SRR2933363.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933363.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415734
Sequences flagged as poor quality	0
Sequence length	51
%GC	35

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

$Per base sequence content$

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32799	7.889419677005008	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCG	11526	2.7724458427744665	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGC	8471	2.0376009660022993	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTC	5853	1.4078713792954147	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGCTCCT	2376	0.5715192887759962	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	1891	0.45485815449301714	TruSeq Adapter, Index 20 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCT	1617	0.38895062708366407	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTC	1598	0.3843803970808257	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTA	1448	0.34829963390052293	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCT	977	0.23500603751437218	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTTAGCTCCTCGTATGCCG	888	0.2135981180273925	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGT	585	0.1407149764031809	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCT	504	0.12123136428581738	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	487	0.11714221112538306	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCC	457	0.10992605848932249	No Hit
GAATCTTTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTC	445	0.10703959743489828	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCTTAGCTCCTCGTATGC	444	0.10679905901369625	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	434	0.10439367480167608	No Hit
GAATGACTCTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCT	424	0.10198829058965589	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATACG	30	2.1621454E-6	45.000004	17
CATGGGT	40	6.7975634E-9	45.0	4
CCGATGA	140	0.0	45.0	18
ATCCGGC	40	6.7975634E-9	45.0	6
CAAGGTC	20	7.0280867E-4	45.0	17
TGATTCG	20	7.0280867E-4	45.0	26
GGACCTA	20	7.0280867E-4	45.0	8
ATCGGGA	20	7.0280867E-4	45.0	4
CGATCGT	25	3.8864957E-5	45.0	10
CGTTTTT	13825	0.0	44.62568	1
TACGGCT	1220	0.0	43.340164	7
TGATACC	1525	0.0	43.229507	4
CGATGAA	125	0.0	43.199997	19
ACGGCTG	1225	0.0	42.97959	8
CTACGGC	100	0.0	42.75	6
GATACCT	1555	0.0	42.540195	5
ATGATAC	4130	0.0	41.840195	3
GAATGAT	4410	0.0	41.683674	1
AATTATC	65	0.0	41.53846	45
AATGATA	4125	0.0	41.399998	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content