##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933363.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415734
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.19513679420014	31.0	26.0	31.0	16.0	33.0
2	28.20310583209456	31.0	28.0	33.0	16.0	34.0
3	28.358803946754414	31.0	28.0	33.0	16.0	34.0
4	31.66593061909779	35.0	28.0	37.0	19.0	37.0
5	33.58527327570033	35.0	33.0	37.0	28.0	37.0
6	32.17024106760573	35.0	32.0	37.0	17.0	37.0
7	32.293709439208726	35.0	32.0	35.0	26.0	37.0
8	32.517232172494914	35.0	32.0	36.0	25.0	37.0
9	33.105608393828746	35.0	32.0	37.0	25.0	39.0
10	31.872339043715453	35.0	28.0	39.0	15.0	39.0
11	32.56658344037293	35.0	30.0	39.0	17.0	39.0
12	33.37010925255091	35.0	31.0	39.0	23.0	39.0
13	33.47354798982041	35.0	32.0	39.0	25.0	39.0
14	33.77424507016506	36.0	32.0	40.0	21.0	41.0
15	34.18882025525937	37.0	32.0	40.0	25.0	41.0
16	34.419924759581846	37.0	32.0	40.0	25.0	41.0
17	32.44080108915797	35.0	27.0	40.0	18.0	41.0
18	33.04987083086782	36.0	30.0	39.0	23.0	39.0
19	32.8016688555663	36.0	30.0	37.0	21.0	39.0
20	32.08837622133383	34.0	30.0	36.0	23.0	39.0
21	32.58222324851948	35.0	31.0	37.0	24.0	39.0
22	32.66231292124291	34.0	30.0	37.0	24.0	39.0
23	33.589218105808044	35.0	32.0	38.0	26.0	39.0
24	32.10577436533938	34.0	31.0	37.0	21.0	39.0
25	31.45254898564948	34.0	30.0	37.0	18.0	39.0
26	30.3152761140537	33.0	25.0	37.0	18.0	39.0
27	32.190927371829105	34.0	30.0	37.0	22.0	39.0
28	32.05247105120101	35.0	30.0	37.0	21.0	39.0
29	32.198660682070745	35.0	30.0	38.0	21.0	40.0
30	31.38212414668995	34.0	29.0	37.0	18.0	40.0
31	31.64377703050508	34.0	30.0	37.0	20.0	39.0
32	31.45652749113616	35.0	30.0	37.0	18.0	39.0
33	31.193154757609435	35.0	30.0	38.0	15.0	40.0
34	30.299734926659834	35.0	27.0	38.0	10.0	40.0
35	29.758675018160652	35.0	24.0	38.0	8.0	40.0
36	28.961797687944696	34.0	22.0	37.0	7.0	40.0
37	29.06345163012888	34.0	22.0	38.0	7.0	40.0
38	28.887690205756567	34.0	22.0	38.0	7.0	40.0
39	28.4586370130901	34.0	21.0	37.0	7.0	40.0
40	28.269075899493426	33.0	21.0	37.0	7.0	40.0
41	27.64346192517331	33.0	18.0	37.0	7.0	40.0
42	28.138268700659555	33.0	20.0	37.0	7.0	40.0
43	28.112100525816988	33.0	18.0	37.0	7.0	40.0
44	28.038765171960918	33.0	18.0	37.0	7.0	40.0
45	28.23911202836429	34.0	20.0	38.0	7.0	40.0
46	27.8161973762069	33.0	18.0	37.0	7.0	40.0
47	27.898562542394895	33.0	18.0	37.0	7.0	40.0
48	27.715919313791993	33.0	18.0	37.0	7.0	40.0
49	27.710062203235722	33.0	18.0	37.0	7.0	39.0
50	27.89385039472355	33.0	19.0	37.0	7.0	40.0
51	26.1277716039583	31.0	16.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	9.0
10	22.0
11	33.0
12	35.0
13	68.0
14	86.0
15	202.0
16	432.0
17	866.0
18	1546.0
19	2408.0
20	3888.0
21	5479.0
22	7674.0
23	10383.0
24	14085.0
25	19183.0
26	24135.0
27	26038.0
28	25448.0
29	25679.0
30	27426.0
31	30869.0
32	33628.0
33	36098.0
34	38311.0
35	39394.0
36	26121.0
37	13308.0
38	2818.0
39	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.09056271558256	2.1872158639899553	28.990652676952088	43.7315687434754
2	43.401550029586225	15.5625953133494	28.997868829588153	12.037985827476223
3	11.133080286914229	15.486825710670765	61.49942030240491	11.880673700010103
4	10.321984730621022	2.443629820991307	73.14412581121582	14.090259637171846
5	20.89004026613171	2.948519967094344	60.942333318901035	15.219106447872917
6	11.944897458471042	13.829756527010058	63.34555268513039	10.879793329388503
7	45.06439213535578	1.1947543381104264	50.74422587519904	2.9966276513347476
8	45.011233144270136	10.94137116521622	39.92745361216547	4.119942078348174
9	41.72932692538979	2.382773600427196	39.74440387363074	16.143495600552278
10	27.3242024948645	14.599238936435317	45.15723996593976	12.919318602760418
11	22.530512298729477	13.804499992783848	51.589958964145346	12.075028744341333
12	18.194326179720687	11.91964092424483	52.80612122174275	17.079911674291733
13	16.22503812533976	13.17693525186778	59.421889958483064	11.17613666430939
14	13.794156840672162	17.376495547633823	51.99959589545238	16.82975171624164
15	10.35878710906493	13.20772416978164	61.67573496514598	14.757753756007446
16	12.379550385583089	13.668595784804708	51.65105572313065	22.300798106481547
17	13.298647692996003	14.958122260868729	56.13517297117868	15.608057074956584
18	12.92412937118446	13.656087786902201	55.26947519327262	18.15030764864072
19	13.207002554518033	17.01135822424916	51.71143086685236	18.070208354380448
20	14.979530180355708	16.032366849956944	57.136053341800284	11.852049627887062
21	15.247249443153555	20.4481711863836	51.225062179181876	13.079517191280962
22	12.642218341535694	13.457643589410537	54.962548167818845	18.937589901234926
23	13.393179292528396	20.23553522204102	52.37555744779113	13.995728037639452
24	14.419556735797409	15.104610159380757	51.43433060562763	19.041502499194195
25	12.587616119922835	21.442075942790343	49.3322653427432	16.638042594543627
26	12.259521713403283	14.760880755483075	55.12563321739381	17.853964313719832
27	13.748935617486179	16.710204120904233	51.820635310078075	17.720224951531506
28	10.916595707832412	17.149186739597916	55.90136962577033	16.03284792679935
29	16.10597160684476	13.723438544838768	53.58955485959772	16.58103498871875
30	12.943612983301822	18.92532244175362	54.52476824123117	13.606296333713383
31	15.761520587683469	16.678212510884364	49.85495533201518	17.70531156941698
32	19.33135129674263	18.62392779998749	47.50248957265944	14.54223133061044
33	14.363270745236138	15.805298580342239	46.032799819115105	23.79863085530652
34	16.62769944243194	18.15776433969798	49.73179966035975	15.482736557510332
35	13.617842177931081	18.98353273968451	46.342853844044505	21.055771238339897
36	15.04279178513184	21.886831483592875	43.80276811615119	19.267608615124093
37	18.477680439896666	19.906719200257857	45.84830685005316	15.767293509792319
38	17.01785276162161	22.469415539744165	39.12429582377193	21.388435874862292
39	19.836963058109273	19.10404248870672	44.0416227684048	17.01737168477921
40	16.442484858106386	18.3386492324419	41.902755127076446	23.31611078237527
41	15.942646018848592	20.831348891358417	41.54844203264588	21.67756305714712
42	15.98810778045577	19.518249650016596	43.67720706028374	20.81643550924389
43	17.251415568608774	18.659046409482986	40.09150081542525	23.99803720648299
44	19.075658955004883	19.33399721937585	41.93763319815074	19.652710627468526
45	15.728085747136388	20.1260902403941	40.54130766307302	23.60451634939649
46	20.739703752880448	21.76704334983427	38.245368432699756	19.24788446458553
47	14.18623446723145	19.004219043907884	46.949972819158404	19.859573669702264
48	17.946331067461404	21.44664617279318	39.183708813808835	21.423313945936584
49	16.157687367403195	18.286933471883465	42.96858087142259	22.586798289290748
50	17.27739371809859	17.630263582001955	40.776313700587394	24.31602899931206
51	17.0637956000712	18.010314287501142	39.933948149537926	24.991941962889733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	92.0
1	394.0
2	696.0
3	23856.5
4	47017.0
5	31177.0
6	15337.0
7	14605.0
8	13873.0
9	13567.0
10	13261.0
11	12425.0
12	11589.0
13	10510.5
14	9432.0
15	8620.5
16	7809.0
17	7010.0
18	6211.0
19	5729.5
20	5248.0
21	4780.0
22	4312.0
23	4109.5
24	3907.0
25	3756.0
26	3777.5
27	3950.0
28	4132.0
29	4314.0
30	4583.0
31	4852.0
32	5282.5
33	5713.0
34	6117.5
35	6522.0
36	7047.0
37	7572.0
38	8262.5
39	8953.0
40	9436.5
41	9920.0
42	11304.0
43	12688.0
44	14124.5
45	15561.0
46	33810.5
47	52060.0
48	41466.0
49	30872.0
50	27854.5
51	24837.0
52	20708.5
53	16580.0
54	15199.0
55	13818.0
56	12879.0
57	11940.0
58	11108.0
59	10276.0
60	9421.0
61	8566.0
62	7641.0
63	6716.0
64	5864.5
65	5013.0
66	4512.0
67	4011.0
68	3460.0
69	2909.0
70	2437.0
71	1965.0
72	1673.5
73	1382.0
74	1196.5
75	783.0
76	555.0
77	451.0
78	347.0
79	303.5
80	260.0
81	186.0
82	112.0
83	74.0
84	36.0
85	27.0
86	18.0
87	13.0
88	8.0
89	5.5
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	415734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.465867344580786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.12208433826608	36.516297082458145
2	6.5611395465005575	5.834935214079535
3	2.8417750554460635	3.7908597791581005
4	1.8447326099996677	3.2811054208987
5	1.3507554195252724	3.0031255649792166
6	1.0248631874365637	2.7342858323339083
7	0.8109672253140212	2.5242252743131215
8	0.6530568209638601	2.3230990375562115
9	0.5083211243874672	2.0342645716915135
>10	2.1605239734615593	14.371556805510439
>50	0.07163570511702774	2.204992961850961
>100	0.042981423070216636	4.098802340909309
>500	0.0022041755420623916	0.7238081130467539
>1k	0.0027552194275779892	2.188086137274039
>5k	0.0011020877710311958	3.5097587715916387
>10k+	0.0011020877710311958	10.860797092348463
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32799	7.889419677005008	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCG	11526	2.7724458427744665	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGC	8471	2.0376009660022993	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTC	5853	1.4078713792954147	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGCTCCT	2376	0.5715192887759962	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	1891	0.45485815449301714	TruSeq Adapter, Index 20 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCT	1617	0.38895062708366407	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTC	1598	0.3843803970808257	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTA	1448	0.34829963390052293	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCT	977	0.23500603751437218	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTTAGCTCCTCGTATGCCG	888	0.2135981180273925	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGT	585	0.1407149764031809	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCT	504	0.12123136428581738	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	487	0.11714221112538306	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCC	457	0.10992605848932249	No Hit
GAATCTTTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTC	445	0.10703959743489828	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCTTAGCTCCTCGTATGC	444	0.10679905901369625	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	434	0.10439367480167608	No Hit
GAATGACTCTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCT	424	0.10198829058965589	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1589958964145343	0.0
2	0.0	0.0	0.0	0.9289593826821958	0.0
3	0.0	0.0	0.0	1.1324548870191036	0.0
4	0.0	0.0	0.0	1.8723510706365127	0.0
5	0.0	0.0	0.0	3.9104331134812162	0.0
6	0.0	0.0	0.0	4.285191973713961	0.0
7	0.0	0.0	0.0	4.807641424564745	0.0
8	0.0	0.0	0.0	5.546334916076145	0.0
9	0.0	0.0	0.0	5.77220049358484	0.0
10	0.0	0.0	0.0	9.557072551198603	0.0
11	0.0	0.0	0.0	10.296006581131204	0.0
12	0.0	0.0	0.0	13.22432132084458	0.0
13	0.0	0.0	0.0	13.533413192089172	0.0
14	0.0	0.0	0.0	13.74532754116815	0.0
15	0.0	0.0	0.0	14.368322052081378	0.0
16	0.0	0.0	0.0	14.737548528626478	0.0
17	0.0	0.0	0.0	15.053375475664728	0.0
18	0.0	0.0	0.0	15.350680964270422	0.0
19	0.0	0.0	0.0	16.293351036961134	0.0
20	0.0	0.0	0.0	16.60388613873294	0.0
21	0.0	0.0	0.0	16.92476439261643	0.0
22	0.0	0.0	0.0	17.40127100501763	0.0
23	0.0	0.0	0.0	17.704108877310972	0.0
24	0.0	0.0	0.0	17.945849990619003	0.0
25	0.0	0.0	0.0	18.144775264953072	0.0
26	0.0	0.0	0.0	18.34009246296911	0.0
27	0.0	0.0	0.0	18.579187653643917	0.0
28	0.0	0.0	0.0	18.776910235871977	0.0
29	0.0	0.0	0.0	19.005421736013894	0.0
30	0.0	0.0	0.0	19.235135928261823	0.0
31	0.0	0.0	0.0	19.440315201547143	0.0
32	0.0	0.0	0.0	19.64332962904165	0.0
33	0.0	0.0	0.0	19.830227982315616	0.0
34	0.0	0.0	0.0	20.048636868767048	0.0
35	0.0	0.0	0.0	20.27979429154219	0.0
36	0.0	0.0	0.0	20.46957910587058	0.0
37	0.0	0.0	0.0	20.664174688623014	0.0
38	0.0	0.0	0.0	20.900383418243397	0.0
39	0.0	0.0	0.0	21.27610443216095	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATACG	30	2.1621454E-6	45.000004	17
CATGGGT	40	6.7975634E-9	45.0	4
CCGATGA	140	0.0	45.0	18
ATCCGGC	40	6.7975634E-9	45.0	6
CAAGGTC	20	7.0280867E-4	45.0	17
TGATTCG	20	7.0280867E-4	45.0	26
GGACCTA	20	7.0280867E-4	45.0	8
ATCGGGA	20	7.0280867E-4	45.0	4
CGATCGT	25	3.8864957E-5	45.0	10
CGTTTTT	13825	0.0	44.62568	1
TACGGCT	1220	0.0	43.340164	7
TGATACC	1525	0.0	43.229507	4
CGATGAA	125	0.0	43.199997	19
ACGGCTG	1225	0.0	42.97959	8
CTACGGC	100	0.0	42.75	6
GATACCT	1555	0.0	42.540195	5
ATGATAC	4130	0.0	41.840195	3
GAATGAT	4410	0.0	41.683674	1
AATTATC	65	0.0	41.53846	45
AATGATA	4125	0.0	41.399998	2
>>END_MODULE