##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933344.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	477910
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.51322215479902	31.0	28.0	33.0	25.0	34.0
2	28.855114979807915	31.0	28.0	33.0	16.0	34.0
3	28.887215166035446	31.0	28.0	34.0	16.0	34.0
4	33.150752233684166	35.0	32.0	37.0	28.0	37.0
5	34.192822916448705	35.0	33.0	37.0	30.0	37.0
6	33.73801971082421	35.0	33.0	37.0	28.0	37.0
7	33.40314494360863	35.0	32.0	37.0	28.0	37.0
8	33.544470716243644	35.0	33.0	37.0	28.0	37.0
9	34.58770270553033	37.0	34.0	39.0	28.0	39.0
10	34.22814755916386	37.0	32.0	39.0	27.0	39.0
11	34.371582515536396	37.0	32.0	39.0	27.0	39.0
12	34.53289949990584	37.0	33.0	39.0	27.0	39.0
13	34.69505136950472	37.0	33.0	39.0	27.0	39.0
14	35.54754033186165	38.0	34.0	40.0	27.0	41.0
15	35.88229164487037	38.0	34.0	40.0	27.0	41.0
16	35.75963675168965	38.0	34.0	40.0	27.0	41.0
17	35.10608482768722	37.0	33.0	40.0	27.0	41.0
18	34.894787721537526	37.0	33.0	39.0	27.0	40.0
19	34.28124542277835	37.0	32.0	38.0	27.0	40.0
20	33.93666380699295	35.0	32.0	38.0	27.0	40.0
21	34.23522211295014	35.0	33.0	38.0	27.0	40.0
22	34.8400682136804	35.0	33.0	39.0	29.0	40.0
23	35.26641836329016	35.0	34.0	39.0	30.0	40.0
24	34.759680693017515	35.0	33.0	39.0	28.0	40.0
25	33.71260069887636	35.0	33.0	38.0	24.0	40.0
26	33.80224100772112	35.0	32.0	38.0	25.0	40.0
27	34.4765416082526	35.0	33.0	39.0	27.0	40.0
28	34.333776234018956	35.0	33.0	39.0	25.0	40.0
29	34.37685128999184	35.0	33.0	39.0	25.0	40.0
30	33.646747295515894	35.0	33.0	39.0	23.0	40.0
31	33.510619154234064	35.0	33.0	39.0	23.0	40.0
32	33.10594463392689	35.0	32.0	39.0	20.0	40.0
33	32.78324998430667	35.0	32.0	39.0	16.0	40.0
34	32.361015672406936	36.0	31.0	39.0	12.0	41.0
35	32.094781444204976	36.0	31.0	40.0	10.0	41.0
36	31.514301855997992	35.0	30.0	40.0	8.0	41.0
37	31.514159569793474	36.0	30.0	40.0	8.0	41.0
38	31.21636709840765	35.0	29.0	39.0	8.0	40.0
39	30.894078382959133	35.0	28.0	39.0	8.0	40.0
40	30.71282877529242	35.0	27.0	39.0	8.0	40.0
41	30.19549496767174	35.0	24.0	39.0	8.0	40.0
42	30.42875227553305	35.0	25.0	39.0	7.0	40.0
43	30.322401707434455	35.0	25.0	39.0	7.0	40.0
44	30.42750308635517	35.0	25.0	39.0	7.0	40.0
45	30.480992237032076	35.0	25.0	39.0	7.0	40.0
46	30.31558243183863	35.0	24.0	39.0	7.0	40.0
47	30.25664664895064	35.0	25.0	39.0	7.0	40.0
48	30.115279027431942	35.0	24.0	38.0	7.0	40.0
49	30.273842355255173	35.0	26.0	39.0	7.0	40.0
50	30.259103178422716	35.0	25.0	39.0	7.0	40.0
51	29.021334560900588	34.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	8.0
9	27.0
10	33.0
11	35.0
12	26.0
13	54.0
14	82.0
15	111.0
16	215.0
17	474.0
18	906.0
19	1535.0
20	2452.0
21	3583.0
22	5321.0
23	7710.0
24	11038.0
25	15876.0
26	20171.0
27	22095.0
28	20955.0
29	20460.0
30	21645.0
31	25051.0
32	29767.0
33	35206.0
34	39219.0
35	45184.0
36	50817.0
37	52419.0
38	39707.0
39	5723.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.415852357138377	2.6664016237366868	35.72618275407503	35.1915632650499
2	34.48117846456446	14.70402377016593	35.28321232031136	15.531585444958257
3	13.326149274968092	14.874976460002928	56.42966248875311	15.369211776275868
4	12.172794040718964	3.1116737461028228	66.85128999183947	17.864242221338746
5	20.893055177753133	3.61197715050951	56.67133979201105	18.82362787972631
6	15.43282207947103	12.805549161976105	59.52920005858844	12.232428699964428
7	58.33022954112699	1.253583310665188	37.78849574187609	2.627691406331736
8	57.57632190161327	8.88075160595091	30.42957879098575	3.1133477014500635
9	54.493314640831954	2.613881274716997	29.453453579125778	13.43935050532527
10	33.02358184595426	17.2923772258375	36.258709798916115	13.425331129292125
11	27.024753614697328	16.629281663911616	41.45403946349731	14.891925257893746
12	21.487100081605323	15.509614781025716	45.62783787742462	17.37544725994434
13	19.36682638990605	16.000502186604173	50.70347973467808	13.9291916888117
14	16.6571111715595	20.40907283798205	45.489527316858826	17.44428867359963
15	14.647109288359733	15.704839823397712	53.17088991651148	16.477160971731077
16	17.655834780607226	17.948986210792828	43.53518444895482	20.85999455964512
17	17.557489903956814	17.873030486911762	47.341340419744306	17.22813918938712
18	18.08353037182733	17.221024879161348	46.08503693163985	18.61040781737147
19	17.24425100960432	20.175765311460317	42.793203741290206	19.786779937645164
20	20.068213680400078	20.116130652214853	46.51315101169676	13.30250465568831
21	19.485049486304952	22.830030758929507	43.99510368060932	13.689816074156221
22	17.327948776966373	15.863237848130401	47.02747379213659	19.78133958276663
23	17.87135653156452	21.251072377644327	46.11809754974786	14.759473541043292
24	19.61561800338976	17.554351237680734	43.89487560419326	18.935155154736247
25	17.160134753405455	24.67724048461007	40.98303027766734	17.179594484317132
26	16.648741394823293	17.067648720470384	45.5332594003055	20.75035048440083
27	19.269736979766066	18.3917474001381	42.61450900797221	19.72400661212362
28	14.049507229394656	20.760812705321086	45.91241028645561	19.27726977882865
29	20.191249398422297	20.87464166893348	41.32723734594379	17.606871586700425
30	20.120734029419765	20.29608085204327	44.27779288987466	15.305392228662301
31	20.597183570128266	17.768826766546002	43.487267477140044	18.146722186185684
32	25.008683643363817	20.19020317633027	39.15548952731686	15.645623652989057
33	19.86482810571028	18.821744679960663	37.55728065953841	23.75614655479065
34	20.102529765018517	22.90222008327928	41.42600071143102	15.569249440271179
35	23.154150363039065	22.784206231298782	36.10993701743006	17.951706388232093
36	18.64535163524513	28.663974388483187	35.48827185034839	17.202402125923292
37	23.906802536042353	23.729363269234792	35.50961478102572	16.854219413697137
38	20.9568747253667	23.853654453767444	33.976271682952856	21.213199137912998
39	23.991337281078025	23.23240777552259	34.565503965181726	18.210750978217657
40	24.403339540917745	21.59276851289992	34.43723713669938	19.566654809482955
41	19.258437781172187	27.11933209181645	34.02063149965475	19.601598627356616
42	21.56472976083363	22.93925634533699	36.62279508694105	18.873218806888325
43	21.664748592831288	23.87457889560796	32.36236948379402	22.098303027766732
44	22.808060094996964	21.926094871419306	33.096817392396055	22.16902764118767
45	20.22179908350945	21.128036659622104	33.87185871816869	24.778305538699755
46	23.61365110585675	24.183005168337136	31.677094013517188	20.526249712288923
47	17.376493482036366	22.682513443953884	39.634450001046226	20.306543072963528
48	19.85583059571886	23.890899960243562	33.78816095080664	22.465108493230943
49	20.145424870791572	21.535644786675316	37.46563160427696	20.853298738256157
50	19.409093762423886	21.71454876441171	35.719486932686074	23.156870540478334
51	19.395702119645957	21.338536544537675	33.83503170052939	25.43072963528698
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	59.0
1	724.5
2	1390.0
3	14856.5
4	28323.0
5	20654.0
6	12985.0
7	11911.5
8	10838.0
9	10505.5
10	10173.0
11	9746.5
12	9320.0
13	8848.0
14	8376.0
15	7865.0
16	7354.0
17	6816.5
18	6279.0
19	5844.5
20	5410.0
21	5062.0
22	4714.0
23	4614.5
24	4515.0
25	4579.0
26	4961.5
27	5280.0
28	5801.0
29	6322.0
30	6743.0
31	7164.0
32	7828.0
33	8492.0
34	9407.5
35	10323.0
36	10776.0
37	11229.0
38	11811.5
39	12394.0
40	13007.0
41	13620.0
42	14248.0
43	14876.0
44	16449.5
45	18023.0
46	40511.5
47	63000.0
48	45081.5
49	27163.0
50	25961.0
51	24759.0
52	23037.0
53	21315.0
54	21407.5
55	21500.0
56	21242.5
57	20985.0
58	20340.0
59	19695.0
60	18118.5
61	16542.0
62	14526.0
63	12510.0
64	10894.5
65	9279.0
66	7847.5
67	6416.0
68	5307.5
69	4199.0
70	3591.5
71	2984.0
72	2510.5
73	2037.0
74	1742.5
75	1229.0
76	1010.0
77	770.0
78	530.0
79	371.5
80	213.0
81	167.0
82	121.0
83	91.0
84	61.0
85	42.0
86	23.0
87	16.5
88	10.0
89	7.5
90	5.0
91	3.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	477910.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.636484244054316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.91468915564104	39.95962414054062
2	7.886372935134202	7.986763977453188
3	4.432374321422623	6.733195574714027
4	2.8517274116469924	5.776058005928028
5	1.9486871163938964	4.933733223293557
6	1.276053816958916	3.8768927398203306
7	0.8272362000836266	2.932183296815214
8	0.5238206799216327	2.1219550084449255
9	0.33791969843537645	1.539995893702067
>10	0.9142955220318206	7.248050298097941
>50	0.053012207098153755	1.859600554570421
>100	0.02880190778996535	2.7063338825315473
>500	4.1741895347775874E-4	0.13527442087005548
>1k	0.002921932674344311	2.7621345811404456
>5k	4.1741895347775874E-4	1.7184078776149236
>10k+	0.0012522568604332763	7.709796524462726
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13238	2.7699776108472305	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	12378	2.590027411018811	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG	10860	2.27239438387981	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC	8130	1.7011571216337804	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT	2704	0.565796907367496	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	2112	0.4419242116716537	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC	2059	0.4308342574961813	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGCTGAT	1845	0.38605595195748155	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT	1825	0.3818710635893788	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTA	1389	0.29064049716473817	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT	1134	0.23728317047142766	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGT	640	0.133916427779289	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04184888368102781	0.0
2	0.0	0.0	0.0	0.5557531752840493	0.0
3	0.0	0.0	0.0	0.6543072963528698	0.0
4	0.0	0.0	0.0	1.3475340545290955	0.0
5	0.0	0.0	0.0	4.4307505597288195	0.0
6	0.0	0.0	0.0	4.800276202632295	0.0
7	0.0	0.0	0.0	5.338662091188717	0.0
8	0.0	0.0	0.0	5.9417045050323285	0.0
9	0.0	0.0	0.0	6.07980582117972	0.0
10	0.0	0.0	0.0	8.866313741080956	0.0
11	0.0	0.0	0.0	9.063421983218598	0.0
12	0.0	0.0	0.0	11.21047896047373	0.0
13	0.0	0.0	0.0	11.39126613797577	0.0
14	0.0	0.0	0.0	11.53585403109372	0.0
15	4.184888368102781E-4	0.0	0.0	11.949948735117491	0.0
16	4.184888368102781E-4	0.0	0.0	12.172375551882153	0.0
17	4.184888368102781E-4	0.0	0.0	12.293737314557134	0.0
18	4.184888368102781E-4	0.0	0.0	12.386014103073801	0.0
19	4.184888368102781E-4	0.0	0.0	12.955577409972589	0.0
20	4.184888368102781E-4	0.0	0.0	13.094934192630411	0.0
21	4.184888368102781E-4	0.0	0.0	13.191186625096776	0.0
22	4.184888368102781E-4	0.0	0.0	13.391433533510494	0.0
23	4.184888368102781E-4	0.0	0.0	13.499612897825951	0.0
24	4.184888368102781E-4	0.0	0.0	13.603188884936495	0.0
25	4.184888368102781E-4	0.0	0.0	13.691908518340274	0.0
26	4.184888368102781E-4	0.0	0.0	13.807202192881505	0.0
27	4.184888368102781E-4	0.0	0.0	13.966855684124626	0.0
28	4.184888368102781E-4	0.0	0.0	14.05536607311	0.0
29	4.184888368102781E-4	0.0	0.0	14.171915214161663	0.0
30	6.277332552154172E-4	0.0	0.0	14.302274486828065	0.0
31	6.277332552154172E-4	0.0	0.0	14.408152162541064	0.0
32	6.277332552154172E-4	0.0	0.0	14.529932414052855	0.0
33	6.277332552154172E-4	0.0	0.0	14.65192190998305	0.0
34	6.277332552154172E-4	0.0	0.0	14.81513255633906	0.0
35	6.277332552154172E-4	0.0	0.0	14.966625515264381	0.0
36	6.277332552154172E-4	0.0	0.0	15.105773053503798	0.0
37	6.277332552154172E-4	0.0	0.0	15.254964323826663	0.0
38	6.277332552154172E-4	0.0	0.0	15.436588479002323	0.0
39	6.277332552154172E-4	0.0	0.0	15.737063463832103	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACCTA	20	7.0291443E-4	45.000004	23
GCGATTA	25	3.887373E-5	45.0	9
ATACCGC	25	3.887373E-5	45.0	28
CGTTTTT	9820	0.0	44.106415	1
TACGGCT	960	0.0	42.89062	7
ACGGCTG	970	0.0	42.680412	8
TGATACC	1370	0.0	42.043793	4
GATACCT	1360	0.0	42.022057	5
ACCGGTG	70	0.0	41.785717	17
GAATCTG	1610	0.0	41.226707	1
ATACCTG	1395	0.0	40.96774	6
CGATGAA	310	0.0	40.64516	19
CGGCTGT	1055	0.0	40.308056	9
ATGATAC	3925	0.0	40.24204	3
GAATGAT	4095	0.0	40.054943	1
AATCTGT	1635	0.0	40.04587	2
CACGACC	180	0.0	40.000004	27
CCGATGA	315	0.0	40.0	18
AATGATA	3915	0.0	39.88506	2
CGACCAA	170	0.0	39.705883	29
>>END_MODULE