##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933351.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	531381
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.819666115273222	31.0	28.0	31.0	16.0	33.0
2	29.31763837999477	31.0	30.0	33.0	16.0	34.0
3	29.504976655168324	31.0	30.0	33.0	16.0	34.0
4	32.227761248520366	35.0	32.0	37.0	19.0	37.0
5	33.56764167330032	35.0	33.0	37.0	28.0	37.0
6	33.101889228256184	35.0	33.0	37.0	26.0	37.0
7	31.753284366584428	35.0	30.0	35.0	23.0	37.0
8	33.17204040039068	35.0	33.0	35.0	28.0	37.0
9	32.76514591225505	35.0	31.0	37.0	25.0	39.0
10	30.789627404818766	35.0	27.0	39.0	15.0	39.0
11	31.629087227431917	35.0	27.0	39.0	15.0	39.0
12	32.66792753222264	35.0	30.0	39.0	21.0	39.0
13	32.81583647138306	35.0	31.0	39.0	21.0	39.0
14	33.226824067853386	36.0	31.0	40.0	18.0	41.0
15	33.842611986503094	36.0	31.0	40.0	23.0	41.0
16	33.99742369411025	37.0	32.0	40.0	23.0	41.0
17	32.0202303055623	35.0	27.0	39.0	17.0	41.0
18	32.78017467692673	36.0	30.0	39.0	21.0	39.0
19	32.56289931329874	36.0	30.0	37.0	19.0	39.0
20	32.385890726239744	34.0	30.0	36.0	24.0	39.0
21	32.67077294822359	35.0	31.0	37.0	23.0	39.0
22	32.91504212608279	35.0	30.0	37.0	25.0	39.0
23	34.04112303601371	35.0	33.0	38.0	27.0	40.0
24	32.162457822165265	34.0	30.0	37.0	21.0	39.0
25	31.33536577333401	34.0	29.0	37.0	18.0	39.0
26	30.625983992653104	33.0	25.0	37.0	18.0	39.0
27	32.21910644151748	34.0	30.0	37.0	22.0	39.0
28	32.36896125378966	35.0	31.0	37.0	21.0	39.0
29	32.624316639096996	35.0	31.0	38.0	22.0	40.0
30	31.575459039747376	35.0	30.0	38.0	18.0	40.0
31	31.84865473172733	35.0	30.0	37.0	20.0	40.0
32	31.668307673778326	35.0	30.0	37.0	18.0	40.0
33	31.738169035023834	35.0	30.0	38.0	16.0	40.0
34	30.947903669871522	35.0	29.0	38.0	12.0	40.0
35	30.380741878238027	35.0	27.0	38.0	10.0	40.0
36	29.78073359792691	34.0	25.0	38.0	8.0	40.0
37	29.927912364198193	35.0	25.0	38.0	8.0	40.0
38	29.819299146939766	35.0	24.0	38.0	8.0	40.0
39	29.3831695149055	34.0	23.0	38.0	7.0	40.0
40	29.112967908148768	34.0	23.0	38.0	8.0	40.0
41	28.656365206885454	33.0	22.0	37.0	7.0	40.0
42	29.027377719564683	34.0	23.0	38.0	7.0	40.0
43	29.020239715006745	34.0	23.0	38.0	7.0	40.0
44	29.040855807791395	34.0	23.0	38.0	7.0	40.0
45	29.221515259296062	34.0	23.0	38.0	7.0	40.0
46	29.038207990123848	34.0	23.0	38.0	7.0	40.0
47	29.035959132900874	34.0	23.0	38.0	7.0	40.0
48	28.767069202700135	34.0	23.0	37.0	7.0	40.0
49	28.53908400940192	33.0	23.0	37.0	7.0	39.0
50	28.74116688402483	34.0	23.0	37.0	7.0	39.0
51	27.164209860721403	32.0	20.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	15.0
10	35.0
11	45.0
12	57.0
13	104.0
14	158.0
15	275.0
16	597.0
17	1074.0
18	1840.0
19	2984.0
20	4483.0
21	6411.0
22	8668.0
23	11935.0
24	16393.0
25	22342.0
26	27742.0
27	29728.0
28	29760.0
29	30736.0
30	33702.0
31	38219.0
32	43154.0
33	48141.0
34	53165.0
35	56245.0
36	39538.0
37	19798.0
38	3960.0
39	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.757386884363573	2.6653192342217733	37.267610245755876	40.309683635658786
2	40.493355991275564	8.178689113837342	37.435851112478616	13.89210378240848
3	13.761124315698153	8.25885758053073	63.04628882101543	14.933729282755687
4	12.27029193742343	3.122618234374206	67.01707437789459	17.59001545030778
5	16.377326249903554	3.5328700122887344	62.78056610981575	17.30923762799197
6	15.529723494065465	7.733057824799908	64.32503232144168	12.412186359692951
7	57.26136237464268	1.2695222448676184	38.819980390717774	2.649134989771934
8	58.70364201956788	4.947297701649099	33.407667944469225	2.9413923343137975
9	54.89883906274405	2.4737429452690254	33.71705047790569	8.910367514081234
10	31.61215775498183	16.997032261221236	39.485416302050695	11.905393681746244
11	23.672844907890948	16.660174149997836	45.95911408198637	13.707866860124845
12	19.334714639778237	15.058874893908513	49.72195091657398	15.884459549739264
13	17.61391543920464	15.545719549626352	53.15489262882941	13.685472382339603
14	15.959546916430961	16.26102551653145	51.089519572585395	16.68990799445219
15	14.225762682519699	15.259484249530939	54.56009906263114	15.954654005318217
16	16.85626697228542	15.231632293966099	48.57663333841443	19.335467395334042
17	16.93380079453349	15.922661894196445	50.00291692777875	17.140620383491317
18	17.430054894698905	14.987927682773753	49.67076353877914	17.911253883748195
19	17.331443916888258	17.419516316917615	47.26138119353157	17.98765857266255
20	18.485794561717487	18.22948129496538	49.515695894283006	13.769028249034122
21	18.759797584031045	18.511200061725955	47.77212583814626	14.956876516096736
22	17.35797855023044	15.218082693961582	49.474106149824706	17.949832605983275
23	17.238102227968255	18.437053639479018	48.6607537717758	15.66409036077692
24	17.222858927963173	16.949796850094376	48.13589496048974	17.691449261452703
25	16.770264650034534	19.24777137308259	46.01444161533815	17.96752236154473
26	16.603152916645495	17.123118816818817	47.57509207141392	18.698636195121768
27	16.751822138917273	17.210250272403414	47.26025206019786	18.777675528481446
28	14.97117887165706	18.367423750566918	48.35043029389459	18.310967083881433
29	17.337842339112612	17.964699528210456	46.11324078203774	18.584217350639186
30	17.35703760578568	17.570255616967863	47.646227471437626	17.426479305808826
31	19.850164006616723	17.54089815029141	45.05844205946393	17.55049578362794
32	19.282774506427593	19.0917627841417	44.015875614671955	17.60958709475875
33	18.650459839550155	18.303063150545466	43.379044414459685	19.6674325954447
34	19.753999484362446	21.78117019614928	41.644883802770515	16.81994651671776
35	17.151158961272607	24.057502996908056	39.17471644639157	19.616621595427763
36	20.717902973572635	24.003680974667894	37.507174701391286	17.771241350368193
37	18.90451483963484	23.243586052192306	37.889009957074116	19.962889151098743
38	21.361697162676123	22.071545651801628	38.80831267960277	17.75844450591948
39	21.708717473902905	21.99965749622211	36.722803412240935	19.568821617634054
40	20.86977140695659	23.117499496594725	38.85385439072906	17.158874705719626
41	20.670479373556827	24.299325719210888	36.633225501099965	18.396969406132325
42	18.855585728507418	23.9056345635241	38.50796321283599	18.730816495132494
43	20.148255206716083	22.7134579520156	37.272691345757565	19.865595495510753
44	20.95351546254006	21.781546573927184	36.78133015670489	20.48360780682787
45	19.680041251004457	21.917230762861298	36.21826900096165	22.184458985172597
46	20.79148482915272	22.636488696434384	36.1958745231764	20.376151951236494
47	17.651553216994962	21.923817373974604	40.54905990240524	19.87556950662519
48	19.56505783985502	23.260711241086902	36.34924846767198	20.824982451386106
49	18.803081028489917	22.27083768520139	38.551058468405905	20.375022817902785
50	18.91768806186145	21.94621185175985	37.89653751263218	21.23956257374652
51	18.645755117326363	21.359815273786605	37.10708512348014	22.887344485406892
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	119.0
1	615.5
2	1112.0
3	25887.5
4	50663.0
5	33464.0
6	16265.0
7	15343.5
8	14422.0
9	13992.5
10	13563.0
11	12714.0
12	11865.0
13	11105.5
14	10346.0
15	9361.0
16	8376.0
17	7637.5
18	6899.0
19	6546.0
20	6193.0
21	5818.5
22	5444.0
23	5209.0
24	4974.0
25	5021.0
26	5364.0
27	5660.0
28	6195.5
29	6731.0
30	7207.0
31	7683.0
32	8088.5
33	8494.0
34	9474.5
35	10455.0
36	11014.5
37	11574.0
38	12071.5
39	12569.0
40	13457.0
41	14345.0
42	15383.5
43	16422.0
44	17781.5
45	19141.0
46	30715.0
47	42289.0
48	36814.5
49	31340.0
50	30216.5
51	29093.0
52	26732.0
53	24371.0
54	23985.0
55	23599.0
56	22534.0
57	21469.0
58	20792.5
59	20116.0
60	18399.0
61	16682.0
62	15137.5
63	13593.0
64	12442.0
65	11291.0
66	10005.5
67	8720.0
68	7834.0
69	6948.0
70	5841.5
71	4735.0
72	4118.5
73	3502.0
74	2908.5
75	1880.0
76	1445.0
77	1113.5
78	782.0
79	569.5
80	357.0
81	266.0
82	175.0
83	131.5
84	88.0
85	60.5
86	33.0
87	31.0
88	29.0
89	21.5
90	14.0
91	11.5
92	9.0
93	5.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	531381.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.65577586588833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.09830703258062	36.43737108417411
2	7.56695896688746	7.0608468309095045
3	3.889263865774802	5.443698696146627
4	2.5633533808701365	4.783809632117767
5	1.888003016393754	4.404312278349403
6	1.4186641185984432	3.971332508778419
7	1.0926188701973432	3.5683886780337155
8	0.8026361393314368	2.9958089454807544
9	0.6042194881135283	2.5371296110103896
>10	1.9833701697536936	13.13595882726008
>50	0.053093505533812334	1.6804081930687893
>100	0.03498409278152632	3.0470480174210284
>500	0.0016463102485424147	0.5052169986031463
>1k	0.0020578878106780186	2.556424896390607
>5k	4.115775621356037E-4	1.0286763441721987
>10k+	4.115775621356037E-4	6.843568458083439
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35639	6.706863813346732	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCG	5357	1.0081278781138203	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGC	4750	0.893897222520188	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTC	4660	0.8769602225145423	No Hit
GAACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCT	1717	0.323120322329929	No Hit
GCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGC	1114	0.209642422292103	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGCTGAAT	1072	0.20173848895613505	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTC	853	0.1605251222757306	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCTGAATCGTA	723	0.1360605667120202	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTT	536	0.10086924447806753	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8818888895161853E-4	0.0	0.0	0.05702123335234041	0.0
2	1.8818888895161853E-4	0.0	0.0	0.3586880223417849	0.0
3	1.8818888895161853E-4	0.0	0.0	0.4661438779331591	0.0
4	1.8818888895161853E-4	0.0	0.0	1.0164081892276917	0.0
5	1.8818888895161853E-4	0.0	0.0	2.2004926785112753	0.0
6	1.8818888895161853E-4	0.0	0.0	2.544501967514834	0.0
7	1.8818888895161853E-4	0.0	0.0	2.8949096787427475	0.0
8	1.8818888895161853E-4	0.0	0.0	3.341293723335987	0.0
9	1.8818888895161853E-4	0.0	0.0	3.587821167862607	0.0
10	1.8818888895161853E-4	0.0	0.0	5.154870046162735	0.0
11	1.8818888895161853E-4	0.0	0.0	5.606335190757667	0.0
12	1.8818888895161853E-4	0.0	0.0	6.969010935656337	0.0
13	1.8818888895161853E-4	0.0	0.0	7.148354946827229	0.0
14	1.8818888895161853E-4	0.0	0.0	7.266725757977797	0.0
15	1.8818888895161853E-4	0.0	0.0	7.536588624734418	0.0
16	1.8818888895161853E-4	0.0	0.0	7.694102724786923	0.0
17	1.8818888895161853E-4	0.0	0.0	7.829410535943137	0.0
18	1.8818888895161853E-4	0.0	0.0	7.973375035991125	0.0
19	1.8818888895161853E-4	0.0	0.0	8.327734713887022	0.0
20	1.8818888895161853E-4	0.0	0.0	8.48317873616106	0.0
21	1.8818888895161853E-4	0.0	0.0	8.648784958438483	0.0
22	1.8818888895161853E-4	0.0	0.0	8.887596658518088	0.0
23	1.8818888895161853E-4	0.0	0.0	9.064494214132608	0.0
24	1.8818888895161853E-4	0.0	0.0	9.21335162529334	0.0
25	1.8818888895161853E-4	0.0	0.0	9.338497236446166	0.0
26	1.8818888895161853E-4	0.0	0.0	9.461760958709476	0.0
27	1.8818888895161853E-4	0.0	0.0	9.602714436534239	0.0
28	1.8818888895161853E-4	0.0	0.0	9.718638792128434	0.0
29	1.8818888895161853E-4	0.0	0.0	9.869378092178682	0.0
30	1.8818888895161853E-4	0.0	0.0	10.072245714468526	0.0
31	1.8818888895161853E-4	0.0	0.0	10.211693681181675	0.0
32	1.8818888895161853E-4	0.0	0.0	10.352270781228535	0.0
33	1.8818888895161853E-4	0.0	0.0	10.490965992385878	0.0
34	1.8818888895161853E-4	0.0	0.0	10.65055017021685	0.0
35	1.8818888895161853E-4	0.0	0.0	10.86094534806476	0.0
36	1.8818888895161853E-4	0.0	0.0	11.031256292565974	0.0
37	1.8818888895161853E-4	0.0	0.0	11.191969603730657	0.0
38	1.8818888895161853E-4	0.0	0.0	11.403117537134372	0.0
39	1.8818888895161853E-4	0.0	0.0	11.82955355949874	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAA	30	2.1632895E-6	45.000004	33
GTATCGG	20	7.029857E-4	45.000004	2
CGTTTTT	14775	0.0	44.36041	1
GATACCT	640	0.0	41.48438	5
TACGGCT	555	0.0	41.351353	7
AGGCACG	55	6.002665E-11	40.90909	10
TGATACC	645	0.0	40.813953	4
CCGATGA	105	0.0	40.714287	18
GAATGAT	2200	0.0	40.397728	1
ACGGCTG	570	0.0	40.263157	8
GTTTTTT	16590	0.0	39.995476	2
CGACCAC	570	0.0	39.86842	12
AATGATA	2095	0.0	39.63007	2
TTACACG	290	0.0	39.56897	34
ATGATAC	2115	0.0	39.468086	3
TGATACG	1460	0.0	39.452057	4
CATACGA	80	0.0	39.375004	18
ACATACG	80	0.0	39.375004	17
CACCCGT	40	3.4535333E-7	39.375004	16
ATACCTG	675	0.0	39.333332	6
>>END_MODULE