##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933352.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	698777
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.569912862043257	31.0	28.0	33.0	25.0	34.0
2	28.99443456209921	31.0	28.0	33.0	16.0	34.0
3	28.981337393760814	31.0	28.0	34.0	16.0	34.0
4	33.227171186229654	35.0	32.0	37.0	28.0	37.0
5	34.238244819162624	35.0	33.0	37.0	30.0	37.0
6	33.82434882659275	35.0	33.0	37.0	28.0	37.0
7	33.50170225980535	35.0	32.0	37.0	28.0	37.0
8	33.66120951319233	35.0	33.0	37.0	28.0	37.0
9	34.70741452566413	37.0	34.0	39.0	28.0	39.0
10	34.3709667032544	37.0	32.0	39.0	27.0	39.0
11	34.596884270661455	37.0	33.0	39.0	27.0	39.0
12	34.74978855915406	37.0	33.0	39.0	27.0	39.0
13	34.903757565002856	37.0	34.0	39.0	27.0	39.0
14	35.734380209995464	38.0	34.0	40.0	27.0	41.0
15	36.08920728644475	38.0	34.0	40.0	27.0	41.0
16	35.9160547642524	38.0	34.0	40.0	27.0	41.0
17	35.30941630877948	37.0	33.0	40.0	27.0	41.0
18	35.06383724707596	37.0	33.0	39.0	27.0	40.0
19	34.350599690602294	37.0	33.0	38.0	27.0	40.0
20	33.9274961826162	35.0	32.0	38.0	27.0	40.0
21	34.23566459685994	35.0	33.0	38.0	27.0	40.0
22	34.77361876535719	35.0	33.0	39.0	29.0	40.0
23	35.18533094248952	35.0	34.0	39.0	30.0	40.0
24	34.6097424500234	35.0	33.0	39.0	27.0	40.0
25	33.59400352329856	35.0	33.0	38.0	24.0	40.0
26	33.630525904544655	35.0	32.0	38.0	24.0	40.0
27	34.310289262525814	35.0	33.0	39.0	26.0	40.0
28	34.17663288860395	35.0	33.0	39.0	25.0	40.0
29	34.18453812875924	35.0	33.0	39.0	25.0	40.0
30	33.48315413930338	35.0	33.0	39.0	22.0	40.0
31	33.382604178443195	35.0	33.0	39.0	22.0	40.0
32	32.822009024338236	35.0	32.0	39.0	18.0	40.0
33	32.33686855749402	35.0	31.0	39.0	15.0	40.0
34	31.793586509000725	35.0	31.0	39.0	10.0	40.0
35	31.32083053678069	35.0	29.0	39.0	8.0	41.0
36	30.810202682687038	35.0	27.0	39.0	7.0	40.0
37	30.743766609376095	35.0	26.0	40.0	7.0	40.0
38	30.391639965253578	35.0	24.0	39.0	7.0	40.0
39	30.04612344138402	35.0	23.0	39.0	7.0	40.0
40	29.72087089300306	35.0	23.0	39.0	7.0	40.0
41	29.26820144337893	35.0	21.0	39.0	7.0	40.0
42	29.4645759662954	35.0	22.0	39.0	7.0	40.0
43	29.353036805733446	35.0	21.0	39.0	7.0	40.0
44	29.443217220944593	35.0	22.0	39.0	7.0	40.0
45	29.504425589279556	35.0	22.0	39.0	7.0	40.0
46	29.348340028363843	35.0	22.0	39.0	7.0	40.0
47	29.22146407222905	35.0	22.0	38.0	7.0	40.0
48	29.089304599321387	35.0	22.0	38.0	7.0	40.0
49	29.235302535715974	35.0	22.0	38.0	7.0	40.0
50	29.26781648508752	35.0	22.0	39.0	7.0	40.0
51	28.03761142682143	33.0	20.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	27.0
10	58.0
11	53.0
12	68.0
13	99.0
14	117.0
15	190.0
16	385.0
17	727.0
18	1356.0
19	2582.0
20	4087.0
21	5976.0
22	8792.0
23	12724.0
24	18394.0
25	26318.0
26	33378.0
27	35684.0
28	33462.0
29	31301.0
30	32807.0
31	36015.0
32	41571.0
33	48380.0
34	54558.0
35	64148.0
36	70862.0
37	72368.0
38	54472.0
39	7805.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.74752317262875	2.658358818335463	33.976218450235194	38.61789955880059
2	37.86844730149962	13.483987023041685	34.38593428232469	14.261631393134003
3	12.865907149204968	13.795102013947226	58.40361088015204	14.93537995669577
4	11.49909055392493	3.0175578188749776	68.3497024086368	17.133649218563292
5	19.24405067711158	3.491242556638241	59.65279338043467	17.6119133858155
6	14.908762022791247	11.835535514191223	61.965834593869005	11.289867869148527
7	55.79147567822066	1.2208472803197585	40.46899082253709	2.518686218922489
8	55.1645231597491	8.182581853724436	33.662670637413655	2.990224349112807
9	52.126930336859964	2.5256984703274434	33.11657367085637	12.230797521956218
10	32.3955997406898	16.35729281301474	39.202206140156306	12.044901306139156
11	25.170547971670505	16.03759139181742	44.77937882901126	14.012481807500821
12	19.323761371653617	14.671776546738085	48.903727512496836	17.100734569111463
13	18.379969575415334	15.110829348991167	53.594065059382324	12.915136016211182
14	16.1339025182569	19.209991170287516	48.755039161277494	15.901067150178097
15	13.705373817398112	15.026253010617122	55.381616738959636	15.886756433025129
16	16.770300110049416	16.792052400121925	46.42897519523396	20.0086722945947
17	16.778743433169666	17.177583120222902	49.3509374235271	16.69273602308032
18	17.476390894376888	15.809621667570628	48.59833680845248	18.1156506296
19	16.90482085128732	18.000163142175545	45.930246702453	19.164769304084135
20	18.547548073276594	18.81501537686558	49.57490014697106	13.062536402886757
21	18.398573507714193	20.58553730303087	47.32969173284181	13.686197456413133
22	16.660536909486147	14.248751747696332	50.08407546327369	19.006635879543833
23	16.888077312218346	19.498352120919833	49.05227275654465	14.56129781031717
24	18.229850152480694	17.286487677756995	46.72849850524559	17.755163664516722
25	16.369456922594765	22.977287460806522	44.36293695985987	16.290318656738844
26	16.41525121748426	16.21060796219681	47.45648468681711	19.91765613350182
27	18.32143874225969	17.323552435183185	45.53341051580118	18.821598306755945
28	14.287390684009349	19.13943933472338	47.84487755034868	18.728292430918593
29	17.633808783059546	19.292850222603207	44.455670407011105	18.617670587326142
30	17.22795684460135	18.620389623585208	46.72263111121288	17.429022420600564
31	21.939760467216292	16.86031452094159	42.99969804386807	18.200226967974046
32	20.106414492749476	19.923237313191475	41.836093632160186	18.13425456189886
33	19.182943914868407	17.77448313267323	41.90793343226809	21.134639520190277
34	21.75787125220206	22.41616424123862	39.90314506630871	15.92281944025061
35	17.306808896114212	25.165682327838496	37.22732717304662	20.300181603000674
36	21.572833679414178	26.664586842440436	34.73826413863078	17.024315339514608
37	18.990750983504036	23.411474619227594	36.29641502224601	21.30135937502236
38	22.611219316033583	23.113668595274316	35.72699158672938	18.548120501962714
39	23.75693533130026	22.30253714704405	33.460889525556794	20.479637996098898
40	21.4659326222815	23.710711714896167	37.28786150660368	17.53549415621865
41	22.044514916776027	25.833420390196014	33.045306299434586	19.07675839359338
42	19.503503979094905	24.5453127392573	37.295016865180166	18.656166416467627
43	21.697050704301944	23.601377835847487	34.10615976198415	20.595411697866414
44	22.507180402331503	21.82713512322243	33.90523729315647	21.760447181289596
45	20.441571488472	21.845166626835173	33.540886434441894	24.17237545025094
46	23.024799041754378	23.292838774029484	32.74177598862012	20.94058619559602
47	17.43145524251657	22.179894301043106	40.37954884033104	20.009101616109287
48	20.29588838785478	24.58309303254114	33.22118501324457	21.89983356635951
49	19.402470315994947	22.32614983034645	37.80118693088067	20.470192922777937
50	19.585075066866825	21.955216041741497	36.20682993286843	22.252878958523247
51	18.912185146334238	21.788353079737885	34.24883761199925	25.050624161928624
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78.0
1	1092.0
2	2106.0
3	24038.0
4	45970.0
5	33590.0
6	21210.0
7	20030.5
8	18851.0
9	18163.0
10	17475.0
11	16625.0
12	15775.0
13	14782.0
14	13789.0
15	12752.5
16	11716.0
17	11011.5
18	10307.0
19	9658.5
20	9010.0
21	8538.0
22	8066.0
23	7807.0
24	7548.0
25	7644.5
26	8106.5
27	8472.0
28	9153.0
29	9834.0
30	10283.0
31	10732.0
32	11251.5
33	11771.0
34	13055.5
35	14340.0
36	14797.5
37	15255.0
38	15759.5
39	16264.0
40	17177.0
41	18090.0
42	19399.5
43	20709.0
44	22760.0
45	24811.0
46	54936.0
47	85061.0
48	61469.5
49	37878.0
50	36178.0
51	34478.0
52	32183.5
53	29889.0
54	30510.0
55	31131.0
56	30442.0
57	29753.0
58	28745.5
59	27738.0
60	24617.5
61	21497.0
62	19247.0
63	16997.0
64	15188.0
65	13379.0
66	11577.0
67	9775.0
68	8485.5
69	7196.0
70	6108.5
71	5021.0
72	4357.0
73	3693.0
74	3055.5
75	1951.0
76	1484.0
77	1129.0
78	774.0
79	568.5
80	363.0
81	257.0
82	151.0
83	119.0
84	87.0
85	62.5
86	38.0
87	32.0
88	26.0
89	21.5
90	17.0
91	12.5
92	8.0
93	5.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	698777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.89812465359542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3246635907883	36.408532975945235
2	6.686312386139846	6.137783987438515
3	3.508953776967222	4.83163193576838
4	2.4734227195750655	4.5410185721636545
5	1.873671919620231	4.299901366333539
6	1.4816314194087197	4.080246214722296
7	1.0865416194740063	3.490915588435616
8	0.8517895107793901	3.1276432915502
9	0.6422495837483738	2.653024630824237
>10	1.9701824821111598	13.067132396525324
>50	0.05851410313760669	1.9159839670413052
>100	0.0360573327857856	2.864829709260063
>500	0.002530339142861967	0.7608479024136684
>1k	0.002214046750004221	2.5651941300609655
>5k	0.0	0.0
>10k+	0.0012651695714309835	9.25531333151708
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22654	3.2419498638335265	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTC	16396	2.346385184400746	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCG	12691	1.816173113883256	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGC	12013	1.7191464515861283	No Hit
GAACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCT	4271	0.6112107296032926	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTC	2810	0.4021311519984201	No Hit
GCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGC	2412	0.34517449772960473	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGCTGAAT	2341	0.33501388855099695	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCTGAATCGTA	2268	0.32456706502932986	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCT	2055	0.2940852374935065	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTT	1513	0.21652115052441623	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACGCTGAATCGT	927	0.1326603480080197	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	859	0.12292906034400103	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.051089260236098215	0.0
2	0.0	0.0	0.0	0.4513600190046324	0.0
3	0.0	0.0	0.0	0.5290672131452524	0.0
4	0.0	0.0	0.0	1.269646825811382	0.0
5	0.0	0.0	0.0	4.026034056644681	0.0
6	0.0	0.0	0.0	4.362908338425563	0.0
7	0.0	0.0	0.0	4.813123500057959	0.0
8	0.0	0.0	0.0	5.384979757490587	0.0
9	0.0	0.0	0.0	5.497032672798332	0.0
10	0.0	0.0	0.0	7.7654244487153985	0.0
11	0.0	0.0	0.0	7.933432268091251	0.0
12	0.0	0.0	0.0	10.082472662952558	0.0
13	0.0	0.0	0.0	10.2692275217988	0.0
14	0.0	0.0	0.0	10.412907122014605	0.0
15	0.0	0.0	0.0	10.84451835134814	0.0
16	0.0	0.0	0.0	11.05989464450032	0.0
17	0.0	0.0	0.0	11.163218022344754	0.0
18	0.0	0.0	0.0	11.241354537999962	0.0
19	0.0	0.0	0.0	11.740369245123981	0.0
20	0.0	0.0	0.0	11.856715375577616	0.0
21	0.0	0.0	0.0	11.945584929097551	0.0
22	0.0	0.0	0.0	12.125327536538839	0.0
23	0.0	0.0	0.0	12.22507323509503	0.0
24	0.0	0.0	0.0	12.315660074673323	0.0
25	0.0	0.0	0.0	12.386211910237458	0.0
26	0.0	0.0	0.0	12.484812751421412	0.0
27	0.0	0.0	0.0	12.63207003092546	0.0
28	0.0	0.0	0.0	12.70963411789455	0.0
29	0.0	0.0	0.0	12.818538675428641	0.0
30	0.0	0.0	0.0	12.955635345754082	0.0
31	0.0	0.0	0.0	13.050658507649795	0.0
32	0.0	0.0	0.0	13.170152995877082	0.0
33	0.0	0.0	0.0	13.27619540998058	0.0
34	0.0	0.0	0.0	13.430751155232642	0.0
35	0.0	0.0	0.0	13.590744973002833	0.0
36	0.0	0.0	0.0	13.730560679587336	0.0
37	0.0	0.0	0.0	13.862505491737707	0.0
38	0.0	0.0	0.0	14.037811776861574	0.0
39	0.0	0.0	0.0	14.395007276999673	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	25	3.8892293E-5	45.000004	32
CGTTTTT	17560	0.0	44.24402	1
CCGATGA	385	0.0	43.246754	18
ACGGCTG	1410	0.0	42.60638	8
TACGGCT	1435	0.0	42.1777	7
GATACCT	1545	0.0	41.359222	5
CGGCTGT	1480	0.0	40.743244	9
TGATACC	1565	0.0	40.6869	4
AATCTGT	1865	0.0	40.294907	2
GAATCTG	1870	0.0	40.187164	1
CACCGGT	90	0.0	40.0	16
CGATGAA	400	0.0	39.937504	19
ATACCTG	1600	0.0	39.937504	6
ATGATAC	4910	0.0	39.82179	3
AATGATA	4870	0.0	39.686855	2
CGACCAC	1180	0.0	39.661015	12
ACCACCG	715	0.0	39.65035	14
GAATGAT	5190	0.0	39.62428	1
ATACGGC	3440	0.0	39.309597	6
TTGCTCG	75	0.0	39.0	1
>>END_MODULE