##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933354.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	568350
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.524250901733087	31.0	28.0	33.0	25.0	34.0
2	28.920065100730184	31.0	28.0	33.0	16.0	34.0
3	28.979019970088853	31.0	28.0	34.0	16.0	34.0
4	33.154619512624265	35.0	32.0	37.0	28.0	37.0
5	34.16874989003255	35.0	33.0	37.0	30.0	37.0
6	33.787199788862495	35.0	33.0	37.0	28.0	37.0
7	33.33350928125275	35.0	32.0	37.0	28.0	37.0
8	33.59069059558371	35.0	33.0	37.0	28.0	37.0
9	34.500543679070994	37.0	34.0	39.0	27.0	39.0
10	34.1728917040556	37.0	32.0	39.0	27.0	39.0
11	34.47050409078913	37.0	33.0	39.0	27.0	39.0
12	34.56794228908243	37.0	33.0	39.0	27.0	39.0
13	34.8007530570951	37.0	34.0	39.0	27.0	39.0
14	35.591385589865396	38.0	34.0	40.0	27.0	41.0
15	35.990384446203926	38.0	34.0	40.0	27.0	41.0
16	35.80386909474795	38.0	34.0	40.0	27.0	41.0
17	35.1937045834433	37.0	33.0	40.0	27.0	41.0
18	34.97163191695258	37.0	33.0	39.0	27.0	40.0
19	34.381073282308435	37.0	32.0	38.0	27.0	40.0
20	34.1187050233131	35.0	32.0	38.0	27.0	40.0
21	34.358441101433975	35.0	33.0	39.0	27.0	40.0
22	34.98999032286443	36.0	33.0	39.0	29.0	40.0
23	35.40475235330342	36.0	34.0	39.0	30.0	40.0
24	34.84657165479018	36.0	33.0	39.0	28.0	40.0
25	33.766847893023666	35.0	33.0	39.0	24.0	40.0
26	33.92359813495205	35.0	33.0	39.0	25.0	40.0
27	34.6311744523621	35.0	33.0	39.0	27.0	40.0
28	34.48740036949063	35.0	33.0	39.0	25.0	40.0
29	34.6337344945896	36.0	33.0	39.0	25.0	40.0
30	33.878397114454124	35.0	33.0	39.0	23.0	40.0
31	33.81566464326559	35.0	33.0	39.0	23.0	40.0
32	33.40187912377936	35.0	33.0	39.0	21.0	40.0
33	33.13490102929533	36.0	33.0	39.0	18.0	41.0
34	32.82290665962875	36.0	32.0	40.0	15.0	41.0
35	32.47046538224686	36.0	32.0	40.0	10.0	41.0
36	31.955754376704494	36.0	31.0	40.0	10.0	41.0
37	31.935742060350137	36.0	31.0	40.0	9.0	41.0
38	31.66143045658485	36.0	30.0	40.0	9.0	41.0
39	31.310519926101875	35.0	29.0	39.0	8.0	40.0
40	31.181094396058768	35.0	29.0	39.0	8.0	40.0
41	30.727224421571215	35.0	26.0	39.0	8.0	40.0
42	30.964280812879387	35.0	27.0	39.0	8.0	40.0
43	30.841048649599717	35.0	27.0	39.0	8.0	40.0
44	30.912504618632884	35.0	27.0	39.0	7.0	40.0
45	31.026469604996922	36.0	28.0	39.0	7.0	40.0
46	30.839992962083222	35.0	27.0	39.0	7.0	40.0
47	30.83909914665259	35.0	27.0	39.0	7.0	40.0
48	30.659890912289963	35.0	27.0	39.0	7.0	40.0
49	30.867812087622063	35.0	27.0	39.0	7.0	40.0
50	30.886983372921616	35.0	27.0	39.0	7.0	40.0
51	29.75648807952846	34.0	24.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	23.0
10	36.0
11	27.0
12	56.0
13	75.0
14	109.0
15	162.0
16	238.0
17	478.0
18	985.0
19	1760.0
20	2822.0
21	4227.0
22	5999.0
23	8467.0
24	12092.0
25	17689.0
26	22587.0
27	24351.0
28	23900.0
29	23285.0
30	24546.0
31	28200.0
32	33705.0
33	39992.0
34	46065.0
35	55031.0
36	62580.0
37	67542.0
38	53434.0
39	7875.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.33694026568136	2.715228292425442	37.877188352247735	35.070643089645465
2	33.299903228644325	13.340547198029384	37.531450690595584	15.828098882730712
3	13.30289434327439	13.440661564177002	56.75270519926102	16.503738893287586
4	12.111375032990235	3.153866455529163	66.06369314682854	18.671065364652062
5	19.58722618105041	3.781472684085511	57.24131257147884	19.389988563385238
6	15.822820445148237	11.532682326031495	60.2908419107944	12.353655318025865
7	60.88501803466174	1.2849476554939738	35.19591800826955	2.6341163015747338
8	60.40520805841471	7.847980997624703	28.744435647048473	3.0023752969121142
9	56.84085510688837	2.9999120260402923	28.036245271399668	12.122987595671681
10	33.40054543855019	20.404856162575875	34.2586434415413	11.93595495733263
11	24.394651183249756	17.736781912553884	42.52291721650391	15.345649687692442
12	20.027975719187122	15.80997624703088	45.83390516407144	18.328142869710565
13	18.74936218879212	16.33975543239201	50.507961643353575	14.402920735462303
14	16.358581859769508	20.69024368786839	45.38541391748043	17.565760534881676
15	14.559162487903581	17.05903052696402	51.88932875868743	16.492478226444973
16	17.362892583795198	18.869886513591975	43.535849388580985	20.231371514031846
17	17.370106448491246	18.514999560130203	46.611946863728335	17.502947127650216
18	17.227060790006156	18.190199700888538	45.755080496173136	18.82765901293217
19	17.158617049353392	20.09465998064573	43.11339843406351	19.633324535937362
20	18.779273335092814	20.722794052960325	46.20568311779713	14.292249494149731
21	18.70379167766341	22.301926629717602	44.2852115773731	14.709070115245886
22	17.21544822732471	16.683381719011173	46.29823172340987	19.802938330254243
23	17.604820972991995	20.978974223629805	46.02498460455705	15.391220198821149
24	19.111463006949943	18.382158880971232	43.63191695258203	18.87446115949679
25	16.675815958476292	24.33799595319785	41.23814550892936	17.748042579396497
26	16.50655405999824	18.01900237529691	44.09589161608164	21.378551948623205
27	18.34274654702208	18.897158441101432	43.12641858010029	19.633676431776195
28	14.97070467141726	19.61467405647928	45.21720770651887	20.197413565584586
29	18.167326471364476	18.195302190551597	42.36474003694906	21.272631301134865
30	17.361660948359287	19.98944312483505	45.986100114366145	16.66279581243952
31	21.425354095187824	18.675640010556876	40.83328934635348	19.065716547901822
32	20.64907187472508	23.093164423330695	39.43379959531978	16.82396410662444
33	18.758863376440573	19.36271663587578	38.03747690683558	23.840943080848067
34	19.115333861177092	21.101961819301486	38.150083575261725	21.632620744259697
35	16.63094923902525	22.95416556699217	41.29937538488607	19.115509809096505
36	20.093780241048652	22.777337907979238	36.86320049265417	20.265681358317938
37	18.097123251517548	25.69490630773291	38.536993050057184	17.670977390692354
38	21.290050145157032	23.30254244743556	34.120700272719276	21.286707134688132
39	20.73053576141462	24.478050497052873	33.71883522477347	21.072578516759037
40	19.608691827219143	22.84296648192135	37.62083223365884	19.927509457200667
41	18.595583707222662	24.734230667722354	35.04917744347673	21.62100818157825
42	18.146916512712238	23.419371865927687	38.59593560306149	19.837776018298584
43	20.010029031406702	22.04451482361221	35.41426937626462	22.53118676871646
44	20.687428521157738	22.383918360165392	34.09008533474092	22.838567783935957
45	19.028415588985663	22.102401689100027	34.11964458520278	24.749538136711532
46	22.158353127474268	24.198117357262248	33.21720770651887	20.42632180874461
47	16.683205771091757	22.36438814111023	40.32040116125627	20.632004926541743
48	19.109703527755784	24.057534969648984	33.859417612386736	22.973343890208497
49	18.128793877012402	22.35717427641418	38.71206122987596	20.801970616697457
50	18.807073106360516	21.477082783496083	37.20577109175684	22.51007301838656
51	18.23717779537257	21.917480425793965	34.71082959444004	25.134512184393422
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	37.0
1	771.0
2	1505.0
3	17097.0
4	32689.0
5	23937.5
6	15186.0
7	14180.0
8	13174.0
9	12750.5
10	12327.0
11	11656.5
12	10986.0
13	10166.0
14	9346.0
15	8605.0
16	7864.0
17	7362.5
18	6861.0
19	6338.5
20	5816.0
21	5512.5
22	5209.0
23	5094.5
24	4980.0
25	5286.5
26	6007.5
27	6422.0
28	6996.0
29	7570.0
30	8567.0
31	9564.0
32	10249.5
33	10935.0
34	12340.0
35	13745.0
36	14574.5
37	15404.0
38	16266.0
39	17128.0
40	18112.0
41	19096.0
42	20129.5
43	21163.0
44	22875.5
45	24588.0
46	48274.0
47	71960.0
48	53226.5
49	34493.0
50	32716.0
51	30939.0
52	28516.5
53	26094.0
54	25544.0
55	24994.0
56	24323.5
57	23653.0
58	22631.0
59	21609.0
60	19448.5
61	17288.0
62	15209.5
63	13131.0
64	11461.0
65	9791.0
66	7965.0
67	6139.0
68	5069.5
69	4000.0
70	3322.0
71	2644.0
72	2230.5
73	1817.0
74	1491.0
75	971.5
76	778.0
77	549.5
78	321.0
79	256.0
80	191.0
81	138.5
82	86.0
83	56.5
84	27.0
85	25.0
86	23.0
87	16.0
88	9.0
89	6.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	2.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	568350.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.027999702958525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.00914425752865	38.526134460407334
2	8.382751573501348	8.387445864582013
3	4.76236342242749	7.14754547647749
4	3.238546020800906	6.4807191746658095
5	2.166789604758656	5.4200074851619835
6	1.4784892313266023	4.437951529538078
7	0.9637297048878168	3.374942857290201
8	0.6281624996377035	2.514057067622778
9	0.39790308992030043	1.7915666097905167
>10	0.8994482492998884	6.885760407859041
>50	0.04116906320864735	1.423098111650679
>100	0.0268493886665334	2.380725269473853
>500	7.159836977742232E-4	0.22906399004416286
>1k	0.0025059429422097813	2.5120027555585835
>5k	3.579918488871116E-4	1.4890054833676074
>10k+	0.0010739755466613347	6.999973456509855
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15148	2.66525908331134	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	13660	2.4034485792205507	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	10277	1.8082167678367203	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	8314	1.462831002023401	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	3272	0.5757015923286707	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC	2299	0.4045042667370458	No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	2083	0.3664995161432216	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGACCTTT	1808	0.31811383830386203	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT	1639	0.28837863992258295	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTA	1634	0.2874989003255036	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT	1291	0.2271487639658661	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGT	754	0.13266473123955308	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05049705287234978	0.0
2	0.0	0.0	0.0	0.48332893463534793	0.0
3	0.0	0.0	0.0	0.5827395091053048	0.0
4	0.0	0.0	0.0	1.2902260930764493	0.0
5	0.0	0.0	0.0	4.187912377936131	0.0
6	0.0	0.0	0.0	4.579748394475235	0.0
7	0.0	0.0	0.0	5.042315474619513	0.0
8	0.0	0.0	0.0	5.640010556875165	0.0
9	0.0	0.0	0.0	5.796076361397026	0.0
10	0.0	0.0	0.0	8.07037916776634	0.0
11	0.0	0.0	0.0	8.261282660332542	0.0
12	0.0	0.0	0.0	10.149203835664643	0.0
13	0.0	0.0	0.0	10.329550453065892	0.0
14	0.0	0.0	0.0	10.467845517726753	0.0
15	0.0	0.0	0.0	10.874637107416206	0.0
16	0.0	0.0	0.0	11.09122899621712	0.0
17	0.0	0.0	0.0	11.206122987595672	0.0
18	0.0	0.0	0.0	11.296736166094837	0.0
19	0.0	0.0	0.0	11.795548517638778	0.0
20	0.0	0.0	0.0	11.920471540424035	0.0
21	0.0	0.0	0.0	12.019178323216329	0.0
22	0.0	0.0	0.0	12.21553620128442	0.0
23	0.0	0.0	0.0	12.33430104689012	0.0
24	0.0	0.0	0.0	12.43529515263482	0.0
25	0.0	0.0	0.0	12.525908331133984	0.0
26	0.0	0.0	0.0	12.63939473915721	0.0
27	0.0	0.0	0.0	12.806193366763438	0.0
28	0.0	0.0	0.0	12.914049441365355	0.0
29	0.0	0.0	0.0	13.039324359989443	0.0
30	0.0	0.0	0.0	13.192574997800651	0.0
31	0.0	0.0	0.0	13.31820181226357	0.0
32	0.0	0.0	0.0	13.465294272895223	0.0
33	3.5189583883170583E-4	0.0	0.0	13.599014691651272	0.0
34	3.5189583883170583E-4	0.0	0.0	13.76792469429049	0.0
35	3.5189583883170583E-4	0.0	0.0	13.94827131169174	0.0
36	3.5189583883170583E-4	0.0	0.0	14.119820533122196	0.0
37	3.5189583883170583E-4	0.0	0.0	14.295944400457465	0.0
38	3.5189583883170583E-4	0.0	0.0	14.50655405999824	0.0
39	3.5189583883170583E-4	0.0	0.0	14.834872877628221	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGCG	40	6.8048394E-9	45.000004	1
TCCGTCT	20	7.0302724E-4	45.000004	7
CCCGATC	40	6.8048394E-9	45.000004	41
CGTTTTT	11460	0.0	44.05759	1
CCGATGA	310	0.0	42.82258	18
CACGACC	90	0.0	42.5	27
CGATGAA	315	0.0	42.142857	19
TGATACC	1195	0.0	41.799164	4
GATACCT	1185	0.0	41.772156	5
TACGGCT	920	0.0	40.84239	7
TTAGCGG	50	1.0786607E-9	40.5	2
TACCGGG	50	1.0786607E-9	40.5	3
ATACCTG	1230	0.0	40.2439	6
ACGGCTG	935	0.0	40.18717	8
AGGCACG	45	1.9254003E-8	40.0	10
GAATCTG	1720	0.0	39.898254	1
CGTTCAT	40	3.4541154E-7	39.375004	17
ATGATAC	3780	0.0	39.166668	3
GAATGAT	3970	0.0	38.992447	1
AATGATC	295	0.0	38.898304	2
>>END_MODULE