##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933345.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	216887
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.472241305380223	31.0	27.0	31.0	16.0	33.0
2	28.495460769894	31.0	28.0	33.0	16.0	34.0
3	28.61759810408185	31.0	28.0	33.0	16.0	34.0
4	32.097211912193906	35.0	30.0	37.0	19.0	37.0
5	33.792071447343545	35.0	33.0	37.0	30.0	37.0
6	32.514161752433296	35.0	33.0	37.0	17.0	37.0
7	32.63190048273986	35.0	32.0	36.0	27.0	37.0
8	32.86747015727084	35.0	32.0	37.0	26.0	37.0
9	33.38417240314081	35.0	32.0	38.0	26.0	39.0
10	32.008373023740475	35.0	28.0	39.0	15.0	39.0
11	32.74607975581755	37.0	30.0	39.0	17.0	39.0
12	33.389972658573356	37.0	31.0	39.0	23.0	39.0
13	33.49935680792302	37.0	32.0	39.0	23.0	39.0
14	33.6149192897684	37.0	32.0	40.0	18.0	41.0
15	34.23831303858692	37.0	32.0	40.0	23.0	41.0
16	34.50490347508149	37.0	32.0	40.0	25.0	41.0
17	32.52089797913199	36.0	27.0	40.0	18.0	41.0
18	33.18188273156068	36.0	30.0	39.0	23.0	39.0
19	32.94440883962616	36.0	31.0	37.0	23.0	39.0
20	32.26985480918635	34.0	30.0	35.0	24.0	39.0
21	32.63914388598671	35.0	31.0	36.0	24.0	39.0
22	32.73541982691447	34.0	30.0	37.0	25.0	39.0
23	33.769797175487696	35.0	32.0	38.0	27.0	39.0
24	32.14102274456284	34.0	31.0	36.0	21.0	39.0
25	31.304292096806172	34.0	29.0	36.0	18.0	39.0
26	30.30943763342201	33.0	25.0	36.0	19.0	39.0
27	32.260651860185256	34.0	30.0	37.0	22.0	39.0
28	32.22516333390199	35.0	31.0	37.0	21.0	39.0
29	32.33439994098309	35.0	31.0	37.0	22.0	39.0
30	31.408779687118177	34.0	30.0	37.0	18.0	39.0
31	31.67078709189578	34.0	30.0	36.0	21.0	39.0
32	31.64171204359874	35.0	30.0	37.0	18.0	39.0
33	31.41028738467497	35.0	30.0	37.0	16.0	39.0
34	30.28268637585471	35.0	27.0	37.0	12.0	39.0
35	29.621489531414976	34.0	24.0	37.0	9.0	39.0
36	28.530534333546964	34.0	21.0	37.0	7.0	39.0
37	28.563814336497806	34.0	21.0	37.0	7.0	40.0
38	28.331066407852937	34.0	20.0	37.0	7.0	40.0
39	27.90250683535666	33.0	18.0	37.0	7.0	39.0
40	27.6823783813691	33.0	18.0	37.0	7.0	39.0
41	27.14379838349002	33.0	18.0	36.0	7.0	39.0
42	27.755278094122747	33.0	18.0	37.0	7.0	39.0
43	27.522013767537935	33.0	18.0	37.0	7.0	39.0
44	27.333113556829133	33.0	15.0	37.0	7.0	40.0
45	27.47212142728702	34.0	15.0	37.0	7.0	40.0
46	27.10750298542559	33.0	15.0	37.0	7.0	40.0
47	27.143434138514525	33.0	15.0	37.0	7.0	40.0
48	26.97956539580519	33.0	15.0	37.0	7.0	40.0
49	26.94930539866382	33.0	13.0	37.0	7.0	39.0
50	27.025879836043654	33.0	12.0	37.0	7.0	40.0
51	25.570647387810244	31.0	10.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	9.0
11	13.0
12	19.0
13	26.0
14	43.0
15	85.0
16	209.0
17	393.0
18	748.0
19	1243.0
20	1942.0
21	2790.0
22	3942.0
23	5623.0
24	7497.0
25	10475.0
26	13846.0
27	14949.0
28	14078.0
29	13414.0
30	14114.0
31	15233.0
32	16978.0
33	17930.0
34	19390.0
35	20533.0
36	13509.0
37	6536.0
38	1290.0
39	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.959882335040827	2.257396708885272	30.78146684679119	44.00125410928271
2	44.821035838939174	13.961187161978359	29.88653077408973	11.33124622499274
3	10.84712315629798	13.791052483551342	63.92360999045585	11.438214369694819
4	9.612839865920964	2.2034515669449988	74.42032025893668	13.763388308197355
5	19.1417650666015	2.907504829703947	64.02642850885483	13.924301594839708
6	11.24825369893078	12.540631757551168	65.67936298625551	10.531751557262538
7	44.66381110901068	0.8598947839197371	51.507467021997634	2.9688270850719496
8	45.21294498978731	9.83830289505595	41.1513829782329	3.797369136923836
9	42.08643210519764	2.082651334565926	41.324745143784554	14.506171416451885
10	26.884045609003763	13.472914466980502	46.71741505945492	12.925624864560808
11	21.67027069395584	13.562823036880955	53.3065605591852	11.460345709978007
12	18.14401047550107	12.37003600953492	54.1715271085865	15.314426406377516
13	14.76390931683319	15.452286213558212	60.01604522170532	9.767759247903287
14	12.453950674775344	18.591247977057176	52.720541111269924	16.234260236897555
15	8.844236860669382	13.96764213622762	63.71290118817633	13.475219814926668
16	10.419250577489661	13.670713320761502	54.120348384181625	21.78968771756721
17	10.469507162716068	14.71042524448215	58.37279320567853	16.447274387123247
18	11.2067574358998	13.85145259974088	57.86146703121903	17.080322933140298
19	12.421214733939793	17.31039665816762	53.88658610244044	16.381802505452146
20	13.359030278439926	16.239793071968354	59.288016340306235	11.11316030928548
21	13.454471683411176	20.846339337996284	52.86439482311065	12.834794155481887
22	11.020024252260393	14.18157842563178	56.081738416779245	18.716658905328583
23	11.53227256589837	20.897518062401158	52.85240701379059	14.717802357909878
24	12.712610714335115	15.02395256516066	53.48407235103995	18.779364369464282
25	10.164740164232988	21.859309225541413	52.191694292419555	15.784256317806047
26	10.742921429131298	17.372179983124852	55.426097460889764	16.458801126854077
27	12.731053497904437	18.36117425202986	52.17694006556409	16.730832184501608
28	8.965498162637688	16.809675084260466	59.47889915024874	14.745927602853099
29	14.36416198296809	15.59014602073891	53.40430731210262	16.641384684190385
30	12.918247751133077	19.56502694951749	55.24628032108886	12.270444978260569
31	14.626971648830958	15.035479304891489	51.63887185492907	18.69867719134849
32	16.25270302046688	19.18741095593558	48.92086662639992	15.639019397197618
33	12.868913305085137	14.99398304186051	50.60976453176078	21.527339121293576
34	14.155758528634726	20.791933126466777	46.51592764896006	18.536380695938437
35	15.02072507803603	18.431717899182523	45.46100042879472	21.08655659398673
36	15.006892990359036	19.638798083794786	48.83787410033796	16.516434825508213
37	15.63302549253759	18.983618197494547	45.619608367490905	19.763747942476957
38	13.910469507162714	19.131621535638374	49.409600390987016	17.548308566211897
39	14.1414653713685	19.571942993355986	43.92010586157768	22.366485773697825
40	14.47113012767017	18.114040952200916	46.17335294415986	21.241475975969053
41	13.427268577646423	19.40134724533974	45.60070451433235	21.57067966268149
42	13.520865704260743	18.50133940715672	46.5624956774726	21.415299211109932
43	15.31212105843135	18.96609755310369	45.06863020835735	20.653151180107614
44	18.40174837588237	19.949097917348666	41.716193225043455	19.932960481725505
45	13.9395168912844	21.43143664673309	41.288320646235135	23.34072581574737
46	17.986324675983344	23.375306034939854	38.08803662736817	20.55033266170863
47	13.042275470636783	19.72870665369524	46.57678883473883	20.65222904092915
48	16.839183537971387	21.268218012144573	37.804017760400576	24.088580689483464
49	14.223535758251993	17.800974701111638	43.51528676223103	24.460202778405346
50	16.043838496544282	17.760861646848358	41.52484934551172	24.67045051109564
51	16.196913600169673	18.21732054018913	39.01155901460208	26.57420684503912
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15.0
1	122.0
2	229.0
3	11321.0
4	22413.0
5	15648.5
6	8884.0
7	9169.5
8	9455.0
9	9303.5
10	9152.0
11	8291.0
12	7430.0
13	6466.5
14	5503.0
15	4827.0
16	4151.0
17	3733.0
18	3315.0
19	3026.0
20	2737.0
21	2556.5
22	2376.0
23	2245.0
24	2114.0
25	2028.0
26	1920.5
27	1899.0
28	2054.0
29	2209.0
30	2276.5
31	2344.0
32	2725.0
33	3106.0
34	3053.0
35	3000.0
36	3349.0
37	3698.0
38	3962.0
39	4226.0
40	5218.5
41	6211.0
42	6866.0
43	7521.0
44	8370.5
45	9220.0
46	18078.0
47	26936.0
48	22505.5
49	18075.0
50	16257.5
51	14440.0
52	11933.5
53	9427.0
54	7929.5
55	6432.0
56	5849.0
57	5266.0
58	4576.5
59	3887.0
60	3427.5
61	2968.0
62	2537.0
63	2106.0
64	1724.5
65	1343.0
66	1142.5
67	942.0
68	793.0
69	644.0
70	584.5
71	525.0
72	424.0
73	323.0
74	258.5
75	149.5
76	105.0
77	97.5
78	90.0
79	56.5
80	23.0
81	13.0
82	3.0
83	3.5
84	4.0
85	3.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	216887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.052031703144955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.49230391354183	37.614979228815
2	6.361552317013263	5.732017133345936
3	2.473595873587686	3.34321559152923
4	1.4716718519731455	2.652072277268808
5	1.0868675290650074	2.4482795188277766
6	0.8125921074177175	2.1965355231065025
7	0.6918290486327165	2.1817812962510432
8	0.5495742590469952	1.9807549553454102
9	0.48919272965449484	1.9835213728808088
>10	2.425495333224169	17.51418941660865
>50	0.09108400196495824	2.803764172126499
>100	0.04503029310627149	4.000700825775634
>500	0.0030702472572457836	0.9839225034234418
>1k	0.0040936630096610445	4.377394680179079
>5k	0.0010234157524152611	2.5243560010512387
>10k+	0.0010234157524152611	7.662515503464938
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16619	7.662515503464938	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCG	5475	2.5243560010512387	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGC	4429	2.042077210713413	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTC	2653	1.2232176202354221	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCTCCTT	1240	0.5717262906490477	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	1172	0.5403735585811966	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT	811	0.37392743686804647	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTA	712	0.3282815475339693	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTC	611	0.2817135190214259	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCT	478	0.2203912636534232	No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT	432	0.19918206254870047	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGTCTCCTTCGTATGCCG	416	0.19180494912097085	No Hit
TCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	365	0.16829040007008259	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	338	0.1558415211607888	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	304	0.1401651551268633	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGT	290	0.13371018087759984	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT	282	0.13002162416373503	No Hit
GAATCTTTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTC	279	0.12863841539603574	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	256	0.11803381484367435	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	245	0.11296204936211021	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCC	236	0.1088124230590123	No Hit
GAATGACTCTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCT	217	0.10005210086358335	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCAGTCTCCTTCGTATGC	217	0.10005210086358335	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6106958923310294E-4	0.0	0.0	0.25404934366743975	0.0
2	4.6106958923310294E-4	0.0	0.0	1.2826955972464924	0.0
3	4.6106958923310294E-4	0.0	0.0	1.5708640905171818	0.0
4	4.6106958923310294E-4	0.0	0.0	2.3809633587997436	0.0
5	4.6106958923310294E-4	0.0	0.0	4.25106161272921	0.0
6	4.6106958923310294E-4	0.0	0.0	4.600552361367901	0.0
7	4.6106958923310294E-4	0.0	0.0	5.115567092541277	0.0
8	4.6106958923310294E-4	0.0	0.0	5.765214143770719	0.0
9	4.6106958923310294E-4	0.0	0.0	6.002203912636534	0.0
10	4.6106958923310294E-4	0.0	0.0	10.207619636031666	0.0
11	4.6106958923310294E-4	0.0	0.0	11.272690387160134	0.0
12	4.6106958923310294E-4	0.0	0.0	14.336958877203337	0.0
13	4.6106958923310294E-4	0.0	0.0	14.699359574340555	0.0
14	4.6106958923310294E-4	0.0	0.0	14.908224098263151	0.0
15	4.6106958923310294E-4	0.0	0.0	15.549571896886397	0.0
16	4.6106958923310294E-4	0.0	0.0	15.956235274589995	0.0
17	4.6106958923310294E-4	0.0	0.0	16.391946036415277	0.0
18	4.6106958923310294E-4	0.0	0.0	16.814285780152797	0.0
19	4.6106958923310294E-4	0.0	0.0	17.85123128633805	0.0
20	4.6106958923310294E-4	0.0	0.0	18.261122151166276	0.0
21	4.6106958923310294E-4	0.0	0.0	18.697293982580792	0.0
22	4.6106958923310294E-4	0.0	0.0	19.36584488696879	0.0
23	4.6106958923310294E-4	0.0	0.0	19.822764849898796	0.0
24	4.6106958923310294E-4	0.0	0.0	20.163034206752826	0.0
25	4.6106958923310294E-4	0.0	0.0	20.43414312522189	0.0
26	4.6106958923310294E-4	0.0	0.0	20.68680926012163	0.0
27	4.6106958923310294E-4	0.0	0.0	20.956534969822997	0.0
28	4.6106958923310294E-4	0.0	0.0	21.186147625261082	0.0
29	4.6106958923310294E-4	0.0	0.0	21.463250448390177	0.0
30	4.6106958923310294E-4	0.0	0.0	21.765251029337858	0.0
31	4.6106958923310294E-4	0.0	0.0	22.04373706123465	0.0
32	4.6106958923310294E-4	0.0	0.0	22.30378030956212	0.0
33	4.6106958923310294E-4	0.0	0.0	22.5513746789803	0.0
34	4.6106958923310294E-4	0.0	0.0	22.809573648950835	0.0
35	4.6106958923310294E-4	0.0	0.0	23.07607187152757	0.0
36	4.6106958923310294E-4	0.0	0.0	23.320438753821115	0.0
37	4.6106958923310294E-4	0.0	0.0	23.564344566525424	0.0
38	4.6106958923310294E-4	0.0	0.0	23.821621397317497	0.0
39	4.6106958923310294E-4	0.0	0.0	24.142525831423736	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	20	7.020617E-4	45.000004	21
ACACGAC	20	7.020617E-4	45.000004	26
GCTGGTA	20	7.020617E-4	45.000004	34
TTAGCGG	20	7.020617E-4	45.000004	2
GACACGA	20	7.020617E-4	45.000004	25
GGGTATG	20	7.020617E-4	45.000004	7
GGACTGC	20	7.020617E-4	45.000004	8
CTAAGTC	20	7.020617E-4	45.000004	45
AAGCGGC	20	7.020617E-4	45.000004	22
TACGGGC	20	7.020617E-4	45.000004	4
AGCGGCT	20	7.020617E-4	45.000004	23
CGGGCGA	80	0.0	45.000004	6
TTGAAGG	30	2.1573323E-6	45.000004	34
CAGTGTT	20	7.020617E-4	45.000004	31
CGGGATT	40	6.7702786E-9	45.000004	6
TAGGGTC	20	7.020617E-4	45.000004	5
AAACACG	20	7.020617E-4	45.000004	40
CTATTAC	30	2.1573323E-6	45.000004	37
CCCGGGT	20	7.020617E-4	45.000004	4
TCAAGCG	20	7.020617E-4	45.000004	17
>>END_MODULE