##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933361.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	501349
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.40651322731271	31.0	27.0	31.0	16.0	33.0
2	28.55954036010843	31.0	28.0	33.0	16.0	34.0
3	28.665781720916964	31.0	28.0	33.0	16.0	34.0
4	31.930511480026887	35.0	30.0	37.0	19.0	37.0
5	33.606621335636454	35.0	33.0	37.0	28.0	37.0
6	32.63181735677143	35.0	33.0	37.0	17.0	37.0
7	32.41909727555056	35.0	32.0	37.0	26.0	37.0
8	32.89267755595404	35.0	32.0	37.0	27.0	37.0
9	33.243018336528046	35.0	32.0	38.0	25.0	39.0
10	32.35528942912023	35.0	28.0	39.0	16.0	39.0
11	33.07987250398425	37.0	31.0	39.0	17.0	39.0
12	33.78225946396622	37.0	31.0	39.0	25.0	39.0
13	33.863976990080765	37.0	32.0	39.0	25.0	39.0
14	34.21962744515298	37.0	32.0	40.0	23.0	41.0
15	34.608807437533535	37.0	32.0	40.0	25.0	41.0
16	34.74246482988896	37.0	32.0	40.0	25.0	41.0
17	32.839734396597976	36.0	29.0	40.0	18.0	41.0
18	33.33263654659728	36.0	31.0	39.0	23.0	39.0
19	33.00273861122691	36.0	31.0	37.0	23.0	39.0
20	32.19575186147773	34.0	30.0	35.0	24.0	39.0
21	32.57497870744731	35.0	31.0	36.0	24.0	39.0
22	32.477017008112114	34.0	30.0	37.0	24.0	39.0
23	33.52920221243086	35.0	32.0	38.0	26.0	39.0
24	31.842279529828524	34.0	30.0	36.0	21.0	39.0
25	31.191052540246414	34.0	29.0	36.0	18.0	39.0
26	29.91462833275822	33.0	25.0	36.0	18.0	39.0
27	31.922900015757488	34.0	30.0	37.0	22.0	39.0
28	31.9378855846925	35.0	30.0	37.0	21.0	39.0
29	31.916186129821742	35.0	30.0	37.0	21.0	39.0
30	31.048858180628663	34.0	27.0	37.0	18.0	39.0
31	31.343804415686478	34.0	30.0	36.0	18.0	39.0
32	31.0333799409194	35.0	30.0	37.0	16.0	39.0
33	30.468254648957114	35.0	27.0	37.0	12.0	40.0
34	29.263572880368766	34.0	23.0	37.0	8.0	39.0
35	28.560794975157027	34.0	21.0	37.0	7.0	39.0
36	27.914761972199006	33.0	18.0	37.0	7.0	39.0
37	27.81954486794628	34.0	18.0	37.0	7.0	40.0
38	27.791133521758297	33.0	18.0	37.0	7.0	40.0
39	27.441026111551036	33.0	18.0	37.0	7.0	39.0
40	27.09382087128926	33.0	16.0	37.0	7.0	39.0
41	26.819118019583165	33.0	15.0	37.0	7.0	39.0
42	27.046735906524198	33.0	15.0	37.0	7.0	40.0
43	27.244665891424937	33.0	15.0	37.0	7.0	40.0
44	27.090936652910447	33.0	15.0	37.0	7.0	40.0
45	27.312516829593758	33.0	15.0	37.0	7.0	40.0
46	27.06600192680149	33.0	15.0	37.0	7.0	40.0
47	27.100510821802775	33.0	15.0	37.0	7.0	40.0
48	26.895928784140388	33.0	15.0	37.0	7.0	39.0
49	26.675437669168584	33.0	15.0	37.0	7.0	39.0
50	26.87905431146766	33.0	15.0	37.0	7.0	40.0
51	25.506684963967217	31.0	12.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	18.0
10	30.0
11	26.0
12	51.0
13	82.0
14	139.0
15	263.0
16	590.0
17	1194.0
18	2139.0
19	3622.0
20	5411.0
21	7598.0
22	10856.0
23	14587.0
24	19536.0
25	26506.0
26	31928.0
27	32383.0
28	29917.0
29	29300.0
30	31360.0
31	34331.0
32	38522.0
33	42247.0
34	44737.0
35	46162.0
36	30028.0
37	14757.0
38	2979.0
39	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.190928873898223	2.0859720474160715	28.30822441054036	47.414874668145345
2	47.625107460072726	13.087091028405363	27.278602330911202	12.009199180610713
3	10.243163943679951	13.068541076176476	64.30570321273204	12.382591767411524
4	9.76246088054429	2.462556023847659	73.6147873038542	14.16019579175385
5	17.564610680384323	2.845722241392722	64.8787571133083	14.71090996491466
6	11.67330542197152	10.798266277583082	67.21325862822106	10.31516967222434
7	43.18129686106884	1.1646577533813771	52.826474172682104	2.8275712128676833
8	43.28641325703252	8.206658435540911	44.58730345527766	3.9196248521489023
9	39.787652912442226	2.4573700156976477	44.219296338478784	13.535680733381335
10	23.82591767411524	14.035931058005502	49.88560862792187	12.252542639957396
11	19.203588717639807	13.496187286700481	55.00160566790798	12.298618327751726
12	15.55922122114535	11.703025237908124	57.483310029540306	15.254443511406226
13	14.700139024910792	12.66024266528905	62.266205776814154	10.373412532986004
14	12.71589252197571	15.799971676417027	56.92800823378524	14.556127567822017
15	10.203072111443326	13.344795741090538	62.84205214331733	13.610080004148806
16	11.548043378963555	13.796576835697289	55.18650680464108	19.468872980698077
17	12.410117502976968	14.97459853315754	58.24645107499965	14.36883288886584
18	12.478133994482885	13.808544546812698	57.36782161727659	16.345499841427827
19	12.669816834181379	16.494697306666612	54.1532944116773	16.682191447474715
20	13.616263321558437	16.318173567714307	58.64577370255052	11.41978940817674
21	14.239382146967483	18.45301376885164	55.13684080351212	12.170763280668757
22	12.25792810995933	13.41041869037337	57.4204795461844	16.9111736534829
23	12.627929845277441	18.295438905832064	55.88043458748297	13.196196661407523
24	13.677498110098954	15.071337531340442	54.63120500888603	16.61995934967458
25	12.114116114722478	19.79240010451801	52.89548797344764	15.197995807311873
26	12.33970746924797	14.265312187717537	56.39664186026102	16.998338482773477
27	13.817121406445409	15.305505745498646	54.360934199529666	16.516438648526275
28	11.489002670794198	15.511150914831784	56.658136348132736	16.34171006624128
29	14.5984134804298	14.03014666429972	53.87384835713246	17.497591498138025
30	13.150120973613191	17.384696089949315	56.08069428681417	13.384488649623316
31	16.09677091207921	15.377511474043033	51.58023652186401	16.945481092013747
32	15.638407576358984	20.88385535824346	49.77690191862356	13.700835146774004
33	13.699239451958617	18.58545643852885	47.95601467241383	19.759289437098708
34	15.531097100024136	19.758092665987167	45.17990461734241	19.530905616646287
35	12.80006542348743	23.012512241971162	45.62430562342799	18.563116711113416
36	17.46168836479179	20.07264400647054	41.03409002511225	21.431577603625417
37	14.712904583433895	24.17617268609292	44.420752808921534	16.690169921551654
38	18.827204203060145	24.109951351254317	39.83632160431157	17.226522841373974
39	17.261628127312513	22.650090057026144	39.922888048046374	20.165393767614976
40	16.652072707834265	25.214770549058642	41.64524113940588	16.487915603701214
41	16.662843647838134	22.106955434238422	39.47030910603193	21.759891811891517
42	16.414713104045287	23.532509289935753	42.925985690606744	17.126791915412216
43	17.73315594525969	20.463788698092547	39.889976842478994	21.913078514168774
44	18.6622492515194	20.797288914508655	40.34853964005114	20.191922193920803
45	16.940893469419507	20.510861695146495	40.19355778110658	22.354687054327425
46	19.009113412014386	23.13398450979258	38.64393865351282	19.21296342468021
47	14.899401414982377	20.569304017760086	45.61393360712797	18.91736096012957
48	17.27878184657793	21.685891464827893	39.04206450995215	21.993262178642023
49	15.649377978214776	20.972815344201344	42.7007932597851	20.67701341779878
50	16.586250296699504	19.35438187769398	41.42942341562465	22.62994440998187
51	16.690369383403578	19.821322073046918	39.76212179539602	23.726186748153484
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	463.5
2	841.0
3	29347.0
4	57853.0
5	38935.0
6	20017.0
7	18782.5
8	17548.0
9	17156.0
10	16764.0
11	16098.5
12	15433.0
13	14438.0
14	13443.0
15	12729.5
16	12016.0
17	11118.5
18	10221.0
19	9466.0
20	8711.0
21	8093.0
22	7475.0
23	6954.5
24	6434.0
25	6090.5
26	5678.0
27	5609.0
28	5777.5
29	5946.0
30	6307.0
31	6668.0
32	6992.0
33	7316.0
34	7854.5
35	8393.0
36	8819.5
37	9246.0
38	10024.0
39	10802.0
40	11360.5
41	11919.0
42	12985.5
43	14052.0
44	15633.0
45	17214.0
46	35897.0
47	54580.0
48	43794.0
49	33008.0
50	30132.5
51	27257.0
52	23182.5
53	19108.0
54	17269.5
55	15431.0
56	14593.5
57	13756.0
58	12680.0
59	11604.0
60	10533.5
61	9463.0
62	8531.0
63	7599.0
64	6791.5
65	5984.0
66	5149.0
67	4314.0
68	3757.5
69	3201.0
70	2725.5
71	2250.0
72	1883.5
73	1517.0
74	1325.0
75	914.5
76	696.0
77	514.5
78	333.0
79	253.0
80	173.0
81	137.5
82	102.0
83	73.0
84	44.0
85	34.5
86	25.0
87	17.5
88	10.0
89	7.5
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	501349.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.419314678704644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.46151519144797	43.74995428381999
2	7.348758097793477	7.704337264518303
3	3.1459572974600767	4.9472677662398095
4	1.8662442970108242	3.9130898828939333
5	1.2790308796535084	3.3522961082168825
6	0.8574324867033323	2.696761400174773
7	0.5418884878342437	1.988379621518544
8	0.38874217580907616	1.6302078754096228
9	0.2716343419897691	1.2814997445274123
>10	0.7436514931844898	6.157862879781531
>50	0.04757262556145798	1.7235082417323473
>100	0.040224008596613625	4.1845912367717
>500	0.0038676931342897713	1.3733731123009447
>1k	0.0019338465671448857	1.9392255416531643
>5k	7.735386268579544E-4	2.982737707140758
>10k+	7.735386268579544E-4	10.374907333300301
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40721	8.122286072177266	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG	10452	2.0847752763045304	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	7793	1.5544062120399165	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC	6919	1.3800765534587682	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT	2714	0.5413394661204072	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGAACACT	2134	0.42565159200477115	No Hit
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	1943	0.38755437828738065	TruSeq Adapter, Index 19 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTC	1637	0.32651905159878647	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTA	1137	0.22678812563703127	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCT	999	0.19926239007158683	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT	881	0.17572589154461263	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCTGAACACTCGTATGCCG	845	0.16854526487536625	No Hit
GAATCTTTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	718	0.14321360968108043	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	632	0.12605989041565854	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCC	568	0.11329433189255389	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGAACACTCGT	564	0.11249648448485984	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	527	0.10511639596368996	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	521	0.1039196248521489	No Hit
GAATCTGTCTCTTATCCACATCTGACGCCTGAACACTCGTATGCCGTCTTC	519	0.10352070114830188	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09115406632904424	0.0
2	0.0	0.0	0.0	0.6508440228264143	0.0
3	0.0	0.0	0.0	0.8429257862287548	0.0
4	0.0	0.0	0.0	1.7315283365479934	0.0
5	0.0	0.0	0.0	3.822287468410229	0.0
6	0.0	0.0	0.0	4.42007463862499	0.0
7	0.0	0.0	0.0	4.992729615497388	0.0
8	0.0	0.0	0.0	5.74170886947017	0.0
9	0.0	0.0	0.0	6.078400475517055	0.0
10	0.0	0.0	0.0	8.89579913393664	0.0
11	0.0	0.0	0.0	9.466858415993649	0.0
12	0.0	0.0	0.0	11.510145627098089	0.0
13	0.0	0.0	0.0	11.785004059048687	0.0
14	0.0	0.0	0.0	11.95115578170097	0.0
15	0.0	3.9892370384702073E-4	0.0	12.367831590369184	0.0
16	0.0	3.9892370384702073E-4	0.0	12.629326078240906	0.0
17	0.0	3.9892370384702073E-4	0.0	12.85631366572986	0.0
18	0.0	3.9892370384702073E-4	0.0	13.080309325439963	0.0
19	0.0	3.9892370384702073E-4	0.0	13.795380064585748	0.0
20	0.0	3.9892370384702073E-4	0.0	14.01319240688622	0.0
21	0.0	3.9892370384702073E-4	0.0	14.222028965850136	0.0
22	0.0	3.9892370384702073E-4	0.0	14.531793221887348	0.0
23	0.0	3.9892370384702073E-4	0.0	14.772344215307102	0.0
24	0.0	3.9892370384702073E-4	0.0	14.960237279819049	0.0
25	0.0	3.9892370384702073E-4	0.0	15.114620753207845	0.0
26	0.0	3.9892370384702073E-4	0.0	15.27339238733896	0.0
27	0.0	3.9892370384702073E-4	0.0	15.457296214812436	0.0
28	0.0	3.9892370384702073E-4	0.0	15.623647399316644	0.0
29	0.0	3.9892370384702073E-4	0.0	15.804559299011268	0.0
30	0.0	3.9892370384702073E-4	0.0	15.996840524265531	0.0
31	1.9946185192351037E-4	3.9892370384702073E-4	0.0	16.176755114700537	0.0
32	1.9946185192351037E-4	3.9892370384702073E-4	0.0	16.338518676610505	0.0
33	1.9946185192351037E-4	3.9892370384702073E-4	0.0	16.523818737047446	0.0
34	1.9946185192351037E-4	3.9892370384702073E-4	0.0	16.71330749637478	0.0
35	1.9946185192351037E-4	3.9892370384702073E-4	0.0	16.923340826450236	0.0
36	1.9946185192351037E-4	3.9892370384702073E-4	0.0	17.097471023179462	0.0
37	1.9946185192351037E-4	3.9892370384702073E-4	0.0	17.282771083616403	0.0
38	1.9946185192351037E-4	3.9892370384702073E-4	0.0	17.485823248874535	0.0
39	1.9946185192351037E-4	3.9892370384702073E-4	0.0	17.799377280098295	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATT	20	7.029478E-4	45.0	22
CGCATTT	25	3.8876486E-5	45.0	23
TACGAAA	45	3.8380676E-10	45.0	20
TAGGGCG	25	3.8876486E-5	45.0	5
TTCGGTC	25	3.8876486E-5	45.0	10
TATACGG	40	6.8012014E-9	45.0	27
CCACCGT	25	3.8876486E-5	45.0	15
CGTTTTT	18775	0.0	44.64048	1
CGACCAC	800	0.0	42.46875	12
TACGGCT	900	0.0	42.0	7
GATACCT	1325	0.0	41.773586	5
GAATGAT	3975	0.0	41.03774	1
TGATACC	1360	0.0	41.029415	4
GCGACCA	835	0.0	40.958084	11
ACGGCTG	925	0.0	40.864864	8
ATGATAC	3745	0.0	40.79439	3
GATACGG	2405	0.0	40.602913	5
AATGATA	3735	0.0	40.60241	2
ACCACCG	455	0.0	40.54945	14
GAATCTG	1155	0.0	40.519478	1
>>END_MODULE