##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933348.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544299
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.422159511591975	31.0	28.0	33.0	25.0	34.0
2	28.60517656655625	31.0	27.0	33.0	16.0	34.0
3	28.680390741118394	31.0	27.0	34.0	16.0	34.0
4	33.0617454744543	35.0	32.0	37.0	28.0	37.0
5	34.20994526905249	35.0	33.0	37.0	30.0	37.0
6	33.55830710693938	35.0	33.0	37.0	28.0	37.0
7	33.451117859852765	35.0	32.0	37.0	28.0	37.0
8	33.41102776231446	35.0	33.0	37.0	28.0	37.0
9	34.59143963152606	37.0	34.0	39.0	28.0	39.0
10	34.25283897269699	37.0	32.0	39.0	27.0	39.0
11	34.52393078069223	37.0	33.0	39.0	27.0	39.0
12	34.56593894164788	37.0	33.0	39.0	27.0	39.0
13	34.678195256651215	37.0	33.0	39.0	27.0	39.0
14	35.43344375058562	38.0	34.0	40.0	27.0	41.0
15	35.78054708900807	38.0	34.0	40.0	27.0	41.0
16	35.751930464689444	38.0	34.0	40.0	27.0	41.0
17	35.10394654408698	37.0	33.0	40.0	27.0	41.0
18	34.958414400908325	37.0	33.0	39.0	27.0	40.0
19	34.39397463526481	37.0	32.0	38.0	27.0	40.0
20	33.81760943892971	35.0	32.0	38.0	26.0	40.0
21	34.2376983973882	35.0	33.0	38.0	27.0	40.0
22	34.895309379587324	35.0	33.0	39.0	29.0	40.0
23	35.27808245100579	36.0	34.0	39.0	30.0	40.0
24	34.81778397535178	35.0	33.0	39.0	29.0	40.0
25	33.752725983329015	35.0	33.0	39.0	24.0	40.0
26	33.906090218795185	35.0	33.0	38.0	25.0	40.0
27	34.56464186044803	35.0	33.0	39.0	27.0	40.0
28	34.36314966590054	35.0	33.0	39.0	25.0	40.0
29	34.5189941557857	35.0	33.0	39.0	25.0	40.0
30	33.756773391095706	35.0	33.0	39.0	23.0	40.0
31	33.668320169612656	35.0	33.0	39.0	23.0	40.0
32	33.25858581404706	35.0	33.0	39.0	21.0	40.0
33	32.96335102581486	36.0	32.0	39.0	16.0	40.0
34	32.52281191036544	36.0	32.0	40.0	14.0	41.0
35	32.231075199476756	36.0	31.0	40.0	10.0	41.0
36	31.356162697340984	35.0	29.0	40.0	9.0	41.0
37	31.51827212616595	35.0	30.0	40.0	8.0	41.0
38	31.252416410833018	35.0	29.0	39.0	8.0	40.0
39	30.933756997532605	35.0	28.0	39.0	8.0	40.0
40	30.845024517774238	35.0	27.0	39.0	8.0	40.0
41	30.063579025498854	35.0	24.0	39.0	8.0	40.0
42	30.529813576728966	35.0	26.0	39.0	7.0	40.0
43	30.25799973911398	35.0	24.0	39.0	7.0	40.0
44	30.556293507796266	35.0	26.0	39.0	7.0	40.0
45	30.704225067472105	35.0	27.0	39.0	7.0	40.0
46	30.57151492102686	35.0	26.0	39.0	7.0	40.0
47	30.48732589991898	35.0	26.0	39.0	7.0	40.0
48	30.366934350421367	35.0	26.0	39.0	7.0	40.0
49	30.592543804048876	35.0	27.0	39.0	7.0	40.0
50	30.377408372971473	35.0	26.0	39.0	7.0	40.0
51	29.106889779330846	34.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	25.0
9	28.0
10	34.0
11	44.0
12	40.0
13	60.0
14	93.0
15	150.0
16	259.0
17	517.0
18	975.0
19	1860.0
20	2777.0
21	4234.0
22	6043.0
23	8792.0
24	12697.0
25	17839.0
26	22562.0
27	24184.0
28	23321.0
29	22513.0
30	24560.0
31	28424.0
32	33932.0
33	40469.0
34	45312.0
35	51774.0
36	57416.0
37	59922.0
38	46589.0
39	6852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.655940944223673	2.72846358343484	34.49684823966239	34.1187472326791
2	33.195541421167405	16.993968388698125	35.558948298637326	14.251541891497135
3	13.313638276021084	17.020240713284426	54.671788851348246	14.994332159346241
4	12.48192629418757	2.9924728871447495	67.4967251455542	17.028875673113493
5	22.75991688391858	3.5226961651592235	55.29460829433822	18.422778656583976
6	14.998190332886887	15.493138881386884	58.12485417022629	11.383816615499937
7	56.68777638761049	1.3836145206954265	38.83729347288899	3.091315618805105
8	56.23637008335493	11.260906229847933	29.093568057262644	3.409155629534502
9	52.976029718959616	2.8080154473919667	28.52457932129216	15.69137551235626
10	34.335723563703034	16.05882061146539	35.784927034589444	13.820528790242129
11	27.44631167795642	16.42020286643922	41.71953283030099	14.413952625303372
12	21.413046873134068	14.969897060255485	44.607100141650086	19.009955924960362
13	20.61220027962572	15.844416396135214	50.85146215591063	12.691921168328438
14	17.002052180878525	21.29601560906781	44.60011868476702	17.10181352528665
15	13.311249882876874	16.50728735492808	54.20550102057876	15.975961741616281
16	16.796834093026074	17.63424147389578	43.16726652079097	22.40165791228718
17	17.71893756924044	18.15142045089188	47.07853587825809	17.05110610160959
18	17.347083128941996	17.12955563026939	46.35889465165286	19.16446658913575
19	17.715263118249343	19.63369398069811	42.679666874273146	19.9713760267794
20	18.098508356620165	20.38126103483563	48.02084883492345	13.49938177362075
21	18.116145721377404	23.427380906450317	44.4869455942414	13.969527777930878
22	16.209473102100134	15.671166031905258	47.86321488740563	20.256145978588975
23	16.63074890822875	22.182476910668587	46.19703508549529	14.989739095607376
24	18.170343873496	18.87308262554221	42.475918566817136	20.480654934144653
25	16.302252989625188	25.90745160288738	40.570532005386745	17.219763402100682
26	15.554685935487663	17.031447788807256	45.80019437845743	21.613671897247652
27	18.387503927069496	19.155464184207577	42.93779705639731	19.519234832325616
28	13.566073059109057	19.931140788426948	47.27897717982212	19.223808972641876
29	19.044863209375727	18.371336342708695	44.31222544961501	18.271574998300565
30	19.967885298337862	21.66511421112293	43.52607666007103	14.840923830468181
31	19.722064527033854	17.832845549964265	43.459201652033165	18.985888270968715
32	23.343970868952542	23.929494634382937	38.30927486546916	14.417259631195353
33	22.953560451149094	18.36306882797874	37.53984482793465	21.14352589293752
34	22.826791891956443	20.07444437707951	41.11269724912226	15.986066481841782
35	23.682938972880716	22.71637463967415	35.40682602760615	18.193860359838983
36	20.79077859779276	21.26110832465244	41.27033119664008	16.67778188091472
37	21.92747001188685	25.812467044767672	36.0689620962008	16.191100847144675
38	24.01639540032225	22.01326844252883	35.29695994297252	18.6733762141764
39	23.4407926525678	24.370796198413007	34.25212980365571	17.936281345363486
40	26.377965052296627	20.82991150084788	35.03442041965904	17.75770302719645
41	21.90505586084119	22.836161741983727	36.00998715779379	19.248795239381298
42	21.27066189722928	21.749442861368475	38.70409462446192	18.275800616940323
43	21.12699086347761	20.76542488595423	36.99271907536115	21.114865175207008
44	23.67026211696145	21.264782775643535	34.19591070349202	20.869044403903004
45	18.847361468604575	21.486719615505447	34.93484279780048	24.731076118089508
46	22.911304264751543	24.60283777850042	32.83728244953601	19.64857550721203
47	17.67208831910402	21.601178763877943	41.28355922020801	19.443173696810025
48	20.69101725338463	24.97101776780777	32.5069493054369	21.8310156733707
49	20.134705373333407	21.49443596258674	37.879547822061035	20.491310842018816
50	20.203968774515477	20.851223316596208	36.33517607050536	22.60963183838295
51	20.118537788972603	21.84424369693863	33.508972090707495	24.528246423381265
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	104.0
1	767.5
2	1431.0
3	15331.0
4	29231.0
5	21340.0
6	13449.0
7	12823.5
8	12198.0
9	11917.5
10	11637.0
11	11152.0
12	10667.0
13	9918.5
14	9170.0
15	8599.5
16	8029.0
17	7413.0
18	6797.0
19	6349.0
20	5901.0
21	5667.5
22	5434.0
23	5441.5
24	5449.0
25	5421.5
26	5847.0
27	6300.0
28	6818.5
29	7337.0
30	7823.5
31	8310.0
32	9223.0
33	10136.0
34	11180.0
35	12224.0
36	12586.5
37	12949.0
38	13987.5
39	15026.0
40	15918.0
41	16810.0
42	17972.0
43	19134.0
44	21392.0
45	23650.0
46	52783.5
47	81917.0
48	58693.0
49	35469.0
50	32633.5
51	29798.0
52	26840.0
53	23882.0
54	23811.5
55	23741.0
56	23341.0
57	22941.0
58	21301.0
59	19661.0
60	17173.0
61	14685.0
62	12713.5
63	10742.0
64	9418.0
65	8094.0
66	6830.5
67	5567.0
68	4676.0
69	3785.0
70	3267.5
71	2750.0
72	2267.5
73	1785.0
74	1504.0
75	962.5
76	702.0
77	529.0
78	356.0
79	303.5
80	251.0
81	176.0
82	101.0
83	66.5
84	32.0
85	26.5
86	21.0
87	16.5
88	12.0
89	9.0
90	6.0
91	6.0
92	6.0
93	4.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	544299.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.725318070242544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.58340750837654	35.284023531198436
2	5.4797570031437575	4.682487218139122
3	2.3260072265483043	2.98138195763877
4	1.53860221228645	2.6294907561406964
5	1.1865062267218656	2.5346927964507504
6	0.9932891167191562	2.546315606852173
7	0.8513808943219168	2.546286365618201
8	0.7417914213973382	2.535461953678287
9	0.6519621735637509	2.5069762111750133
>10	3.551253939089486	23.41126350443195
>50	0.059102940204355844	1.7240005124199467
>100	0.03042063098698436	2.4577915759377804
>500	0.0017383217706848208	0.5215635368141742
>1k	0.0030420630986984364	3.2755081356136877
>5k	0.0	0.0
>10k+	0.0017383217706848208	10.362756337891023
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15289	2.8089340601397392	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG	15039	2.7630034227510984	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC	13213	2.4275260472644633	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC	12270	2.2542756830345083	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT	3215	0.5906679968179255	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTAGGGTT	3132	0.5754190252048966	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTC	2936	0.5394094054922018	No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	2557	0.4697785592110219	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTA	2421	0.4447922924716011	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCT	2370	0.4354224424443183	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGT	1010	0.18555977505011031	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTT	986	0.18115043386080076	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	634	0.11648009641759402	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTAT	608	0.11170331012917534	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATG	581	0.10674280129120209	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07771463846158086	0.0
2	0.0	0.0	0.0	0.6518476058195954	0.0
3	0.0	0.0	0.0	0.7547322335701517	0.0
4	0.0	0.0	0.0	1.4819060847071186	0.0
5	0.0	0.0	0.0	4.467030069869685	0.0
6	0.0	0.0	0.0	4.765211767796744	0.0
7	0.0	0.0	0.0	5.352205313623578	0.0
8	0.0	0.0	0.0	6.070927927481035	0.0
9	0.0	0.0	0.0	6.211843122989387	0.0
10	0.0	0.0	0.0	9.614752185839034	0.0
11	0.0	0.0	0.0	9.84477281788135	0.0
12	0.0	0.0	0.0	12.69283978107621	0.0
13	0.0	0.0	0.0	12.93314887589358	0.0
14	0.0	0.0	0.0	13.11264580680839	0.0
15	0.0	0.0	0.0	13.71396971150048	0.0
16	0.0	0.0	0.0	14.02225614965304	0.0
17	0.0	0.0	0.0	14.172173750089565	0.0
18	0.0	0.0	0.0	14.311986610300588	0.0
19	0.0	0.0	0.0	15.154170777458713	0.0
20	0.0	0.0	0.0	15.345058506445906	0.0
21	0.0	0.0	0.0	15.49093421079223	0.0
22	0.0	0.0	0.0	15.784155399881316	0.0
23	0.0	0.0	0.0	15.932786942470958	0.0
24	0.0	0.0	0.0	16.067455571294452	0.0
25	0.0	0.0	0.0	16.173279759837882	0.0
26	0.0	0.0	0.0	16.319155464184206	0.0
27	0.0	0.0	0.0	16.50471523923432	0.0
28	0.0	0.0	0.0	16.620827890552803	0.0
29	0.0	0.0	0.0	16.756415132124072	0.0
30	0.0	0.0	0.0	16.92470498751605	0.0
31	0.0	0.0	0.0	17.06029222908732	0.0
32	0.0	0.0	0.0	17.20469815303721	0.0
33	0.0	0.0	0.0	17.34726685149155	0.0
34	0.0	0.0	0.0	17.567182743308365	0.0
35	0.0	0.0	0.0	17.782873016485425	0.0
36	0.0	0.0	0.0	17.95171403952607	0.0
37	0.0	0.0	0.0	18.137641259675288	0.0
38	0.0	0.0	0.0	18.374643348600678	0.0
39	0.0	0.0	0.0	18.828989213649116	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTTA	20	7.0300105E-4	45.0	32
TAACACG	25	3.88809E-5	45.0	33
AACACGC	20	7.0300105E-4	45.0	34
ATGCGTT	30	2.1633878E-6	44.999996	12
CGTTTTT	11145	0.0	44.131897	1
TGATACC	1955	0.0	43.15857	4
GATACCT	1955	0.0	43.04348	5
CCGATGA	450	0.0	43.0	18
CGTTCAT	75	0.0	42.0	17
TACGGCT	1395	0.0	41.774197	7
ATACCTG	2045	0.0	41.149143	6
ACGGCTG	1415	0.0	41.024734	8
CACCGGT	110	0.0	40.909092	16
TACCTGT	2090	0.0	40.47847	7
GAATGAT	6100	0.0	40.09426	1
AATGATA	5750	0.0	39.95217	2
ATGATAC	5845	0.0	39.91873	3
CGGCTGT	1485	0.0	39.696968	9
TCGTTCA	80	0.0	39.375	16
GAATCTG	1635	0.0	38.944954	1
>>END_MODULE