##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933357.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	192132
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.837283742427083	31.0	28.0	31.0	16.0	33.0
2	29.17927778818729	31.0	30.0	33.0	16.0	34.0
3	29.357233568588263	31.0	30.0	33.0	16.0	34.0
4	32.392797659942126	35.0	32.0	37.0	19.0	37.0
5	33.79190868777715	35.0	33.0	37.0	30.0	37.0
6	33.0233225074428	35.0	33.0	37.0	25.0	37.0
7	32.14389065850561	35.0	31.0	35.0	25.0	37.0
8	33.208632606749525	35.0	33.0	35.0	28.0	37.0
9	32.92681593904191	35.0	31.0	37.0	25.0	39.0
10	31.49831365935919	35.0	27.0	39.0	15.0	39.0
11	32.46328565777694	35.0	30.0	39.0	17.0	39.0
12	33.16815002185997	35.0	31.0	39.0	23.0	39.0
13	33.30230778839548	35.0	32.0	39.0	25.0	39.0
14	33.2417504632232	36.0	31.0	40.0	17.0	41.0
15	34.04894551662399	36.0	32.0	40.0	25.0	41.0
16	34.3483386421835	37.0	32.0	40.0	25.0	41.0
17	32.409718318655926	36.0	27.0	39.0	18.0	41.0
18	33.15428455436887	36.0	30.0	39.0	23.0	39.0
19	32.99595590531509	36.0	31.0	37.0	21.0	39.0
20	32.76612953594404	35.0	31.0	37.0	25.0	39.0
21	32.95028417962651	35.0	32.0	37.0	24.0	39.0
22	33.29068557033706	35.0	31.0	38.0	26.0	39.0
23	34.35468844336186	35.0	33.0	38.0	28.0	40.0
24	32.50236295879916	35.0	31.0	37.0	22.0	39.0
25	31.455379634834383	35.0	29.0	37.0	18.0	39.0
26	31.0207617679512	33.0	27.0	37.0	20.0	39.0
27	32.77350467387005	35.0	31.0	38.0	23.0	40.0
28	32.834608498324066	35.0	31.0	38.0	22.0	39.0
29	33.115129182020695	35.0	31.0	38.0	22.0	40.0
30	31.823886702891762	35.0	30.0	38.0	18.0	40.0
31	32.3896435783732	35.0	30.0	38.0	21.0	40.0
32	32.264110090979116	35.0	30.0	38.0	20.0	40.0
33	32.29653571502925	35.0	31.0	38.0	18.0	40.0
34	31.466835300730747	35.0	30.0	38.0	15.0	40.0
35	30.895290737617888	35.0	29.0	38.0	10.0	40.0
36	30.261991755667978	35.0	26.0	38.0	8.0	40.0
37	30.31176482834718	35.0	27.0	38.0	8.0	40.0
38	30.260737409697498	35.0	25.0	38.0	8.0	40.0
39	29.875434597047864	35.0	25.0	38.0	8.0	40.0
40	29.694579768076114	35.0	24.0	38.0	8.0	40.0
41	29.380842337559596	34.0	23.0	38.0	8.0	40.0
42	29.77369204505236	35.0	24.0	38.0	8.0	40.0
43	29.686642516603168	35.0	23.0	38.0	7.0	40.0
44	29.626319405408783	35.0	23.0	38.0	7.0	40.0
45	29.78194158182916	35.0	23.0	38.0	7.0	40.0
46	29.5428039056482	35.0	23.0	38.0	7.0	40.0
47	29.607093040201526	35.0	23.0	38.0	7.0	40.0
48	29.34022442903837	34.0	23.0	38.0	7.0	40.0
49	29.094924322861367	34.0	23.0	38.0	7.0	40.0
50	29.326973122644848	34.0	24.0	38.0	7.0	40.0
51	27.850732829513042	33.0	21.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	8.0
11	4.0
12	8.0
13	16.0
14	31.0
15	87.0
16	174.0
17	314.0
18	581.0
19	909.0
20	1344.0
21	1908.0
22	2760.0
23	3771.0
24	5340.0
25	7269.0
26	9425.0
27	10350.0
28	10073.0
29	10115.0
30	11344.0
31	13151.0
32	15367.0
33	17711.0
34	19941.0
35	22184.0
36	16669.0
37	9133.0
38	2107.0
39	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.023046655424395	2.6507817542106467	37.986384360751984	38.33978722961297
2	39.249578414839796	9.760477171944288	37.31028667790894	13.679657735306977
3	12.982220556700602	9.663668727749672	63.449607561468156	13.904503154081569
4	11.685195594695314	2.9000895217871046	69.24041804592676	16.174296837590823
5	17.702412924447774	3.3742427081381554	62.88541211250599	16.037932254908082
6	14.711760664543128	9.162971290571067	63.80769470988695	12.317573334998855
7	56.5965065684009	0.9946286927737181	39.84864572273229	2.5602190160931024
8	58.43794891012429	6.351883080382237	32.423021672600086	2.787146336893386
9	54.457872712510145	2.8761476484916617	32.54481294110299	10.121166697895196
10	28.606374784002664	23.28867653488227	37.548664459850514	10.556284221264548
11	20.328732329856557	17.80078279516166	49.1297649532613	12.740719921720483
12	17.376595257427184	16.805113151375096	49.47900401807091	16.33928757312681
13	16.785855557637458	17.52753315428976	53.381529365228076	12.305081922844712
14	14.791393417025795	19.576645223075804	48.88930526929403	16.742656090604378
15	12.444569358565987	17.632148731080715	54.66450148855995	15.25878042179335
16	14.848645722732288	17.760185705660692	48.21476901297025	19.17639955863677
17	15.329044615160411	17.587908313034788	51.50469468906793	15.578352382736869
18	15.031332625486646	16.295047155080884	51.355838694231046	17.317781525201422
19	15.03445547852518	18.800616242999606	48.73368309287365	17.431245185601565
20	16.545395873670184	19.142568650719298	51.66760352257823	12.64443195303229
21	16.78481460662461	21.145878874940145	48.634792746653346	13.4345137717819
22	14.952740824016821	17.14706555909479	50.875439801802926	17.02475381508546
23	14.387504424041806	21.132866987279577	49.60600004163804	14.873628547040576
24	15.040180709095829	18.227052234921825	49.33066849874045	17.402098557241896
25	13.704640559615264	22.245643620011244	47.373680594591214	16.676035225782275
26	13.956030229217415	19.904024316615658	48.98090895842442	17.159036495742512
27	14.29485978389857	20.58844960756147	48.00033310432411	17.116357504215852
28	12.781317011221452	20.27252097516291	50.88584931193139	16.06031270168426
29	15.78185830574813	19.452251577040787	48.053942081485644	16.711948035725438
30	14.807007682218476	22.125413778027607	48.794578727125106	14.272999812628818
31	17.626423500510064	19.477234401349072	46.17658692981908	16.71975516832178
32	18.596069368975495	22.232111256844252	44.787437803176985	14.384381571003269
33	15.68348843503425	22.143109945245975	43.36133491557887	18.812066704140904
34	16.786896508650305	23.94395519746841	42.641517290196326	16.627631003684968
35	15.893240064122583	26.093519038994025	40.66058751275165	17.35265338413174
36	18.158869943580456	26.26007120104928	38.91543313971644	16.66562571565382
37	18.239023171569546	25.66672912372744	39.82158099639831	16.272666708304705
38	19.212832844086357	25.5074636187621	39.13195095038827	16.147752586763268
39	18.607519830116797	24.08552453521537	38.809776611912646	18.49717902275519
40	19.597984718839133	24.689276122665667	39.35367351612433	16.359065642370872
41	17.250119709366476	26.04563529240314	37.76362084400308	18.9406241542273
42	18.765223908562863	23.762309245726897	40.12033393708492	17.35213290862532
43	17.888222680240666	25.469468906793246	38.109737055774154	18.53257135719193
44	18.869319009847395	24.552911539982926	37.88645306351883	18.691316386650843
45	17.794016613578165	23.53694335144588	37.50494451731102	21.16409551766494
46	19.887889575916557	25.304478171257262	36.15587200466346	18.65176024816272
47	15.411800220681615	24.849062103137427	41.51156496575271	18.227572710428248
48	17.741969062935897	25.184768804780045	36.52801199175567	20.54525014052839
49	17.51764411966773	23.163762413340827	39.545208502487874	19.77338496450357
50	17.673266296088105	23.491141506880687	38.400162388358005	20.435429808673202
51	17.263652072533468	22.27062644431953	37.69231569962317	22.773405783523827
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	31.0
1	171.5
2	312.0
3	9177.5
4	18043.0
5	11996.0
6	5949.0
7	5820.0
8	5691.0
9	5526.5
10	5362.0
11	4945.0
12	4528.0
13	4112.5
14	3697.0
15	3331.5
16	2966.0
17	2696.0
18	2426.0
19	2292.5
20	2159.0
21	2041.5
22	1924.0
23	1879.5
24	1835.0
25	1943.5
26	2274.5
27	2497.0
28	2759.5
29	3022.0
30	3314.0
31	3606.0
32	4100.0
33	4594.0
34	4830.5
35	5067.0
36	5418.5
37	5770.0
38	6139.0
39	6508.0
40	7095.0
41	7682.0
42	8025.5
43	8369.0
44	8728.5
45	9088.0
46	13898.5
47	18709.0
48	15950.5
49	13192.0
50	12527.5
51	11863.0
52	10260.5
53	8658.0
54	7935.5
55	7213.0
56	6560.0
57	5907.0
58	5102.5
59	4298.0
60	3688.0
61	3078.0
62	2660.5
63	2243.0
64	1854.5
65	1466.0
66	1162.5
67	859.0
68	691.5
69	524.0
70	434.0
71	344.0
72	272.5
73	201.0
74	185.5
75	147.0
76	124.0
77	89.0
78	54.0
79	42.5
80	31.0
81	22.5
82	14.0
83	8.5
84	3.0
85	3.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	192132.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.15435554923395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.75676757447857	41.86265225562214
2	9.002973430590684	9.570945014598475
3	4.351275179408704	6.938676839365411
4	2.580999292131462	5.487654161851074
5	1.6919951897658456	4.496845695220367
6	1.1249966848541013	3.587908426706664
7	0.7849976834681881	2.920823218067515
8	0.4569986513172684	1.9433175038110782
9	0.323999043820085	1.5499764335542165
>10	0.8619974560889915	7.161996730350999
>50	0.03599989375777681	1.3724414099574178
>100	0.019999940976542674	1.8704962516034676
>500	0.002999991146481401	0.9163783852697862
>1k	0.002999991146481401	3.5974761667667714
>5k	0.0	0.0
>10k+	9.999970488271336E-4	6.722411507254633
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12647	6.582453729727479	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCG	2873	1.4953261299523244	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGC	2349	1.2225969645868466	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTC	1546	0.8046551329294443	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGGACGAT	626	0.32581766702059	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	582	0.3029167447379926	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCT	516	0.2685653613140966	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGGACGATCGTA	373	0.19413736389565509	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTC	343	0.17852309870297503	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCT	262	0.13636458268273893	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAAGGACGATCGTATGCCG	208	0.10825890533591488	No Hit
GAATCTTTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTC	200	0.10409510128453354	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10409510128453354	0.0
2	0.0	0.0	0.0	0.6334186913163866	0.0
3	0.0	0.0	0.0	0.8036141819165991	0.0
4	0.0	0.0	0.0	1.2668373826327732	0.0
5	0.0	0.0	0.0	2.431661566006704	0.0
6	0.0	0.0	0.0	2.7267711781483563	0.0
7	0.0	0.0	0.0	3.0458226635854517	0.0
8	0.0	0.0	0.0	3.4851039910061834	0.0
9	0.0	0.0	0.0	3.643849020465097	0.0
10	0.0	0.0	0.0	5.723148668623654	0.0
11	0.0	0.0	0.0	6.255595111694044	0.0
12	0.0	0.0	0.0	8.106926488039473	0.0
13	0.0	0.0	0.0	8.330210480294797	0.0
14	0.0	0.0	0.0	8.472820769054609	0.0
15	0.0	0.0	0.0	8.85745216830096	0.0
16	0.0	0.0	0.0	9.097911852268233	0.0
17	0.0	0.0	0.0	9.364395311556638	0.0
18	0.0	0.0	0.0	9.64961588907626	0.0
19	0.0	0.0	0.0	10.27782982532842	0.0
20	0.0	0.0	0.0	10.547956613161785	0.0
21	0.0	0.0	0.0	10.83005433764287	0.0
22	0.0	0.0	0.0	11.215206212395644	0.0
23	0.0	0.0	0.0	11.529052942768514	0.0
24	0.0	0.0	0.0	11.790852122499116	0.0
25	0.0	0.0	0.0	12.01621801678013	0.0
26	0.0	0.0	0.0	12.230133449919848	0.0
27	0.0	0.0	0.0	12.461745050277933	0.0
28	0.0	0.0	0.0	12.661607644744238	0.0
29	0.0	0.0	0.0	12.89217829408948	0.0
30	0.0	0.0	0.0	13.170632690025608	0.0
31	0.0	0.0	0.0	13.400162388358003	0.0
32	0.0	0.0	0.0	13.624487331626174	0.0
33	0.0	0.0	0.0	13.83840276476589	0.0
34	0.0	0.0	0.0	14.081985301771699	0.0
35	0.0	0.0	0.0	14.332854495867425	0.0
36	0.0	0.0	0.0	14.547290404513564	0.0
37	0.0	0.0	0.0	14.7846272354423	0.0
38	0.0	0.0	0.0	15.052672121249975	0.0
39	0.0	0.0	0.0	15.426894010367873	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTG	20	7.0186064E-4	45.000004	1
GGATGCA	30	2.1560372E-6	45.000004	8
GGAACGC	20	7.0186064E-4	45.000004	8
CGGTGTC	20	7.0186064E-4	45.000004	19
CTTCTCG	20	7.0186064E-4	45.000004	1
ATTTGCG	20	7.0186064E-4	45.000004	1
ACCGGTG	25	3.878644E-5	45.0	17
TTGTCGG	50	2.1827873E-11	45.0	2
CGCGCGG	25	3.878644E-5	45.0	2
ATATCAC	25	3.878644E-5	45.0	30
TCCGTTG	25	3.878644E-5	45.0	1
ACCACCG	105	0.0	45.0	14
CGATGAA	45	3.8198777E-10	45.0	19
CGTTTTT	5535	0.0	44.471542	1
CGACCAC	210	0.0	41.785713	12
TACGGCT	275	0.0	41.727272	7
TGATACC	335	0.0	41.641792	4
GTCGGGA	65	0.0	41.53846	4
GCGAGAC	60	3.6379788E-12	41.250004	21
CACGACC	60	3.6379788E-12	41.250004	27
>>END_MODULE