##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933356.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	657834
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.580401742688885	31.0	28.0	33.0	25.0	34.0
2	28.93771985029658	31.0	28.0	33.0	16.0	34.0
3	28.8719418576723	31.0	28.0	34.0	16.0	34.0
4	33.33734194340821	35.0	32.0	37.0	28.0	37.0
5	34.367919566334365	35.0	33.0	37.0	32.0	37.0
6	33.895870386754105	35.0	33.0	37.0	28.0	37.0
7	33.86466950628882	35.0	33.0	37.0	29.0	37.0
8	33.80539467403631	35.0	33.0	37.0	28.0	37.0
9	35.004917653997815	37.0	34.0	39.0	28.0	39.0
10	34.88500746388907	37.0	34.0	39.0	28.0	39.0
11	35.210119878267164	37.0	34.0	39.0	28.0	39.0
12	35.381869894228636	37.0	34.0	39.0	30.0	39.0
13	35.60934369460988	37.0	35.0	39.0	30.0	39.0
14	36.357958086690566	38.0	35.0	40.0	30.0	41.0
15	36.714839914020864	39.0	35.0	41.0	30.0	41.0
16	36.58747951610893	38.0	34.0	41.0	30.0	41.0
17	35.86254587023474	38.0	34.0	40.0	27.0	41.0
18	35.56513801354141	38.0	34.0	39.0	28.0	40.0
19	34.76055661458666	37.0	34.0	38.0	27.0	40.0
20	33.97196405172126	35.0	33.0	37.0	27.0	40.0
21	34.14761170751284	35.0	33.0	38.0	27.0	40.0
22	34.55906353274534	35.0	33.0	38.0	29.0	40.0
23	34.918979560192994	35.0	33.0	39.0	30.0	40.0
24	34.290026055205416	35.0	33.0	38.0	27.0	40.0
25	33.28168200488269	35.0	33.0	38.0	24.0	40.0
26	33.2466108471134	35.0	32.0	38.0	24.0	40.0
27	34.092184350459235	35.0	33.0	38.0	25.0	40.0
28	33.781324771902945	35.0	33.0	38.0	24.0	40.0
29	33.70003830753655	35.0	33.0	39.0	23.0	40.0
30	32.85236092996105	35.0	32.0	38.0	21.0	40.0
31	32.683441415311464	35.0	32.0	38.0	20.0	40.0
32	31.80805035920916	35.0	31.0	38.0	15.0	40.0
33	30.84937385419422	35.0	27.0	39.0	10.0	40.0
34	29.93875810614836	35.0	22.0	39.0	7.0	40.0
35	29.19756503920442	35.0	18.0	39.0	7.0	40.0
36	28.458737310628518	35.0	16.0	39.0	7.0	40.0
37	28.206877418923316	35.0	15.0	39.0	7.0	40.0
38	28.04545067600641	35.0	15.0	39.0	7.0	40.0
39	27.7188622053588	34.0	15.0	38.0	7.0	40.0
40	27.59280912813871	34.0	15.0	39.0	7.0	40.0
41	27.27531109672045	33.0	15.0	38.0	7.0	40.0
42	27.345419969171555	34.0	12.0	39.0	7.0	40.0
43	27.251294399498963	34.0	12.0	38.0	7.0	40.0
44	27.240142649969446	34.0	11.0	39.0	7.0	40.0
45	27.320301170204033	34.0	10.0	39.0	7.0	40.0
46	27.10470726657485	34.0	10.0	38.0	7.0	40.0
47	26.979683324364505	34.0	10.0	38.0	7.0	40.0
48	26.868287440296488	34.0	10.0	38.0	7.0	40.0
49	27.001404609673564	34.0	10.0	38.0	7.0	40.0
50	26.972407932700346	34.0	10.0	38.0	7.0	40.0
51	26.060250762350382	32.0	10.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	20.0
10	37.0
11	40.0
12	72.0
13	85.0
14	134.0
15	226.0
16	404.0
17	855.0
18	1710.0
19	3082.0
20	5100.0
21	7600.0
22	11203.0
23	16264.0
24	24333.0
25	35574.0
26	43886.0
27	42151.0
28	34351.0
29	28947.0
30	27747.0
31	29268.0
32	33082.0
33	38526.0
34	45138.0
35	53418.0
36	59802.0
37	61711.0
38	46505.0
39	6549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.76531769412952	2.18611382202805	29.882006706859176	45.16656177698325
2	44.863445793315634	13.311108881571949	28.84344682701107	12.981998498101344
3	10.404448538689092	13.635050787888739	62.75291334896037	13.207587324461795
4	8.901181757099815	2.4469699042615614	73.90314273813759	14.748705600501038
5	17.409863278577877	2.8639443993469476	64.96319740238418	14.762994919690986
6	11.41351769595369	11.430695281788415	67.3783963735532	9.777390648704689
7	47.10048431671212	0.8833535511998468	49.953787733683576	2.0623743984044607
8	46.63805762547998	8.033637665429271	42.758355451375266	2.5699492577154723
9	44.082549700988395	2.0584220335221346	41.808419753311625	12.050608512177845
10	25.06164169076089	13.067126357105288	49.52069975100101	12.35053220113281
11	20.227747425642335	14.951492321771145	51.898959311923676	12.921800940662841
12	14.199174867823796	13.920533143619817	56.25735367889163	15.62293830966475
13	14.019190251644032	14.618733601486088	60.775210767458056	10.586865379411828
14	13.529705062371358	18.863877513172017	54.419504008610076	13.186913415846552
15	11.287954103922875	14.36122182799916	60.94729065387317	13.4035334142048
16	12.405713295451436	16.516932843240088	53.32333080990037	17.754023051408105
17	12.779667818933044	16.820808897077377	56.71339578069847	13.686127503291104
18	14.215744397522778	15.520480850792145	55.12788940675003	15.135885344935044
19	13.492461624057134	17.663574701216415	52.617529650337325	16.226434024389132
20	15.525345299878085	16.724279985528266	56.88471559694391	10.865659117649741
21	14.855571466357775	20.24629313778248	53.95601321914039	10.942122176719355
22	13.959904778409143	13.563300163871128	56.0010580176762	16.475737040043537
23	13.895450828020442	19.261242197879707	54.91248552066327	11.93082145343658
24	15.038140321114446	16.285719497624022	53.493586527908256	15.182553653353278
25	12.575816999425388	22.746619967955443	51.638559271791976	13.0390037608272
26	13.455826241878649	15.378195715028411	54.83191808267739	16.334059960415544
27	15.812955852084265	17.158736094516247	51.74086471663064	15.287443336768849
28	11.398924348695871	18.316474976969875	54.81641265121596	15.468188023118296
29	14.894639073079228	16.395017588023727	51.285734699027415	17.424608639869632
30	13.374650747757034	21.389134644910417	52.591079208432525	12.645135398900026
31	15.429576458498648	18.92650729515349	49.244490251340004	16.39942599500786
32	16.050553787125626	21.546925212135584	48.35079974583253	14.051721254906251
33	17.590911992995192	17.985236397024174	44.03010485928061	20.393746750700025
34	16.30335312556055	20.286728870809352	43.5695023364557	19.840415667174394
35	15.480045117765274	23.770130458443926	42.90307281168197	17.84675161210883
36	19.486222968104418	23.853586163074574	37.07607086286206	19.58412000595895
37	16.583514990103886	26.769063319925696	39.50191081640657	17.145510873563847
38	21.025669089770368	25.225664833377415	36.67368971503449	17.074976361817722
39	20.454096322172465	23.78548387587142	35.731810760769434	20.02860904118668
40	21.228151782972603	22.53395233447952	40.0526576613553	16.18523822119258
41	20.76982947065673	24.864631502780338	34.96991642268414	19.39562260387879
42	18.446751004052693	23.780771440819418	40.563120787311085	17.209356767816804
43	22.560250762350382	22.01619253489482	35.42504644028737	19.99851026246743
44	20.891288683771286	23.76709017776521	34.83751219912622	20.50410893933728
45	18.709431254693435	22.85348583381218	35.942654225838126	22.49442868565626
46	21.9170793847688	24.28408990718023	34.08048231012687	19.718348397924096
47	17.71753968326356	22.63686583545393	40.745993670135626	18.899600811146886
48	19.360051319937856	24.49371725997744	33.97437651443981	22.17185490564489
49	18.187263048124603	22.403828321430634	38.95253209776327	20.456376532681496
50	19.30000577653329	21.8325595819006	37.05357278583959	21.813861855726522
51	18.73664176676791	21.737094768589035	34.72456577191206	24.801697692730993
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34.0
1	968.5
2	1903.0
3	23012.5
4	44122.0
5	33501.0
6	22880.0
7	22085.0
8	21290.0
9	21189.5
10	21089.0
11	20598.5
12	20108.0
13	19501.0
14	18894.0
15	18367.5
16	17841.0
17	17003.0
18	16165.0
19	15317.0
20	14469.0
21	13490.5
22	12512.0
23	11856.0
24	11200.0
25	10684.5
26	9946.5
27	9724.0
28	9929.5
29	10135.0
30	10406.5
31	10678.0
32	11334.5
33	11991.0
34	13040.5
35	14090.0
36	14917.5
37	15745.0
38	16564.5
39	17384.0
40	18439.5
41	19495.0
42	20525.5
43	21556.0
44	23144.5
45	24733.0
46	52385.0
47	80037.0
48	57497.5
49	34958.0
50	32635.5
51	30313.0
52	27649.5
53	24986.0
54	24105.5
55	23225.0
56	21657.0
57	20089.0
58	18268.5
59	16448.0
60	14345.5
61	12243.0
62	10415.5
63	8588.0
64	7362.5
65	6137.0
66	5185.5
67	4234.0
68	3595.0
69	2956.0
70	2419.5
71	1883.0
72	1586.0
73	1289.0
74	1150.5
75	818.0
76	624.0
77	481.5
78	339.0
79	229.5
80	120.0
81	96.0
82	72.0
83	54.5
84	37.0
85	29.0
86	21.0
87	14.5
88	8.0
89	6.5
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	657834.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.26650259704098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.97818947168906	46.68892513406552
2	7.741290251574197	8.711506560892952
3	3.5892038174002625	6.058558377391838
4	2.053205971209846	4.621068764453553
5	1.2161943868007874	3.4215502311716586
6	0.7395715046736319	2.4967861193049847
7	0.47089220330287307	1.8546820166047224
8	0.3004161176658185	1.3522691411869396
9	0.1885705693340588	0.9549185786244283
>10	0.6394693709681222	5.915566748692624
>50	0.04340927869553086	1.7244886845177652
>100	0.03494581555712285	3.5853865391251634
>500	0.0016380851042382249	0.5797450213645856
>1k	0.0019110992882779287	2.53050867433461
>5k	0.0	0.0
>10k+	0.0010920567361588165	9.50403940826856
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22558	3.4291325775195567	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTC	15169	2.305900880769313	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCG	13510	2.053709598470131	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGC	10632	1.6162132088034367	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCT	3487	0.5300729363334823	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTC	2737	0.41606241088177265	No Hit
GCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	2417	0.3674179200223765	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAAGCTGT	2386	0.36270548497037247	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCT	2091	0.31786134495936663	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTA	1920	0.29186694515637684	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTT	1435	0.21814013869760457	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGT	878	0.13346832179546816	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	674	0.10245745887260312	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.044084069841327754	0.0
2	0.0	0.0	0.0	0.4695713508271084	0.0
3	0.0	0.0	0.0	0.5546992098310516	0.0
4	0.0	0.0	0.0	1.203343092634312	0.0
5	0.0	0.0	0.0	3.959965584022717	0.0
6	0.0	0.0	0.0	4.310053904176433	0.0
7	0.0	0.0	0.0	4.781297409376833	0.0
8	0.0	0.0	0.0	5.36943970667372	0.0
9	0.0	0.0	0.0	5.492267046093695	0.0
10	0.0	0.0	0.0	8.00673118142267	0.0
11	0.0	0.0	0.0	8.171666408242809	0.0
12	0.0	0.0	0.0	10.225071978645069	0.0
13	0.0	0.0	0.0	10.401256243976444	0.0
14	0.0	0.0	0.0	10.528948032482358	0.0
15	0.0	0.0	0.0	10.927984871563343	0.0
16	0.0	0.0	0.0	11.147341122532433	0.0
17	0.0	0.0	0.0	11.275488953140155	0.0
18	0.0	0.0	0.0	11.365329247196101	0.0
19	0.0	0.0	0.0	11.911363657092823	0.0
20	0.0	0.0	0.0	12.023093972035499	0.0
21	0.0	0.0	0.0	12.10898190120912	0.0
22	4.560421018068388E-4	0.0	0.0	12.278781577115199	0.0
23	4.560421018068388E-4	0.0	0.0	12.372878264121344	0.0
24	4.560421018068388E-4	0.0	0.0	12.455573898582317	0.0
25	4.560421018068388E-4	0.0	0.0	12.513643259545722	0.0
26	4.560421018068388E-4	0.0	0.0	12.605307722008895	0.0
27	4.560421018068388E-4	0.0	0.0	12.719470261494541	0.0
28	4.560421018068388E-4	0.0	0.0	12.80368603629487	0.0
29	4.560421018068388E-4	0.0	0.0	12.894286400520496	0.0
30	4.560421018068388E-4	0.0	0.0	12.995831775189485	0.0
31	4.560421018068388E-4	0.0	0.0	13.091448602534985	0.0
32	4.560421018068388E-4	0.0	0.0	13.210323577072636	0.0
33	4.560421018068388E-4	0.0	0.0	13.321901877981375	0.0
34	4.560421018068388E-4	0.0	0.0	13.451265820860582	0.0
35	4.560421018068388E-4	0.0	0.0	13.576373370789591	0.0
36	4.560421018068388E-4	0.0	0.0	13.700568836514988	0.0
37	6.080561357424517E-4	0.0	0.0	13.828108610986966	0.0
38	6.080561357424517E-4	0.0	0.0	13.967809508173795	0.0
39	6.080561357424517E-4	0.0	0.0	14.15889114883086	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACCG	40	6.8066583E-9	45.000004	14
ATACGTC	30	2.1640808E-6	45.000004	6
GGCGAAC	20	7.031083E-4	45.000004	32
TCGATCA	25	3.8889797E-5	45.0	17
TAGGGTA	35	1.2108649E-7	45.0	5
CGTTTTT	22855	0.0	44.24196	1
CGATGAA	500	0.0	43.649998	19
CCGATGA	500	0.0	42.749996	18
GATACCT	1595	0.0	42.319748	5
ACGGCTG	1475	0.0	41.79661	8
TGATACC	1610	0.0	41.785717	4
GTTTGCG	65	0.0	41.53846	1
CGGCTGT	1490	0.0	41.22483	9
GAATCTG	1845	0.0	41.219513	1
AATCTGT	1870	0.0	40.78877	2
TACGGCT	1510	0.0	40.5298	7
CGGGTAC	50	1.0804797E-9	40.5	6
CGACCTG	140	0.0	40.17857	12
CTACCGA	45	1.9261279E-8	40.0	15
ATACCTG	1700	0.0	39.97059	6
>>END_MODULE