##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933359.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	296484
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.74508573818486	31.0	28.0	31.0	16.0	33.0
2	29.20886455930168	31.0	30.0	33.0	16.0	34.0
3	29.443187490724625	31.0	30.0	33.0	16.0	34.0
4	32.16633949892743	35.0	30.0	37.0	19.0	37.0
5	33.55282578486529	35.0	33.0	37.0	28.0	37.0
6	33.007578823815116	35.0	33.0	37.0	25.0	37.0
7	31.684283131636107	35.0	30.0	35.0	23.0	37.0
8	33.054731452624765	35.0	32.0	35.0	28.0	37.0
9	32.55548697400197	35.0	31.0	37.0	25.0	39.0
10	30.33215957690803	34.0	27.0	37.0	15.0	39.0
11	31.14182890139097	35.0	27.0	39.0	15.0	39.0
12	32.16503420083377	35.0	30.0	39.0	18.0	39.0
13	32.269609827174484	35.0	30.0	39.0	18.0	39.0
14	32.76550505254921	36.0	30.0	39.0	17.0	41.0
15	33.35539185925716	36.0	30.0	39.0	21.0	41.0
16	33.57077616330055	36.0	31.0	39.0	22.0	41.0
17	31.55469097826527	34.0	27.0	39.0	17.0	41.0
18	32.39749531172003	35.0	29.0	39.0	19.0	39.0
19	32.25858393707586	35.0	30.0	37.0	18.0	39.0
20	32.23248472092929	34.0	30.0	37.0	23.0	39.0
21	32.60411691693312	35.0	31.0	37.0	23.0	39.0
22	33.05603000499184	35.0	31.0	38.0	25.0	39.0
23	34.166204584395786	35.0	33.0	38.0	27.0	40.0
24	32.30564549857665	35.0	30.0	37.0	21.0	39.0
25	31.475303894982527	34.0	29.0	37.0	18.0	39.0
26	31.016675436111225	33.0	27.0	37.0	20.0	39.0
27	32.448213057028376	35.0	30.0	38.0	22.0	39.0
28	32.509653134739146	35.0	31.0	38.0	21.0	39.0
29	32.91208294545405	35.0	31.0	38.0	22.0	40.0
30	31.79818472497673	35.0	30.0	38.0	18.0	40.0
31	31.91970224362866	35.0	30.0	37.0	21.0	40.0
32	31.8449865760041	35.0	30.0	38.0	18.0	40.0
33	32.077343128128334	35.0	30.0	38.0	18.0	40.0
34	31.493213799058296	35.0	30.0	38.0	15.0	40.0
35	31.048562485665332	35.0	29.0	38.0	12.0	40.0
36	30.38691801243912	35.0	27.0	38.0	10.0	40.0
37	30.633167388459412	35.0	29.0	38.0	10.0	40.0
38	30.489240566101376	35.0	27.0	38.0	10.0	40.0
39	30.04990825811848	34.0	26.0	38.0	9.0	40.0
40	29.807881706938655	34.0	25.0	38.0	8.0	40.0
41	29.158295219978143	33.0	23.0	37.0	8.0	40.0
42	29.785054168184455	34.0	25.0	38.0	8.0	40.0
43	29.72424481590912	34.0	24.0	38.0	8.0	40.0
44	29.795796737766626	34.0	25.0	38.0	8.0	40.0
45	30.04162450587553	35.0	26.0	38.0	8.0	40.0
46	29.89292845482387	34.0	25.0	38.0	8.0	40.0
47	29.982521822425493	34.0	26.0	38.0	7.0	40.0
48	29.671247015015986	34.0	25.0	37.0	8.0	40.0
49	29.469070843620568	34.0	26.0	37.0	7.0	39.0
50	29.623622185345585	34.0	26.0	37.0	7.0	40.0
51	27.935719971398118	32.0	23.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	17.0
10	23.0
11	23.0
12	37.0
13	56.0
14	82.0
15	169.0
16	328.0
17	560.0
18	962.0
19	1518.0
20	2323.0
21	3365.0
22	4461.0
23	6125.0
24	8154.0
25	11266.0
26	14062.0
27	15502.0
28	16227.0
29	17102.0
30	19576.0
31	22238.0
32	24645.0
33	27736.0
34	30338.0
35	32046.0
36	23416.0
37	11723.0
38	2346.0
39	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.10164460814074	2.873342237692422	40.79714251021977	35.22787064394706
2	36.31595634165756	8.717502462190202	39.968767285924365	14.99777391022787
3	15.10401910389768	8.673992525734947	60.8933365712821	15.32865179908528
4	13.678984363405783	3.252114785283523	64.50263757909364	18.56626327221705
5	18.006367965893606	3.7624290012277224	60.0302208550883	18.20098217779037
6	16.319599034011954	8.399104167509883	61.86910592139879	13.41219087707937
7	60.7941743905236	1.3990636931503893	34.853145532305284	2.9536163840207226
8	62.59494610164461	5.705872829562472	28.532062438445244	3.167118630347675
9	58.36503824826972	2.836915314148487	28.94759919590939	9.850447241672402
10	33.96574520041554	17.565534733746173	35.26699585812388	13.201724207714413
11	25.966325332901608	17.741598197541858	41.65452435881869	14.637552110737847
12	22.20693190863588	15.38093118009741	45.52758327599466	16.884553635272052
13	19.67222514537041	16.11014422363433	49.606386853928036	14.611243777067228
14	17.182714750205744	16.705791880843485	48.11355756128493	17.997935807665844
15	14.846669634786364	15.794106933257781	52.35560772250779	17.003615709448063
16	18.38008121854805	15.48886280541277	45.45877686485611	20.672279111183066
17	19.02193710284535	15.973543260344572	46.472322283833186	18.53219735297689
18	18.743001308670955	15.428151266172879	46.56979803294613	19.259049392210034
19	18.785836672467994	17.944644567666383	43.982474602339416	19.287044157526207
20	19.15550248917311	19.657721833218655	46.537081259022415	14.649694418585826
21	19.794997369166634	19.597010293978766	44.67930815828173	15.928684178572874
22	17.761835377288488	16.39144102211249	46.66052805547686	19.186195545122164
23	17.96791732437501	19.963977820051	45.37985186384426	16.688252991729737
24	17.679200226656413	18.352086453231877	44.2354393491723	19.73327397093941
25	17.900460058552905	19.91810687929197	42.73518975728876	19.446243304866368
26	16.90108066539847	18.052576192981746	44.311328773222165	20.73501436839762
27	17.30582426033108	17.95611230285614	44.63748465347203	20.100578783340755
28	15.000134914531642	19.09479094993322	46.241281148392495	19.663792987142646
29	18.780777377531336	18.770321501328908	43.51196017323026	18.936940947909502
30	19.2546646699316	18.966284858542114	44.17371595094507	17.605334520581213
31	19.618259332712725	18.165904399562876	43.72613699221543	18.489699275508965
32	21.050714372445057	19.52786659651111	41.54996559679443	17.871453434249403
33	20.161627608909754	18.130489335006274	39.58999473833327	22.117888317750705
34	20.261127075997358	21.78869686053885	39.72153640668636	18.228639656777432
35	19.73226211195208	22.91118576381862	37.295773127723585	20.06077899650571
36	20.834513835485218	22.438984903063908	37.74706223607345	18.979439025377424
37	20.78155988181487	22.268992593192213	38.55789857125511	18.391548953737807
38	20.433817676501935	21.261181041810012	37.46272986063329	20.84227142105476
39	21.682114380539925	21.257808178518907	36.86505848544947	20.195018955491694
40	22.095964706358522	20.533654429918645	35.612039772803925	21.758341090918904
41	20.655077508398428	21.812306903576584	36.86033647684192	20.672279111183066
42	19.17607695524885	21.06015838966015	38.56498158416643	21.19878307092457
43	20.149148014732667	20.623709879791154	37.72480133835215	21.502340767124025
44	22.14048650180111	21.082419287381445	35.407306971033854	21.369787239783598
45	19.70055719701569	20.624384452449373	35.261261990529	24.413796360005936
46	20.95391319599034	22.26730616154666	35.59348902470285	21.18529161776015
47	17.812765612984176	21.363041513201388	39.97922316212679	20.844969711687646
48	20.354555389160968	23.024513970399752	35.42720686445137	21.19372377598791
49	19.73968241119251	20.929291293965274	37.58685122974595	21.74417506509626
50	19.499534544865828	20.916137127129964	36.299092025202036	23.285236302802176
51	19.65401168359844	21.37754482535314	34.731047881167285	24.23739560988114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	84.0
1	348.0
2	612.0
3	11900.0
4	23188.0
5	15242.0
6	7296.0
7	6937.5
8	6579.0
9	6496.0
10	6413.0
11	6037.0
12	5661.0
13	5238.5
14	4816.0
15	4453.0
16	4090.0
17	3706.5
18	3323.0
19	3068.0
20	2813.0
21	2768.0
22	2723.0
23	2535.5
24	2348.0
25	2412.0
26	2722.5
27	2969.0
28	3186.0
29	3403.0
30	3628.5
31	3854.0
32	4248.5
33	4643.0
34	5108.0
35	5573.0
36	5953.0
37	6333.0
38	6555.5
39	6778.0
40	7460.0
41	8142.0
42	8947.0
43	9752.0
44	10818.5
45	11885.0
46	18837.0
47	25789.0
48	23064.5
49	20340.0
50	19396.0
51	18452.0
52	17016.0
53	15580.0
54	14872.0
55	14164.0
56	14041.5
57	13919.0
58	12972.5
59	12026.0
60	10897.0
61	9768.0
62	8889.0
63	8010.0
64	7264.5
65	6519.0
66	5718.5
67	4918.0
68	4252.0
69	3586.0
70	3106.5
71	2627.0
72	2287.5
73	1948.0
74	1676.5
75	1128.0
76	851.0
77	647.0
78	443.0
79	332.5
80	222.0
81	167.0
82	112.0
83	68.0
84	24.0
85	19.5
86	15.0
87	10.5
88	6.0
89	5.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	296484.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.06046303184055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5168557022284	38.52073296555535
2	7.709430495464496	7.564564596385602
3	3.8664451594399947	5.69068769428034
4	2.5723772817900903	5.048080821488368
5	1.786853777105591	4.383193683749674
6	1.3360738162868802	3.9329040043051564
7	1.0060344292692185	3.454956044814491
8	0.7607045874206544	2.9856415431442023
9	0.5751096427484316	2.5393630830583005
>10	1.8032017867059866	12.737054834011596
>50	0.0383357571324348	1.268664851573026
>100	0.02300145427946088	2.079447698764305
>500	0.002788055064177076	0.9923626413112997
>1k	0.002091041298132807	3.091301956393573
>5k	0.0	0.0
>10k+	6.97013766044269E-4	5.711043581164719
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16701	5.633018982474603	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCG	3783	1.2759541830250536	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGC	2907	0.9804913587242482	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTC	2350	0.7926228734096951	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	797	0.2688172043010753	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	775	0.26139690506064406	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTCGCTT	740	0.2495918835417763	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTA	590	0.1989989341752	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTC	452	0.15245342075794985	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	367	0.12378408278355663	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	350	0.11805021518867798	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC	349	0.11771292885956745	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09713846278382644	0.0
2	0.0	0.0	0.0	0.5474157121463553	0.0
3	0.0	0.0	0.0	0.6867149660689953	0.0
4	0.0	0.0	0.0	1.1902834554309845	0.0
5	0.0	0.0	0.0	2.3397552650395976	0.0
6	0.0	0.0	0.0	2.63994009794795	0.0
7	0.0	0.0	0.0	2.998812752121531	0.0
8	0.0	0.0	0.0	3.469664467559801	0.0
9	0.0	0.0	0.0	3.6838412865449737	0.0
10	0.0	0.0	0.0	5.6424629996896964	0.0
11	0.0	0.0	0.0	6.399670808542788	0.0
12	0.0	0.0	0.0	8.042929803969185	0.0
13	0.0	0.0	0.0	8.30432670902983	0.0
14	0.0	0.0	0.0	8.454756411813117	0.0
15	0.0	0.0	0.0	8.925945413580497	0.0
16	0.0	0.0	0.0	9.180596592058931	0.0
17	0.0	0.0	0.0	9.469313689777525	0.0
18	0.0	0.0	0.0	9.738130894078601	0.0
19	0.0	0.0	0.0	10.279475452300968	0.0
20	0.0	0.0	0.0	10.561446823437352	0.0
21	0.0	0.0	0.0	10.875460395839236	0.0
22	0.0	0.0	0.0	11.303814033809582	0.0
23	0.0	0.0	0.0	11.597590426464835	0.0
24	0.0	0.0	0.0	11.856963613550816	0.0
25	0.0	0.0	0.0	12.07856073177642	0.0
26	0.0	0.0	0.0	12.272500371014962	0.0
27	0.0	0.0	0.0	12.535246421392047	0.0
28	0.0	0.0	0.0	12.7423402274659	0.0
29	0.0	0.0	0.0	12.97405593556482	0.0
30	0.0	0.0	0.0	13.270530618852957	0.0
31	0.0	0.0	0.0	13.480660001888804	0.0
32	0.0	0.0	0.0	13.692138530241092	0.0
33	0.0	0.0	0.0	13.905640776568044	0.0
34	0.0	0.0	0.0	14.184576570742434	0.0
35	0.0	0.0	0.0	14.496566425169656	0.0
36	0.0	0.0	0.0	14.757963330230298	0.0
37	0.0	0.0	0.0	15.00418235048097	0.0
38	0.0	0.0	0.0	15.321568786173959	0.0
39	0.0	0.0	0.0	15.996141444394976	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGGG	30	2.1600335E-6	45.000004	3
TTTACGG	20	7.0248084E-4	45.0	2
TAGCGGG	35	1.2078453E-7	45.0	3
GGCAACG	20	7.0248084E-4	45.0	8
GGATACC	20	7.0248084E-4	45.0	8
TCGGAAC	20	7.0248084E-4	45.0	25
GGGCGTA	20	7.0248084E-4	45.0	7
CGTGTTT	20	7.0248084E-4	45.0	1
CCGTTGG	35	1.2078453E-7	45.0	2
CGTTTTT	6865	0.0	44.344498	1
TCGTTTG	120	0.0	43.125004	1
TACGGGG	80	0.0	42.1875	4
TGATACC	395	0.0	42.151897	4
GATACCT	395	0.0	41.582275	5
CGGGATA	55	6.002665E-11	40.909092	6
CTACGGG	50	1.0750227E-9	40.5	3
GGGTAAT	45	1.9199433E-8	40.000004	7
TACGGCT	400	0.0	39.9375	7
ACGGCTG	395	0.0	39.873417	8
GTTTTTT	7820	0.0	39.763428	2
>>END_MODULE