##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933347.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	403469
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.559215206124883	31.0	28.0	31.0	16.0	33.0
2	28.914104925037613	31.0	30.0	33.0	16.0	34.0
3	29.139418393978225	31.0	30.0	33.0	16.0	34.0
4	31.99006862980799	35.0	30.0	37.0	19.0	37.0
5	33.58648371993883	35.0	33.0	37.0	28.0	37.0
6	32.68030257591042	35.0	33.0	37.0	17.0	37.0
7	31.83932594573561	35.0	30.0	35.0	25.0	37.0
8	32.847475270714725	35.0	32.0	35.0	27.0	37.0
9	32.72996438388079	35.0	31.0	37.0	25.0	39.0
10	30.797379724340654	35.0	27.0	38.0	15.0	39.0
11	31.634831920172306	35.0	27.0	39.0	15.0	39.0
12	32.58650106947498	35.0	30.0	39.0	21.0	39.0
13	32.623428317912904	35.0	30.0	39.0	21.0	39.0
14	32.907799607900486	36.0	31.0	39.0	17.0	41.0
15	33.58921007561919	36.0	31.0	39.0	22.0	41.0
16	33.85512889466105	36.0	32.0	40.0	23.0	41.0
17	31.88666539436735	34.0	27.0	39.0	18.0	41.0
18	32.70711752327936	35.0	30.0	39.0	21.0	39.0
19	32.56252153201361	35.0	30.0	37.0	19.0	39.0
20	32.289586064852564	34.0	30.0	37.0	23.0	39.0
21	32.62882154514969	35.0	31.0	37.0	23.0	39.0
22	33.01700502392997	35.0	31.0	38.0	25.0	39.0
23	34.10911866834874	35.0	33.0	38.0	27.0	40.0
24	32.35074317977342	35.0	31.0	37.0	22.0	39.0
25	31.51027464315722	34.0	30.0	37.0	18.0	39.0
26	30.974501138873123	33.0	27.0	37.0	20.0	39.0
27	32.554154098580064	35.0	30.0	38.0	22.0	39.0
28	32.54625758112767	35.0	31.0	38.0	22.0	39.0
29	32.94230535679321	35.0	31.0	38.0	22.0	40.0
30	31.794943353764477	35.0	30.0	38.0	18.0	40.0
31	32.08496315702074	35.0	30.0	38.0	21.0	40.0
32	31.930358961902897	35.0	30.0	38.0	19.0	40.0
33	32.1175753279682	35.0	31.0	38.0	18.0	40.0
34	31.41417308392962	35.0	30.0	38.0	15.0	40.0
35	30.948070359804596	35.0	29.0	38.0	10.0	40.0
36	30.21616282787525	35.0	27.0	38.0	9.0	40.0
37	30.4100959429349	35.0	27.0	38.0	9.0	40.0
38	30.35120913874424	35.0	26.0	38.0	10.0	40.0
39	29.930646468501916	34.0	25.0	38.0	8.0	40.0
40	29.85471002728834	35.0	25.0	38.0	8.0	40.0
41	29.112749678413955	34.0	23.0	38.0	8.0	40.0
42	29.73845574257254	34.0	24.0	38.0	8.0	40.0
43	29.56466543898044	34.0	24.0	38.0	8.0	40.0
44	29.72544854747205	34.0	24.0	38.0	7.0	40.0
45	30.016266429391106	35.0	25.0	38.0	7.0	40.0
46	29.82118824494571	35.0	24.0	38.0	7.0	40.0
47	29.88202811120557	35.0	25.0	38.0	7.0	40.0
48	29.61933382738203	34.0	24.0	38.0	7.0	40.0
49	29.446983535290197	34.0	24.0	37.0	7.0	40.0
50	29.474494942610214	34.0	24.0	38.0	7.0	40.0
51	27.832036662048385	32.0	22.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	14.0
10	29.0
11	22.0
12	34.0
13	60.0
14	106.0
15	219.0
16	431.0
17	819.0
18	1350.0
19	2142.0
20	3103.0
21	4423.0
22	6153.0
23	8438.0
24	11469.0
25	15347.0
26	19459.0
27	21081.0
28	21403.0
29	22826.0
30	25926.0
31	29683.0
32	33453.0
33	37572.0
34	41646.0
35	44028.0
36	31997.0
37	16793.0
38	3385.0
39	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.99309240610803	2.7684902681494736	37.18352587187616	37.05489145386635
2	37.14188698512154	10.84940850474262	37.811083379392215	14.197621130743626
3	14.090549707660315	10.779762509635189	60.16050799441841	14.969179788286088
4	13.165571580468388	3.0155972329968352	66.50870327088326	17.310127915651513
5	18.970726375508402	3.508819760625971	59.86556587990651	17.654887983959114
6	15.307495743167381	10.448138518696604	61.56061556154252	12.683750176593492
7	56.604844486193485	1.421670562050616	38.6245287742057	3.348956177550196
8	58.27932257496858	7.312581635763839	30.716362347540947	3.691733441726626
9	54.17045671414657	2.698100721492853	31.248497406244347	11.882945158116238
10	32.05723364124629	16.287496684999343	37.92881237468058	13.726457299073783
11	24.966478217657368	16.81814464060436	44.35408916174477	13.861287979993506
12	20.855629552704173	15.425224738455743	46.91760705283429	16.80153865600579
13	19.34027149545566	16.269155746785998	51.49986739997373	12.890705357784613
14	16.469666814550806	17.979076459405803	48.10431532534098	17.44694140070241
15	13.203740559993458	16.64464927912677	54.32709824050919	15.824511920370584
16	16.449838773239083	15.928609137257139	46.67074793850333	20.950804151000447
17	17.558721983597252	16.56137150561753	48.780699384587166	17.099207126198046
18	16.779727810562896	15.639367584622363	49.08357271562375	18.497331889190992
19	17.62093246321279	18.224944171671183	45.513533877447834	18.640589487668198
20	17.492793746235773	19.245840448708577	49.29077574733127	13.970590057724387
21	18.09209629488263	20.472204803838707	46.48461220068952	14.951086700589142
22	16.328887721237567	16.07310598831633	48.946511380056464	18.651494910389648
23	16.300632762368362	20.50814312871621	47.3481233998151	15.843100709100327
24	16.705620506160322	17.82565698975634	45.68331148117947	19.785411022903865
25	15.992058869454654	21.305230389447516	44.07203527408549	18.63067546701234
26	15.331289392741448	17.47346140595684	47.47997987453807	19.715269326763643
27	16.448351670140703	18.30549558950006	46.30244207113805	18.94371066922118
28	13.835511526288267	18.667109492922627	49.25781162865053	18.23956735213858
29	17.837305964026974	17.80335044328065	46.47048472125492	17.888858871437456
30	18.446026832296905	19.768309337272505	46.14728764787381	15.638376182556776
31	18.86613345758906	18.04326974315251	45.55814697039921	17.532449828859217
32	21.39049096708793	21.622231199918705	41.97868981260022	15.008588020393141
33	20.670733067472348	18.369688873246766	41.66020189903066	19.29937616025023
34	20.366868334370174	19.68726221841083	43.46728001407791	16.47858943314108
35	20.88115815589302	22.158084016368047	38.86643087820873	18.0943269495302
36	20.373312447796486	20.436762179994002	42.39136092240049	16.79856444980903
37	20.51805714937207	24.435582411535954	39.029020816964874	16.017339622127103
38	22.083481010932687	21.97244397958703	38.6460917691322	17.29798324034808
39	21.729302623002013	22.99854511746875	37.86932825074541	17.40282400878382
40	24.383781653609073	20.451137509945003	37.859909931122345	17.305170905323582
41	20.80258954219531	21.204107378757726	39.32098872528001	18.672314353766957
42	20.1135651066129	20.91759218180331	40.68168806029707	18.287154651286716
43	19.8548091674949	20.299948694943108	39.88484865008216	19.96039348747983
44	22.002186041554616	20.82440038763821	37.52308107933943	19.650332491467744
45	18.440326270419785	21.27994963677507	37.916915549893545	22.3628085429116
46	20.691552510849657	23.428813613933166	36.569847993278294	19.309785881938886
47	17.81202521135453	21.175108868339326	41.879797456558	19.133068463748142
48	19.732866713427796	23.159895803642907	36.42857319893226	20.678664283997037
49	19.552431537491106	20.921805640582054	39.31627956546848	20.209483256458366
50	19.625547439828093	20.69973157789074	38.28621281932441	21.388508162956757
51	19.664707821418745	20.81349496491676	36.58546257581128	22.936334637853218
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	123.0
1	419.5
2	716.0
3	17573.0
4	34430.0
5	22680.0
6	10930.0
7	10390.0
8	9850.0
9	9545.0
10	9240.0
11	8765.5
12	8291.0
13	7672.5
14	7054.0
15	6446.5
16	5839.0
17	5257.5
18	4676.0
19	4286.5
20	3897.0
21	3811.5
22	3726.0
23	3693.5
24	3661.0
25	3563.5
26	3834.0
27	4202.0
28	4550.0
29	4898.0
30	5392.0
31	5886.0
32	6418.0
33	6950.0
34	7777.0
35	8604.0
36	9010.5
37	9417.0
38	10231.0
39	11045.0
40	12038.5
41	13032.0
42	13950.0
43	14868.0
44	16434.0
45	18000.0
46	29648.5
47	41297.0
48	35437.0
49	29577.0
50	27572.0
51	25567.0
52	22579.5
53	19592.0
54	18423.0
55	17254.0
56	16376.0
57	15498.0
58	14257.0
59	13016.0
60	11603.0
61	10190.0
62	9098.0
63	8006.0
64	7111.0
65	6216.0
66	5387.0
67	4558.0
68	3963.5
69	3369.0
70	2877.0
71	2385.0
72	2008.5
73	1632.0
74	1388.5
75	894.0
76	643.0
77	490.0
78	337.0
79	269.0
80	201.0
81	151.0
82	101.0
83	69.5
84	38.0
85	30.0
86	22.0
87	18.5
88	15.0
89	10.0
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	403469.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.82207329084876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.22717214560339	35.157210176821714
2	6.751543957711598	5.917333082824495
3	2.958723587844183	3.8897220574161238
4	1.8872390733703406	3.3081091596235424
5	1.4205706666848794	3.1126175935147335
6	1.1040430595704682	2.9028873523649974
7	0.929756961042414	2.852071438263424
8	0.7657862574816291	2.6846673200387783
9	0.6522193680458791	2.5723444453395814
>10	3.2106932583741	21.019544032149437
>50	0.056504144366067564	1.707770842797426
>100	0.02940521798642292	2.6024618554243952
>500	0.002306291606778268	0.7852865482193223
>1k	0.002306291606778268	2.0445748868052624
>5k	0.001153145803389134	3.0592823442212214
>10k+	5.76572901694567E-4	6.384116864175553
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25267	6.2624389977916515	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG	7098	1.7592429653827184	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC	5010	1.2417310871467202	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC	3916	0.9705826222088934	No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	1465	0.36310100652094707	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTAGGGTT	1429	0.35417838793067125	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT	1282	0.31774436202037826	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTC	971	0.24066285142105093	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTA	853	0.21141649048625794	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCT	657	0.16283778927253395	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTGTAGGGTTCGTATGCCG	627	0.15540227378063742	No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	496	0.12293385613268924	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14771890777234434	0.0
2	0.0	0.0	0.0	0.7745328637392216	0.0
3	0.0	0.0	0.0	0.9760353335696175	0.0
4	0.0	0.0	0.0	1.5797991915116154	0.0
5	0.0	0.0	0.0	2.9942820885867314	0.0
6	0.0	0.0	0.0	3.3474690744518165	0.0
7	0.0	0.0	0.0	3.7559267254733326	0.0
8	0.0	0.0	0.0	4.330692072996934	0.0
9	0.0	0.0	0.0	4.57532053268033	0.0
10	0.0	0.0	0.0	7.074892990539546	0.0
11	0.0	0.0	0.0	7.71558657542463	0.0
12	0.0	0.0	0.0	9.663195933268726	0.0
13	0.0	0.0	0.0	9.924678228067089	0.0
14	0.0	0.0	0.0	10.087268166823225	0.0
15	0.0	0.0	0.0	10.553474988165139	0.0
16	0.0	0.0	0.0	10.839246633570362	0.0
17	0.0	0.0	0.0	11.120061268647653	0.0
18	0.0	0.0	0.0	11.424669553299013	0.0
19	0.0	0.0	0.0	12.099814359963219	0.0
20	0.0	0.0	0.0	12.390543015696373	0.0
21	0.0	0.0	0.0	12.7120051354627	0.0
22	0.0	0.0	0.0	13.144999987607473	0.0
23	0.0	0.0	0.0	13.466709957890197	0.0
24	0.0	0.0	0.0	13.720756737196663	0.0
25	0.0	0.0	0.0	13.937625939043643	0.0
26	0.0	0.0	0.0	14.14259831610359	0.0
27	0.0	0.0	0.0	14.380782662360676	0.0
28	0.0	0.0	0.0	14.581045879609091	0.0
29	0.0	0.0	0.0	14.811051158825089	0.0
30	0.0	0.0	0.0	15.080712520664537	0.0
31	0.0	0.0	0.0	15.299316676126296	0.0
32	0.0	0.0	0.0	15.507015408866604	0.0
33	0.0	0.0	0.0	15.721158255033224	0.0
34	0.0	0.0	0.0	15.998255132364568	0.0
35	0.0	0.0	0.0	16.30435052011431	0.0
36	0.0	0.0	0.0	16.550466082896083	0.0
37	0.0	0.0	0.0	16.786915475538393	0.0
38	0.0	0.0	0.0	17.072934971460015	0.0
39	0.0	0.0	0.0	17.626880875606304	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACACT	20	7.027838E-4	45.0	18
TTGCACG	25	3.8862905E-5	45.0	1
TGCACGG	25	3.8862905E-5	45.0	2
CGTTTTT	9750	0.0	44.307693	1
ACCGGTG	100	0.0	42.75	17
ACGGCTG	760	0.0	42.33553	8
CCGATGA	145	0.0	41.896553	18
TACGGCT	770	0.0	41.785717	7
ATCCGGC	65	0.0	41.53846	6
CAAGACG	65	0.0	41.53846	38
GGCCGAT	125	0.0	41.399998	8
GATACCT	905	0.0	41.270714	5
TGATACC	915	0.0	40.81967	4
GCGACCA	635	0.0	40.748035	11
ATGATAC	2695	0.0	40.57514	3
TTTCGCG	100	0.0	40.5	1
GAATGAT	2900	0.0	40.422417	1
CGACCAC	630	0.0	40.357143	12
AATGATA	2710	0.0	40.1845	2
CGGCTGT	795	0.0	39.905663	9
>>END_MODULE