FastQCFastQC Report
Sat 14 Jan 2017
SRR2933361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501349
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407218.122286072177266No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG104522.0847752763045304No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC77931.5544062120399165No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC69191.3800765534587682No Hit
GAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT27140.5413394661204072TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGAACACT21340.42565159200477115No Hit
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC19430.38755437828738065TruSeq Adapter, Index 19 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTC16370.32651905159878647No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTA11370.22678812563703127No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCT9990.19926239007158683No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT8810.17572589154461263No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCTGAACACTCGTATGCCG8450.16854526487536625No Hit
GAATCTTTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC7180.14321360968108043No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6320.12605989041565854No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCC5680.11329433189255389No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGAACACTCGT5640.11249648448485984No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA5270.10511639596368996No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5210.1039196248521489No Hit
GAATCTGTCTCTTATCCACATCTGACGCCTGAACACTCGTATGCCGTCTTC5190.10352070114830188No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATT207.029478E-445.022
CGCATTT253.8876486E-545.023
TACGAAA453.8380676E-1045.020
TAGGGCG253.8876486E-545.05
TTCGGTC253.8876486E-545.010
TATACGG406.8012014E-945.027
CCACCGT253.8876486E-545.015
CGTTTTT187750.044.640481
CGACCAC8000.042.4687512
TACGGCT9000.042.07
GATACCT13250.041.7735865
GAATGAT39750.041.037741
TGATACC13600.041.0294154
GCGACCA8350.040.95808411
ACGGCTG9250.040.8648648
ATGATAC37450.040.794393
GATACGG24050.040.6029135
AATGATA37350.040.602412
ACCACCG4550.040.5494514
GAATCTG11550.040.5194781
Produced by FastQC (version 0.11.5)