##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933355.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	392846
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.830078453134306	31.0	28.0	31.0	16.0	33.0
2	29.17644318638856	31.0	30.0	33.0	16.0	34.0
3	29.295813117608425	31.0	30.0	33.0	16.0	34.0
4	32.449725846769475	35.0	32.0	37.0	19.0	37.0
5	33.834841133675795	35.0	33.0	37.0	30.0	37.0
6	33.1166793094495	35.0	33.0	37.0	25.0	37.0
7	32.49272488456036	35.0	32.0	37.0	26.0	37.0
8	33.31934905790055	35.0	33.0	37.0	28.0	37.0
9	33.36107278679177	35.0	32.0	38.0	25.0	39.0
10	32.162035505007054	35.0	28.0	39.0	15.0	39.0
11	32.87483899543332	37.0	30.0	39.0	17.0	39.0
12	33.63447254140299	37.0	31.0	39.0	25.0	39.0
13	33.91844132306298	37.0	32.0	39.0	25.0	39.0
14	34.097707498612685	37.0	32.0	40.0	22.0	41.0
15	34.64898458938108	37.0	32.0	40.0	25.0	41.0
16	34.75674946416662	37.0	32.0	40.0	25.0	41.0
17	32.83794158525224	36.0	29.0	40.0	18.0	41.0
18	33.40712900220443	36.0	31.0	39.0	23.0	39.0
19	33.06987470917357	36.0	31.0	37.0	23.0	39.0
20	32.540242232325134	35.0	31.0	36.0	25.0	39.0
21	32.733004790681335	35.0	31.0	37.0	24.0	39.0
22	32.732365863468125	34.0	30.0	37.0	25.0	39.0
23	33.822971342459894	35.0	32.0	38.0	27.0	40.0
24	31.98588250866752	34.0	30.0	37.0	21.0	39.0
25	31.173004179754916	34.0	28.0	36.0	18.0	39.0
26	30.140113428671796	33.0	25.0	36.0	18.0	39.0
27	32.056971434098855	34.0	30.0	37.0	22.0	39.0
28	32.13043279045733	35.0	31.0	37.0	21.0	39.0
29	32.15791938825901	35.0	30.0	38.0	21.0	40.0
30	31.15548586468998	34.0	28.0	37.0	18.0	39.0
31	31.47199666026891	34.0	30.0	37.0	19.0	39.0
32	31.139891967844907	35.0	30.0	37.0	16.0	39.0
33	30.63453108851815	35.0	27.0	38.0	12.0	40.0
34	29.336348085509336	35.0	23.0	38.0	8.0	40.0
35	28.48087036650494	34.0	21.0	37.0	7.0	40.0
36	27.713050406520622	33.0	18.0	37.0	7.0	40.0
37	27.686760206289488	34.0	17.0	37.0	7.0	40.0
38	27.72049098119874	33.0	17.0	38.0	7.0	40.0
39	27.44620029222647	33.0	17.0	37.0	7.0	40.0
40	27.277940974325816	33.0	15.0	37.0	7.0	40.0
41	26.927597582767802	33.0	15.0	37.0	7.0	40.0
42	27.19986457797712	33.0	15.0	37.0	7.0	40.0
43	27.232785875381193	33.0	15.0	38.0	7.0	40.0
44	27.094752142060756	33.0	15.0	38.0	7.0	40.0
45	27.29084424940053	33.0	15.0	38.0	7.0	40.0
46	27.04708460821798	33.0	15.0	38.0	7.0	40.0
47	27.013674569678702	33.0	15.0	38.0	7.0	40.0
48	26.827115460002137	33.0	15.0	37.0	7.0	40.0
49	26.564307642180395	33.0	12.0	37.0	7.0	39.0
50	26.746872820392724	33.0	14.0	37.0	7.0	40.0
51	25.414091017854325	31.0	12.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	13.0
10	23.0
11	27.0
12	34.0
13	55.0
14	103.0
15	205.0
16	395.0
17	841.0
18	1591.0
19	2563.0
20	4036.0
21	5949.0
22	8097.0
23	11443.0
24	15990.0
25	21649.0
26	26106.0
27	24853.0
28	22262.0
29	21345.0
30	22926.0
31	25627.0
32	28568.0
33	32667.0
34	35659.0
35	37586.0
36	26064.0
37	13298.0
38	2829.0
39	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.394622829302065	2.1654796026941856	32.70950957881714	45.7303879891866
2	46.76412640067609	9.553107324498658	30.795273465938305	12.887492808886943
3	10.905545684568507	9.574489749163796	66.37970095151789	13.1402636147498
4	9.686238373306589	2.511416687455135	72.34972482855878	15.452620110679502
5	15.539422572712972	2.8899365145629585	66.82949552750951	14.741145385214564
6	12.102706913141537	8.482204222519767	68.4247771391334	10.990311725205297
7	48.21456754046115	0.9018801260544844	48.507812221583016	2.3757401119013557
8	49.5906792992674	5.861075332318517	41.724492549243216	2.823752819170871
9	46.175600617035684	2.034384975282935	41.67816396246876	10.111850445212628
10	24.819394877381974	13.884321082561616	48.726218416376895	12.570065623679508
11	19.469970420979212	15.468656929178353	52.239045325649236	12.822327324193195
12	14.69939874658263	14.14269204726534	56.16679309449505	14.991116111656986
13	14.006251813687806	14.903040886250594	59.83438802991503	11.256319270146571
14	13.503510281382528	16.22187829327523	55.83511095951085	14.439500465831395
15	11.774843068276118	14.480228893764988	59.93519088905067	13.809737148908225
16	12.996695906283886	14.874530986697076	54.31364962351659	17.81512348350244
17	13.131099718464743	15.721682287715796	56.74920961394542	14.398008379874048
18	14.416845277793334	14.474374182249534	55.298768474160354	15.810012065796775
19	14.06301706012025	17.408348309515688	52.649893342429344	15.87874128793471
20	15.477820825463413	16.68669147706735	56.182829912993896	11.652657784475341
21	15.530004123753328	18.75264098399882	53.298748109946395	12.418606782301461
22	14.392408221033179	14.406663170809935	55.03632466666327	16.164603941493613
23	14.288805282477101	18.53627121060161	53.81014443318756	13.364779073733729
24	14.480228893764988	16.15773101927982	53.8686915483421	15.493348538613095
25	13.438853902037948	19.43433304653732	52.10744159288881	15.019371458535916
26	13.69289747127373	16.198968552562583	54.23397463637151	15.874159339792183
27	14.534194060776997	16.99419110796597	52.421050487977475	16.050564343279554
28	12.058923853112926	17.988982960243963	54.63744062558865	15.314652561054459
29	14.826929636549691	16.23791511177408	51.74852232172403	17.186632929952196
30	13.939813565621135	19.17443476578608	52.8436079277885	14.042143740804287
31	15.027771696797219	18.284518615437094	50.326845634167086	16.36086405359861
32	15.592624081701226	19.874454620894703	49.854141317462826	14.67877997994125
33	16.172495074405745	17.690392672955817	46.50117348782984	19.635938764808603
34	15.956379853683123	20.11322502965539	44.90945561365013	19.02093950301136
35	15.06493638728662	23.204767262489625	43.19911619311384	18.53118015710991
36	18.15138756662916	23.03421697051771	39.109472923232005	19.704922539621123
37	16.891606380108236	25.294644720832082	40.30027033494041	17.51347856411927
38	20.153189799565226	23.721763744571664	39.3920263920213	16.73302006384181
39	19.619647393635166	22.62973277060222	38.044424532768566	19.706195302994047
40	20.90030189947206	21.450644781924723	41.019127087968315	16.629926230634908
41	20.09184260499025	22.82090182921552	37.886601874525894	19.200653691268336
42	18.484597017660864	22.539366571124564	41.575070129261846	17.400966281952723
43	21.055833583643462	21.131944833344363	38.15388218284009	19.65833940017208
44	20.10584300209242	22.648569668521507	37.344404677659945	19.90118265172612
45	18.448705090544387	21.950331682134987	38.00547797355706	21.59548525376356
46	20.467307800003056	23.00494341294044	36.427760496479536	20.09998829057697
47	17.69752014784419	21.425444067140813	41.71736507435483	19.159670710660155
48	18.753659194697157	22.611404978032105	36.93228389750691	21.702651929763825
49	17.767522133355055	21.567993564908384	39.6931622060553	20.97132209568126
50	18.732785875381193	20.62029395742861	38.81393726803887	21.83298289915132
51	18.441068510306838	20.457634798368826	37.32098583159813	23.780310859726203
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	63.0
1	315.0
2	567.0
3	18589.5
4	36612.0
5	25097.0
6	13582.0
7	12938.0
8	12294.0
9	12218.0
10	12142.0
11	11829.0
12	11516.0
13	11014.5
14	10513.0
15	10184.5
16	9856.0
17	9247.5
18	8639.0
19	8016.5
20	7394.0
21	7040.0
22	6686.0
23	6342.5
24	5999.0
25	5632.5
26	5192.0
27	5118.0
28	5220.5
29	5323.0
30	5562.0
31	5801.0
32	6067.0
33	6333.0
34	7002.0
35	7671.0
36	8323.0
37	8975.0
38	9459.0
39	9943.0
40	10575.0
41	11207.0
42	12061.0
43	12915.0
44	13869.5
45	14824.0
46	25133.0
47	35442.0
48	29640.5
49	23839.0
50	22446.5
51	21054.0
52	18799.0
53	16544.0
54	15500.5
55	14457.0
56	13315.0
57	12173.0
58	11278.0
59	10383.0
60	9276.5
61	8170.0
62	7050.5
63	5931.0
64	5236.5
65	4542.0
66	4024.5
67	3507.0
68	2988.0
69	2469.0
70	2058.0
71	1647.0
72	1405.5
73	1164.0
74	1086.0
75	847.5
76	687.0
77	507.0
78	327.0
79	227.0
80	127.0
81	94.5
82	62.0
83	48.0
84	34.0
85	26.0
86	18.0
87	13.5
88	9.0
89	9.0
90	9.0
91	5.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	392846.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.856479801066584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.43790155484156	49.10861167505914
2	8.229088057017062	9.68670310018046
3	3.4868054365031447	6.156632812313894
4	1.8786805115151441	4.422900863145942
5	1.012814747718282	2.9805355370651707
6	0.6356982106900636	2.2448975338232335
7	0.34972857304677896	1.440865488676851
8	0.23044185268083558	1.085039699810397
9	0.1510135800686226	0.7999314952496074
>10	0.5038773781937802	5.064658420467059
>50	0.046590106590734134	1.92026684167417
>100	0.03340422736687343	3.691786083455515
>500	0.0013185879223765826	0.6141335689246299
>1k	0.0017581172298354434	2.7281603276660267
>5k	4.3952930745886086E-4	1.5379208370922177
>10k+	4.3952930745886086E-4	6.516955715395651
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25192	6.4126909781441075	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCG	5945	1.513315650407539	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGC	4097	1.0429023077745478	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTC	4026	1.0248290678790162	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCT	1260	0.32073636997703936	No Hit
GCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	1163	0.296044760542299	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAAGCTGT	999	0.2542981219103669	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTC	783	0.19931474420001732	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTA	592	0.15069518335429152	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCT	487	0.12396715252287155	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCAAAGCTGTCGTATGCCG	482	0.1226943891499468	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTT	453	0.1153123615869832	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCC	434	0.11047586076986911	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	426	0.1084394393731895	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07152930155837148	0.0
2	0.0	0.0	0.0	0.48823202985393765	0.0
3	0.0	0.0	0.0	0.6241631580823019	0.0
4	0.0	0.0	0.0	1.226180233475713	0.0
5	0.0	0.0	0.0	2.6850216115220724	0.0
6	0.0	0.0	0.0	3.1477983739175146	0.0
7	0.0	0.0	0.0	3.5930110017665955	0.0
8	0.0	0.0	0.0	4.163972650860643	0.0
9	0.0	0.0	0.0	4.460017411402942	0.0
10	0.0	0.0	0.0	6.5567677919591905	0.0
11	0.0	0.0	0.0	7.064854930430754	0.0
12	0.0	0.0	0.0	8.635444932619906	0.0
13	0.0	0.0	0.0	8.870906156610987	0.0
14	0.0	0.0	0.0	8.9908004663405	0.0
15	0.0	0.0	0.0	9.312045941666709	0.0
16	0.0	0.0	0.0	9.500669473534158	0.0
17	0.0	0.0	0.0	9.692856742845796	0.0
18	0.0	0.0	0.0	9.887589538903285	0.0
19	0.0	0.0	0.0	10.363093935027976	0.0
20	0.0	0.0	0.0	10.543062675959536	0.0
21	2.545526745849519E-4	0.0	0.0	10.727104259684456	0.0
22	2.545526745849519E-4	0.0	0.0	10.990057172530712	0.0
23	2.545526745849519E-4	0.0	0.0	11.200317681737882	0.0
24	2.545526745849519E-4	0.0	0.0	11.379522764645689	0.0
25	2.545526745849519E-4	0.0	0.0	11.532254369396659	0.0
26	2.545526745849519E-4	0.0	0.0	11.691349791012254	0.0
27	2.545526745849519E-4	0.0	0.0	11.856809029492473	0.0
28	2.545526745849519E-4	0.0	0.0	12.00877697621969	0.0
29	2.545526745849519E-4	0.0	0.0	12.176781741445758	0.0
30	2.545526745849519E-4	0.0	0.0	12.371005432154076	0.0
31	2.545526745849519E-4	0.0	0.0	12.542319382149747	0.0
32	2.545526745849519E-4	0.0	0.0	12.738579494254747	0.0
33	2.545526745849519E-4	0.0	0.0	12.913457181694609	0.0
34	2.545526745849519E-4	0.0	0.0	13.093425922626169	0.0
35	2.545526745849519E-4	0.0	0.0	13.304450089857093	0.0
36	2.545526745849519E-4	0.0	0.0	13.478055013924031	0.0
37	2.545526745849519E-4	0.0	0.0	13.669478625211916	0.0
38	2.545526745849519E-4	0.0	0.0	13.896794163616278	0.0
39	2.545526745849519E-4	0.0	0.0	14.184438685897273	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCT	40	6.7957444E-9	45.0	6
ACGGGCG	20	7.0276106E-4	45.0	5
GAGATCG	20	7.0276106E-4	45.0	20
CGTTTTT	14805	0.0	44.574467	1
TACGGCT	500	0.0	42.3	7
CCGGGAT	80	0.0	42.1875	5
CACCGGT	70	0.0	41.785713	16
CGACCAC	460	0.0	41.576084	12
TGATACC	700	0.0	41.464287	4
TATGGGC	60	3.6379788E-12	41.249996	4
TGCGGGC	170	0.0	41.02941	4
TCGGGAC	55	6.002665E-11	40.909092	5
GCGACCA	470	0.0	40.69149	11
ACGGCTG	520	0.0	40.67308	8
GTTTTTT	16325	0.0	40.658497	2
GAATGAT	2235	0.0	40.57047	1
GATACCT	710	0.0	40.563377	5
ATACGGC	1305	0.0	40.344826	6
AATGATA	2025	0.0	40.333332	2
ATGATAC	2035	0.0	40.245697	3
>>END_MODULE