21.0	40.0
31	33.06180133780347	35.0	32.0	38.0	21.0	40.0
32	32.448815250311775	35.0	31.0	38.0	18.0	40.0
33	31.742489027096187	35.0	30.0	39.0	12.0	40.0
34	31.20203747793272	35.0	29.0	39.0	10.0	40.0
35	30.738052896684643	35.0	26.0	39.0	8.0	40.0
36	30.188670780493336	35.0	23.0	39.0	7.0	40.0
37	30.093354712275076	35.0	23.0	39.0	7.0	40.0
38	29.809615664933677	35.0	22.0	39.0	7.0	40.0
39	29.34610401179081	35.0	21.0	39.0	7.0	40.0
40	29.21364365191196	35.0	21.0	39.0	7.0	40.0
41	28.619398150397615	34.0	18.0	39.0	7.0	40.0
42	28.842903648996646	35.0	18.0	39.0	7.0	40.0
43	28.77571384610401	35.0	18.0	39.0	7.0	40.0
44	28.944189624734786	35.0	18.0	39.0	7.0	40.0
45	29.01354323567044	35.0	20.0	39.0	7.0	40.0
46	28.666232609364624	35.0	20.0	39.0	7.0	40.0
47	28.608151531347684	35.0	20.0	38.0	7.0	40.0
48	28.465419561731693	34.0	20.0	38.0	7.0	40.0
49	28.690444260887872	35.0	20.0	39.0	7.0	40.0
50	28.701328085774907	35.0	20.0	39.0	7.0	40.0
51	27.260321008049495	33.0	18.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	20.0
10	29.0
11	35.0
12	53.0
13	71.0
14	113.0
15	180.0
16	312.0
17	613.0
18	1318.0
19	2308.0
20	3855.0
21	5768.0
22	8396.0
23	12073.0
24	17002.0
25	24816.0
26	32083.0
27	34502.0
28	32006.0
29	29857.0
30	30599.0
31	34192.0
32	38941.0
33	45362.0
34	49757.0
35	56523.0
36	57194.0
37	53815.0
38	39739.0
39	5886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.89851481139562	2.1059877233046658	26.38825453897608	39.607242926323636
2	38.066015580713604	22.70038708841488	27.46222243817113	11.771374892700386
3	10.502405130946018	22.922598513191776	55.00574963963526	11.56924671622694
4	9.797709861846688	2.3698232998072655	74.26671849440423	13.565748343941825
5	25.51949208817194	2.8571659945256953	55.55139853910565	16.071943378196718
6	11.55094504640202	19.717700791992616	59.355554475811026	9.375799685794341
7	44.99603193884327	1.147174578494728	51.0373645595452	2.8194289231167904
8	42.65438997133278	15.649223393745041	37.704841034611206	3.9915456003109666
9	40.37413148049171	2.4469170594237406	36.27925432842589	20.899697131658648
10	30.0009717700792	13.99559464230763	42.64370050046159	13.359733087151579
11	25.41486484297815	13.202468296001166	48.313978912589285	13.068687948431402
12	18.697018285473657	11.341852517694313	50.2095784137473	19.751550783084724
13	17.436956416111947	12.896846606092998	58.641789352639165	11.024407625155888
14	14.754870997521985	20.807055050774988	47.696095103898415	16.74197884780461
15	10.418185057415416	12.957906159402684	61.56228236399268	15.061626419189219
16	12.47137327308359	15.835965210631164	47.297345448066984	24.39531606821826
17	13.462740715546701	16.709424550151432	54.36340961728455	15.464425117017313
18	13.10140420776444	15.421343310172814	52.20608004146219	19.271172440600555
19	12.988354955217595	18.494890109000213	48.49165087540288	20.025104060379313
20	15.429279432486274	17.29345836775019	55.8309443985553	11.446317801208234
21	15.227151256012828	23.829098035404822	48.52760636833325	12.416144340249097
22	12.419545535526295	13.054921205642744	53.4167435984646	21.108789660366355
23	13.782938956642859	22.430396968077353	50.8384756166691	12.948188458610693
24	16.038417310464347	16.127496234390943	47.45833535785433	20.375751097290383
25	12.375168035242861	26.822149879338546	45.31461056314076	15.48807152227783
26	12.604667735613754	14.00158722446269	53.03078891534263	20.362956124580926
27	16.33901818829665	17.771893170075963	47.30625334045965	18.582835301167744
28	10.60120175566461	18.304423173477154	53.954942260661134	17.139432810197107
29	17.288599517354193	13.985553018155905	51.3172343423546	17.408613122135304
30	12.849715757251834	21.266216413196638	52.83319566590544	13.050872163646082
31	17.384156908475454	17.893526391655733	45.531963137521664	19.19035356234715
32	22.79983155985294	19.88338759049609	43.163435531153326	14.153345318497642
33	14.828077676821664	16.30209092528708	41.227345610028664	27.64248578786259
34	18.523881249696323	18.69313120515686	47.86340151920056	14.919586025946261
35	14.725070048426542	19.19780379962101	42.61114620280841	23.465979949144035
36	15.582009296600424	23.868940608651993	39.85828353011677	20.69076656463081
37	19.72174983398928	19.99773253648187	44.453622272970215	15.826895356558637
38	17.4952626208639	24.407301232528383	33.86035663961906	24.237079506988646
39	22.65876293668918	19.922906240383526	40.61853165541033	16.799799167516966
40	16.87090034497838	19.23424517759098	38.343132014965256	25.55172246246538
41	16.74327454124354	23.04196427125342	37.719255624119334	22.495505563383702
42	16.77113195018059	20.556500332021443	41.21179728876148	21.46057042903649
43	18.852663459825404	19.35344897397276	35.28221822716745	26.511669339034384
44	20.206987026869445	19.770014414589507	38.727791004648296	21.295207553892748
45	15.992096270022513	20.686231637594545	36.97909074712923	26.342581345253713
46	24.26963380464182	22.907860000323925	33.48379573392935	19.338710461104903
47	14.151077854979514	19.514438883760103	46.29042968433669	20.0440535769237
48	19.068720340767374	23.26223215587192	34.9116499036328	22.757397599727906
49	16.700840581118506	18.809419691301038	42.06290591646017	22.42683381112029
50	18.53230325704938	18.250651895761465	38.14926388416501	25.06778096302415
51	18.138736374973682	18.925384254085483	36.58924898369046	26.346630387250375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	1019.5
2	1971.0
3	23918.0
4	45865.0
5	33606.0
6	21347.0
7	20344.0
8	19341.0
9	18702.0
10	18063.0
11	17111.0
12	16159.0
13	15129.5
14	14100.0
15	12916.5
16	11733.0
17	10678.5
18	9624.0
19	8919.0
20	8214.0
21	7616.5
22	7019.0
23	6664.0
24	6309.0
25	6150.5
26	6210.0
27	6428.0
28	6888.0
29	7348.0
30	7665.0
31	7982.0
32	8521.0
33	9060.0
34	9640.5
35	10221.0
36	10824.5
37	11428.0
38	12714.5
39	14001.0
40	14330.0
41	14659.0
42	16101.0
43	17543.0
44	20671.0
45	23799.0
46	70741.0
47	117683.0
48	78725.0
49	39767.0
50	35250.5
51	30734.0
52	25933.0
53	21132.0
54	20338.0
55	19544.0
56	18763.5
57	17983.0
58	16928.0
59	15873.0
60	14380.5
61	12888.0
62	11452.0
63	10016.0
64	8648.5
65	7281.0
66	6424.0
67	5567.0
68	4654.5
69	3742.0
70	3087.5
71	2433.0
72	2083.5
73	1734.0
74	1510.5
75	963.0
76	639.0
77	495.5
78	352.0
79	328.5
80	305.0
81	219.0
82	133.0
83	88.0
84	43.0
85	26.5
86	10.0
87	7.0
88	4.0
89	4.5
90	5.0
91	2.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	617430.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.108335671035604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.1902193126026	35.45110015033174
2	5.424357947832805	4.5682137053438705
3	2.189338805636121	2.765682399760499
4	1.4625125884028125	2.4633588398232305
5	1.069392026643517	2.2515159210917135
6	0.8632206672594473	2.1809271369081684
7	0.753125300983199	2.2198997083305185
8	0.6578908128415374	2.216214974561753
9	0.572434428823246	2.1693834970692105
>10	2.6888217895435473	17.348940429651996
>50	0.07526199894880058	2.2210375411979983
>100	0.0452351910653737	3.5277813488808296
>500	0.0027297098056691025	0.6890044004796109
>1k	0.0035096268930031316	4.363749271388384
>5k	3.899585436670147E-4	0.853702068182435
>10k+	0.0015598341746680588	14.709488606997983
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCG	25297	4.097144615583953	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22221	3.5989504883144647	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGC	21936	3.5527914095525	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTC	20126	3.2596407689940556	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGCTCCT	5199	0.84203877362616	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTC	4811	0.7791976418379412	No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCT	4575	0.7409746853894369	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTA	4295	0.6956254150268046	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCT	4066	0.6585361903373662	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	3827	0.6198273488492623	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGT	1612	0.26108222794486824	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATG	1139	0.18447435336799312	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTAT	1128	0.18269277488946115	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTT	1122	0.1817210048102619	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	678	0.10981001894951656	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	666	0.10786647879111803	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09345188928299564	0.0
2	0.0	0.0	0.0	0.8546717846557504	0.0
3	0.0	0.0	0.0	0.9699885007207295	0.0
4	0.0	0.0	0.0	1.92118944657694	0.0
5	0.0	0.0	0.0	5.919699399122168	0.0
6	0.0	0.0	0.0	6.224673242310869	0.0
7	0.0	0.0	0.0	7.07464813825049	0.0
8	0.0	0.0	0.0	8.072817971267998	0.0
9	0.0	0.0	0.0	8.216316019629756	0.0
10	0.0	0.0	0.0	13.136387930615616	0.0
11	1.6196167986654359E-4	0.0	0.0	13.389534036247024	0.0
12	1.6196167986654359E-4	0.0	0.0	17.70484103461121	0.0
13	1.6196167986654359E-4	0.0	0.0	18.06196653871694	0.0
14	1.6196167986654359E-4	0.0	0.0	18.33746335616993	0.0
15	1.6196167986654359E-4	0.0	0.0	19.245744456861505	0.0
16	1.6196167986654359E-4	0.0	0.0	19.662471859158124	0.0
17	1.6196167986654359E-4	0.0	0.0	19.854396449799978	0.0
18	1.6196167986654359E-4	0.0	0.0	20.003887080316797	0.0
19	1.6196167986654359E-4	0.0	0.0	21.152519313930323	0.0
20	1.6196167986654359E-4	0.0	0.0	21.38185705262135	0.0
21	1.6196167986654359E-4	0.0	0.0	21.541389307289894	0.0
22	1.6196167986654359E-4	0.0	0.0	21.892036344200964	0.0
23	1.6196167986654359E-4	0.0	0.0	22.043794438235913	0.0
24	1.6196167986654359E-4	0.0	0.0	22.180652057723144	0.0
25	1.6196167986654359E-4	0.0	0.0	22.285603226276663	0.0
26	1.6196167986654359E-4	0.0	0.0	22.425214194321622	0.0
27	1.6196167986654359E-4	0.0	0.0	22.65082681437572	0.0
28	1.6196167986654359E-4	0.0	0.0	22.765981568760832	0.0
29	1.6196167986654359E-4	0.0	0.0	22.89911406961113	0.0
30	1.6196167986654359E-4	0.0	0.0	23.04066857781449	0.0
31	1.6196167986654359E-4	0.0	0.0	23.160196297556	0.0
32	1.6196167986654359E-4	0.0	0.0	23.303046499198288	0.0
33	1.6196167986654359E-4	0.0	0.0	23.435693115008988	0.0
34	1.6196167986654359E-4	0.0	0.0	23.673290899373207	0.0
35	1.6196167986654359E-4	0.0	0.0	23.83622434931895	0.0
36	1.6196167986654359E-4	0.0	0.0	23.972596083766582	0.0
37	1.6196167986654359E-4	0.0	0.0	24.151077854979512	0.0
38	1.6196167986654359E-4	0.0	0.0	24.34883306609656	0.0
39	1.6196167986654359E-4	0.0	0.0	24.644575093532872	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAATAC	20	7.030746E-4	45.000004	16
TAGGGTA	20	7.030746E-4	45.000004	5
GCGATAT	20	7.030746E-4	45.000004	9
CGCACAT	20	7.030746E-4	45.000004	31
TAGTATA	20	7.030746E-4	45.000004	12
ACGGGAC	25	3.8887018E-5	45.0	5
GATACGC	30	2.1638643E-6	44.999996	5
CGTTTTT	16980	0.0	44.469963	1
GATACCT	3055	0.0	43.01146	5
TGATACC	3060	0.0	42.64706	4
CCGATGA	560	0.0	42.589287	18
TACGGCT	2625	0.0	42.514286	7
CGGCTGT	2680	0.0	42.229477	9
ACCGGTG	80	0.0	42.187504	17
ATACCTG	3120	0.0	42.1875	6
ACGGCTG	2660	0.0	42.039474	8
ACCGGTC	70	0.0	41.785717	17
TACGAAA	70	0.0	41.785717	20
ACATACG	70	0.0	41.785717	17
CGATGAA	585	0.0	41.153843	19
>>END_MODULE