##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933358.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285645
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.668956221883807	31.0	28.0	33.0	25.0	34.0
2	29.06082024891036	31.0	28.0	33.0	16.0	34.0
3	29.06184599765443	31.0	28.0	34.0	16.0	34.0
4	33.343076195977524	35.0	32.0	37.0	28.0	37.0
5	34.34295716711303	35.0	33.0	37.0	31.0	37.0
6	33.87090269390327	35.0	33.0	37.0	28.0	37.0
7	33.64312345743843	35.0	32.0	37.0	29.0	37.0
8	33.75038246774843	35.0	33.0	37.0	28.0	37.0
9	34.777503544609566	37.0	34.0	39.0	28.0	39.0
10	34.64841324021075	37.0	33.0	39.0	27.0	39.0
11	34.93748183934604	37.0	34.0	39.0	27.0	39.0
12	34.988723765513136	37.0	34.0	39.0	27.0	39.0
13	35.189893049064395	37.0	34.0	39.0	29.0	39.0
14	35.88035148523517	38.0	34.0	40.0	27.0	41.0
15	36.27546430009277	38.0	34.0	40.0	30.0	41.0
16	36.24543051690035	38.0	34.0	40.0	30.0	41.0
17	35.543737856430184	38.0	33.0	40.0	27.0	41.0
18	35.38623116105656	37.0	33.0	39.0	27.0	40.0
19	34.72983248437746	37.0	33.0	38.0	27.0	40.0
20	34.26281223196625	35.0	33.0	38.0	27.0	40.0
21	34.51503089499204	35.0	33.0	39.0	27.0	40.0
22	35.10862784225175	35.0	33.0	39.0	30.0	40.0
23	35.49292303383571	36.0	34.0	39.0	30.0	40.0
24	34.946366993996044	35.0	34.0	39.0	29.0	40.0
25	33.80319627509671	35.0	33.0	39.0	24.0	40.0
26	34.0089726758739	35.0	33.0	39.0	25.0	40.0
27	34.75258450174167	35.0	33.0	39.0	27.0	40.0
28	34.614598540145984	35.0	33.0	39.0	27.0	40.0
29	34.62311960650458	35.0	33.0	39.0	25.0	41.0
30	33.860911270983216	35.0	33.0	39.0	23.0	40.0
31	33.88532269075251	35.0	33.0	39.0	23.0	40.0
32	33.365597157310646	35.0	33.0	39.0	21.0	40.0
33	32.85489681247702	36.0	32.0	39.0	15.0	41.0
34	32.43395823487196	36.0	31.0	40.0	12.0	41.0
35	32.028041800136535	36.0	31.0	40.0	10.0	41.0
36	31.502067251308443	36.0	30.0	40.0	8.0	41.0
37	31.355332668172032	36.0	29.0	40.0	8.0	41.0
38	31.070065991002817	35.0	27.0	40.0	8.0	41.0
39	30.715041397538904	35.0	26.0	39.0	7.0	40.0
40	30.5298849971118	35.0	24.0	39.0	7.0	41.0
41	30.19590400672163	35.0	23.0	39.0	7.0	40.0
42	30.36422132367099	35.0	23.0	39.0	7.0	40.0
43	30.22720509723608	35.0	23.0	39.0	7.0	40.0
44	30.298412364998512	36.0	23.0	39.0	7.0	40.0
45	30.36392375150974	36.0	23.0	39.0	7.0	40.0
46	30.163185072380053	35.0	23.0	39.0	7.0	40.0
47	30.08688757023578	35.0	23.0	39.0	7.0	40.0
48	29.953302175777626	35.0	23.0	39.0	7.0	40.0
49	30.154523972063224	35.0	24.0	39.0	7.0	40.0
50	30.119161896759966	35.0	24.0	39.0	7.0	40.0
51	29.028454900313324	34.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	12.0
11	10.0
12	18.0
13	33.0
14	31.0
15	54.0
16	127.0
17	224.0
18	479.0
19	840.0
20	1424.0
21	2134.0
22	3131.0
23	4672.0
24	6684.0
25	9720.0
26	12674.0
27	13532.0
28	12769.0
29	11950.0
30	12018.0
31	13281.0
32	15566.0
33	18671.0
34	21870.0
35	26244.0
36	30343.0
37	33842.0
38	28671.0
39	4612.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.13434507868158	2.648042150221429	36.142414535524864	36.075198235572124
2	35.759071574856904	14.378336746661066	35.85114390239633	14.011447776085701
3	12.41261005793905	14.478461026799	58.953596247089926	14.155332668172033
4	11.15370477340755	2.887850303698647	69.75511561553677	16.203329307357034
5	20.451959600203047	3.4122774772882427	59.56344413520279	16.572318787305925
6	14.62374625846768	12.792802254546729	61.02014738574104	11.563304101244553
7	56.87514222198883	0.9487300670412575	39.70347809343766	2.472649617532251
8	57.03618127395893	9.091354653503474	31.266082024891034	2.6063820476465542
9	53.837455582978876	2.9081552276427036	30.570813422254894	12.683575767123529
10	30.49484499991248	22.90780514274712	35.85779551541249	10.739554341927917
11	22.086856062595178	17.521398939242765	46.894922018589504	13.496822979572546
12	17.920145635316565	16.39727633951233	47.97598417616272	17.706593849008385
13	17.60927024803515	17.205972448318718	52.75569325561449	12.429064048031648
14	15.339669869943462	22.117313448511265	46.23536207530326	16.307654606242014
15	12.488578480281468	17.465735440844405	54.64860228605437	15.39708379281976
16	15.285756796023037	19.291078086435963	45.44942148471004	19.973743632830963
17	15.800031507640602	18.83561763727704	49.93400899718182	15.43034185790054
18	15.679952388454199	17.453482469498855	49.099756690997566	17.76680845104938
19	14.973831154054858	19.79765093035061	46.6330585166903	18.595459398904236
20	17.16431234574384	19.7542404032978	50.44653328432145	12.63491396663691
21	16.994871256279648	23.06499326086576	46.97789213884367	12.962243344010924
22	15.371177510546307	16.5810709096956	49.62418386458716	18.42356771517093
23	14.898562901503615	22.07915419489226	48.663900995991526	14.358381907612596
24	16.47429501654151	18.432669922456196	47.038106740884665	18.05492832011763
25	14.153932328589683	25.67137530851231	44.752052372700376	15.422639990197624
26	14.636699399604405	19.383850583766563	47.730574664356105	18.248875352272925
27	16.424933046263718	20.71977454532724	45.13399499378599	17.721297414623045
28	13.218505487580739	20.916172171751647	48.95097061037302	16.914351730294598
29	16.945159201106268	20.710322253146387	45.38570603371318	16.958812512034168
30	15.2840063715451	24.378861874004446	46.738784155157624	13.598347599292829
31	20.136533109279	18.909485550245936	43.148663550911095	17.80531778956397
32	21.34292565947242	23.10105200511124	41.55752770046736	13.998494634948974
33	16.822279402755168	23.110504297292092	39.64991510441282	20.41730119553992
34	18.65812459521434	24.395665948992633	39.62330865234819	17.322900803444835
35	18.26217857830524	26.60260113077421	37.595266852211665	17.539953438708885
36	19.332388104115246	28.336571618617512	35.47165187557983	16.859388401687408
37	19.642913406501076	26.29662693203102	37.477288242398785	16.583171419069124
38	20.8874652103135	27.088518965849218	34.82679549790824	17.197220325929038
39	21.094015298709937	24.43802622135868	35.08830891491186	19.379649565019516
40	21.438148751072138	25.795305361550174	36.28489908802885	16.48164679934884
41	18.309790124105096	28.222093857760505	33.672915682052896	19.7952003360815
42	20.431304591363407	23.95981025398659	38.040224754502965	17.568660400147035
43	19.58549948362478	26.566542386528734	34.182639290027836	19.665318839818656
44	20.37984211171209	24.828720964833973	34.65350347459259	20.137933448861347
45	18.854522221638746	23.23898545397259	34.80368989479949	23.102802429589175
46	22.038894431899735	25.680827600693167	33.26261618442472	19.017661782982373
47	16.08850146160444	24.809466295576676	40.67671410317002	18.425318139648862
48	18.894431899735686	25.761697211573807	33.8175007439304	21.526370144760104
49	18.32554394440652	22.94071312293231	38.84542001435348	19.88832291830769
50	18.496035288557476	23.724903289047596	36.56601725918535	21.21304416320958
51	18.03007229253094	22.69040242258748	35.5763272593604	23.70319802552119
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17.0
1	399.5
2	782.0
3	9252.0
4	17722.0
5	13133.5
6	8545.0
7	8203.0
8	7861.0
9	7580.0
10	7299.0
11	6862.5
12	6426.0
13	6001.5
14	5577.0
15	5182.0
16	4787.0
17	4368.0
18	3949.0
19	3708.5
20	3468.0
21	3299.0
22	3130.0
23	3055.0
24	2980.0
25	3210.0
26	3750.0
27	4060.0
28	4449.5
29	4839.0
30	5213.5
31	5588.0
32	6364.5
33	7141.0
34	7541.0
35	7941.0
36	8179.5
37	8418.0
38	9110.0
39	9802.0
40	10451.5
41	11101.0
42	11549.5
43	11998.0
44	12518.0
45	13038.0
46	25841.5
47	38645.0
48	28267.0
49	17889.0
50	16505.5
51	15122.0
52	13615.5
53	12109.0
54	11463.0
55	10817.0
56	9989.5
57	9162.0
58	8120.5
59	7079.0
60	6155.0
61	5231.0
62	4556.0
63	3881.0
64	3094.5
65	2308.0
66	1847.5
67	1387.0
68	1087.5
69	788.0
70	661.0
71	534.0
72	415.0
73	296.0
74	250.0
75	168.5
76	133.0
77	106.0
78	79.0
79	54.5
80	30.0
81	26.5
82	23.0
83	17.5
84	12.0
85	8.5
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	285645.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.35325801705718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.24246835410143	39.90116454942558
2	7.393809129388113	7.446047576419051
3	4.096899684915091	6.188767407135896
4	2.7233764535475586	5.485235089722335
5	1.8750928627644523	4.720851736236043
6	1.3512079334554918	4.082263302478741
7	0.9633655573029359	3.3956016130134574
8	0.6500928629980915	2.6187434932472247
9	0.4249002162161528	1.925559919677179
>10	1.2205972174966035	9.220886699693128
>50	0.03295080972604636	1.1614264368045986
>100	0.017527026450024658	1.603050896513581
>500	7.010810580009863E-4	0.2238128756638649
>1k	0.004206486348005918	2.6006773738417865
>5k	0.002804324232003945	9.425911030127535
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8469	2.9648689807278266	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCG	6535	2.2878047926622203	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTC	6003	2.101559628209841	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGC	5694	1.9933833954734022	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCT	1423	0.498170806420557	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGGACGAT	1312	0.45931138301038	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTC	1225	0.4288539970942954	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	1164	0.40749881846347746	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGGACGATCGTA	1162	0.406798648672303	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCT	1081	0.37844177212974145	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTT	634	0.22195382380227205	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGGACGATCGT	499	0.17469236289800277	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATG	286	0.10012428013793345	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05461324371160006	0.0
2	0.0	0.0	0.0	0.560485917835075	0.0
3	0.0	0.0	0.0	0.6511579057921546	0.0
4	0.0	0.0	0.0	1.2540040959932783	0.0
5	0.0	0.0	0.0	3.735055751019622	0.0
6	0.0	0.0	0.0	4.052232666421607	0.0
7	0.0	0.0	0.0	4.556354916067146	0.0
8	0.0	0.0	0.0	5.125592956291901	0.0
9	0.0	0.0	0.0	5.216965114040155	0.0
10	0.0	0.0	0.0	8.008542071452327	0.0
11	0.0	0.0	0.0	8.205989952563497	0.0
12	0.0	0.0	0.0	10.645381505014965	0.0
13	0.0	0.0	0.0	10.84843074445553	0.0
14	0.0	0.0	0.0	11.00981988132122	0.0
15	0.0	0.0	0.0	11.543349262196083	0.0
16	0.0	0.0	0.0	11.815015141171735	0.0
17	0.0	0.0	0.0	11.958900033258065	0.0
18	0.0	0.0	0.0	12.080729576922403	0.0
19	0.0	0.0	0.0	12.748691557702744	0.0
20	0.0	0.0	0.0	12.902378826865515	0.0
21	0.0	0.0	0.0	13.025258625216615	0.0
22	0.0	0.0	0.0	13.241611090689492	0.0
23	0.0	0.0	0.0	13.38584606767141	0.0
24	0.0	0.0	0.0	13.501374083215179	0.0
25	0.0	0.0	0.0	13.595196835232544	0.0
26	0.0	0.0	0.0	13.71597612421012	0.0
27	0.0	0.0	0.0	13.867912968894958	0.0
28	0.0	0.0	0.0	13.976089201631396	0.0
29	0.0	0.0	0.0	14.112622310910396	0.0
30	0.0	0.0	0.0	14.263858985804058	0.0
31	0.0	0.0	0.0	14.384638274781635	0.0
32	0.0	0.0	0.0	14.536575119466471	0.0
33	0.0	0.0	0.0	14.67205797405871	0.0
34	0.0	0.0	0.0	14.847800591643473	0.0
35	0.0	0.0	0.0	15.039297029529662	0.0
36	0.0	0.0	0.0	15.21433947732325	0.0
37	0.0	0.0	0.0	15.3932328589683	0.0
38	0.0	0.0	0.0	15.576677344255982	0.0
39	0.0	0.0	0.0	15.840991440424302	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	20	7.024374E-4	45.000004	31
CGACCCT	20	7.024374E-4	45.000004	12
TGTTGCG	40	6.7848305E-9	45.000004	1
GCGATTA	20	7.024374E-4	45.000004	9
CGGGACG	20	7.024374E-4	45.000004	6
CACCCGT	40	6.7848305E-9	45.000004	16
TTCCGCG	25	3.883421E-5	45.0	1
CGGGCAA	25	3.883421E-5	45.0	6
ACGTAGA	25	3.883421E-5	45.0	15
CCCTCGG	30	2.1597534E-6	44.999996	2
TCCGCGG	30	2.1597534E-6	44.999996	2
CGTTTTT	6520	0.0	44.58589	1
CCGATGA	185	0.0	43.783783	18
CGATGAA	195	0.0	42.692307	19
TGATACC	740	0.0	42.567566	4
GGCACCG	85	0.0	42.35294	8
GCACCGA	85	0.0	42.35294	9
GATACCT	760	0.0	41.743423	5
GCGACCT	60	3.6379788E-12	41.249996	11
ACGGCTG	695	0.0	41.11511	8
>>END_MODULE